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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFB248
Library AF
(Link to library)
Clone ID AFB248
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16294-1
Original site URL
Representative seq. ID AFB248P
(Link to Original site)
Representative DNA sequence 
>AFB248 (AFB248Q) /CSM/AF/AFB2-B/AFB248Q.Seq.d/
AAAATTAAATTAAATAAAATGTCATTAAATATTGAAAAAGTTAAAGAATTTAGTAATAAA
GCATGGGATGAAAATATTTTACCAACACTTTCAAAATATATTGAAATTCCAAATCAATCA
CCATTATATGATGCAGAATGGGCAACCAATGGTTATACAGAAGAAGCAATTCAATTATTA
GAGAAATGGATCTTAGCACAAGGTATCAAAGGTATTTCATGTGAAATTAAAAGAATTGAA
GGATTAACACCAATTATTTTCATGGTAGTCGAAGCAACCAAAACTGAAAAAGTTAAAACT
GTACTTTTATATGGTCACATGGATAAACAACCACCACTCACTGATGCATGGGATGAAGGA
TTACATCCATACAAAGCAGTCATCAAGAATGACAAACTCTATGGTCGTGGTGGTGCAGAT
GATGGTTATTCAACATTTGCTTCAATCTCTGCAATCAAAGCATTGCAAGAACAATCAATT
CCACATGATCGTTATXXXXXXXXXXGAAGGTGGTTCCATCCCATTCATGAAAATGTTACA
TGACCGTTTCAATTGTCAATTTATCGTTACTGGTTTATTAGGTCCAGGTTCAAATGCTCA
TGGTCCAAATGAATTCTTAGATATTCCATTTGCCAAGAAATTATTAAGTTGTGTTATTTA
TTTATTAGATGTCTCNTGCCAAGTTTG
sequence update 2001. 6. 1
Translated Amino Acid sequence 
KIKLNKMSLNIEKVKEFSNKAWDENILPTLSKYIEIPNQSPLYDAEWATNGYTEEAIQLL
EKWILAQGIKGISCEIKRIEGLTPIIFMVVEATKTEKVKTVLLYGHMDKQPPLTDAWDEG
LHPYKAVIKNDKLYGRGGADDGYSTFASISAIKALQEQSIPHDRY---

---EGGSIPFMKMLHDRFNCQFIVTGLLGPGSNAHGPNEFLDIPFAKKLLSCVIYLLDVS
CQV
Translated Amino Acid sequence (All Frames) 
Frame A:
KIKLNKMSLNIEKVKEFSNKAWDENILPTLSKYIEIPNQSPLYDAEWATNGYTEEAIQLL
EKWILAQGIKGISCEIKRIEGLTPIIFMVVEATKTEKVKTVLLYGHMDKQPPLTDAWDEG
LHPYKAVIKNDKLYGRGGADDGYSTFASISAIKALQEQSIPHDRY---

---EGGSIPFMKMLHDRFNCQFIVTGLLGPGSNAHGPNEFLDIPFAKKLLSCVIYLLDVS
CQV

Frame B:
kln*ikch*ilkklknlvikhgmkifyqhfqnilkfqinhhymmqngqpmviqkkqfny*
rngs*hkvskvfhvklkelkd*hqlfsw*skqpklkklklyfymvtwinnhhslmhgmkd
yihtkqssrmtnsmvvvvqmmviqhllqslqskhcknnqfhmiv---

---kvvpshs*kcymtvsivnlsllvy*vqvqmlmvqmns*ifhlprny*vvlfiy*msx
akf

Frame C:
n*ik*nviky*ks*ri***smg*kyftntfkiy*nsksitii*crmgnqwlyrrsnsiir
emdlstryqryfm*n*kn*rintnyfhgsrsnqn*ks*nctfiwshg*tttth*cmg*ri
tsiqsshqe*qtlwswwcr*wlfnicfnlcnqsiartinst*sl---

---rwfhpihenvt*pfqlsiyrywfirsrfkcswsk*ilrysicqeiiklcylfirclx
psl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFB248 (AFB248Q) /CSM/AF/AFB2-B/AFB248Q.Seq.d/                       1178   0.0
VSK267 (VSK267Q) /CSM/VS/VSK2-C/VSK267Q.Seq.d/                        854   0.0
VFO152 (VFO152Q) /CSM/VF/VFO1-C/VFO152Q.Seq.d/                        854   0.0
VFN844 (VFN844Q) /CSM/VF/VFN8-B/VFN844Q.Seq.d/                        854   0.0
VFN570 (VFN570Q) /CSM/VF/VFN5-C/VFN570Q.Seq.d/                        854   0.0
VFN247 (VFN247Q) /CSM/VF/VFN2-B/VFN247Q.Seq.d/                        854   0.0
VFK772 (VFK772Q) /CSM/VF/VFK7-C/VFK772Q.Seq.d/                        854   0.0
VFK163 (VFK163Q) /CSM/VF/VFK1-C/VFK163Q.Seq.d/                        854   0.0
VFI895 (VFI895Q) /CSM/VF/VFI8-D/VFI895Q.Seq.d/                        854   0.0
VFC720 (VFC720Q) /CSM/VF/VFC7-A/VFC720Q.Seq.d/                        854   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC092544|AC092544.4 Homo sapiens BAC clone RP11-317F4 from 4, complete sequence.    38  0.089  5
CD821342|CD821342.1 BN25.041I21F011127 BN25 Brassica napus cDNA clone BN25041I21, mRNA sequence.    38  0.14  2
BI433279|BI433279.1 EST536040 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCBC62 5' sequence, mRNA sequence.    48  0.14  1
BQ046631|BQ046631.1 EST595749 P. infestans-challenged potato leaf, incompatible reaction Solanum tuberosum cDNA clone BPLI15C18 5' end, mRNA sequence.    48  0.14  1
BI435717|BI435717.1 EST538478 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCCI86 5' sequence, mRNA sequence.    48  0.14  1
BI435853|BI435853.1 EST538614 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCCL22 5' sequence, mRNA sequence.    48  0.14  1
BG591390|BG591390.1 EST499232 P. infestans-challenged leaf Solanum tuberosum cDNA clone BPLI8D17 5' sequence, mRNA sequence.    48  0.14  1
BQ112173|BQ112173.1 EST597749 mixed potato tissues Solanum tuberosum cDNA clone STMCF59 3' end, mRNA sequence.    48  0.14  1
BI435340|BI435340.1 EST538101 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCCD08 5' sequence, mRNA sequence.    48  0.14  1
CC535979|CC535979.1 CH240_414D20.TARBAC13P2 CHORI-240 Bos taurus genomic clone CH240_414D20, genomic survey sequence.    36  0.16  2
dna update 2003. 7.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ384330_1(DQ384330|pid:none) Hartmannella vermiformis succinyl-...   186   5e-46
DQ384331_1(DQ384331|pid:none) Physarum polycephalum succinyl-dia...   177   3e-43
CP001111_978(CP001111|pid:none) Stenotrophomonas maltophilia R55...   172   8e-42
AM743169_1072(AM743169|pid:none) Stenotrophomonas maltophilia K2...   172   8e-42
AE008923_2905(AE008923|pid:none) Xanthomonas axonopodis pv. citr...   169   7e-41
AM502249_240(AM502249|pid:none) Leishmania infantum chromosome 31.    167   3e-40
CT005268_204(CT005268|pid:none) Leishmania major strain Friedlin...   164   2e-39
CT005270_197(CT005270|pid:none) Leishmania major strain Friedlin...   162   1e-38
CP000113_526(CP000113|pid:none) Myxococcus xanthus DK 1622, comp...   160   2e-38
AM502249_238(AM502249|pid:none) Leishmania infantum chromosome 31.    159   9e-38
protein update 2009. 3.26
PSORT 

 psg: 0.66  gvh: 0.29  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 40.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for AFB248 is nuc
5' end seq. ID AFB248F
5' end seq. 
>AFB248F.Seq
AAAATTAAATTAAATAAAATGTCATTAAATATTGAAAAAGTTAAAGAATTTAGTAATAAA
GCATGGGATGAAAATATTTTACCAACACTTTCAAAATATATTGAAATTCCAAATCAATCA
CCATTATATGATGCAGAATGGGCAACCAATGGTTATACAGAAGAAGCAATTCAATTATTA
GAGAAATGGATCTTAGCACAAGGTATCAAAGGTATTTCATGTGAAATTAAAAGAATTGAA
GGATTAACACCAATTATTTTCATGGTAGTCGAAGCAACCAAAACTGAAAAAGTTAAAACT
GTACTTTTATATGGTCACATGGATAAACAACCACCACTCACTGATGCATGGGATGAAGGA
TTACATCCATACAAAGCAGTCATCAAGAATGACAAACTCTATGGTCGTGGTGGTGCAGAT
GATGGTTATTCAACATTTGCTTCAATCTCTGCAATCAAAGCATTGCAAGAACAATCAATT
CCACATGATCGTTAT----------
Length of 5' end seq. 495
3' end seq. ID AFB248Z
3' end seq. 
>AFB248Z.Seq
----------GAAGGTGGTTCCATCCCATTCATGAAAATGTTACATGACCGTTTCAATTG
TCAATTTATCGTTACTGGTTTATTAGGTCCAGGTTCAAATGCTCATGGTCCAAATGAATT
CTTAGATATTCCATTTGCCAAGAAATTATTAAGTTGTGTTATTTATTTATTAGATGTCTC
NTGCCAAGTTTG
Length of 3' end seq. 182
Connected seq. ID AFB248P
Connected seq. 
>AFB248P.Seq
AAAATTAAATTAAATAAAATGTCATTAAATATTGAAAAAGTTAAAGAATTTAGTAATAAA
GCATGGGATGAAAATATTTTACCAACACTTTCAAAATATATTGAAATTCCAAATCAATCA
CCATTATATGATGCAGAATGGGCAACCAATGGTTATACAGAAGAAGCAATTCAATTATTA
GAGAAATGGATCTTAGCACAAGGTATCAAAGGTATTTCATGTGAAATTAAAAGAATTGAA
GGATTAACACCAATTATTTTCATGGTAGTCGAAGCAACCAAAACTGAAAAAGTTAAAACT
GTACTTTTATATGGTCACATGGATAAACAACCACCACTCACTGATGCATGGGATGAAGGA
TTACATCCATACAAAGCAGTCATCAAGAATGACAAACTCTATGGTCGTGGTGGTGCAGAT
GATGGTTATTCAACATTTGCTTCAATCTCTGCAATCAAAGCATTGCAAGAACAATCAATT
CCACATGATCGTTAT----------GAAGGTGGTTCCATCCCATTCATGAAAATGTTACA
TGACCGTTTCAATTGTCAATTTATCGTTACTGGTTTATTAGGTCCAGGTTCAAATGCTCA
TGGTCCAAATGAATTCTTAGATATTCCATTTGCCAAGAAATTATTAAGTTGTGTTATTTA
TTTATTAGATGTCTCNTGCCAAGTTTG
Length of connected seq. 677
Full length Seq ID -
Full length Seq. -
Length of full length seq. -