>SLE826 (SLE826Q) /CSM/SL/SLE8-B/SLE826Q.Seq.d/
XXXXXXXXXXTCATCTGCATCATCTTCATCTGCATCATCTGCAGCAACTACAGCTGCAAC
TACAATTGCAACTACAGCTGCAACTACAACTGCAACTACAACCGCAACCACAGCTACAAC
CACAGCCACAACCACAGCTACAACCACAGCTGCAACCATATCTACAACCACAGCTGCAAC
TACAACTGCAACTACAACCGCAACCACAGCTACAACCACAGCTACAACCACAGCTACCTC
TTAAATTTAATCAAAAAACATTTTTTATCAAGTATCACTACTATTATAATATTAATAATA
ATAGTATCAAAAAATGATATTAAAAATCCCCTTTTTTTCTCTATAATTTTTAAATTAAAT
ATAAAATAAAT

---ICIIFICIICSNYSCNYNCNYSCNYNCNYNRNHSYNHSHNHSYNHSCNHIYNHSCNY
NCNYNRNHSYNHSYNHSYLLNLIKKHFLSSITTIIILIIIVSKNDIKNPLFFSIIFKLNI
K*

Frame A:
---hlhhlhlhhlqqlqlqlqlqlqlqlqlqlqpqpqlqpqpqpqlqpqlqpylqpqlql
qlqlqpqpqlqpqlqpqlplkfnqktffikyhyyyninnnsikk*y*kspfflynf*iky
kin


Frame B:
---ICIIFICIICSNYSCNYNCNYSCNYNCNYNRNHSYNHSHNHSYNHSCNHIYNHSCNY
NCNYNRNHSYNHSYNHSYLLNLIKKHFLSSITTIIILIIIVSKNDIKNPLFFSIIFKLNI
K*


Frame C:
---sassssassaattaattiattaatttatttattatttatttatttaatistttaatt
tatttattatttatttats*i*sknifyqvslll*y****yqkmilkipffsl*fln*i*
nk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLE826 (SLE826Q) /CSM/SL/SLE8-B/SLE826Q.Seq.d/                        674   0.0
SLC525 (SLC525Q) /CSM/SL/SLC5-B/SLC525Q.Seq.d/                        646   0.0
SSB820 (SSB820Q) /CSM/SS/SSB8-A/SSB820Q.Seq.d/                        642   0.0
SLI557 (SLI557Q) /CSM/SL/SLI5-C/SLI557Q.Seq.d/                        642   0.0
SLH738 (SLH738Q) /CSM/SL/SLH7-B/SLH738Q.Seq.d/                        642   0.0
SLH548 (SLH548Q) /CSM/SL/SLH5-B/SLH548Q.Seq.d/                        642   0.0
SLH472 (SLH472Q) /CSM/SL/SLH4-C/SLH472Q.Seq.d/                        642   0.0
SLH386 (SLH386Q) /CSM/SL/SLH3-D/SLH386Q.Seq.d/                        642   0.0
SLH314 (SLH314Q) /CSM/SL/SLH3-A/SLH314Q.Seq.d/                        642   0.0
SLH308 (SLH308Q) /CSM/SL/SLH3-A/SLH308Q.Seq.d/                        642   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU039603) Dictyostelium discoideum slug cDNA, clone SLE826.      315   2e-91  2
(AU034287) Dictyostelium discoideum slug cDNA, clone SLC525.      291   5e-83  2
(AU052199) Dictyostelium discoideum slug cDNA, clone SLA224.      283   8e-82  2
(C25558) Dictyostelium discoideum slug cDNA, clone SLA405.        283   2e-81  2
(AU052130) Dictyostelium discoideum slug cDNA, clone SLA579.      283   2e-79  3
(AU039854) Dictyostelium discoideum slug cDNA, clone SLG594.      283   2e-79  2
(AC117075) Dictyostelium discoideum chromosome 2 map 5201047...   283   6e-79  2
(AU052384) Dictyostelium discoideum slug cDNA, clone SLD230.      283   9e-78  3
(AU053922) Dictyostelium discoideum slug cDNA, clone SLK165.      283   1e-77  2
(AU033673) Dictyostelium discoideum slug cDNA, clone SLB296.      283   1e-77  3

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3159261
Number of Hits to DB: 121,846,172

 psg: 1.00  gvh: 0.38  alm: 0.24  top: 0.43  tms: 0.07  mit: 0.51  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.06  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: mitochondrial
	 20.0 %: endoplasmic reticulum
	 12.0 %: cytoplasmic
	 12.0 %: nuclear
	  8.0 %: Golgi
	  8.0 %: plasma membrane
	  4.0 %: extracellular, including cell wall
	  4.0 %: vesicles of secretory system

>> prediction for SLE826 is mit

>SLE826Z.Seq
----------TCATCTGCATCATCTTCATCTGCATCATCTGCAGCAACTACAGCTGCAAC
TACAATTGCAACTACAGCTGCAACTACAACTGCAACTACAACCGCAACCACAGCTACAAC
CACAGCCACAACCACAGCTACAACCACAGCTGCAACCATATCTACAACCACAGCTGCAAC
TACAACTGCAACTACAACCGCAACCACAGCTACAACCACAGCTACAACCACAGCTACCTC
TTAAATTTAATCAAAAAACATTTTTTATCAAGTATCACTACTATTATAATATTAATAATA
ATAGTATCAAAAAATGATATTAAAAATCCCCTTTTTTTCTCTATAATTTTTAAATTAAAT
ATAAAATAAAT