>SLE589 (SLE589Q) /CSM/SL/SLE5-D/SLE589Q.Seq.d/
AGTTACATTATATTTCTCAACTGATATTCATCATGAATTATTTGTAGATACAACTCGTGG
TGAAAAATTAAAAATTAATATGGATATTACTTTTCACCATTTACCATGTGCATATTTAAG
TTTAGATGCAATGGATGTTTCAGGTGAACATCAATTTGATGTAGCACATAATATATTTAA
GAAAAGATTATCACCAACAGGACAACCAATTATTGAAGCACCACCAATTAGAGAGGAGGA
GATTAATAAGAAAGAATCAGTAAAGGATAATAATGATGTTGTTGGATGTGGTAGTTGTTA
TGGTGCTGAGGATCCTTCAAAAGGTATAGGGTGCTGTAATACTTGTGAGGAGGTAAGAGT
AGCATATAGTAAAAAGGGTTGGGGTTTAGATCCAAGTGGTATTCCACAATGTATTAGAGA
AGGTTTCACAAAGAATTTAGTTGAACAAAATGGTGAAGGTTGTCAAGTATATGGTTTCAT
TCTCGTCAATAAAGTAGCTGGTAATTTCCATTTTGCACCTGGTAAATCATTCCAACAACA
TCATATGCACGTTCACGACCTTCAACCATTTAAAGATGGTTCTTTCAATGTTAGTCATAC
AATTAATAGACTTTCATTTGGTAATGATTTCCXXXXXXXXXX

VTLYFSTDIHHELFVDTTRGEKLKINMDITFHHLPCAYLSLDAMDVSGEHQFDVAHNIFK
KRLSPTGQPIIEAPPIREEEINKKESVKDNNDVVGCGSCYGAEDPSKGIGCCNTCEEVRV
AYSKKGWGLDPSGIPQCIREGFTKNLVEQNGEGCQVYGFILVNKVAGNFHFAPGKSFQQH
HMHVHDLQPFKDGSFNVSHTINRLSFGNDF---

Frame A:
syiifln*yss*iicrynsw*kikn*ygyyfspftmcifkfrcngcfr*tsi*cst*yi*
ekiitnrttny*sttn*rggd**eriskg***ccwmw*llwc*gsfkryrvl*yl*ggks
si**kglgfrskwystmy*rrfhkefs*tkw*rlssiwfhsrq*ssw*fpfctw*iiptt
syarsrpsti*rwffqc*syn**tfiw**f---


Frame B:
VTLYFSTDIHHELFVDTTRGEKLKINMDITFHHLPCAYLSLDAMDVSGEHQFDVAHNIFK
KRLSPTGQPIIEAPPIREEEINKKESVKDNNDVVGCGSCYGAEDPSKGIGCCNTCEEVRV
AYSKKGWGLDPSGIPQCIREGFTKNLVEQNGEGCQVYGFILVNKVAGNFHFAPGKSFQQH
HMHVHDLQPFKDGSFNVSHTINRLSFGNDF---


Frame C:
lhyisqlifimnyl*iqlvvkn*kliwillftiyhvhi*v*mqwmfqvninlm*hiiylr
kdyhqqdnqllkhhqlerrrlirknq*riimmlldvvvvmvlrilqkv*gavilvrr*e*
hivkrvgv*iqvvfhnvlekvsqri*lnkmvkvvkymvsfssik*lvisilhlvnhsnni
ictfttfnhlkmvlsmlviqlidfhlvmi---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLE589 (SLE589Q) /CSM/SL/SLE5-D/SLE589Q.Seq.d/                       1253   0.0
AFM671 (AFM671Q) /CSM/AF/AFM6-C/AFM671Q.Seq.d/                        504   e-142
VSB812 (VSB812Q) /CSM/VS/VSB8-A/VSB812Q.Seq.d/                        448   e-125
SSI184 (SSI184Q) /CSM/SS/SSI1-D/SSI184Q.Seq.d/                         42   0.010
SSL137 (SSL137Q) /CSM/SS/SSL1-B/SSL137Q.Seq.d/                         40   0.041
VSD628 (VSD628Q) /CSM/VS/VSD6-B/VSD628Q.Seq.d/                         36   0.63
VSC324 (VSC324Q) /CSM/VS/VSC3-A/VSC324Q.Seq.d/                         36   0.63
VHQ360 (VHQ360Q) /CSM/VH/VHQ3-C/VHQ360Q.Seq.d/                         36   0.63
VHP133 (VHP133Q) /CSM/VH/VHP1-B/VHP133Q.Seq.d/                         36   0.63
VHL480 (VHL480Q) /CSM/VH/VHL4-D/VHL480Q.Seq.d/                         36   0.63

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU061539) Dictyostelium discoideum slug cDNA, clone SLE589.     1253   0.0    1
(BJ347293) Dictyostelium discoideum cDNA clone:dda26m18, 3' ...   244   e-135  3
(AU262262) Dictyostelium discoideum vegetative cDNA clone:VS...   448   e-121  1
(DY448402) HSAA-aaa41a03.g1 UCI-11 Hydra vulgaris cDNA 5' si...    52   6e-14  4
(DN138422) ACAE-aaa14m15.g1 Hydra EST UCI 5 Hydra magnipapil...    44   3e-13  4
(CV181035) tai67e12.y2 Hydra EST UCI 5 ALP Hydra magnipapill...    44   1e-12  4
(DY447273) HSAA-aaa12d10.g1 UCI-11 Hydra vulgaris cDNA 5' si...    60   8e-09  3
(DT606426) ACAG-aaa32e07.g1 Hydra_EST_UCI-9 Hydra magnipapil...    44   3e-08  3
(CF658544) tac66h07.y1 Hydra EST -IV Hydra magnipapillata cD...    44   1e-07  3
(EE669650) SAAH-aac39h02.g1 Agen 0058 Schmidtea mediterranea...    42   1e-07  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q803I2) RecName: Full=Endoplasmic reticulum-Golgi intermediate ...   234   2e-60
BT025364_1(BT025364|pid:none) Bos taurus ERGIC and golgi 3 (ERGI...   231   1e-59
(Q5R8G3) RecName: Full=Endoplasmic reticulum-Golgi intermediate ...   231   1e-59
(Q5EAE0) RecName: Full=Endoplasmic reticulum-Golgi intermediate ...   231   1e-59
(Q6NVS2) RecName: Full=Endoplasmic reticulum-Golgi intermediate ...   229   7e-59
BC100125_1(BC100125|pid:none) Danio rerio ERGIC and golgi 3, mRN...   228   1e-58
BC129572_1(BC129572|pid:none) Xenopus laevis hypothetical protei...   228   1e-58
BT058936_1(BT058936|pid:none) Salmo salar clone ssal-rgf-514-293...   224   2e-57
AL121586_13(AL121586|pid:none) Human DNA sequence from clone RP3...   223   4e-57
AL833786_12(AL833786|pid:none) Mouse DNA sequence from clone RP2...   222   8e-57

 psg: 0.75  gvh: 0.47  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for SLE589 is cyt

>SLE589F.Seq
AGTTACATTATATTTCTCAACTGATATTCATCATGAATTATTTGTAGATACAACTCGTGG
TGAAAAATTAAAAATTAATATGGATATTACTTTTCACCATTTACCATGTGCATATTTAAG
TTTAGATGCAATGGATGTTTCAGGTGAACATCAATTTGATGTAGCACATAATATATTTAA
GAAAAGATTATCACCAACAGGACAACCAATTATTGAAGCACCACCAATTAGAGAGGAGGA
GATTAATAAGAAAGAATCAGTAAAGGATAATAATGATGTTGTTGGATGTGGTAGTTGTTA
TGGTGCTGAGGATCCTTCAAAAGGTATAGGGTGCTGTAATACTTGTGAGGAGGTAAGAGT
AGCATATAGTAAAAAGGGTTGGGGTTTAGATCCAAGTGGTATTCCACAATGTATTAGAGA
AGGTTTCACAAAGAATTTAGTTGAACAAAATGGTGAAGGTTGTCAAGTATATGGTTTCAT
TCTCGTCAATAAAGTAGCTGGTAATTTCCATTTTGCACCTGGTAAATCATTCCAACAACA
TCATATGCACGTTCACGACCTTCAACCATTTAAAGATGGTTCTTTCAATGTTAGTCATAC
AATTAATAGACTTTCATTTGGTAATGATTTCC----------