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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLF225
Library SL
(Link to library)
Clone ID SLF225
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16171-1
Original site URL
Representative seq. ID SLF225P
(Link to Original site)
Representative DNA sequence 
>SLF225 (SLF225Q) /CSM/SL/SLF2-B/SLF225Q.Seq.d/
GTTAACAATTTTATTCTTAATTTTGGCTTTAATTTCAGTCANTTATGCTCAACACAAATT
CCCATCATCATCTGAAGATTCATCATCAAATGATTCAAATAGCCAAGTAACTGGTTCACA
ATCATACTCTGGXXXXXXXXXXAGTGGTATTTCAGGCGGTTCTGGTTCAGGTTCAGGTTC
AGGTTCAGGCTCAGGTAGCGGTTCAGGTTCAGGTTCAGGTGCTGTCAGTGGTTCACAATC
AGGTTCAGGTGCTGTCAGTGGTTCACAATCAGGTTCAGGTGCTGTTAGTGGTTCACAATC
TGGTGTCCAAACTGGTTCACAATCAGGTGCTGGTAGTGCTTCAGGTGCTTTCACTGGTAA
CCCATCTGGTTCTCAATCTCAAGAAATTAATACTGGTTCATCATACAGTGGTAGTGGTAG
TGGTGCCCCAACTGGTGCCGCTACTGGTTCAGGTTCAGGTTCAGGTTCATCAGGAACAGT
TTACTATTAAATAAATTTTAAATTATAACGAAATCATATTTTTCCAGTTTTCAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
sequence update 1999. 2.12
Translated Amino Acid sequence 
LTILFLILALISVXYAQHKFPSSSEDSSSNDSNSQVTGSQSYS---

---VVFQAVLVQVQVQVQAQVAVQVQVQVLSVVHNQVQVLSVVHNQVQVLLVVHNLVSKL
VHNQVLVVLQVLSLVTHLVLNLKKLILVHHTVVVVVVPQLVPLLVQVQVQVHQEQFTIK*
ilnyneiiffqfskkkkkkkkkkkkkkkkk
Translated Amino Acid sequence (All Frames) 
Frame A:
vnnfilnfgfnfsxlcstqipiii*rfiik*fk*psnwftiilw---

---sgisggsgsgsgsgsgsgsgsgsgsgavsgsqsgsgavsgsqsgsgavsgsqsgvqt
gsqsgagsasgaftgnpsgsqsqeintgssysgsgsgaptgaatgsgsgsgssgtvyy*i
nfkl*rnhifpvfkkkkkkkkkkkkkkkkkk

Frame B:
LTILFLILALISVXYAQHKFPSSSEDSSSNDSNSQVTGSQSYS---

---VVFQAVLVQVQVQVQAQVAVQVQVQVLSVVHNQVQVLSVVHNQVQVLLVVHNLVSKL
VHNQVLVVLQVLSLVTHLVLNLKKLILVHHTVVVVVVPQLVPLLVQVQVQVHQEQFTIK*
ilnyneiiffqfskkkkkkkkkkkkkkkkk

Frame C:
*qfys*fwl*fqsxmlntnshhhlkihhqmiqiak*lvhnhtl---

---wyfrrfwfrfrfrfrlr*rfrfrfrccqwftirfrccqwftirfrcc*wftiwcpnw
ftircw*cfrcfhw*piwfsisrn*ywfiiqw*w*wcpnwcrywfrfrfrfirnslllnk
f*iitksyfssfqkkkkkkkkkkkkkkkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFA868 (VFA868Q) /CSM/VF/VFA8-C/VFA868Q.Seq.d/                        618   e-176
SLF225 (SLF225Q) /CSM/SL/SLF2-B/SLF225Q.Seq.d/                        618   e-176
VHJ422 (VHJ422Q) /CSM/VH/VHJ4-A/VHJ422Q.Seq.d/                        599   e-170
VSG609 (VSG609Q) /CSM/VS/VSG6-A/VSG609Q.Seq.d/                        591   e-168
VSK169 (VSK169Q) /CSM/VS/VSK1-C/VSK169Q.Seq.d/                        587   e-167
VHQ154 (VHQ154Q) /CSM/VH/VHQ1-C/VHQ154Q.Seq.d/                        587   e-167
VSD364 (VSD364Q) /CSM/VS/VSD3-C/VSD364Q.Seq.d/                        581   e-165
VHI283 (VHI283Q) /CSM/VH/VHI2-D/VHI283Q.Seq.d/                        579   e-164
VSC419 (VSC419Q) /CSM/VS/VSC4-A/VSC419Q.Seq.d/                        551   e-156
VFB716 (VFB716Q) /CSM/VF/VFB7-A/VFB716Q.Seq.d/                        537   e-152
own update 2002.12.11
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ442208) Dictyostelium discoideum cDNA clone:ddv49l06, 3' ...   509   0.0    2
(BJ446721) Dictyostelium discoideum cDNA clone:ddv63k13, 3' ...   498   0.0    2
(BJ441765) Dictyostelium discoideum cDNA clone:ddv47e22, 3' ...   490   0.0    2
(BJ429612) Dictyostelium discoideum cDNA clone:ddv4p03, 3' e...   252   0.0    3
(AU270749) Dictyostelium discoideum vegetative cDNA clone:VS...   498   0.0    2
(BJ410987) Dictyostelium discoideum cDNA clone:ddv2h18, 5' e...   529   0.0    2
(AU270748) Dictyostelium discoideum vegetative cDNA clone:VS...   424   0.0    2
(AU266248) Dictyostelium discoideum vegetative cDNA clone:VS...   404   e-176  2
(BJ423015) Dictyostelium discoideum cDNA clone:ddv47e22, 5' ...   404   e-176  2
(BJ427796) Dictyostelium discoideum cDNA clone:ddv63k13, 5' ...   404   e-176  2
dna update 2009. 5. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000393_2258(CP000393|pid:none) Trichodesmium erythraeum IMS101...    49   1e-04
CP000828_875(CP000828|pid:none) Acaryochloris marina MBIC11017, ...    47   3e-04
BA000004_971(BA000004|pid:none) Bacillus halodurans C-125 DNA, c...    47   3e-04
(Q54T37) RecName: Full=Uncharacterized transmembrane protein DDB...    47   5e-04
CP001325_267(CP001325|pid:none) Micromonas sp. RCC299 chromosome...    46   8e-04
U00051_3(U00051|pid:none) Caenorhabditis elegans cosmid F42G9, c...    45   0.001
U00051_4(U00051|pid:none) Caenorhabditis elegans cosmid F42G9, c...    45   0.001
AM407005_1(AM407005|pid:none) Staphylococcus aureus partial clfB...    45   0.001
AM407050_1(AM407050|pid:none) Staphylococcus aureus partial clfB...    45   0.002
AM407093_1(AM407093|pid:none) Staphylococcus aureus partial clfB...    45   0.002
protein update 2009. 6. 3
PSORT 

 psg: 0.99  gvh: 0.83  alm: 0.25  top: 0.37  tms: 0.20  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 40.0 %: endoplasmic reticulum
	 24.0 %: mitochondrial
	 16.0 %: plasma membrane
	  8.0 %: extracellular, including cell wall
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for SLF225 is end
5' end seq. ID SLF225F
5' end seq. 
>SLF225F.Seq
GTTAACAATTTTATTCTTAATTTTGGCTTTAATTTCAGTCANTTATGCTCAACACAAATT
CCCATCATCATCTGAAGATTCATCATCAAATGATTCAAATAGCCAAGTAACTGGTTCACA
ATCATACTCTGG----------
Length of 5' end seq. 132
3' end seq. ID SLF225Z
3' end seq. 
>SLF225Z.Seq
----------AGTGGTATTTCAGGCGGTTCTGGTTCAGGTTCAGGTTCAGGTTCAGGCTC
AGGTAGCGGTTCAGGTTCAGGTTCAGGTGCTGTCAGTGGTTCACAATCAGGTTCAGGTGC
TGTCAGTGGTTCACAATCAGGTTCAGGTGCTGTTAGTGGTTCACAATCTGGTGTCCAAAC
TGGTTCACAATCAGGTGCTGGTAGTGCTTCAGGTGCTTTCACTGGTAACCCATCTGGTTC
TCAATCTCAAGAAATTAATACTGGTTCATCATACAGTGGTAGTGGTAGTGGTGCCCCAAC
TGGTGCCGCTACTGGTTCAGGTTCAGGTTCAGGTTCATCAGGAACAGTTTACTATTAAAT
AAATTTTAAATTATAACGAAATCATATTTTTCCAGTTTTCAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Length of 3' end seq. 444
Connected seq. ID SLF225P
Connected seq. 
>SLF225P.Seq
GTTAACAATTTTATTCTTAATTTTGGCTTTAATTTCAGTCANTTATGCTCAACACAAATT
CCCATCATCATCTGAAGATTCATCATCAAATGATTCAAATAGCCAAGTAACTGGTTCACA
ATCATACTCTGG----------AGTGGTATTTCAGGCGGTTCTGGTTCAGGTTCAGGTTC
AGGTTCAGGCTCAGGTAGCGGTTCAGGTTCAGGTTCAGGTGCTGTCAGTGGTTCACAATC
AGGTTCAGGTGCTGTCAGTGGTTCACAATCAGGTTCAGGTGCTGTTAGTGGTTCACAATC
TGGTGTCCAAACTGGTTCACAATCAGGTGCTGGTAGTGCTTCAGGTGCTTTCACTGGTAA
CCCATCTGGTTCTCAATCTCAAGAAATTAATACTGGTTCATCATACAGTGGTAGTGGTAG
TGGTGCCCCAACTGGTGCCGCTACTGGTTCAGGTTCAGGTTCAGGTTCATCAGGAACAGT
TTACTATTAAATAAATTTTAAATTATAACGAAATCATATTTTTCCAGTTTTCAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Length of connected seq. 576
Full length Seq ID -
Full length Seq. -
Length of full length seq. -