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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFK381
Library AF
(Link to library)
Clone ID AFK381
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10734-1
Original site URL
Representative seq. ID AFK381Z
(Link to Original site)
Representative DNA sequence 
>AFK381 (AFK381Q) /CSM/AF/AFK3-D/AFK381Q.Seq.d/
XXXXXXXXXXTGGGTCGATATGAAGAAATTCCCACAACAAGCGACTCATATGGGTCTCTC
TGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTT
CGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGG
TGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGT
TAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTT
GGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAA
ATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTC
AAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGC
TGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAANGATCATATGAACTTTGT
TGAATTCATTCAAGATAACGCTGCCATGAATTCAAATTACCATCATCTCAAACTGATGAT
AATGTNAATCTAAAAAAGATTCTTCTGCTAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
---VDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIGG
KVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKL
GEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRXDHMNFVE
FIQDNAAMNSNYHHLKLMIMXI*krffc*
Translated Amino Acid sequence (All Frames) 
Frame A:
---gsi*rnshnkrliwvslvklfqpslliqlpiklvtismkkkpshsipspngskmslv
vkflhllnlnqnqnqmmlqlrlplvplskswf*thqrmfwlnstlhgaviarillqstin
*vnt*ktlnlsqs*rlmptqmmyqvisklevtqpscssklmtkkiqslmkvkexii*tll
nsfkitlp*iqitiisn***cxskkdssa


Frame B:
---gryeeipttsdsygslw*scsshlc*fscq*sslqfr*krnlliryrlqmdprchww
*sfsic*istrtrik*css*gcrwyhfqkvgfrltkgcfg*ilrsmvrslqescsnlr*i
r*ilkrr*iclnrkd*crlk*ctk*yrn*rlpnhhalqs**qrksnll*rskkxsyelc*
ihsr*rchefklpssqtddnvnlkkilll


Frame C:
---VDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIGG
KVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKL
GEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRXDHMNFVE
FIQDNAAMNSNYHHLKLMIMXI*krffc*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK381 (AFK381Q) /CSM/AF/AFK3-D/AFK381Q.Seq.d/                       1219   0.0
AFO713 (AFO713Q) /CSM/AF/AFO7-A/AFO713Q.Seq.d/                       1211   0.0
AFO433 (AFO433Q) /CSM/AF/AFO4-B/AFO433Q.Seq.d/                       1211   0.0
AFM548 (AFM548Q) /CSM/AF/AFM5-B/AFM548Q.Seq.d/                       1211   0.0
AFM212 (AFM212Q) /CSM/AF/AFM2-A/AFM212Q.Seq.d/                       1211   0.0
AFK539 (AFK539Q) /CSM/AF/AFK5-B/AFK539Q.Seq.d/                       1211   0.0
AFK217 (AFK217Q) /CSM/AF/AFK2-A/AFK217Q.Seq.d/                       1211   0.0
AFJ487 (AFJ487Q) /CSM/AF/AFJ4-D/AFJ487Q.Seq.d/                       1211   0.0
AFN682 (AFN682Q) /CSM/AF/AFN6-D/AFN682Q.Seq.d/                       1205   0.0
AFK269 (AFK269Q) /CSM/AF/AFK2-C/AFK269Q.Seq.d/                       1205   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end, single read.   593  e-165  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74  2e-09  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.    70  4e-08  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70  4e-08  1
BM280519|BM280519.1 ki01h03.y1 Ascaris suum L4 pSPORT1 Zarlenga v1 Ascaris suum cDNA 5' similar to SW:ERP5_CAEEL Q11067 PROBABLE PROTEIN DISULFIDE ISOMERASE P5 PRECURSOR ;, mRNA sequence.    68  1e-07  1
AW255071|AW255071.1 ML1365 peppermint glandular trichome Mentha x piperita cDNA, mRNA sequence.    64  2e-06  1
BM315730|BM315730.1 fw64d09.y1 Sugano SJD adult male Danio rerio cDNA clone 5612584 5' similar to SW:ER60_HUMAN P30101 PROBABLE PROTEIN DISULFIDE ISOMERASE ER-60 PRECURSOR ;, mRNA sequence.    62  9e-06  1
BU795021|S34275 protein disulfide-isomerase homolog precursor - fluke  (Schistosoma mansoni), mRNA sequence.    60  4e-05  1
BM308748|BM308748.1 sak49h10.y1 Gm-c1036 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1036-6523 5' similar to TR:P93358 P93358 PROTEIN DISULFIDE-ISOMERASE PRECURSOR. ;, mRNA sequence.    60  4e-05  1
BM174369|PDA4_MOUSE PROTEIN DISULFIDE ISOMERASE A4 PRECURSOR (PROTEIN ERP-72) (ERP72), mRNA sequence.    60  4e-05  1
dna update 2003. 9. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   165   8e-40
(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   164   2e-39
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   160   2e-38
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   159   6e-38
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   154   2e-36
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   152   7e-36
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   151   2e-35
BC046707_1(BC046707|pid:none) Xenopus laevis glucose regulated p...   149   5e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   149   6e-35
(P13667) RecName: Full=Protein disulfide-isomerase A4;          ...   149   6e-35
protein update 2009. 4.24
PSORT 

 psg: 0.84  gvh: 0.38  alm: 0.43  top: 0.43  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 32.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFK381 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFK381Z
3' end seq. 
>AFK381Z.Seq
----------TGGGTCGATATGAAGAAATTCCCACAACAAGCGACTCATATGGGTCTCTC
TGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTT
CGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGG
TGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGT
TAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTT
GGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAA
ATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTC
AAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGC
TGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAANGATCATATGAACTTTGT
TGAATTCATTCAAGATAACGCTGCCATGAATTCAAATTACCATCATCTCAAACTGATGAT
AATGTNAATCTAAAAAAGATTCTTCTGCTAA
Length of 3' end seq. 621
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -