>SLG130 (SLG130Q) /CSM/SL/SLG1-B/SLG130Q.Seq.d/
XXXXXXXXXXACCACCACCACCAGAAGTTTGTTCATTAAGATGCCCACCAAAACATGAAT
GTAAATTTGATGATCATGGTAAAAAATGTTGTGTAAAGATTCATTGTGATGAAGTTTGTG
ATTTAGATTGTGGTAGAGGTTTTGAATGTAAAATTAGACATGATGGTTCAAAATGTTGTG
TCCGTTCAGAAAGACCACACCCACCACAACATGAAAAATGTAATAAGAGATGCCCACCAG
GCCATGAATGTAAAGTTGATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCAC
CACCAAAATGCTCTTTAAGATGTCCACCAAGACATGAATGTAGAGTCAACCACTTTGGTG
AAGAATGTTGTGTTAAAGTTCACCACGATAAATGTTCATTGAGATGTCCACCAGGCCATG
AATGTAAAGTTGATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAA
AATGCTCATTAAGATGTCCACCAAAACATGAATGTAGAATCAATCACTTTGGTGAAGAAT
GCTGTGTTAAAAGTAGAAATGATTGTTTAACTTGTGAAGACCTAAACTGTGAAAGAAAAG
GTTTACATTGTGCCATGAAAACTGTACCAATTGATAAAGAAAATTGTTGTGAAAAAGTTC
CAGTATGTTATTCCACTAATCCATTATTAGATGGTGGTCATGGTTTTATTTAAAG

---PPPPEVCSLRCPPKHECKFDDHGKKCCVKIHCDEVCDLDCGRGFECKIRHDGSKCCV
RSERPHPPQHEKCNKRCPPGHECKVDQHGKECCVVAHRPPPKCSLRCPPRHECRVNHFGE
ECCVKVHHDKCSLRCPPGHECKVDQHGKECCVVAHRPPPKCSLRCPPKHECRINHFGEEC
CVKSRNDCLTCEDLNCERKGLHCAMKTVPIDKENCCEKVPVCYSTNPLLDGGHGFI*

Frame A:
---PPPPEVCSLRCPPKHECKFDDHGKKCCVKIHCDEVCDLDCGRGFECKIRHDGSKCCV
RSERPHPPQHEKCNKRCPPGHECKVDQHGKECCVVAHRPPPKCSLRCPPRHECRVNHFGE
ECCVKVHHDKCSLRCPPGHECKVDQHGKECCVVAHRPPPKCSLRCPPKHECRINHFGEEC
CVKSRNDCLTCEDLNCERKGLHCAMKTVPIDKENCCEKVPVCYSTNPLLDGGHGFI*


Frame B:
---hhhqkfvh*dahqnmnvnlmimvknvv*rfivmkfvi*ivvevlnvkldmmvqnvvs
vqkdhthhnmknvirdahqamnvklinmeknvallpidhhqnal*dvhqdmnvesttlvk
nvvlkfttinvh*dvhqamnvklinmeknvallpidhhqnah*dvhqnmnvesitlvkna
vlkvemiv*lvkt*tvkekvyivp*klyqlikkivvkkfqyviplihy*mvvmvlfk


Frame C:
---tttrslfikmptkt*m*i**sw*kmlckdsl**sl*frlw*rf*m*n*t*wfkmlcp
frkttpttt*km**emptrp*m*s*stwkrmlrccp*tttkmlfkmstkt*m*sqplw*r
mlc*sspr*mfiemstrp*m*s*stwkrmlrccp*tttkmlikmstkt*m*nqslw*rml
c*k*k*lfnl*rpkl*kkrftlchenctn**rkll*ksssmlfh*siirwwswfylk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLG130 (SLG130Q) /CSM/SL/SLG1-B/SLG130Q.Seq.d/                       1398   0.0
SLE706 (SLE706Q) /CSM/SL/SLE7-A/SLE706Q.Seq.d/                       1398   0.0
SSF378 (SSF378Q) /CSM/SS/SSF3-D/SSF378Q.Seq.d/                       1392   0.0
SLH866 (SLH866Q) /CSM/SL/SLH8-C/SLH866Q.Seq.d/                       1382   0.0
SLB753 (SLB753Q) /CSM/SL/SLB7-C/SLB753Q.Seq.d/                       1372   0.0
AHF349 (AHF349Q) /CSM/AH/AHF3-C/AHF349Q.Seq.d/                       1340   0.0
CFC513 (CFC513Q) /CSM/CF/CFC5-A/CFC513Q.Seq.d/                       1334   0.0
SFI482 (SFI482Q) /CSM/SF/SFI4-D/SFI482Q.Seq.d/                       1332   0.0
AHD802 (AHD802Q) /CSM/AH/AHD8-A/AHD802Q.Seq.d/                       1326   0.0
AFF122 (AFF122Q) /CSM/AF/AFF1-A/AFF122Q.Seq.d/                       1326   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(U25144) Dictyostelium discoideum spore coat protein SP87 (P...  1398   0.0    1
(AU039658) Dictyostelium discoideum slug cDNA, clone SLG130.     1398   0.0    1
(AU039532) Dictyostelium discoideum slug cDNA, clone SLE706.     1398   0.0    1
(C92815) Dictyostelium discoideum slug cDNA, clone SSF378.       1392   0.0    1
(AC117267) Dictyostelium discoideum chromosome 2 map 5836255...  1390   0.0    1
(AU034110) Dictyostelium discoideum slug cDNA, clone SLB753.     1372   0.0    1
(AU039480) Dictyostelium discoideum slug cDNA, clone SLH866.     1336   0.0    2
(BJ402714) Dictyostelium discoideum cDNA clone:dds17d22, 3' ...  1324   0.0    1
(BJ374239) Dictyostelium discoideum cDNA clone:ddc6i03, 3' e...  1324   0.0    1
(BJ350917) Dictyostelium discoideum cDNA clone:dda41n08, 3' ...  1322   0.0    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...   552   e-156
(P15270) RecName: Full=Spore coat protein SP60; Flags: Precursor...   187   3e-46
X52105_1(X52105|pid:none) Dictyostelium discoideum SP60 gene for...   110   5e-23
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...    89   2e-16
S07638(S07638;A60942;B60942) spore coat protein SP96 precursor -...    76   9e-13
(P14328) RecName: Full=Spore coat protein SP96;  &AC117075_49(AC...    76   9e-13
(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;        74   3e-12
(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...    72   2e-11
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...    70   5e-11
(P54704) RecName: Full=Spore coat protein SP85; AltName: Full=Ce...    63   8e-09

 psg: 0.49  gvh: 0.31  alm: 0.56  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 28.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for SLG130 is nuc

>SLG130Z.Seq
----------ACCACCACCACCAGAAGTTTGTTCATTAAGATGCCCACCAAAACATGAAT
GTAAATTTGATGATCATGGTAAAAAATGTTGTGTAAAGATTCATTGTGATGAAGTTTGTG
ATTTAGATTGTGGTAGAGGTTTTGAATGTAAAATTAGACATGATGGTTCAAAATGTTGTG
TCCGTTCAGAAAGACCACACCCACCACAACATGAAAAATGTAATAAGAGATGCCCACCAG
GCCATGAATGTAAAGTTGATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCAC
CACCAAAATGCTCTTTAAGATGTCCACCAAGACATGAATGTAGAGTCAACCACTTTGGTG
AAGAATGTTGTGTTAAAGTTCACCACGATAAATGTTCATTGAGATGTCCACCAGGCCATG
AATGTAAAGTTGATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAA
AATGCTCATTAAGATGTCCACCAAAACATGAATGTAGAATCAATCACTTTGGTGAAGAAT
GCTGTGTTAAAAGTAGAAATGATTGTTTAACTTGTGAAGACCTAAACTGTGAAAGAAAAG
GTTTACATTGTGCCATGAAAACTGTACCAATTGATAAAGAAAATTGTTGTGAAAAAGTTC
CAGTATGTTATTCCACTAATCCATTATTAGATGGTGGTCATGGTTTTATTTAAAG