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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLF323
Library SL
(Link to library)
Clone ID SLF323
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1
Original site URL
Representative seq. ID SLF323E
(Link to Original site)
Representative DNA sequence 
>SLF323 (SLF323Q) /CSM/SL/SLF3-A/SLF323Q.Seq.d/
CTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTG
CCGCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAA
TGTGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGA
GACAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCG
GTGATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATAAATAAATAATATTTCTTAAT
AAATAGCATTTTATAATAATTTT
sequence update 1998. 9.10
Translated Amino Acid sequence 
EIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPMCVESFTEYPPLGRFAVRDMR
QTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK*innis**iafynnf
Translated Amino Acid sequence (All Frames) 
Frame A:
lkssiklivvlvpslpkkvlppss*rmvmplwsn*phlvqcvlnpslnthh*vvspsei*
dkpspsvssnqpsrkpqvkpvirkvllphqkrnk*iiflnk*hfiii


Frame B:
*nrr*s*ssywcrrcqrrycrrrlkew*crygrinpissnvc*ilh*iptirsfrrqrye
tnrrrrchqinrqespr*sr**ercccpikkeink*yflinsil**f


Frame C:
EIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPMCVESFTEYPPLGRFAVRDMR
QTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK*innis**iafynnf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI414 (VSI414Q) /CSM/VS/VSI4-A/VSI414Q.Seq.d/                        529   e-150
VSF761 (VSF761Q) /CSM/VS/VSF7-C/VSF761Q.Seq.d/                        529   e-150
VSE380 (VSE380Q) /CSM/VS/VSE3-D/VSE380Q.Seq.d/                        529   e-150
VSD740 (VSD740Q) /CSM/VS/VSD7-B/VSD740Q.Seq.d/                        529   e-150
VSD380 (VSD380Q) /CSM/VS/VSD3-D/VSD380Q.Seq.d/                        529   e-150
VSD312 (VSD312Q) /CSM/VS/VSD3-A/VSD312Q.Seq.d/                        529   e-150
VFO616 (VFO616Q) /CSM/VF/VFO6-A/VFO616Q.Seq.d/                        529   e-150
VFO348 (VFO348Q) /CSM/VF/VFO3-B/VFO348Q.Seq.d/                        529   e-150
VFN666 (VFN666Q) /CSM/VF/VFN6-C/VFN666Q.Seq.d/                        529   e-150
VFN474 (VFN474Q) /CSM/VF/VFN4-D/VFN474Q.Seq.d/                        529   e-150
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(X55973) D. discoideum EF1-I gene for elongation factor 1 al...   531   e-147  1
(AU285051) Dictyostelium discoideum gamete cDNA clone:FCL-AC...   531   e-147  1
(AU285021) Dictyostelium discoideum gamete cDNA clone:FCL-AA...   531   e-147  1
(AU285015) Dictyostelium discoideum gamete cDNA clone:FCL-AA...   531   e-147  1
(AU284628) Dictyostelium discoideum gamete cDNA clone:FC-BF1...   531   e-147  1
(AU284420) Dictyostelium discoideum gamete cDNA clone:FC-AT2...   531   e-147  1
(AU284342) Dictyostelium discoideum gamete cDNA clone:FC-AN0...   531   e-147  1
(AU284225) Dictyostelium discoideum gamete cDNA clone:FC-AA2...   531   e-147  1
(AU268657) Dictyostelium discoideum vegetative cDNA clone:VS...   531   e-147  1
(AU266030) Dictyostelium discoideum vegetative cDNA clone:VS...   531   e-147  1
dna update 2009. 5. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   142   5e-33
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   142   5e-33
(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   142   5e-33
FJ539138_1(FJ539138|pid:none) Ignatius tetrasporus strain UTEX B...    99   6e-20
(Q00251) RecName: Full=Elongation factor 1-alpha;          Short...    98   8e-20
AF398148_1(AF398148|pid:none) Brassica rapa subsp. pekinensis tr...    98   1e-19
FN392319_1522(FN392319|pid:none) Pichia pastoris GS115 chromosom...    98   1e-19
AY445082_1(AY445082|pid:none) Metarhizium anisopliae strain E6 t...    97   2e-19
(P40911) RecName: Full=Elongation factor 1-alpha;          Short...    97   2e-19
AJ970312_1(AJ970312|pid:none) Hebeloma cylindrosporum mRNA for e...    96   3e-19
protein update 2009. 7. 5
PSORT 

 psg: 0.69  gvh: 0.34  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.13  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 44.0 %: cytoplasmic
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SLF323 is nuc
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID SLF323E
Full length Seq. 
>SLF323E.Seq
CTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTG
CCGCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAA
TGTGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGA
GACAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCG
GTGATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATAAATAAATAATATTTCTTAAT
AAATAGCATTTTATAATAATTTT
Length of full length seq. 323