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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLF242
Library SL
(Link to library)
Clone ID SLF242
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10844-1|Contig-U15422-1
Original site URL
Representative seq. ID SLF242P
(Link to Original site)
Representative DNA sequence 
>SLF242 (SLF242Q) /CSM/SL/SLF2-B/SLF242Q.Seq.d/
ATCGATGATGCACGTGCTTTCCTTGGTATTCCATATGCCAAACCACCAACTGGACCTCTT
CGTTTCAAACCACCAGTTTCCAAACCATACTCATTCGGTGTCCAAGTTGCCGCCTCCAAT
CCAAAATCATGTTGGCAATCAGGTGCCACCGCTGCCAATTCAGCCAACTACAGTGAAGAT
TGTTTATATTTGAATGTTTTCACACCAAAATATAAACAAAGAACTCAAGGTCTTATTTTC
TCAGATTTACCAGTXXXXXXXXXXTAGCCGCTTCTGAATTCTTTGGTGATTATTACATTA
CTTGTGGTTCAATCTTAGCCGCTCAATACTTTAACGCTTACGATACAGACTTCAAGACTT
ACATCTTTAATTACTCTTCACCAAACTATCCAGCCAGTGAAGCTTATTTAGCTGCTTCCC
ATGGTAACGAATTAGCTTACATTTTCTTCAATCCAAACATTTACACTCAATTCGTTTTCG
GCGCTGCTGATTCTCTCATGAGTCTTCGTATGAATAGAGCTTGGACTGATTTCGCTGATT
CTGGTAATCCAATCTCACCATTATCAGAATGGCCAACTAACTATCCATCAGCTATGTACT
ACGGTCCAGATGCCAATAGTTTTGGTGATAGTCGTCCATACTTAAAAGATATTTGCGAAT
CATGGAGAACTTATTATGAAGCTTAAATTATCATTTATAAATAATTATTAGAAATTTAAT
AAATTTCATTACTAAAAAAAATAACAAAGCGAAATGTTGTTNTTNATCATTTAAATGTAA
CAATAAAAAAAAAAAATTTTAAAAGTTGTAAAAAAAAAAAAAAAAAAAAGGGCGCCNNTT
CTGG
sequence update 1999. 2.12
Translated Amino Acid sequence 
IDDARAFLGIPYAKPPTGPLRFKPPVSKPYSFGVQVAASNPKSCWQSGATAANSANYSED
CLYLNVFTPKYKQRTQGLIFSDLP---

---AASEFFGDYYITCGSILAAQYFNAYDTDFKTYIFNYSSPNYPASEAYLAASHGNELA
YIFFNPNIYTQFVFGAADSLMSLRMNRAWTDFADSGNPISPLSEWPTNYPSAMYYGPDAN
SFGDSRPYLKDICESWRTYYEA*iiiyk*llei**isllkkitkrnvvxxhlnvtikkkn
fksckkkkkkkgaxs
Translated Amino Acid sequence (All Frames) 
Frame A:
IDDARAFLGIPYAKPPTGPLRFKPPVSKPYSFGVQVAASNPKSCWQSGATAANSANYSED
CLYLNVFTPKYKQRTQGLIFSDLP---

---*pllnslviitllvvqs*plntltltiqtsrltslitlhqtiqpvkli*llpmvtn*
ltfssiqtftlnsfsallils*vfv*ielglislilviqshhyqngqltihqlcttvqmp
ivlvivvht*kifanhgelimklklsfinny*kfnkfhy*kk*qsemlxxii*m*q*kkk
ilkvvkkkkkkrapxl

Frame B:
smmhvlslvfhmpnhqldlfvsnhqfpnhthsvsklpppiqnhvgnqvpplpiqpttvki
vyi*mfshqninkelkvlfsqiyq---

---srf*ilw*llhylwfnlsrsil*rlryrlqdlhl*llftklssq*slfscfpw*ris
lhflqskhlhsirfrrc*fshessye*sld*fr*fw*snltiirman*lsisyvlrsrcq
*fw**ssilkrylrimenll*slnyhl*iiirnlinfitkknnkakccxxsfkcnnkkkk
f*kl*kkkkkkgrxfw

Frame C:
r*ctcfpwysicqttnwtssfqttsfqtilircpscrlqskimlairchrcqfsqlq*rl
fifecfhtki*tknsrsyflrfts---

---AASEFFGDYYITCGSILAAQYFNAYDTDFKTYIFNYSSPNYPASEAYLAASHGNELA
YIFFNPNIYTQFVFGAADSLMSLRMNRAWTDFADSGNPISPLSEWPTNYPSAMYYGPDAN
SFGDSRPYLKDICESWRTYYEA*iiiyk*llei**isllkkitkrnvvxxhlnvtikkkn
fksckkkkkkkgaxs
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLF242 (SLF242Q) /CSM/SL/SLF2-B/SLF242Q.Seq.d/                       1435   0.0
AFF821 (AFF821Q) /CSM/AF/AFF8-A/AFF821Q.Seq.d/                        938   0.0
SLF235 (SLF235Q) /CSM/SL/SLF2-B/SLF235Q.Seq.d/                        862   0.0
VFB880 (VFB880Q) /CSM/VF/VFB8-D/VFB880Q.Seq.d/                        805   0.0
VHD258 (VHD258Q) /CSM/VH/VHD2-C/VHD258Q.Seq.d/                        668   0.0
VHH282 (VHH282Q) /CSM/VH/VHH2-D/VHH282Q.Seq.d/                        385   e-106
VFF891 (VFF891Q) /CSM/VF/VFF8-D/VFF891Q.Seq.d/                        256   5e-67
CFF126 (CFF126Q) /CSM/CF/CFF1-B/CFF126Q.Seq.d/                        145   1e-33
AFF106 (AFF106Q) /CSM/AF/AFF1-A/AFF106Q.Seq.d/                        139   8e-32
VFI132 (VFI132Q) /CSM/VF/VFI1-B/VFI132Q.Seq.d/                         54   4e-06
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU052873) Dictyostelium discoideum slug cDNA, clone SLF242.      930   0.0    2
(BJ340209) Dictyostelium discoideum cDNA clone:dda12j06, 3' ...   930   0.0    2
(AU052868) Dictyostelium discoideum slug cDNA, clone SLF235.      829   0.0    2
(BJ429812) Dictyostelium discoideum cDNA clone:ddv4p20, 3' e...   805   0.0    2
(BJ438303) Dictyostelium discoideum cDNA clone:ddv37c16, 3' ...   668   0.0    1
(AU061654) Dictyostelium discoideum slug cDNA, clone SLF242.      504   e-138  1
(BJ441132) Dictyostelium discoideum cDNA clone:ddv45c22, 3' ...   377   e-105  2
(BJ428676) Dictyostelium discoideum cDNA clone:ddv12f24, 3' ...   252   2e-66  2
(BJ372098) Dictyostelium discoideum cDNA clone:ddc11c07, 3' ...   145   2e-34  2
(BJ339969) Dictyostelium discoideum cDNA clone:dda11k01, 3' ...   139   2e-28  1
dna update 2009. 5. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC107806_1(BC107806|pid:none) Rattus norvegicus similar to Carbo...    65   4e-09
BC102288_1(BC102288|pid:none) Bos taurus carboxylesterase 2 (int...    65   4e-09
BC117742_1(BC117742|pid:none) Mus musculus RIKEN cDNA 2310038E17...    64   6e-09
(P36196) RecName: Full=Acetylcholinesterase;          Short=AChE...    64   6e-09
BC117743_1(BC117743|pid:none) Mus musculus RIKEN cDNA 2310038E17...    64   6e-09
BC127887_1(BC127887|pid:none) Homo sapiens thyroglobulin, mRNA (...    64   8e-09
AK299197_1(AK299197|pid:none) Homo sapiens cDNA FLJ51282 complet...    64   8e-09
AB209159_1(AB209159|pid:none) Homo sapiens mRNA for Thyroglobuli...    64   8e-09
(Q64419) RecName: Full=Liver carboxylesterase;          EC=3.1.1...    64   8e-09
AF080484_1(AF080484|pid:none) Homo sapiens thyroglobulin gene, e...    64   8e-09
protein update 2009. 6. 3
PSORT 

 psg: 0.75  gvh: 0.43  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 40.0 %: nuclear
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial

>> prediction for SLF242 is cyt
5' end seq. ID SLF242F
5' end seq. 
>SLF242F.Seq
ATCGATGATGCACGTGCTTTCCTTGGTATTCCATATGCCAAACCACCAACTGGACCTCTT
CGTTTCAAACCACCAGTTTCCAAACCATACTCATTCGGTGTCCAAGTTGCCGCCTCCAAT
CCAAAATCATGTTGGCAATCAGGTGCCACCGCTGCCAATTCAGCCAACTACAGTGAAGAT
TGTTTATATTTGAATGTTTTCACACCAAAATATAAACAAAGAACTCAAGGTCTTATTTTC
TCAGATTTACCAGT----------
Length of 5' end seq. 254
3' end seq. ID SLF242Z
3' end seq. 
>SLF242Z.Seq
----------TAGCCGCTTCTGAATTCTTTGGTGATTATTACATTACTTGTGGTTCAATC
TTAGCCGCTCAATACTTTAACGCTTACGATACAGACTTCAAGACTTACATCTTTAATTAC
TCTTCACCAAACTATCCAGCCAGTGAAGCTTATTTAGCTGCTTCCCATGGTAACGAATTA
GCTTACATTTTCTTCAATCCAAACATTTACACTCAATTCGTTTTCGGCGCTGCTGATTCT
CTCATGAGTCTTCGTATGAATAGAGCTTGGACTGATTTCGCTGATTCTGGTAATCCAATC
TCACCATTATCAGAATGGCCAACTAACTATCCATCAGCTATGTACTACGGTCCAGATGCC
AATAGTTTTGGTGATAGTCGTCCATACTTAAAAGATATTTGCGAATCATGGAGAACTTAT
TATGAAGCTTAAATTATCATTTATAAATAATTATTAGAAATTTAATAAATTTCATTACTA
AAAAAAATAACAAAGCGAAATGTTGTTNTTNATCATTTAAATGTAACAATAAAAAAAAAA
AATTTTAAAAGTTGTAAAAAAAAAAAAAAAAAAAAGGGCGCCNNTTCTGG
Length of 3' end seq. 580
Connected seq. ID SLF242P
Connected seq. 
>SLF242P.Seq
ATCGATGATGCACGTGCTTTCCTTGGTATTCCATATGCCAAACCACCAACTGGACCTCTT
CGTTTCAAACCACCAGTTTCCAAACCATACTCATTCGGTGTCCAAGTTGCCGCCTCCAAT
CCAAAATCATGTTGGCAATCAGGTGCCACCGCTGCCAATTCAGCCAACTACAGTGAAGAT
TGTTTATATTTGAATGTTTTCACACCAAAATATAAACAAAGAACTCAAGGTCTTATTTTC
TCAGATTTACCAGT----------TAGCCGCTTCTGAATTCTTTGGTGATTATTACATTA
CTTGTGGTTCAATCTTAGCCGCTCAATACTTTAACGCTTACGATACAGACTTCAAGACTT
ACATCTTTAATTACTCTTCACCAAACTATCCAGCCAGTGAAGCTTATTTAGCTGCTTCCC
ATGGTAACGAATTAGCTTACATTTTCTTCAATCCAAACATTTACACTCAATTCGTTTTCG
GCGCTGCTGATTCTCTCATGAGTCTTCGTATGAATAGAGCTTGGACTGATTTCGCTGATT
CTGGTAATCCAATCTCACCATTATCAGAATGGCCAACTAACTATCCATCAGCTATGTACT
ACGGTCCAGATGCCAATAGTTTTGGTGATAGTCGTCCATACTTAAAAGATATTTGCGAAT
CATGGAGAACTTATTATGAAGCTTAAATTATCATTTATAAATAATTATTAGAAATTTAAT
AAATTTCATTACTAAAAAAAATAACAAAGCGAAATGTTGTTNTTNATCATTTAAATGTAA
CAATAAAAAAAAAAAATTTTAAAAGTTGTAAAAAAAAAAAAAAAAAAAAGGGCGCCNNTT
CTGG
Length of connected seq. 834
Full length Seq ID -
Full length Seq. -
Length of full length seq. -