>SLE427 (SLE427Q) /CSM/SL/SLE4-B/SLE427Q.Seq.d/
XXXXXXXXXXACTCATTCACCACCACTGCCGAAAGAGAAATCGTCAGAGATATCAAAGAG
AAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCCTCATCATCA
GCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTTATCACCATTGGTAACGAACGT
TTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCTGCTGGTATC
CACGAAACCACATACAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTTATAC
GGTAATGTCGTCTTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAAC
AAAGAATTAACTGCTTTAGCACCATCAACCATGAAAATCAAAATCATTGCTCCACCAGAA
CGTAAATACTCTGTCTGGATTGGTGGATCTATTTTAGCTTCACTCTCAACTTTCCAACAA
ATGTGGATCTCAAAAGAAGAATATGATGAATCTGGTCCATCAATTGTCCACAGAAAATGT
TTCTAAATCATGATGAAAGTGCGTCAGNNNNNNNNAATAATAATAATATAACAATAATAA
TATTTAAATGTATAATAAAATTTAATTACTTTTTTTTTAATGGTTGTTGATCTTTATCCG
ACCTTAAAAAAAAAAAAATAAAACCAAA

---SFTTTAEREIVRDIKEKLAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERF
RCPEALFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNK
ELTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIVHRKCF
*immkvrqxxxiiii*q**ylnv**nlitfflmvvdlyptlkkkk*nq

Frame A:
---lihhhcrkrnrqryqreislrrprl*s*nanccliisprkiiritrrssyhhw*rtf
plprsplptiilrygicwyprnhiqlhhem*c*yp*rfir*crlirwynyvpryc*syeq
rincfstinhenqnhcstrt*ilcldwwiyfsftlnfptnvdlkrri**iwsincpqkmf
lnhdesasxxxnnnnitiiifkciikfnyfffngc*slsdlkkkkikp


Frame B:
---SFTTTAEREIVRDIKEKLAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERF
RCPEALFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNK
ELTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIVHRKCF
*immkvrqxxxiiii*q**ylnv**nlitfflmvvdlyptlkkkk*nq


Frame C:
---hspplpkeksseiskrn*ptspstlklkckllphhqpsknhtnyqtvklsplvtnvs
vaqkpssnhhs*vwnllvstkphttps*nvmlisvkiytvmssyqvvqlcsqvlliv*tk
n*ll*hhqp*ksksllhqnvntlsglvdlf*lhsqlsnkcgsqkknmmnlvhqlstenvs
ks**kcvxxxx***ynnnni*mynki*llff*wllifirp*kkknktk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLE427 (SLE427Q) /CSM/SL/SLE4-B/SLE427Q.Seq.d/                       1104   0.0
SSK713 (SSK713Q) /CSM/SS/SSK7-A/SSK713Q.Seq.d/                       1078   0.0
SLH825 (SLH825Q) /CSM/SL/SLH8-B/SLH825Q.Seq.d/                       1078   0.0
SLH433 (SLH433Q) /CSM/SL/SLH4-B/SLH433Q.Seq.d/                       1078   0.0
SLA782 (SLA782Q) /CSM/SL/SLA7-D/SLA782Q.Seq.d/                       1078   0.0
SLA636 (SLA636Q) /CSM/SL/SLA6-B/SLA636Q.Seq.d/                       1078   0.0
SFI391 (SFI391Q) /CSM/SF/SFI3-D/SFI391Q.Seq.d/                       1078   0.0
AFM462 (AFM462Q) /CSM/AF/AFM4-C/AFM462Q.Seq.d/                       1078   0.0
AFM274 (AFM274Q) /CSM/AF/AFM2-D/AFM274Q.Seq.d/                       1078   0.0
SLE658 (SLE658Q) /CSM/SL/SLE6-C/SLE658Q.Seq.d/                       1072   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115680|AC115680.3 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.  1078   0.0    3
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.        1078   0.0    3
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   946   0.0    9
X03281|X03281.1 Dictyostelium discoideum gene for actin A8.       944   0.0    1
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   874   0.0    2
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   837   0.0    9
X03282|X03282.1 dictyostelium discoideum gene for actin A12.      817   0.0    1
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   813   0.0    2
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   700   0.0    1
AX771242|AX771242.1 Sequence 5 from Patent WO03034275.            547   e-172  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   354   2e-96
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   354   2e-96
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        354   2e-96
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   350   3e-95
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   350   3e-95
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   350   3e-95
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          347   2e-94
AF508263_1(AF508263|pid:none) Pyrocystis lunula actin mRNA, comp...   347   2e-94
AY615868_1(AY615868|pid:none) Amastigomonas marina actin gene, p...   346   4e-94
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   344   1e-93

 psg: 0.75  gvh: 0.38  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 20.0 %: cytoplasmic
	 20.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for SLE427 is nuc

>SLE427Z.Seq
----------ACTCATTCACCACCACTGCCGAAAGAGAAATCGTCAGAGATATCAAAGAG
AAATTAGCCTACGTCGCCCTCGACTTTGAAGCTGAAATGCAAACTGCTGCCTCATCATCA
GCCCTCGAAAAATCATACGAATTACCAGACGGTCAAGTTATCACCATTGGTAACGAACGT
TTCCGTTGCCCAGAAGCCCTCTTCCAACCATCATTCTTAGGTATGGAATCTGCTGGTATC
CACGAAACCACATACAACTCCATCATGAAATGTGATGTTGATATCCGTAAAGATTTATAC
GGTAATGTCGTCTTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAAC
AAAGAATTAACTGCTTTAGCACCATCAACCATGAAAATCAAAATCATTGCTCCACCAGAA
CGTAAATACTCTGTCTGGATTGGTGGATCTATTTTAGCTTCACTCTCAACTTTCCAACAA
ATGTGGATCTCAAAAGAAGAATATGATGAATCTGGTCCATCAATTGTCCACAGAAAATGT
TTCTAAATCATGATGAAAGTGCGTCAGNNNNNNNNAATAATAATAATATAACAATAATAA
TATTTAAATGTATAATAAAATTTAATTACTTTTTTTTTAATGGTTGTTGATCTTTATCCG
ACCTTAAAAAAAAAAAAATAAAACCAAA