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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLE347
Library SL
(Link to library)
Clone ID SLE347
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1
Original site URL
Representative seq. ID SLE347Z
(Link to Original site)
Representative DNA sequence 
>SLE347 (SLE347Q) /CSM/SL/SLE3-B/SLE347Q.Seq.d/
XXXXXXXXXXAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCACCACAAGAAACT
GAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCCATGCTGGTTAC
TCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCGAT
AAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTA
AAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATNTCGTCCAATGTGTGTTGAATCC
TTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTCGCC
GTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTGATAAGAAAGGT
GCTGCTGCCCCATCAAAAAAGAAATAAATAAATAATATTTCTTAATAAATAGCATTTTAT
AAT
sequence update 1998. 5.22
Translated Amino Acid sequence 
---RGMVAGDSKNDPPQETEKFVAQVIVLNHPGQIHAGYSPVLDCHTAHIACKFTEIVDK
VDRRTGAVVAKEGTAAVVLKNGDAAMVELTPXRPMCVESFTEYPPLGRFAVRDMRQTVAV
GVIKSTVKKAPGKAGDKKGAAAPSKKK*innis**iafyn
Translated Amino Acid sequence (All Frames) 
Frame A:
---krygrw*lqkrsttrn*kircssyrpqpsrsnpcwlltslrlshcshcl*ih*nrr*
s*ssywcrrcqrrycrrrlkew*crygrinpxssnvc*ilh*iptirsfrrqryetnrrr
rchqinrqespr*sr**ercccpikkeink*yflinsil*


Frame B:
---RGMVAGDSKNDPPQETEKFVAQVIVLNHPGQIHAGYSPVLDCHTAHIACKFTEIVDK
VDRRTGAVVAKEGTAAVVLKNGDAAMVELTPXRPMCVESFTEYPPLGRFAVRDMRQTVAV
GVIKSTVKKAPGKAGDKKGAAAPSKKK*innis**iafyn


Frame C:
---evwslvtpktihhkklknsllklssstiqvksmlvthqs*ivtlltlpvnslkssik
livvlvpslpkkvlppss*rmvmplwsn*phxvqcvlnpslnthh*vvspsei*dkpsps
vssnqpsrkpqvkpvirkvllphqkrnk*iiflnk*hfi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSE380 (VSE380Q) /CSM/VS/VSE3-D/VSE380Q.Seq.d/                        932   0.0
VFN472 (VFN472Q) /CSM/VF/VFN4-C/VFN472Q.Seq.d/                        932   0.0
SLK447 (SLK447Q) /CSM/SL/SLK4-B/SLK447Q.Seq.d/                        932   0.0
SLH395 (SLH395Q) /CSM/SL/SLH3-D/SLH395Q.Seq.d/                        932   0.0
SLG782 (SLG782Q) /CSM/SL/SLG7-D/SLG782Q.Seq.d/                        932   0.0
SLG541 (SLG541Q) /CSM/SL/SLG5-B/SLG541Q.Seq.d/                        932   0.0
SLE701 (SLE701Q) /CSM/SL/SLE7-A/SLE701Q.Seq.d/                        932   0.0
SLE555 (SLE555Q) /CSM/SL/SLE5-C/SLE555Q.Seq.d/                        932   0.0
SLE403 (SLE403Q) /CSM/SL/SLE4-A/SLE403Q.Seq.d/                        932   0.0
SLE347 (SLE347Q) /CSM/SL/SLE3-B/SLE347Q.Seq.d/                        932   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU285021) Dictyostelium discoideum gamete cDNA clone:FCL-AA...   932   0.0    1
(AU284628) Dictyostelium discoideum gamete cDNA clone:FC-BF1...   932   0.0    1
(AU284342) Dictyostelium discoideum gamete cDNA clone:FC-AN0...   932   0.0    1
(AU284225) Dictyostelium discoideum gamete cDNA clone:FC-AA2...   932   0.0    1
(AU263066) Dictyostelium discoideum vegetative cDNA clone:VS...   932   0.0    1
(AU054027) Dictyostelium discoideum slug cDNA, clone SLK447.      932   0.0    1
(AU052352) Dictyostelium discoideum slug cDNA, clone SLD182.      932   0.0    1
(AU052273) Dictyostelium discoideum slug cDNA, clone SLA482.      932   0.0    1
(AU052271) Dictyostelium discoideum slug cDNA, clone SLA478.      932   0.0    1
(AU052263) Dictyostelium discoideum slug cDNA, clone SLA460.      932   0.0    1
dna update 2009. 3.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   249   2e-65
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   249   2e-65
(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   249   2e-65
AY643820_1(AY643820|pid:none) Arcyria denudata elongation factor...   186   2e-46
AJ581004_1(AJ581004|pid:none) Axinella verrucosa mRNA for elonga...   185   4e-46
AY643816_1(AY643816|pid:none) Lycogala epidendrum elongation fac...   184   9e-46
AY542532_1(AY542532|pid:none) Cladonema radiatum translation elo...   184   1e-45
(Q00251) RecName: Full=Elongation factor 1-alpha;          Short...   183   2e-45
AY582829_1(AY582829|pid:none) Acanthamoeba culbertsoni ef1a gene...   183   2e-45
(P40911) RecName: Full=Elongation factor 1-alpha;          Short...   183   2e-45
protein update 2009. 6. 3
PSORT 

 psg: 0.50  gvh: 0.50  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 24.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for SLE347 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID SLE347Z
3' end seq. 
>SLE347Z.Seq
----------AAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCACCACAAGAAACT
GAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCCATGCTGGTTAC
TCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCGAT
AAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTA
AAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATNTCGTCCAATGTGTGTTGAATCC
TTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTCGCC
GTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTGATAAGAAAGGT
GCTGCTGCCCCATCAAAAAAGAAATAAATAAATAATATTTCTTAATAAATAGCATTTTAT
AAT
Length of 3' end seq. 473
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -