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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLE532
Library SL
(Link to library)
Clone ID SLE532
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15315-1
Original site URL
Representative seq. ID SLE532E
(Link to Original site)
Representative DNA sequence 
>SLE532 (SLE532Q) /CSM/SL/SLE5-B/SLE532Q.Seq.d/
AAGATGGTGAAGTTCCAACCGGTTGTTGTCCATGTACTCTCCCACCAACCAAACCAGATT
GCTCCCTNGTTAAATGTGCTAGACCAGTATGTAAACCATATTATAGATTAAACATGACCG
ATTCTTGTTGTGGTCGTTGTGAACCATGTACAGGTGTCGCATGTACTCTTCAAATNAAAT
ATTGTAAAGATGGTGAAGTTCCAACTGGTTGTTGTCCATGTACACCTCAACCAACTAAAA
AACCAGATTGTTCAAAAGTTCCATGTCCAAAGATTTTAAAATATTGCCAAGAAGGTGAAC
TTCCAACTGGTTGTTGTCCATGTACACCTCAACCAACTAAAAAACCAGATTGTTCAAGAG
TTCCATGTCCAAAGATTNTGAAATACTGTAAAGAAGGTGAACTTCCAACTGGTTGTTGTC
CATGTACACCTCAACCAACTAAAAAACCAGATTGTTCTGATGTAATGTGTACAATGGACA
TTAGATATTGTAAAAATGGTGAACTTCCAACTGGTTGTTGTCCTTGCACACCTCAAGAAA
CCAAAGTTCCAGATTGTTCTAAAGCTATGTGCACAATGGATATTAAATATTGTAAACCAG
GTGAAAAACCCTTTGGTTGTTGCCCATGTCGTGAAAATTTAACTCAATAAAAAATTAATA
AAATATATATATTTGTTTTTTTAATT
sequence update 1999. 7.16
Translated Amino Acid sequence 
DGEVPTGCCPCTLPPTKPDCSLVKCARPVCKPYYRLNMTDSCCGRCEPCTGVACTLQXKY
CKDGEVPTGCCPCTPQPTKKPDCSKVPCPKILKYCQEGELPTGCCPCTPQPTKKPDCSRV
PCPKIXKYCKEGELPTGCCPCTPQPTKKPDCSDVMCTMDIRYCKNGELPTGCCPCTPQET
KVPDCSKAMCTMDIKYCKPGEKPFGCCPCRENLTQ*kinkiyifvfli
Translated Amino Acid sequence (All Frames) 
Frame A:
kmvkfqpvvvhvlshqpnqiapxlnvldqyvnhiid*t*pilvvvvvnhvqvshvlfkxn
ivkmvkfqlvvvhvhlnqlknqivqkfhvqrf*niakkvnfqlvvvhvhlnqlknqivqe
fhvqrx*ntvkkvnfqlvvvhvhlnqlknqivlm*cvqwtldivkmvnfqlvvvlahlkk
pkfqivlklcaqwilnivnqvknplvvahvvki*lnkklikyiylff*


Frame B:
rw*ssnrllsmysptnqtrllpx*mc*tsm*til*ikhdrfllwsl*tmyrcrmyssnxi
l*rw*ssnwllsmytstn*ktrlfkssmskdfkilprr*tsnwllsmytstn*ktrlfks
smskdxeil*rr*tsnwllsmytstn*ktrlf*cnvyngh*il*kw*tsnwllslhtsrn
qssrlf*syvhngy*il*tr*ktlwllpms*kfnsikn**niyicffn


Frame C:
DGEVPTGCCPCTLPPTKPDCSLVKCARPVCKPYYRLNMTDSCCGRCEPCTGVACTLQXKY
CKDGEVPTGCCPCTPQPTKKPDCSKVPCPKILKYCQEGELPTGCCPCTPQPTKKPDCSRV
PCPKIXKYCKEGELPTGCCPCTPQPTKKPDCSDVMCTMDIRYCKNGELPTGCCPCTPQET
KVPDCSKAMCTMDIKYCKPGEKPFGCCPCRENLTQ*kinkiyifvfli
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLE532 (SLE532Q) /CSM/SL/SLE5-B/SLE532Q.Seq.d/                       1320   0.0
AHE311 (AHE311Q) /CSM/AH/AHE3-A/AHE311Q.Seq.d/                       1296   0.0
SSF516 (SSF516Q) /CSM/SS/SSF5-A/SSF516Q.Seq.d/                       1207   0.0
CFB787 (CFB787Q) /CSM/CF/CFB7-D/CFB787Q.Seq.d/                       1185   0.0
AFI796 (AFI796Q) /CSM/AF/AFI7-D/AFI796Q.Seq.d/                        952   0.0
FC-AB20 (FC-AB20Q) /CSM/FC/FC-AB/FC-AB20Q.Seq.d/                      924   0.0
SFK717 (SFK717Q) /CSM/SF/SFK7-A/SFK717Q.Seq.d/                        841   0.0
SSJ589 (SSJ589Q) /CSM/SS/SSJ5-D/SSJ589Q.Seq.d/                        833   0.0
SLK756 (SLK756Q) /CSM/SL/SLK7-C/SLK756Q.Seq.d/                        309   3e-83
SHJ326 (SHJ326Q) /CSM/SH/SHJ3-B/SHJ326Q.Seq.d/                         40   0.043
own update 2004. 8. 7
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC116960) Dictyostelium discoideum chromosome 2 map complem...  1320   0.0    1
(AU061519) Dictyostelium discoideum slug cDNA, clone SLE532.     1312   0.0    1
(AU034811) Dictyostelium discoideum slug cDNA, clone SLE532.     1285   0.0    1
(BJ350196) Dictyostelium discoideum cDNA clone:dda39f03, 3' ...  1227   0.0    2
(C92862) Dictyostelium discoideum slug cDNA, clone SSF516.       1207   0.0    1
(BJ373775) Dictyostelium discoideum cDNA clone:ddc4n21, 3' e...  1162   0.0    2
(BJ332424) Dictyostelium discoideum cDNA clone:dda39f03, 5' ...  1045   0.0    1
(M11012) Slime mold (D.discoideum) prestalk D11 gene, comple...   418   0.0    9
(BJ327055) Dictyostelium discoideum cDNA clone:dda18p23, 5' ...   791   0.0    2
(AU284234) Dictyostelium discoideum gamete cDNA clone:FC-AB2...   920   0.0    1
dna update 2009. 4. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YPDOD1(A23754)prestalk D11 protein precursor - slime mold (Dicty...   242   9e-63
AF196974_1(AF196974|pid:none) Carcinus maenas metallothionein Ib...    39   0.15
S59073(S59073)metallothionein isoform IIa - blue crab                  38   0.33
(P55950) RecName: Full=Metallothionein-2B;          Short=MT-2B;...    38   0.33
CP000481_1900(CP000481|pid:none) Acidothermus cellulolyticus 11B...    37   0.73
AY057395_1(AY057395|pid:none) Portunus pelagicus metallothionein...    36   1.2
AY057397_1(AY057397|pid:none) Scylla serrata metallothionein mRN...    35   2.1
X00821_1(X00821|pid:none) G.gallus type III collagen gene (exon ...    35   2.8
(P12105) RecName: Full=Collagen alpha-1(III) chain; Flags: Precu...    35   2.8
U58608_1(U58608|pid:none) Delarbrea michieana maturase (matK) ge...    35   2.8
protein update 2009. 3. 4
PSORT 

 psg: 0.75  gvh: 0.41  alm: 0.54  top: 0.53  tms: 0.00  mit: 0.06  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for SLE532 is nuc
5' end seq. ID SLE532F
5' end seq. 
>SLE532F.Seq
AAGATGGTGAAGTTCCAACCGGTTGTTGTCCATGTACTCTCCCACCAACCAAACCAGATT
GCTCCCTTGTTAAATGTGCTAGACCAGTATGTAAACCATATTATAGATTAAACATGACCG
ATTCTTGTTGTGGTCGTTGTGAACCATGTACAGGTGTCGCATGTACTCTTCAAATCAAAT
ATTGTAAAGATGGTGAAGTTCCAACTGGTTGTTGTCCATGTACACCTCAACCAACTAAAA
AACCAGATTGTTCAAAAGTTCCATGTCCAAAGATTTTAAAATATTGCCAAGAAGGTGAAC
TTCCAACTGGTTGTTGTCCATGTACACCTCAACCAACTAAAAAACCAGATTGTTCAAGAG
TTCCATGTCCAAAGATTTTGAAATACTGTAAAGAAGGTGAACTTCCAACTGGTTGTTGTC
CATGTACACCTCAACCAACTAAAAAACCAGATTGTTCTGATGTAATGTGTACAATGGACA
TTAGATATTGTAAAAATGGTGAACTTCCAACTGGTTGTTGTCCTTGCACACCTCAAGAAA
CCAAAGTTCCAGATTGTTCTAAAGCTATGTGCACAATGGATATTAAATATTGTAAACCAG
GTGAAAAACCCTTTGGTTGTTGCCCATGTCGTGAAAATTTAACTCAATAAAAAATTAATA
AAATATATATA----------
Length of 5' end seq. 671
3' end seq. ID SLE532Z
3' end seq. 
>SLE532Z.Seq
----------CCGGTTGTTGTCCATGTACTCTCCCACCAACCAAACCAGATTGCTCCCTG
GTTAAATGTGCTAGACCAGTATGTAAACCATATTATAGATTAAACATGACCGATTCTTGT
TGTGGTCGTTGTGAACCATGTACAGGTGTCGCATGTACTCTTCAAATGAAATATTGTAAA
GATGGTGAAGTTCCAACTGGTTGTTGTCCATGTACACCTCAACCAACTAAAAAACCAGAT
TGTTCAAAAGTTCCATGTCCAAAGATTTTAAAATATTGCCAAGAAGGTGAACTTCCAACT
GGTTGTTGTCCATGTACACCTCAACCAACTAAAAAACCAGATTGTTCAAGAGTTCCATGT
CCAAAGATTGTGAAATACTGTAAAGAAGGTGAACTTCCAACTGGTTGTTGTCCATGTACA
CCTCAACCAACTAAAAAACCAGATTGTTCTGATGTAATGTGTACAATGGACATTAGATAT
TGTAAAAATGGTGAACTTCCAACTGGTTGTTGTCCTTGCACACCTCAAGAAACCAAAGTT
CCAGATTGTTCTAAAGCTATGTGCACAATGGATATTAAATATTGTAAACCAGGTGAAAAA
CCCTTTGGTTGTTGCCCATGTCGTGAAAATTTAACTCAATAAAAAATTAATAAAATATAT
ATATTTGTTTTTTTAATT
Length of 3' end seq. 668
Connected seq. ID SLE532P
Connected seq. 
>SLE532P.Seq
AAGATGGTGAAGTTCCAACCGGTTGTTGTCCATGTACTCTCCCACCAACCAAACCAGATT
GCTCCCTTGTTAAATGTGCTAGACCAGTATGTAAACCATATTATAGATTAAACATGACCG
ATTCTTGTTGTGGTCGTTGTGAACCATGTACAGGTGTCGCATGTACTCTTCAAATCAAAT
ATTGTAAAGATGGTGAAGTTCCAACTGGTTGTTGTCCATGTACACCTCAACCAACTAAAA
AACCAGATTGTTCAAAAGTTCCATGTCCAAAGATTTTAAAATATTGCCAAGAAGGTGAAC
TTCCAACTGGTTGTTGTCCATGTACACCTCAACCAACTAAAAAACCAGATTGTTCAAGAG
TTCCATGTCCAAAGATTTTGAAATACTGTAAAGAAGGTGAACTTCCAACTGGTTGTTGTC
CATGTACACCTCAACCAACTAAAAAACCAGATTGTTCTGATGTAATGTGTACAATGGACA
TTAGATATTGTAAAAATGGTGAACTTCCAACTGGTTGTTGTCCTTGCACACCTCAAGAAA
CCAAAGTTCCAGATTGTTCTAAAGCTATGTGCACAATGGATATTAAATATTGTAAACCAG
GTGAAAAACCCTTTGGTTGTTGCCCATGTCGTGAAAATTTAACTCAATAAAAAATTAATA
AAATATATATA----------CCGGTTGTTGTCCATGTACTCTCCCACCAACCAAACCAG
ATTGCTCCCTGGTTAAATGTGCTAGACCAGTATGTAAACCATATTATAGATTAAACATGA
CCGATTCTTGTTGTGGTCGTTGTGAACCATGTACAGGTGTCGCATGTACTCTTCAAATGA
AATATTGTAAAGATGGTGAAGTTCCAACTGGTTGTTGTCCATGTACACCTCAACCAACTA
AAAAACCAGATTGTTCAAAAGTTCCATGTCCAAAGATTTTAAAATATTGCCAAGAAGGTG
AACTTCCAACTGGTTGTTGTCCATGTACACCTCAACCAACTAAAAAACCAGATTGTTCAA
GAGTTCCATGTCCAAAGATTGTGAAATACTGTAAAGAAGGTGAACTTCCAACTGGTTGTT
GTCCATGTACACCTCAACCAACTAAAAAACCAGATTGTTCTGATGTAATGTGTACAATGG
ACATTAGATATTGTAAAAATGGTGAACTTCCAACTGGTTGTTGTCCTTGCACACCTCAAG
AAACCAAAGTTCCAGATTGTTCTAAAGCTATGTGCACAATGGATATTAAATATTGTAAAC
CAGGTGAAAAACCCTTTGGTTGTTGCCCATGTCGTGAAAATTTAACTCAATAAAAAATTA
ATAAAATATATATATTTGTTTTTTTAATT
Length of connected seq. 1339
Full length Seq ID SLE532E
Full length Seq. 
>SLE532E.Seq
AAGATGGTGAAGTTCCAACCGGTTGTTGTCCATGTACTCTCCCACCAACCAAACCAGATT
GCTCCCTNGTTAAATGTGCTAGACCAGTATGTAAACCATATTATAGATTAAACATGACCG
ATTCTTGTTGTGGTCGTTGTGAACCATGTACAGGTGTCGCATGTACTCTTCAAATNAAAT
ATTGTAAAGATGGTGAAGTTCCAACTGGTTGTTGTCCATGTACACCTCAACCAACTAAAA
AACCAGATTGTTCAAAAGTTCCATGTCCAAAGATTTTAAAATATTGCCAAGAAGGTGAAC
TTCCAACTGGTTGTTGTCCATGTACACCTCAACCAACTAAAAAACCAGATTGTTCAAGAG
TTCCATGTCCAAAGATTNTGAAATACTGTAAAGAAGGTGAACTTCCAACTGGTTGTTGTC
CATGTACACCTCAACCAACTAAAAAACCAGATTGTTCTGATGTAATGTGTACAATGGACA
TTAGATATTGTAAAAATGGTGAACTTCCAACTGGTTGTTGTCCTTGCACACCTCAAGAAA
CCAAAGTTCCAGATTGTTCTAAAGCTATGTGCACAATGGATATTAAATATTGTAAACCAG
GTGAAAAACCCTTTGGTTGTTGCCCATGTCGTGAAAATTTAACTCAATAAAAAATTAATA
AAATATATATATTTGTTTTTTTAATT
Length of full length seq. 686