>SLE502 (SLE502Q) /CSM/SL/SLE5-A/SLE502Q.Seq.d/
GGATGGTGAAGATGTTCAAGCTTTAGTTATTGATAACGGTTCTGGTATGTGTAAAGCCGG
TTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACA
CACTGGTGTTATGGTCGGTATGGGTCAAAAGGATTCATATGTAGGTGATGAAGCTCAATC
AAAAAGAGGTATCTTAACTCTCAAATATCCAATTGAACATGGAATTGTCACCAATTGGGA
TGATATGGAAAAGATCTGGCATCATACTTTCTACAATGAACTTCGTGTTGCACCAGAAGA
ACATCCAGTTCTCTTAACCGAAGCACCATTAAATCCAAAAGCAAACAGAGAAAAGATGAC
TCAAATTATGTTTGAAACTTTCAACACTCCAGCTATGTACGTTGCCATTCAAGCTGTCTT
ATCATTATATGCCTCTGGTCGTACCACTGGTATCGTTATGGATTCAGGTGATGGTGTCTC
CCACACTGTACCAATCTATGAAGGTTATGCTTTACCACATGCCATCTTACGTTTAGATTT
AGCAGGTCGTGATCTTACTGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATT
CACCACCACCGCCGAAAGAGAAATTGTCAGAGATATCAAAGAAAAATTAGCATACGTCGC
CXXXXXXXXXX

DGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDSYVGDEAQS
KRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT
QIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVSHTVPIYEGYALPHAILRLDL
AGRDLTDYMMKILTERGYSFTTTAEREIVRDIKEKLAYV---

Frame A:
gw*rcssfsy**rfwyv*srfcw**cstccfpincwsskthwcygrygskgficr**ssi
kkrylnsqisn*twnchqlg*ygkdlasyflq*tscctrrtssslnrstikskskqrkdd
snyv*nfqhssyvrchsscliiiclwsyhwyrygfr*wclphctnl*rlcfttchltfrf
srs*sy*lhdenln*twllihhhrrkrncqryqrkisirr---


Frame B:
DGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDSYVGDEAQS
KRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT
QIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVSHTVPIYEGYALPHAILRLDL
AGRDLTDYMMKILTERGYSFTTTAEREIVRDIKEKLAYV---


Frame C:
mvkmfkl*llitvlvcvkpvllvmmlhvlfshqllvvqdtlvlwsvwvkrihm*vmklnq
kevs*lsniqlnmelspigmiwkrsgiilstmnfvlhqkniqfs*pkhh*iqkqtekr*l
klclklstlqlctlpfklsyhymplvvplvslwiqvmvsptlyqsmkvmlyhmpsyv*i*
qvvillit**ks*lnvvthsppppkeklseiskkn*hts---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL865 (VFL865Q) /CSM/VF/VFL8-C/VFL865Q.Seq.d/                       1310   0.0
SLE502 (SLE502Q) /CSM/SL/SLE5-A/SLE502Q.Seq.d/                       1310   0.0
VFB424 (VFB424Q) /CSM/VF/VFB4-A/VFB424Q.Seq.d/                       1304   0.0
VFK236 (VFK236Q) /CSM/VF/VFK2-B/VFK236Q.Seq.d/                       1302   0.0
FC-AH11 (FC-AH11Q) /CSM/FC/FC-AH/FC-AH11Q.Seq.d/                     1302   0.0
SLE248 (SLE248Q) /CSM/SL/SLE2-B/SLE248Q.Seq.d/                       1211   0.0
VFN368 (VFN368Q) /CSM/VF/VFN3-C/VFN368Q.Seq.d/                       1203   0.0
VFE476 (VFE476Q) /CSM/VF/VFE4-D/VFE476Q.Seq.d/                       1172   0.0
VFM796 (VFM796Q) /CSM/VF/VFM7-D/VFM796Q.Seq.d/                       1156   0.0
VFK304 (VFK304Q) /CSM/VF/VFK3-A/VFK304Q.Seq.d/                       1148   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC116956) Dictyostelium discoideum chromosome 2 map 1418423...  1310   0.0    1
(AU061510) Dictyostelium discoideum slug cDNA, clone SLE502.     1310   0.0    1
(AU284282) Dictyostelium discoideum gamete cDNA clone:FC-AH1...  1302   0.0    1
(X03282) dictyostelium discoideum gene for actin A12.            1263   0.0    1
(AU061436) Dictyostelium discoideum slug cDNA, clone SLE248.     1211   0.0    1
(BJ416870) Dictyostelium discoideum cDNA clone:ddv27h17, 5' ...  1203   0.0    1
(BJ415068) Dictyostelium discoideum cDNA clone:ddv21g10, 5' ...  1201   0.0    1
(BJ411156) Dictyostelium discoideum cDNA clone:ddv3p06, 5' e...  1187   0.0    1
(BJ412783) Dictyostelium discoideum cDNA clone:ddv9h20, 5' e...  1172   0.0    1
(BJ416613) Dictyostelium discoideum cDNA clone:ddv26p23, 5' ...  1156   0.0    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   447   e-124
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   447   e-124
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   447   e-124
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   444   e-124
AY294152_1(AY294152|pid:none) Mayorella sp. JJP-2003 actin mRNA,...   441   e-123
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        441   e-123
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   439   e-122
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   437   e-121
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   437   e-121
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          437   e-121

 psg: 0.75  gvh: 0.34  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.07  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SLE502 is cyt

>SLE502F.Seq
GGATGGTGAAGATGTTCAAGCTTTAGTTATTGATAACGGTTCTGGTATGTGTAAAGCCGG
TTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACA
CACTGGTGTTATGGTCGGTATGGGTCAAAAGGATTCATATGTAGGTGATGAAGCTCAATC
AAAAAGAGGTATCTTAACTCTCAAATATCCAATTGAACATGGAATTGTCACCAATTGGGA
TGATATGGAAAAGATCTGGCATCATACTTTCTACAATGAACTTCGTGTTGCACCAGAAGA
ACATCCAGTTCTCTTAACCGAAGCACCATTAAATCCAAAAGCAAACAGAGAAAAGATGAC
TCAAATTATGTTTGAAACTTTCAACACTCCAGCTATGTACGTTGCCATTCAAGCTGTCTT
ATCATTATATGCCTCTGGTCGTACCACTGGTATCGTTATGGATTCAGGTGATGGTGTCTC
CCACACTGTACCAATCTATGAAGGTTATGCTTTACCACATGCCATCTTACGTTTAGATTT
AGCAGGTCGTGATCTTACTGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATT
CACCACCACCGCCGAAAGAGAAATTGTCAGAGATATCAAAGAAAAATTAGCATACGTCGC
C----------