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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLE518
Library SL
(Link to library)
Clone ID SLE518
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16430-1
Original site URL
Representative seq. ID SLE518Z
(Link to Original site)
Representative DNA sequence 
>SLE518 (SLE518Q) /CSM/SL/SLE5-A/SLE518Q.Seq.d/
XXXXXXXXXXCAATTACCAAAAGAAGGTTTGAAATTAGATGAAACCGAAGATGAAAAGAA
AAAAGCTGAACAAGATAAAGCTGCCAATGAAGAATTACTCAAACAAGTTAAAGATGTATT
GGGTGATAAAGTTGAAAAGGTAGTTCTCTCAACTCGTCTTGCCAACTCACCATGTGTTTT
AGTTACCTCTGAATATGGTTGGTCAGCAAACATGGAACGTATCATGAAAGCTCAAGCTCT
CCGTGACTCTTCAATGTCATCATACATGTCATCAAAGAAAACTTTAGAATTAAATCCAGA
TCACCCAATCGTTAGAGATTTAGCCAAAAAAGCCGCCGAAAAAAGTAAAACTTTCAAAGA
CTTTGTTTACTTATTATATGAAACTGCTCTCTTAACTTCAGGTTTCTCATTAGATGAACC
ATCATCATTTGCTTCACGTATTCATCGTATGATTAAATTAGGTTTAAGCATTCAAGATGA
TTCATCAGCAACAACTGAAGAATCAACAAACACAACAACCAGTGATGATATTCCACCATT
AGAAGAAAACGATGAACCATCTGAAATGGAAAAAGTCGACTAGATTTAATTACTAGCCCT
TTTGCTATCAATTTTTTGGAATATTGTTATATTATTATAATAAAATAAATTTTAAAAAAA
ATCTTCA
sequence update 1998. 5.24
Translated Amino Acid sequence 
---LPKEGLKLDETEDEKKKAEQDKAANEELLKQVKDVLGDKVEKVVLSTRLANSPCVLV
TSEYGWSANMERIMKAQALRDSSMSSYMSSKKTLELNPDHPIVRDLAKKAAEKSKTFKDF
VYLLYETALLTSGFSLDEPSSFASRIHRMIKLGLSIQDDSSATTEESTNTTTSDDIPPLE
ENDEPSEMEKVD*i*llalllsifwnivill**nkf*kkss
Translated Amino Acid sequence (All Frames) 
Frame A:
---nyqkkv*n*mkpkmkrkklnkiklpmknysnklkmywviklkr*fsqlvlpthhvf*
lplnmvgqqtwnvs*klklsvtlqchhtchqrkl*n*iqitqslei*pkkppkkvklskt
lftyymklls*lqvsh*mnhhhllhvfiv*ln*v*afkmihqqqlknqqtqqpvmifhh*
kktmnhlkwkkstrfny*pfcyqffgillyyynkinfkknl


Frame B:
---itkrrfeir*nrr*keks*tr*scq*ritqts*rcig**s*kgsslnsscqltmcfs
yl*iwlvskhgtyhessssp*lfnviihvikenfriksrspnr*rfsqksrrkk*nfqrl
cllii*ncslnfrflir*tiiicftyssyd*irfkhsr*fisnn*rinkhnnq**ystir
rkr*ti*ngksrldlitspfainfleycyiiiik*ilkkif


Frame C:
---LPKEGLKLDETEDEKKKAEQDKAANEELLKQVKDVLGDKVEKVVLSTRLANSPCVLV
TSEYGWSANMERIMKAQALRDSSMSSYMSSKKTLELNPDHPIVRDLAKKAAEKSKTFKDF
VYLLYETALLTSGFSLDEPSSFASRIHRMIKLGLSIQDDSSATTEESTNTTTSDDIPPLE
ENDEPSEMEKVD*i*llalllsifwnivill**nkf*kkss
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLE518 (SLE518Q) /CSM/SL/SLE5-A/SLE518Q.Seq.d/                        624   e-178
VHQ442 (VHQ442Q) /CSM/VH/VHQ4-B/VHQ442Q.Seq.d/                        609   e-173
VHQ333 (VHQ333Q) /CSM/VH/VHQ3-B/VHQ333Q.Seq.d/                        609   e-173
VHP779 (VHP779Q) /CSM/VH/VHP7-D/VHP779Q.Seq.d/                        609   e-173
VHP202 (VHP202Q) /CSM/VH/VHP2-A/VHP202Q.Seq.d/                        609   e-173
VHP148 (VHP148Q) /CSM/VH/VHP1-B/VHP148Q.Seq.d/                        609   e-173
VHO658 (VHO658Q) /CSM/VH/VHO6-C/VHO658Q.Seq.d/                        609   e-173
VHN866 (VHN866Q) /CSM/VH/VHN8-C/VHN866Q.Seq.d/                        609   e-173
VHN670 (VHN670Q) /CSM/VH/VHN6-C/VHN670Q.Seq.d/                        609   e-173
VHN633 (VHN633Q) /CSM/VH/VHN6-B/VHN633Q.Seq.d/                        609   e-173
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU035029) Dictyostelium discoideum slug cDNA, clone SLE518.      624   0.0    2
(BJ446614) Dictyostelium discoideum cDNA clone:ddv63d12, 3' ...   607   0.0    4
(BJ446603) Dictyostelium discoideum cDNA clone:ddv63b09, 3' ...   607   0.0    2
(BJ446503) Dictyostelium discoideum cDNA clone:ddv62n19, 3' ...   607   0.0    2
(BJ446041) Dictyostelium discoideum cDNA clone:ddv61o11, 3' ...   607   0.0    3
(BJ445907) Dictyostelium discoideum cDNA clone:ddv61c02, 3' ...   607   0.0    2
(BJ445748) Dictyostelium discoideum cDNA clone:ddv60c16, 3' ...   607   0.0    2
(BJ445166) Dictyostelium discoideum cDNA clone:ddv58k18, 3' ...   607   0.0    4
(BJ445137) Dictyostelium discoideum cDNA clone:ddv58e17, 3' ...   607   0.0    2
(BJ445133) Dictyostelium discoideum cDNA clone:ddv58d18, 3' ...   607   0.0    4
dna update 2009. 4. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54651) RecName: Full=Heat shock cognate 90 kDa protein;             254   1e-66
L43591_1(L43591|pid:none) Dictyostelium discoideum (clone C 6.1....   254   1e-66
DQ884433_1(DQ884433|pid:none) Karlodinium micrum clone Kmi-5p-cD...   176   4e-43
AB267894_1(AB267894|pid:none) Prymnesium parvum mRNA for heat sh...   174   2e-42
DQ062086_1(DQ062086|pid:none) Pseudourostyla cristata heat shock...   174   3e-42
AB092411_1(AB092411|pid:none) Entamoeba histolytica gene for cyt...   174   3e-42
AM423851_1(AM423851|pid:none) Vitis vinifera contig VV78X192584....   172   6e-42
AY292370_1(AY292370|pid:none) Toxoplasma gondii HSP90 mRNA, comp...   172   6e-42
AY831450_1(AY831450|pid:none) Monocercomonoides sp. PA203 heat s...   171   1e-41
AM426751_2(AM426751|pid:none) Vitis vinifera contig VV78X138759....   171   2e-41
protein update 2009. 6. 3
PSORT 

 psg: 0.75  gvh: 0.37  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for SLE518 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID SLE518Z
3' end seq. 
>SLE518Z.Seq
----------CAATTACCAAAAGAAGGTTTGAAATTAGATGAAACCGAAGATGAAAAGAA
AAAAGCTGAACAAGATAAAGCTGCCAATGAAGAATTACTCAAACAAGTTAAAGATGTATT
GGGTGATAAAGTTGAAAAGGTAGTTCTCTCAACTCGTCTTGCCAACTCACCATGTGTTTT
AGTTACCTCTGAATATGGTTGGTCAGCAAACATGGAACGTATCATGAAAGCTCAAGCTCT
CCGTGACTCTTCAATGTCATCATACATGTCATCAAAGAAAACTTTAGAATTAAATCCAGA
TCACCCAATCGTTAGAGATTTAGCCAAAAAAGCCGCCGAAAAAAGTAAAACTTTCAAAGA
CTTTGTTTACTTATTATATGAAACTGCTCTCTTAACTTCAGGTTTCTCATTAGATGAACC
ATCATCATTTGCTTCACGTATTCATCGTATGATTAAATTAGGTTTAAGCATTCAAGATGA
TTCATCAGCAACAACTGAAGAATCAACAAACACAACAACCAGTGATGATATTCCACCATT
AGAAGAAAACGATGAACCATCTGAAATGGAAAAAGTCGACTAGATTTAATTACTAGCCCT
TTTGCTATCAATTTTTTGGAATATTGTTATATTATTATAATAAAATAAATTTTAAAAAAA
ATCTTCA
Length of 3' end seq. 657
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -