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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLE582
Library SL
(Link to library)
Clone ID SLE582
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U01267-1|Contig-U16394-1
Original site URL
Representative seq. ID SLE582P
(Link to Original site)
Representative DNA sequence 
>SLE582 (SLE582Q) /CSM/SL/SLE5-D/SLE582Q.Seq.d/
ATGTTTACATCATTTCCTCTCGATGACCGATATTGATATACGTATGATTTACATTCTATT
GGAATGTGAAGATATTCATGTCAATGTGAACAATCGTGATTTGAATACACCATTTCATTA
TTTCTGTCAAAAGTTTCGTGCACAAAATAATGGTCAATTAAAGGAGGTTTTCGATATACT
CATTAAAAAAGGTGTGAATGTCAATGCCATCAATAAGAATGGTGAATCACCACTCCACAA
AGCAATTTTCAATACTACAGTGGCTCAAGGTATGATTACATTACTCTTGGAGAATAAATC
CAATGTGAATATAGTCAATAAGAATGGTGAGTCACCACTTCACTATTGCGTTAGAATGGG
TCGTGCCGATTTGGTTTCCATACTTCTTCAATATGGCGCCGATCGTAGTGCCGTCGCCAA
AGATAATAAACCACTCTATCAAATTGCAATCGATGAAAACTTTACAAAGTTAGCCGATCT
TCTTAGAGAGGGTGGTGATATGTCAAAGATTTCCGATGGTGCCTCCACTGAACTTTTATC
TCTTCTTCAAAATCCAACACTTCGTGAAGACTTTAGGAAGTTTTTAAGAGAAGAATTAAT
CTGTGAAGAGAATATAAACTTTTATACTGACGTTGAAGCTTACAAATACTTAAXXXXXXX
XXXATGATAATAATAATAATAATAATAATAGTCAACAATCATCACCCTCTTCATCAACTC
CATCTTCACCAATTATTTCTGGTAATAATTTGGATGTTGAAAAACTTCAATCAATTTTTA
ATATTGCTCAACATCACATTTTTAATTTAATGGCAAGTGATTCTCTTGCAAAATAT
sequence update 1999. 1.21
Translated Amino Acid sequence 
CLHHFLSMTDIDIRMIYILLECEDIHVNVNNRDLNTPFHYFCQKFRAQNNGQLKEVFDIL
IKKGVNVNAINKNGESPLHKAIFNTTVAQGMITLLLENKSNVNIVNKNGESPLHYCVRMG
RADLVSILLQYGADRSAVAKDNKPLYQIAIDENFTKLADLLREGGDMSKISDGASTELLS
LLQNPTLREDFRKFLREELICEENINFYTDVEAYKYL---

---DNNNNNNNSQQSSPSSSTPSSPIISGNNLDVEKLQSIFNIAQHHIFNLMASDSLAKY
Translated Amino Acid sequence (All Frames) 
Frame A:
mftsfplddry*ytydlhsigm*ryscqceqs*feytislflskvsctk*wsikggfryt
h*krcecqchq*ew*ittpqsnfqyysgsrydyitlge*iqceysq*ew*vttsllr*ng
scrfgfhtssiwrrs*crrqr**ttlsncnr*klykvsrss*rgw*yvkdfrwclh*tfi
sssksnts*rl*evfkrrinl*reyklly*r*slqil---

---miiiiiiiivnnhhplhqlhlhqlflviiwmlknfnqflillnitfli*wqvillqn

Frame B:
CLHHFLSMTDIDIRMIYILLECEDIHVNVNNRDLNTPFHYFCQKFRAQNNGQLKEVFDIL
IKKGVNVNAINKNGESPLHKAIFNTTVAQGMITLLLENKSNVNIVNKNGESPLHYCVRMG
RADLVSILLQYGADRSAVAKDNKPLYQIAIDENFTKLADLLREGGDMSKISDGASTELLS
LLQNPTLREDFRKFLREELICEENINFYTDVEAYKYL---

---*********stiitlfinsiftnyfw**fgc*ktsinf*ycstshf*fngk*fscki

Frame C:
vyiissr*piliyv*ftfywnvkifmsm*tivi*ihhfiisvksfvhkimvn*rrfsiys
lkkv*msmpsirmvnhhstkqfsilqwlkv*lhyswrinpm*i*sirmvshhftialewv
vpiwfpyffnmapivvpspkiinhsiklqsmktlqs*piflervvicqrfpmvpplnfyl
ffkiqhfvktlgsf*ekn*svkri*tfiltlkltnt*---

---DNNNNNNNSQQSSPSSSTPSSPIISGNNLDVEKLQSIFNIAQHHIFNLMASDSLAKY
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLE582 (SLE582Q) /CSM/SL/SLE5-D/SLE582Q.Seq.d/                       1294   0.0
SSG887 (SSG887Q) /CSM/SS/SSG8-D/SSG887Q.Seq.d/                       1090   0.0
VSD669 (VSD669Q) /CSM/VS/VSD6-C/VSD669Q.Seq.d/                        848   0.0
SSG183 (SSG183Q) /CSM/SS/SSG1-D/SSG183Q.Seq.d/                        276   5e-73
CHI759 (CHI759Q) /CSM/CH/CHI7-C/CHI759Q.Seq.d/                         50   6e-05
VFO558 (VFO558Q) /CSM/VF/VFO5-C/VFO558Q.Seq.d/                         38   0.21
CHD225 (CHD225Q) /CSM/CH/CHD2-B/CHD225Q.Seq.d/                         38   0.21
VSD876 (VSD876Q) /CSM/VS/VSD8-D/VSD876Q.Seq.d/                         36   0.83
VSD701 (VSD701Q) /CSM/VS/VSD7-A/VSD701Q.Seq.d/                         36   0.83
SHL719 (SHL719Q) /CSM/SH/SHL7-A/SHL719Q.Seq.d/                         36   0.83
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU061536) Dictyostelium discoideum slug cDNA, clone SLE582.     1294   0.0    1
(AU264400) Dictyostelium discoideum vegetative cDNA clone:VS...   714   0.0    3
(AU073394) Dictyostelium discoideum slug cDNA, clone SSG887.      327   4e-85  1
(AU034851) Dictyostelium discoideum slug cDNA, clone SLE582.      289   9e-74  1
(C90010) Dictyostelium discoideum slug cDNA, clone SSG887.        281   2e-71  1
(C89810) Dictyostelium discoideum slug cDNA, clone SSG183.        276   1e-69  1
(BJ366596) Dictyostelium discoideum cDNA clone:ddc39f15, 5' ...    50   0.002  2
(AC116030) Dictyostelium discoideum chromosome 2 map 2090851...    50   0.14   1
(FC291588) CAGN6132.fwd CAGN Nematostella vectensis Nemve mi...    50   0.14   1
(FC291587) CAGN6132.rev CAGN Nematostella vectensis Nemve mi...    50   0.14   1
dna update 2009. 4.26
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54XX5) RecName: Full=Probable serine/threonine-protein kinase ...   149   1e-34
(Q54HC6) RecName: Full=Ankyrin repeat-containing protein kinase ...   132   2e-29
AY195853_1(AY195853|pid:none) Synthetic construct ankyrin repeat...    80   8e-14
AM999887_1219(AM999887|pid:none) Wolbachia endosymbiont of Culex...    79   1e-13
CP001102_210(CP001102|pid:none) Candidatus Amoebophilus asiaticu...    78   3e-13
AY195856_1(AY195856|pid:none) Synthetic construct ankyrin repeat...    77   5e-13
AY326425_1(AY326425|pid:none) Synthetic construct ankyrin repeat...    77   9e-13
CP001102_1320(CP001102|pid:none) Candidatus Amoebophilus asiatic...    76   1e-12
AY649751_1(AY649751|pid:none) Wolbachia pipientis strain wAu ank...    75   2e-12
AY195855_1(AY195855|pid:none) Synthetic construct ankyrin repeat...    74   8e-12
protein update 2009. 6. 3
PSORT 

 psg: 0.75  gvh: 0.24  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 32.0 %: nuclear
	 16.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: mitochondrial
	  4.0 %: vacuolar

>> prediction for SLE582 is cyt
5' end seq. ID SLE582F
5' end seq. 
>SLE582F.Seq
ATGTTTACATCATTTCCTCTCGATGACCGATATTGATATACGTATGATTTACATTCTATT
GGAATGTGAAGATATTCATGTCAATGTGAACAATCGTGATTTGAATACACCATTTCATTA
TTTCTGTCAAAAGTTTCGTGCACAAAATAATGGTCAATTAAAGGAGGTTTTCGATATACT
CATTAAAAAAGGTGTGAATGTCAATGCCATCAATAAGAATGGTGAATCACCACTCCACAA
AGCAATTTTCAATACTACAGTGGCTCAAGGTATGATTACATTACTCTTGGAGAATAAATC
CAATGTGAATATAGTCAATAAGAATGGTGAGTCACCACTTCACTATTGCGTTAGAATGGG
TCGTGCCGATTTGGTTTCCATACTTCTTCAATATGGCGCCGATCGTAGTGCCGTCGCCAA
AGATAATAAACCACTCTATCAAATTGCAATCGATGAAAACTTTACAAAGTTAGCCGATCT
TCTTAGAGAGGGTGGTGATATGTCAAAGATTTCCGATGGTGCCTCCACTGAACTTTTATC
TCTTCTTCAAAATCCAACACTTCGTGAAGACTTTAGGAAGTTTTTAAGAGAAGAATTAAT
CTGTGAAGAGAATATAAACTTTTATACTGACGTTGAAGCTTACAAATACTTAA-------
---
Length of 5' end seq. 653
3' end seq. ID SLE582Z
3' end seq. 
>SLE582Z.Seq
----------ATGATAATAATAATAATAATAATAATAGTCAACAATCATCACCCTCTTCA
TCAACTCCATCTTCACCAATTATTTCTGGTAATAATTTGGATGTTGAAAAACTTCAATCA
ATTTTTAATATTGCTCAACATCACATTTTTAATTTAATGGCAAGTGATTCTCTTGCAAAA
TAT
Length of 3' end seq. 173
Connected seq. ID SLE582P
Connected seq. 
>SLE582P.Seq
ATGTTTACATCATTTCCTCTCGATGACCGATATTGATATACGTATGATTTACATTCTATT
GGAATGTGAAGATATTCATGTCAATGTGAACAATCGTGATTTGAATACACCATTTCATTA
TTTCTGTCAAAAGTTTCGTGCACAAAATAATGGTCAATTAAAGGAGGTTTTCGATATACT
CATTAAAAAAGGTGTGAATGTCAATGCCATCAATAAGAATGGTGAATCACCACTCCACAA
AGCAATTTTCAATACTACAGTGGCTCAAGGTATGATTACATTACTCTTGGAGAATAAATC
CAATGTGAATATAGTCAATAAGAATGGTGAGTCACCACTTCACTATTGCGTTAGAATGGG
TCGTGCCGATTTGGTTTCCATACTTCTTCAATATGGCGCCGATCGTAGTGCCGTCGCCAA
AGATAATAAACCACTCTATCAAATTGCAATCGATGAAAACTTTACAAAGTTAGCCGATCT
TCTTAGAGAGGGTGGTGATATGTCAAAGATTTCCGATGGTGCCTCCACTGAACTTTTATC
TCTTCTTCAAAATCCAACACTTCGTGAAGACTTTAGGAAGTTTTTAAGAGAAGAATTAAT
CTGTGAAGAGAATATAAACTTTTATACTGACGTTGAAGCTTACAAATACTTAA-------
---ATGATAATAATAATAATAATAATAATAGTCAACAATCATCACCCTCTTCATCAACTC
CATCTTCACCAATTATTTCTGGTAATAATTTGGATGTTGAAAAACTTCAATCAATTTTTA
ATATTGCTCAACATCACATTTTTAATTTAATGGCAAGTGATTCTCTTGCAAAATAT
Length of connected seq. 826
Full length Seq ID -
Full length Seq. -
Length of full length seq. -