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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLE573
Library SL
(Link to library)
Clone ID SLE573
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U13335-1|Contig-U16528-1
Original site URL
Representative seq. ID SLE573P
(Link to Original site)
Representative DNA sequence 
>SLE573 (SLE573Q) /CSM/SL/SLE5-D/SLE573Q.Seq.d/
AGCAAATGGTCATATGTAATGTTANNTCTCTTTAGCTGAACAATTTGTATCTCAAAGTGA
TCCAGCTTTTTGTGGATTAGCAACATTAGCAATGGTATTAAATGCATTAAAGATTGATCC
AAATAGATTATGGAAAGGACCATGGAGATGGTTTGCAGAGGATATGTTAGATTGTTGTAT
ACCAATTGAATCGGTAAAGAAGAGAGGTATTACATTTACAGAGTTTTCATGTTTATCCAA
ATGTAATGGTGCAAATATTAAACCCTATAGAGGTGATGAGAGTGATATCAATCAATTTAG
AAATAGTATTATAGAGGCTTCAAGTAAACAAGGTATTCATTTGGTAATGTCATATAGTAG
AAAGGTTTTAGGTCAAACGGGGTCTGGTCATTATTCACCCATTGGTGGATACCATAAAGA
AAGAGATTTAGCATTGGTTTTGGATGTTGCCAGATTTAAATATTCACCACATTGGGTACC
GGTTGAAGTATTATGGGAATCAATGAAAGCATTGGATAATGAAACTAACAGACCAAGAGG
TTACTATCTAATTAGTAAGAATCCATCTTATGAACCTTCATTTTGTAGAATAAAGAATAC
ATTATCTTGGTCAAGTATTGCTGATCAATTCTTAAXXXXXXXXXXAATAATAATAATAAT
AATGATAAATATAATAAATGGGAGAAATTTTTTGAACAAATTACAAATAATTCTTTAGCA
TTTAAAGTTATAAATAAATTAATTCAAGATGGAAGAATTAAATGGAAAGTAACACATCAA
CATAATCAACATGGAACTACACAAGAAATTTGTCATGATTATCCAACTCAATTGGCAACT
TTTATGTTTTTGGCTTTCCCATCACATGTCTATAGTCGTTTATCGACAACTATTCGTGAT
AAATTGGCTGAAATTAGAAATTTAGATGGTTTAAATGCAAATGTAATCGCTGAAATAATG
AAGGTACGTGAAGAAATGTTTTTGGTTGGTAGACGTGCTTGTGAATGTTCTCCTGAAGAT
CAAATTGGTTGTGGTTCTCAAAAATCTTCAAATTAAGA
sequence update 1999. 1.21
Translated Amino Acid sequence 
anghm*CXXSLAEQFVSQSDPAFCGLATLAMVLNALKIDPNRLWKGPWRWFAEDMLDCCI
PIESVKKRGITFTEFSCLSKCNGANIKPYRGDESDINQFRNSIIEASSKQGIHLVMSYSR
KVLGQTGSGHYSPIGGYHKERDLALVLDVARFKYSPHWVPVEVLWESMKALDNETNRPRG
YYLISKNPSYEPSFCRIKNTLSWSSIADQFL---

---NNNNNNDKYNKWEKFFEQITNNSLAFKVINKLIQDGRIKWKVTHQHNQHGTTQEICH
DYPTQLATFMFLAFPSHVYSRLSTTIRDKLAEIRNLDGLNANVIAEIMKVREEMFLVGRR
ACECSPEDQIGCGSQKSSN*
Translated Amino Acid sequence (All Frames) 
Frame A:
skwsyvmlxlfs*ticisk*ssflwisnisngikcikd*sk*imertmemvcrgyvrlly
tn*igkeeryyiyrvfmfiqm*wcky*tl*r**e*yqsi*k*yyrgfk*trysfgnvi**
kgfrsngvwslfthwwip*rkrfsigfgccqi*ifttlgtg*simginesig**n*qtkr
llsn**esil*tfil*nkeyiilvkyc*sil---

---NNNNNNDKYNKWEKFFEQITNNSLAFKVINKLIQDGRIKWKVTHQHNQHGTTQEICH
DYPTQLATFMFLAFPSHVYSRLSTTIRDKLAEIRNLDGLNANVIAEIMKVREEMFLVGRR
ACECSPEDQIGCGSQKSSN*

Frame B:
anghm*CXXSLAEQFVSQSDPAFCGLATLAMVLNALKIDPNRLWKGPWRWFAEDMLDCCI
PIESVKKRGITFTEFSCLSKCNGANIKPYRGDESDINQFRNSIIEASSKQGIHLVMSYSR
KVLGQTGSGHYSPIGGYHKERDLALVLDVARFKYSPHWVPVEVLWESMKALDNETNRPRG
YYLISKNPSYEPSFCRIKNTLSWSSIADQFL---

---iiiiiminiingrnflnklqiil*hlkl*in*fkmeelngk*hiniinmelhkkfvm
iiqlnwqllcfwlshhmsivvyrqlfvinwlklei*mv*mqm*slk**ryvkkcfwlvdv
lvnvllkiklvvvlknlqik

Frame C:
qmvicnvxsl*lnnlylkviqlfvd*qh*qwy*mh*rliqidygkdhgdglqric*ivvy
qlnr*rrevlhlqsfhvypnvmvqilnpievmrvisinleivl*rlqvnkvfiw*chive
rf*vkrglviihplvdtikkei*hwfwmlpdlnihhigyrlkyygnq*khwimkltdqev
ti*lvrihlmnlhfve*rihylgqvllins*---

---*******i**mgeif*tnyk*ffsi*syk*insrwkn*mesntst*stwnytrnls*
lsnsignfyvfgfpitcl*sfidnys**ig*n*kfrwfkckcnr*nnegt*rnvfgw*tc
l*mfs*rsnwlwfskifklr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLE573 (SLE573Q) /CSM/SL/SLE5-D/SLE573Q.Seq.d/                       1247   0.0
VHA169 (VHA169Q) /CSM/VH/VHA1-C/VHA169Q.Seq.d/                        446   e-124
SLB767 (SLB767Q) /CSM/SL/SLB7-C/SLB767Q.Seq.d/                         40   0.068
VSD253 (VSD253Q) /CSM/VS/VSD2-C/VSD253Q.Seq.d/                         38   0.27
VHQ182 (VHQ182Q) /CSM/VH/VHQ1-D/VHQ182Q.Seq.d/                         38   0.27
VHP848 (VHP848Q) /CSM/VH/VHP8-B/VHP848Q.Seq.d/                         38   0.27
VHP835 (VHP835Q) /CSM/VH/VHP8-B/VHP835Q.Seq.d/                         38   0.27
VHP676 (VHP676Q) /CSM/VH/VHP6-D/VHP676Q.Seq.d/                         38   0.27
VHP631 (VHP631Q) /CSM/VH/VHP6-B/VHP631Q.Seq.d/                         38   0.27
VHP219 (VHP219Q) /CSM/VH/VHP2-A/VHP219Q.Seq.d/                         38   0.27
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU061531) Dictyostelium discoideum slug cDNA, clone SLE573.     1247   0.0    1
(AU034844) Dictyostelium discoideum slug cDNA, clone SLE573.      751   0.0    1
(BJ436584) Dictyostelium discoideum cDNA clone:ddv31i17, 3' ...   446   e-121  1
(BJ418087) Dictyostelium discoideum cDNA clone:ddv31i17, 5' ...   264   6e-66  1
(FC068883) sT7aVVM023B18079 VVM Vitis vinifera cDNA clone VV...    44   9e-06  3
(DW493668) GH_RMIRS_098_B02_F Cotton Normalized Library rand...    42   4e-04  3
(DW493669) GH_RMIRS_098_B02_R Cotton Normalized Library rand...    42   4e-04  3
(CO126507) GR__Eb10J04.f GR__Eb Gossypium raimondii cDNA clo...    42   6e-04  3
(AC160408) Mus musculus 10 BAC RP23-116I16 (Roswell Park Can...    42   0.028  2
(AC161939) Mus musculus 10 BAC RP24-364P6 (Roswell Park Canc...    42   0.029  2
dna update 2009. 4.26
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB085626_1(AB085626|pid:none) Thlaspi japonicum TjPCS mRNA for p...   231   2e-59
AY540104_1(AY540104|pid:none) Thlaspi caerulescens phytochelatin...   231   3e-59
FJ406049_1(FJ406049|pid:none) Thlaspi caerulescens phytochelatin...   231   4e-59
AB085627_1(AB085627|pid:none) Thlaspi caerulescens TcPCS mRNA fo...   231   4e-59
AP008211_1182(AP008211|pid:none) Oryza sativa (japonica cultivar...   230   5e-59
AM265632_1(AM265632|pid:none) Brassica napus pcs1 gene for phyto...   230   7e-59
AJ278627_1(AJ278627|pid:none) Brassica juncea mRNA for phytochel...   229   9e-59
(Q2TSC7) RecName: Full=Glutathione gamma-glutamylcysteinyltransf...   228   2e-58
AF411075_1(AF411075|pid:none) Glycine max homo-phytochelatin syn...   228   3e-58
(Q9S7Z3) RecName: Full=Glutathione gamma-glutamylcysteinyltransf...   228   3e-58
protein update 2009. 6. 3
PSORT 

 psg: 0.75  gvh: 0.60  alm: 0.33  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for SLE573 is cyt
5' end seq. ID SLE573F
5' end seq. 
>SLE573F.Seq
AGCAAATGGTCATATGTAATGTTANNTCTCTTTAGCTGAACAATTTGTATCTCAAAGTGA
TCCAGCTTTTTGTGGATTAGCAACATTAGCAATGGTATTAAATGCATTAAAGATTGATCC
AAATAGATTATGGAAAGGACCATGGAGATGGTTTGCAGAGGATATGTTAGATTGTTGTAT
ACCAATTGAATCGGTAAAGAAGAGAGGTATTACATTTACAGAGTTTTCATGTTTATCCAA
ATGTAATGGTGCAAATATTAAACCCTATAGAGGTGATGAGAGTGATATCAATCAATTTAG
AAATAGTATTATAGAGGCTTCAAGTAAACAAGGTATTCATTTGGTAATGTCATATAGTAG
AAAGGTTTTAGGTCAAACGGGGTCTGGTCATTATTCACCCATTGGTGGATACCATAAAGA
AAGAGATTTAGCATTGGTTTTGGATGTTGCCAGATTTAAATATTCACCACATTGGGTACC
GGTTGAAGTATTATGGGAATCAATGAAAGCATTGGATAATGAAACTAACAGACCAAGAGG
TTACTATCTAATTAGTAAGAATCCATCTTATGAACCTTCATTTTGTAGAATAAAGAATAC
ATTATCTTGGTCAAGTATTGCTGATCAATTCTTAA----------
Length of 5' end seq. 635
3' end seq. ID SLE573Z
3' end seq. 
>SLE573Z.Seq
----------AATAATAATAATAATAATGATAAATATAATAAATGGGAGAAATTTTTTGA
ACAAATTACAAATAATTCTTTAGCATTTAAAGTTATAAATAAATTAATTCAAGATGGAAG
AATTAAATGGAAAGTAACACATCAACATAATCAACATGGAACTACACAAGAAATTTGTCA
TGATTATCCAACTCAATTGGCAACTTTTATGTTTTTGGCTTTCCCATCACATGTCTATAG
TCGTTTATCGACAACTATTCGTGATAAATTGGCTGAAATTAGAAATTTAGATGGTTTAAA
TGCAAATGTAATCGCTGAAATAATGAAGGTACGTGAAGAAATGTTTTTGGTTGGTAGACG
TGCTTGTGAATGTTCTCCTGAAGATCAAATTGGTTGTGGTTCTCAAAAATCTTCAAATTA
AGA
Length of 3' end seq. 413
Connected seq. ID SLE573P
Connected seq. 
>SLE573P.Seq
AGCAAATGGTCATATGTAATGTTANNTCTCTTTAGCTGAACAATTTGTATCTCAAAGTGA
TCCAGCTTTTTGTGGATTAGCAACATTAGCAATGGTATTAAATGCATTAAAGATTGATCC
AAATAGATTATGGAAAGGACCATGGAGATGGTTTGCAGAGGATATGTTAGATTGTTGTAT
ACCAATTGAATCGGTAAAGAAGAGAGGTATTACATTTACAGAGTTTTCATGTTTATCCAA
ATGTAATGGTGCAAATATTAAACCCTATAGAGGTGATGAGAGTGATATCAATCAATTTAG
AAATAGTATTATAGAGGCTTCAAGTAAACAAGGTATTCATTTGGTAATGTCATATAGTAG
AAAGGTTTTAGGTCAAACGGGGTCTGGTCATTATTCACCCATTGGTGGATACCATAAAGA
AAGAGATTTAGCATTGGTTTTGGATGTTGCCAGATTTAAATATTCACCACATTGGGTACC
GGTTGAAGTATTATGGGAATCAATGAAAGCATTGGATAATGAAACTAACAGACCAAGAGG
TTACTATCTAATTAGTAAGAATCCATCTTATGAACCTTCATTTTGTAGAATAAAGAATAC
ATTATCTTGGTCAAGTATTGCTGATCAATTCTTAA----------AATAATAATAATAAT
AATGATAAATATAATAAATGGGAGAAATTTTTTGAACAAATTACAAATAATTCTTTAGCA
TTTAAAGTTATAAATAAATTAATTCAAGATGGAAGAATTAAATGGAAAGTAACACATCAA
CATAATCAACATGGAACTACACAAGAAATTTGTCATGATTATCCAACTCAATTGGCAACT
TTTATGTTTTTGGCTTTCCCATCACATGTCTATAGTCGTTTATCGACAACTATTCGTGAT
AAATTGGCTGAAATTAGAAATTTAGATGGTTTAAATGCAAATGTAATCGCTGAAATAATG
AAGGTACGTGAAGAAATGTTTTTGGTTGGTAGACGTGCTTGTGAATGTTCTCCTGAAGAT
CAAATTGGTTGTGGTTCTCAAAAATCTTCAAATTAAGA
Length of connected seq. 1048
Full length Seq ID -
Full length Seq. -
Length of full length seq. -