>AFB253 (AFB253Q) /CSM/AF/AFB2-C/AFB253Q.Seq.d/
XXXXXXXXXXAAAATCAAAATCAAAATCATAATCAGAATCAAAATCCACATTCAAACTTT
CAGGTTTCTAATGATCAATTAATTGCTATAGATGTTGGTATTGCAGGTGGTGCTATCGTT
GCAGGCGCTGGTGCAGCAGCAGCCGGTGCAGCAGCCGGTGCTGAGGGTGGTGCCGCAGGA
GTTGCTTCCAGAGCTGGTGGTTATATTTCTCGTACTGCTAGTCGTGTTGGTAACTCTATA
CGTAGCAGAGCTACTCGTTATGCTAATCAAGGTTACAATTCCATGAGAAATGGTGCACAA
AGAGCTGGCAACGGCGCACGTAATTATGCTAATGAAGGTTACAATTCCGTGAGAGATGGT
GCACAAAGAGCTGGCAACGGCGCACGTAATTATGCTCAACAAGGTTATCAAGCATGGAGA
GAAGGTGTCAATGAGATTGGTGATGATATCGCCAATACTGCACATGATGTAGGTGATGGT
TTCGCCAATACTGCACGTGAAGTAGGTGATGGAATTAGAATCACTGCAGAGGAGGGATAT
GAACTCATTAAAGATGGAGCTGAAGAAACTTGGGTGAAGAAATATTAAAGGTTATCCTTA
ATTAAAATTAAAAAAGCGCAACAAAG

---NQNQNHNQNQNPHSNFQVSNDQLIAIDVGIAGGAIVAGAGAAAAGAAAGAEGGAAGV
ASRAGGYISRTASRVGNSIRSRATRYANQGYNSMRNGAQRAGNGARNYANEGYNSVRDGA
QRAGNGARNYAQQGYQAWREGVNEIGDDIANTAHDVGDGFANTAREVGDGIRITAEEGYE
LIKDGAEETWVKKY*rlslikikkaqq

Frame A:
---ksksks*seskstfklsgf**sincyrcwycrwcyrcrrwcsssrcssrc*gwcrrs
cfqswwlyfsyc*scw*lyt*qsyslc*srlqfhekwctkswqrrt*lc**rlqfrerwc
tkswqrrt*lcstrlssmerrcq*dw**yrqyct*cr*wfrqyct*sr*wn*nhcrggi*
th*rws*rnlgeeilkviln*n*ksatk


Frame B:
---NQNQNHNQNQNPHSNFQVSNDQLIAIDVGIAGGAIVAGAGAAAAGAAAGAEGGAAGV
ASRAGGYISRTASRVGNSIRSRATRYANQGYNSMRNGAQRAGNGARNYANEGYNSVRDGA
QRAGNGARNYAQQGYQAWREGVNEIGDDIANTAHDVGDGFANTAREVGDGIRITAEEGYE
LIKDGAEETWVKKY*rlslikikkaqq


Frame C:
---ikikiiirikihiqtfrflmin*ll*mlvlqvvlslqalvqqqpvqqpvlrvvpqel
lpelvviflvllvvlvtlyvaellvmlikvtip*emvhkelatahvimlmkvtip*emvh
kelatahvimlnkvikhgekvsmrlvmispilhmm*vmvspilhvk*vmeleslqrrdmn
slkmelkklg*rnikgyp*lklkkrnk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFB253 (AFB253Q) /CSM/AF/AFB2-C/AFB253Q.Seq.d/                       1150   0.0
SFK737 (SFK737Q) /CSM/SF/SFK7-B/SFK737Q.Seq.d/                       1142   0.0
SFK728 (SFK728Q) /CSM/SF/SFK7-B/SFK728Q.Seq.d/                       1142   0.0
SFH254 (SFH254Q) /CSM/SF/SFH2-C/SFH254Q.Seq.d/                       1142   0.0
SFG695 (SFG695Q) /CSM/SF/SFG6-D/SFG695Q.Seq.d/                       1142   0.0
SFF877 (SFF877Q) /CSM/SF/SFF8-D/SFF877Q.Seq.d/                       1142   0.0
SFF390 (SFF390Q) /CSM/SF/SFF3-D/SFF390Q.Seq.d/                       1142   0.0
SFF293 (SFF293Q) /CSM/SF/SFF2-D/SFF293Q.Seq.d/                       1142   0.0
SFD394 (SFD394Q) /CSM/SF/SFD3-D/SFD394Q.Seq.d/                       1142   0.0
CHD379 (CHD379Q) /CSM/CH/CHD3-D/CHD379Q.Seq.d/                       1142   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BE737326|BE737326.1 601305982F1 NIH_MGC_39 Homo sapiens cDNA clone IMAGE:3640269 5', mRNA sequence.    48  0.13  1
AL359620|AL359620.1 Homo sapiens mRNA; cDNA DKFZp762P2111 (from clone DKFZp762P2111).    46  0.51  1
CC525270|CC525270.1 CH240_399F19.T7 CHORI-240 Bos taurus genomic clone CH240_399F19, genomic survey sequence.    46  0.51  1
BM591238|BM591238.1 17000687385453 A.Gam.ad.cDNA.blood1 Anopheles gambiae cDNA clone 19600449728408 5', mRNA sequence.    42  1.2  2
BM623215|BM623215.1 17000687478121 A.Gam.ad.cDNA1 Anopheles gambiae cDNA clone 19600449656355 5', mRNA sequence.    42  1.3  2
AF350281|AF350281.1 Plectreurys tristis fibroin 1 mRNA, partial cds.    36  1.4  3
CD044849|CD044849.1 psHB016xE22f_183218 psHB: Infected hypocotyl soybean host. 48 hrs post infection Phytophthora sojae cDNA clone sHB016E22 5, mRNA sequence.    44  2.0  1
AC103427|AC103427.6 Rattus norvegicus clone CH230-149N19, *** SEQUENCING IN PROGRESS ***, 9 unordered pieces.    44  2.0  1
BG929819|BG929819.1 etsHEST0240 EtH11 Eimeria tenella cDNA clone etsHf009 5', mRNA sequence.    44  2.0  1
CD043491|CD043491.1 psHB008xN14f_180168 psHB: Infected hypocotyl soybean host. 48 hrs post infection Phytophthora sojae cDNA clone sHB008N14 5, mRNA sequence.    44  2.0  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AJ628940_1(AJ628940|pid:none) Pisum sativum mRNA for late embryo...    51   3e-05
Z69360_4(Z69360|pid:none) Caenorhabditis elegans Cosmid F25H8, c...    49   1e-04
AC146855_28(AC146855|pid:none) Medicago truncatula clone mth2-16...    49   1e-04
Z69360_3(Z69360|pid:none) Caenorhabditis elegans Cosmid F25H8, c...    49   1e-04
AM421808_498(AM421808|pid:none) Neisseria meningitidis serogroup...    49   1e-04
T21369(T21369)hypothetical protein F25H8.5a - Caenorhabditis ele...    47   4e-04
AL844509_656(AL844509|pid:none) Plasmodium falciparum 3D7 chromo...    46   7e-04
CT573213_2889(CT573213|pid:none) Frankia alni str. ACN14A chromo...    44   0.003
AB207257_1(AB207257|pid:none) Polypedilum vanderplanki PvLEA3 mR...    42   0.010
BT050559_1(BT050559|pid:none) Drosophila melanogaster AT13054 fu...    42   0.014

 psg: 0.71  gvh: 0.52  alm: 0.29  top: 0.63  tms: 0.07  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 20.0 %: nuclear
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for AFB253 is cyt

>AFB253Z.Seq
----------AAAATCAAAATCAAAATCATAATCAGAATCAAAATCCACATTCAAACTTT
CAGGTTTCTAATGATCAATTAATTGCTATAGATGTTGGTATTGCAGGTGGTGCTATCGTT
GCAGGCGCTGGTGCAGCAGCAGCCGGTGCAGCAGCCGGTGCTGAGGGTGGTGCCGCAGGA
GTTGCTTCCAGAGCTGGTGGTTATATTTCTCGTACTGCTAGTCGTGTTGGTAACTCTATA
CGTAGCAGAGCTACTCGTTATGCTAATCAAGGTTACAATTCCATGAGAAATGGTGCACAA
AGAGCTGGCAACGGCGCACGTAATTATGCTAATGAAGGTTACAATTCCGTGAGAGATGGT
GCACAAAGAGCTGGCAACGGCGCACGTAATTATGCTCAACAAGGTTATCAAGCATGGAGA
GAAGGTGTCAATGAGATTGGTGATGATATCGCCAATACTGCACATGATGTAGGTGATGGT
TTCGCCAATACTGCACGTGAAGTAGGTGATGGAATTAGAATCACTGCAGAGGAGGGATAT
GAACTCATTAAAGATGGAGCTGAAGAAACTTGGGTGAAGAAATATTAAAGGTTATCCTTA
ATTAAAATTAAAAAAGCGCAACAAAG