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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLF442
Library SL
(Link to library)
Clone ID SLF442
Atlas ID
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16022-1
Original site URL
Representative seq. ID SLF442P
(Link to Original site)
Representative DNA sequence 
>SLF442 (SLF442Q) /CSM/SL/SLF4-B/SLF442Q.Seq.d/
TACATATCAAAATAAAAAATTAATTTTATATGTATTTTTATTAATAAAATTTTTTTTTTT
TCTCTCTTATTTTTTAAATAATAATAATAATAAAAAAAAAAAAAAAAAGAAATAAAAATG
TTAAGAGGTATTGAAATTAGAGGAAAAGTTGATAAAAATGTTAAATCAATATTAACAGAA
GAGTGTTTAACATTTTTAGGAGAATTAGAAAGAAGATTTGGAGGTATTAGAAAGAACTTA
TTACAAAAGAGATTAGATAGACAAATTGATATTAATAATGGTATTTTACCAAGTXXXXXX
XXXXTTAGAGCTGGTCATGACGGTACCTGGGTTGCCCATCCAGCTTTAATTCCAATTGCA
ATGGAACAATTTAATACTCATATGAAATCTCAAAATCAAATTTCATACATTCCATCATCA
ACTGCTCCAAATTTTGAAGAGATTGCTAAATCATTGTTATCAGTCGACCCTGTTAAACCA
GGTGATATCACTGAGGATGGCTTTAGAAGTAATATCGTTGTTGGTATTCTATATCTTGAA
GCTTGGTTAAATGGTAATGGTTGTGTACCAATTCATAATCTTATGGAAGATGCTGCTACC
GCTGAAATTTCAAGATCTCAAATTTGGCAATGGATTAAACATAAAGCACCATTACCTGAT
GGTAAATCATTCGTTACCCTTCAATATTACAATAGAATCTTTAAAGAGGAATCTGATAAA
TTGGAAAAAGCTCATCCAAATTCTAAAACTTTAACTGATGCCATTCAAATCTTTAATAAT
TTAATTGTCTCTCCAAATTTCATTGACTTTTTAACACCTTCTTGTTATAAATTTGTTGTC
GATTCTGAAAGAAAAACTTTTTCTCCAAAATTAT
sequence update 1999. 2. 9
Translated Amino Acid sequence 
yisk*kinficifinkifffsllffk*****KKKKKEIKMLRGIEIRGKVDKNVKSILTE
ECLTFLGELERRFGGIRKNLLQKRLDRQIDINNGILPS---

---RAGHDGTWVAHPALIPIAMEQFNTHMKSQNQISYIPSSTAPNFEEIAKSLLSVDPVK
PGDITEDGFRSNIVVGILYLEAWLNGNGCVPIHNLMEDAATAEISRSQIWQWIKHKAPLP
DGKSFVTLQYYNRIFKEESDKLEKAHPNSKTLTDAIQIFNNLIVSPNFIDFLTPSCYKFV
VDSERKTFSPKL
Translated Amino Acid sequence (All Frames) 
Frame A:
yisk*kinficifinkifffsllffk*****KKKKKEIKMLRGIEIRGKVDKNVKSILTE
ECLTFLGELERRFGGIRKNLLQKRLDRQIDINNGILPS---

---lelvmtvpglpiql*fqlqwnnlili*nlkikfhtfhhqllqilkrllnhcyqstll
nqvislrmalevisllvfyilklg*mvmvvyqfiilwkmllplkfqdlkfgnglnikhhy
lmvnhslpfnitieslkrnlinwkkliqilkl*lmpfkslii*lslqisltf*hllvinl
lsilkeklflqny

Frame B:
tyqnkklilyvfllikfffflsyflnnnnnkkkkkkk*kc*evlkleeklikmlnqy*qk
sv*hf*en*kedlevlertyykrd*idklilimvfyq---

---*sws*rylgcpssfnsncngti*ysyeisksnfihsiincskf*rdc*iivisrpc*
tr*yh*gwl*k*yrcwysis*slvkw*wlctns*sygrccyr*nfkisnlamd*t*stit
*w*iirypsilq*nl*rgi**igkssskf*nfn*chsnl**fnclskfh*lfntfll*ic
crf*kknffski

Frame C:
hikikn*fymyfy**nffffslif*iiiiikkkkkrnknvkry*n*rks**kc*ininrr
vfnifrrirkkiwry*kelitkeir*tn*y**wyftk---

---RAGHDGTWVAHPALIPIAMEQFNTHMKSQNQISYIPSSTAPNFEEIAKSLLSVDPVK
PGDITEDGFRSNIVVGILYLEAWLNGNGCVPIHNLMEDAATAEISRSQIWQWIKHKAPLP
DGKSFVTLQYYNRIFKEESDKLEKAHPNSKTLTDAIQIFNNLIVSPNFIDFLTPSCYKFV
VDSERKTFSPKL
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLF442 (SLF442Q) /CSM/SL/SLF4-B/SLF442Q.Seq.d/                       1459   0.0
SFF709 (SFF709Q) /CSM/SF/SFF7-A/SFF709Q.Seq.d/                       1090   0.0
CFF652 (CFF652Q) /CSM/CF/CFF6-C/CFF652Q.Seq.d/                       1082   0.0
VFL317 (VFL317Q) /CSM/VF/VFL3-A/VFL317Q.Seq.d/                       1078   0.0
VFI785 (VFI785Q) /CSM/VF/VFI7-D/VFI785Q.Seq.d/                       1072   0.0
VFF367 (VFF367Q) /CSM/VF/VFF3-C/VFF367Q.Seq.d/                       1057   0.0
AFK696 (AFK696Q) /CSM/AF/AFK6-D/AFK696Q.Seq.d/                       1041   0.0
VSD839 (VSD839Q) /CSM/VS/VSD8-B/VSD839Q.Seq.d/                       1039   0.0
SFC185 (SFC185Q) /CSM/SF/SFC1-D/SFC185Q.Seq.d/                       1025   0.0
VFJ180 (VFJ180Q) /CSM/VF/VFJ1-D/VFJ180Q.Seq.d/                       1015   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC115598) Dictyostelium discoideum chromosome 2 map 581427-...  1130   0.0    1
(AU052986) Dictyostelium discoideum slug cDNA, clone SLF442.     1130   0.0    1
(BJ398322) Dictyostelium discoideum cDNA clone:dds12b03, 3' ...  1072   0.0    3
(BJ372426) Dictyostelium discoideum cDNA clone:ddc12g14, 3' ...  1072   0.0    2
(BJ432499) Dictyostelium discoideum cDNA clone:ddv18j21, 3' ...  1072   0.0    1
(BJ433809) Dictyostelium discoideum cDNA clone:ddv23b05, 3' ...  1065   0.0    2
(BJ428336) Dictyostelium discoideum cDNA clone:ddv11f17, 3' ...  1057   0.0    1
(BJ343396) Dictyostelium discoideum cDNA clone:dda22o24, 3' ...  1023   0.0    2
(AU264636) Dictyostelium discoideum vegetative cDNA clone:VS...  1023   0.0    2
(BJ432818) Dictyostelium discoideum cDNA clone:ddv19o19, 3' ...  1015   0.0    1
dna update 2009. 5. 8
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q8T2K9) RecName: Full=Malate synthase;          EC=2.3.3.9;  &A...   390   e-107
FJ197318_1(FJ197318|pid:none) Legionella drancourtii malate synt...   158   2e-37
(P28345) RecName: Full=Malate synthase, glyoxysomal;          EC...   154   5e-36
CP001291_3955(CP001291|pid:none) Cyanothece sp. PCC 7424, comple...   153   6e-36
CP000909_2910(CP000909|pid:none) Chloroflexus aurantiacus J-10-f...   150   4e-35
CP001337_3665(CP001337|pid:none) Chloroflexus aggregans DSM 9485...   149   9e-35
CP000473_5633(CP000473|pid:none) Solibacter usitatus Ellin6076, ...   148   2e-34
EF530214_1(EF530214|pid:none) Tuber borchii malate synthase mRNA...   148   3e-34
CP001339_2521(CP001339|pid:none) Thioalkalivibrio sp. HL-EbGR7, ...   146   8e-34
CU633899_532(CU633899|pid:none) Podospora anserina genomic DNA c...   146   8e-34
protein update 2009. 6. 3
PSORT 

 psg: 0.56  gvh: 0.35  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.14  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for SLF442 is cyt
5' end seq. ID SLF442F
5' end seq. 
>SLF442F.Seq
TACATATCAAAATAAAAAATTAATTTTATATGTATTTTTATTAATAAAATTTTTTTTTTT
TCTCTCTTATTTTTTAAATAATAATAATAATAAAAAAAAAAAAAAAAAGAAATAAAAATG
TTAAGAGGTATTGAAATTAGAGGAAAAGTTGATAAAAATGTTAAATCAATATTAACAGAA
GAGTGTTTAACATTTTTAGGAGAATTAGAAAGAAGATTTGGAGGTATTAGAAAGAACTTA
TTACAAAAGAGATTAGATAGACAAATTGATATTAATAATGGTATTTTACCAAGT------
----
Length of 5' end seq. 294
3' end seq. ID SLF442Z
3' end seq. 
>SLF442Z.Seq
----------TTAGAGCTGGTCATGACGGTACCTGGGTTGCCCATCCAGCTTTAATTCCA
ATTGCAATGGAACAATTTAATACTCATATGAAATCTCAAAATCAAATTTCATACATTCCA
TCATCAACTGCTCCAAATTTTGAAGAGATTGCTAAATCATTGTTATCAGTCGACCCTGTT
AAACCAGGTGATATCACTGAGGATGGCTTTAGAAGTAATATCGTTGTTGGTATTCTATAT
CTTGAAGCTTGGTTAAATGGTAATGGTTGTGTACCAATTCATAATCTTATGGAAGATGCT
GCTACCGCTGAAATTTCAAGATCTCAAATTTGGCAATGGATTAAACATAAAGCACCATTA
CCTGATGGTAAATCATTCGTTACCCTTCAATATTACAATAGAATCTTTAAAGAGGAATCT
GATAAATTGGAAAAAGCTCATCCAAATTCTAAAACTTTAACTGATGCCATTCAAATCTTT
AATAATTTAATTGTCTCTCCAAATTTCATTGACTTTTTAACACCTTCTTGTTATAAATTT
GTTGTCGATTCTGAAAGAAAAACTTTTTCTCCAAAATTAT
Length of 3' end seq. 570
Connected seq. ID SLF442P
Connected seq. 
>SLF442P.Seq
TACATATCAAAATAAAAAATTAATTTTATATGTATTTTTATTAATAAAATTTTTTTTTTT
TCTCTCTTATTTTTTAAATAATAATAATAATAAAAAAAAAAAAAAAAAGAAATAAAAATG
TTAAGAGGTATTGAAATTAGAGGAAAAGTTGATAAAAATGTTAAATCAATATTAACAGAA
GAGTGTTTAACATTTTTAGGAGAATTAGAAAGAAGATTTGGAGGTATTAGAAAGAACTTA
TTACAAAAGAGATTAGATAGACAAATTGATATTAATAATGGTATTTTACCAAGT------
----TTAGAGCTGGTCATGACGGTACCTGGGTTGCCCATCCAGCTTTAATTCCAATTGCA
ATGGAACAATTTAATACTCATATGAAATCTCAAAATCAAATTTCATACATTCCATCATCA
ACTGCTCCAAATTTTGAAGAGATTGCTAAATCATTGTTATCAGTCGACCCTGTTAAACCA
GGTGATATCACTGAGGATGGCTTTAGAAGTAATATCGTTGTTGGTATTCTATATCTTGAA
GCTTGGTTAAATGGTAATGGTTGTGTACCAATTCATAATCTTATGGAAGATGCTGCTACC
GCTGAAATTTCAAGATCTCAAATTTGGCAATGGATTAAACATAAAGCACCATTACCTGAT
GGTAAATCATTCGTTACCCTTCAATATTACAATAGAATCTTTAAAGAGGAATCTGATAAA
TTGGAAAAAGCTCATCCAAATTCTAAAACTTTAACTGATGCCATTCAAATCTTTAATAAT
TTAATTGTCTCTCCAAATTTCATTGACTTTTTAACACCTTCTTGTTATAAATTTGTTGTC
GATTCTGAAAGAAAAACTTTTTCTCCAAAATTAT
Length of connected seq. 864
Full length Seq ID -
Full length Seq. -
Length of full length seq. -