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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLE328
Library SL
(Link to library)
Clone ID SLE328
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U16046-1
Original site URL
Representative seq. ID SLE328E
(Link to Original site)
Representative DNA sequence 
>SLE328 (SLE328Q) /CSM/SL/SLE3-B/SLE328Q.Seq.d/
ATTTTATTGCTTTTTTTAATTGTTTTCGTTGTTGCTCAAAGTTCATCATCATCATCCTCT
TCAGGATGTCCATGTGATTCATCATATTTATGTGAACCACTTCAAATTGCACCAAGACAA
GAGTTTTTAGGTTTTTCATTAAATAGCACGCAATATCCAAATTATTATTGGAATCAATTA
ACAACACTTGCGATATTTTATGAAACAATTGAAGATGAATTATTATGTATTGCACATGAA
AATGATGTTAGATTAGTATGGGGTACAACATTTCCAATTGAAAATCTTGGTAATTCAAGT
TATATTGAAGAATGGATTCAAGAACAAATTGAAAAAGTTCAAAGTACATTTACAGATGGT
TTAAATTTTGATGTTGAAAGTCCAATTACAGATCCAACCATCGCTCAACAATATACAGAA
TTAGTATCAGCAACTAATAAAGCATTTAAAGCAATTAATCCATTCTATCAAATTTCAATC
GATGTAGCATGGTCACCATCATGCATTNATAAACGTTGTTATGATTATGCTGGTTTAGCT
TCAAATTCCGATTTCCTTGTAGCAATGGACTATGATGAGAGAAGTCAAGTTTTTGGTGAG
AAGGTGTGTACTGCTGGTGCAAATTCTTCACCCTCAAATGCCTTGGCTGGTATAAATAAT
TTTACTGATTTAGGTATTAGCACTGATCAATTGGTAATGGGTTTACCATGGTATGGTTAT
ATTTATAAGAATTGTTTAAATGGTGATGAAGCAGGTTTGGAAACTGTTGTTTGTCAAATT
GAATCTGTACCATTTAGAGGTGCAAATTGTTCAGATGCAGCTGGTTCAGAATATGATTAT
AGTTATTTGGTTCAATTATTACAAGATCAAACTATAAACTCTTCTGCAGTTCAATGGAAT
ACTGAATGGCAATCACCTTATTTTAATTATATTGACCCTATAACTGGTAATGTTGATCAA
GTTTGGTTTGATAATCCACAATCATTATCAATTAAAGTTCAATTAGCTCAAAAATTAAAT
TTAAGAGGTGTTGCAGTTTGGAATATTGATTTTTTAGATTTTTCAGATCAATACAATTCT
CGTCCAATGTGGGATGCATTAGCTTCTTTTTTCCCACAATCAGCTTCAAGTGAACAATCA
TTAAATT
sequence update 1999. 7.28
Translated Amino Acid sequence 
ILLLFLIVFVVAQSSSSSSSSGCPCDSSYLCEPLQIAPRQEFLGFSLNSTQYPNYYWNQL
TTLAIFYETIEDELLCIAHENDVRLVWGTTFPIENLGNSSYIEEWIQEQIEKVQSTFTDG
LNFDVESPITDPTIAQQYTELVSATNKAFKAINPFYQISIDVAWSPSCIXKRCYDYAGLA
SNSDFLVAMDYDERSQVFGEKVCTAGANSSPSNALAGINNFTDLGISTDQLVMGLPWYGY
IYKNCLNGDEAGLETVVCQIESVPFRGANCSDAAGSEYDYSYLVQLLQDQTINSSAVQWN
TEWQSPYFNYIDPITGNVDQVWFDNPQSLSIKVQLAQKLNLRGVAVWNIDFLDFSDQYNS
RPMWDALASFFPQSASSEQSLN
Translated Amino Acid sequence (All Frames) 
Frame A:
ILLLFLIVFVVAQSSSSSSSSGCPCDSSYLCEPLQIAPRQEFLGFSLNSTQYPNYYWNQL
TTLAIFYETIEDELLCIAHENDVRLVWGTTFPIENLGNSSYIEEWIQEQIEKVQSTFTDG
LNFDVESPITDPTIAQQYTELVSATNKAFKAINPFYQISIDVAWSPSCIXKRCYDYAGLA
SNSDFLVAMDYDERSQVFGEKVCTAGANSSPSNALAGINNFTDLGISTDQLVMGLPWYGY
IYKNCLNGDEAGLETVVCQIESVPFRGANCSDAAGSEYDYSYLVQLLQDQTINSSAVQWN
TEWQSPYFNYIDPITGNVDQVWFDNPQSLSIKVQLAQKLNLRGVAVWNIDFLDFSDQYNS
RPMWDALASFFPQSASSEQSLN


Frame B:
fycff*lfslllkvhhhhplqdvhvihhiyvnhfklhqdksf*vfh*iarniqiiigin*
qhlryfmkqlkmnyyvlhmkmmld*ygvqhfqlkilviqvilkngfknklkkfkvhlqmv
*ilmlkvqlqiqpslnniqn*yqqlikhlkqlihsikfqsm*hghhhaxinvvmimlv*l
qipisl*qwtmmrevkflvrrcvllvqilhpqmpwlv*iilli*vlalinw*wvyhgmvi
firiv*mvmkqvwkllfvklnlyhlevqivqmqlvqnmiiviwfnyykikl*tllqfngi
lngnhliliiltl*lvmlikfgliihnhyqlkfn*lkn*i*evlqfgilif*ifqintil
vqcgmh*llfshnqlqvnnh*i


Frame C:
fiaffncfrccskfiiiilfrmsm*fiifm*ttsnctktrvfrffik*haisklllesin
ntcdil*nn*r*iimyct*k*c*ismgynisn*ksw*fkly*rmdsrtn*ksskyiyrwf
kf*c*ksnyrsnhrstiyrisisn**si*sn*silsnfnrcsmvtimhx*tll*lcwfsf
kfrfpcsngl**ekssfw*egvycwckfftlkclgwyk*fy*fry*h*signgftmvwly
l*elfkw**srfgncclsn*icti*rcklfrcswfri*l*lfgsiitrsnyklfcssmey
*maitlf*ly*pynw*c*sslv**stiiin*ssisskikfkrccsley*ffrffrsiqfs
snvgcisfffptisfk*tiik
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLE328 (SLE328Q) /CSM/SL/SLE3-B/SLE328Q.Seq.d/                       2234   0.0
CFC160 (CFC160Q) /CSM/CF/CFC1-C/CFC160Q.Seq.d/                       2190   0.0
VFD280 (VFD280Q) /CSM/VF/VFD2-D/VFD280Q.Seq.d/                       2187   0.0
VFD246 (VFD246Q) /CSM/VF/VFD2-B/VFD246Q.Seq.d/                       2183   0.0
VFA394 (VFA394Q) /CSM/VF/VFA3-D/VFA394Q.Seq.d/                       2131   0.0
VFG115 (VFG115Q) /CSM/VF/VFG1-A/VFG115Q.Seq.d/                       1312   0.0
VSB169 (VSB169Q) /CSM/VS/VSB1-C/VSB169Q.Seq.d/                       1215   0.0
VFB745 (VFB745Q) /CSM/VF/VFB7-B/VFB745Q.Seq.d/                       1207   0.0
VFN432 (VFN432Q) /CSM/VF/VFN4-B/VFN432Q.Seq.d/                       1193   0.0
VFN686 (VFN686Q) /CSM/VF/VFN6-D/VFN686Q.Seq.d/                       1187   0.0
own update 2001.11.27
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC116956) Dictyostelium discoideum chromosome 2 map 1418423...  1651   0.0    1
(BJ430477) Dictyostelium discoideum cDNA clone:ddv7k12, 3' e...  1467   0.0    1
(BJ373955) Dictyostelium discoideum cDNA clone:ddc5g15, 3' e...  1439   0.0    1
(BJ430609) Dictyostelium discoideum cDNA clone:ddv7o20, 3' e...  1419   0.0    1
(BJ428973) Dictyostelium discoideum cDNA clone:ddv1l23, 3' e...  1374   0.0    2
(AU034786) Dictyostelium discoideum slug cDNA, clone SLE328.     1346   0.0    1
(BJ431190) Dictyostelium discoideum cDNA clone:ddv13m03, 3' ...  1312   0.0    1
(AU061457) Dictyostelium discoideum slug cDNA, clone SLE328.     1259   0.0    1
(BJ410802) Dictyostelium discoideum cDNA clone:ddv1l23, 5' e...  1225   0.0    1
(AU261865) Dictyostelium discoideum vegetative cDNA clone:VS...  1215   0.0    1
dna update 2009. 3.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q8R242) RecName: Full=Di-N-acetylchitobiase;          EC=3.2.1....   256   1e-66
BC088895_1(BC088895|pid:none) Xenopus tropicalis hypothetical LO...   254   3e-66
AY260731_1(AY260731|pid:none) Xenopus laevis putative di-N-acety...   246   1e-63
AB168353_1(AB168353|pid:none) Macaca fascicularis testis cDNA cl...   246   1e-63
BC164759_1(BC164759|pid:none) Danio rerio chitobiase, di-N-acety...   242   2e-62
BC095064_1(BC095064|pid:none) Danio rerio chitobiase, di-N-acety...   242   2e-62
AK314640_1(AK314640|pid:none) Homo sapiens cDNA, FLJ95483, highl...   241   3e-62
BC072723_1(BC072723|pid:none) Danio rerio chitobiase, di-N-acety...   240   8e-62
BC022594_1(BC022594|pid:none) Mus musculus chitobiase, di-N-acet...   224   6e-57
AC115592_28(AC115592|pid:none) Dictyostelium discoideum chromoso...   223   1e-56
protein update 2009. 7. 5
PSORT 

 psg: 1.01  gvh: 0.77  alm: 0.45  top: 0.40  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: extracellular, including cell wall
	  8.0 %: mitochondrial
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoplasmic
	  4.0 %: Golgi
	  4.0 %: nuclear

>> prediction for SLE328 is exc
5' end seq. ID SLE328F
5' end seq. 
>SLE328F.Seq
ATTTTATTGCTTTTTTTAATTGTTTTCGTTGTTGCTCAAAGTTCATCATCATCATCCTCT
TCAGGATGTCCATGTGATTCATCATATTTATGTGAACCACTTCAAATTGCACCAAGACAA
GAGTTTTTAGGTTTTTCATTAAATAGCACGCAATATCCAAATTATTATTGGAATCAATTA
ACAACACTTGCGATATTTTATGAAACAATTGAAGATGAATTATTATGTATTGCACATGAA
AATGATGTTAGATTAGTATGGGGTACAACATTTCCAATTGAAAATCTTGGTAATTCAAGT
TATATTGAAGAATGGATTCAAGAACAAATTGAAAAAGTTCAAAGTACATTTACAGATGGT
TTAAATTTTGATGTTGAAAGTCCAATTACAGATCCAACCATCGCTCAACAATATACAGAA
TTAGTATCAGCAACTAATAAAGCATTTAAAGCAATTAATCCATTCTATCAAATTTCAATC
GATGTAGCATGGTCACCATCATGCATTTATAAACGTTGTTATGATTATGCTGGTTTAGCT
TCAAATTCCGATTTCCTTGTAGCAATGGACTATGATGAGAGAAGTCAAGTTTTTGGTGAG
AAGGTGTGTACTGCTGGTGCAAATTCTTCACCCTCAAATGCCTTGGCTGGTATAA-----
-----
Length of 5' end seq. 655
3' end seq. ID SLE328Z
3' end seq. 
>SLE328Z.Seq
----------TATCAAATTTCAATCGATGTAGCATGGTCACCATCATGCATTGATAAACG
TTGTTATGATTATGCTGGTTTAGCTTCAAATTCCGATTTCCTTGTAGCAATGGACTATGA
TGAGAGAAGTCAAGTTTTTGGTGAGAAGGTGTGTACTGCTGGTGCAAATTCTTCACCCTC
AAATGCCTTGGCTGGTATAAATAATTTTACTGATTTAGGTATTAGCACTGATCAATTGGT
AATGGGTTTACCATGGTATGGTTATATTTATAAGAATTGTTTAAATGGTGATGAAGCAGG
TTTGGAAACTGTTGTTTGTCAAATTGAATCTGTACCATTTAGAGGTGCAAATTGTTCAGA
TGCAGCTGGTTCAGAATATGATTATAGTTATTTGGTTCAATTATTACAAGATCAAACTAT
AAACTCTTCTGCAGTTCAATGGAATACTGAATGGCAATCACCTTATTTTAATTATATTGA
CCCTATAACTGGTAATGTTGATCAAGTTTGGTTTGATAATCCACAATCATTATCAATTAA
AGTTCAATTAGCTCAAAAATTAAATTTAAGAGGTGTTGCAGTTTGGAATATTGATTTTTT
AGATTTTTCAGATCAATACAATTCTCGTCCAATGTGGGATGCATTAGCTTCTTTTTTCCC
ACAATCAGCTTCAAGTGAACAATCATTAAATT
Length of 3' end seq. 682
Connected seq. ID SLE328P
Connected seq. 
>SLE328P.Seq
ATTTTATTGCTTTTTTTAATTGTTTTCGTTGTTGCTCAAAGTTCATCATCATCATCCTCT
TCAGGATGTCCATGTGATTCATCATATTTATGTGAACCACTTCAAATTGCACCAAGACAA
GAGTTTTTAGGTTTTTCATTAAATAGCACGCAATATCCAAATTATTATTGGAATCAATTA
ACAACACTTGCGATATTTTATGAAACAATTGAAGATGAATTATTATGTATTGCACATGAA
AATGATGTTAGATTAGTATGGGGTACAACATTTCCAATTGAAAATCTTGGTAATTCAAGT
TATATTGAAGAATGGATTCAAGAACAAATTGAAAAAGTTCAAAGTACATTTACAGATGGT
TTAAATTTTGATGTTGAAAGTCCAATTACAGATCCAACCATCGCTCAACAATATACAGAA
TTAGTATCAGCAACTAATAAAGCATTTAAAGCAATTAATCCATTCTATCAAATTTCAATC
GATGTAGCATGGTCACCATCATGCATTTATAAACGTTGTTATGATTATGCTGGTTTAGCT
TCAAATTCCGATTTCCTTGTAGCAATGGACTATGATGAGAGAAGTCAAGTTTTTGGTGAG
AAGGTGTGTACTGCTGGTGCAAATTCTTCACCCTCAAATGCCTTGGCTGGTATAA-----
-----TATCAAATTTCAATCGATGTAGCATGGTCACCATCATGCATTGATAAACGTTGTT
ATGATTATGCTGGTTTAGCTTCAAATTCCGATTTCCTTGTAGCAATGGACTATGATGAGA
GAAGTCAAGTTTTTGGTGAGAAGGTGTGTACTGCTGGTGCAAATTCTTCACCCTCAAATG
CCTTGGCTGGTATAAATAATTTTACTGATTTAGGTATTAGCACTGATCAATTGGTAATGG
GTTTACCATGGTATGGTTATATTTATAAGAATTGTTTAAATGGTGATGAAGCAGGTTTGG
AAACTGTTGTTTGTCAAATTGAATCTGTACCATTTAGAGGTGCAAATTGTTCAGATGCAG
CTGGTTCAGAATATGATTATAGTTATTTGGTTCAATTATTACAAGATCAAACTATAAACT
CTTCTGCAGTTCAATGGAATACTGAATGGCAATCACCTTATTTTAATTATATTGACCCTA
TAACTGGTAATGTTGATCAAGTTTGGTTTGATAATCCACAATCATTATCAATTAAAGTTC
AATTAGCTCAAAAATTAAATTTAAGAGGTGTTGCAGTTTGGAATATTGATTTTTTAGATT
TTTCAGATCAATACAATTCTCGTCCAATGTGGGATGCATTAGCTTCTTTTTTCCCACAAT
CAGCTTCAAGTGAACAATCATTAAATT
Length of connected seq. 1337
Full length Seq ID SLE328E
Full length Seq. 
>SLE328E.Seq
ATTTTATTGCTTTTTTTAATTGTTTTCGTTGTTGCTCAAAGTTCATCATCATCATCCTCT
TCAGGATGTCCATGTGATTCATCATATTTATGTGAACCACTTCAAATTGCACCAAGACAA
GAGTTTTTAGGTTTTTCATTAAATAGCACGCAATATCCAAATTATTATTGGAATCAATTA
ACAACACTTGCGATATTTTATGAAACAATTGAAGATGAATTATTATGTATTGCACATGAA
AATGATGTTAGATTAGTATGGGGTACAACATTTCCAATTGAAAATCTTGGTAATTCAAGT
TATATTGAAGAATGGATTCAAGAACAAATTGAAAAAGTTCAAAGTACATTTACAGATGGT
TTAAATTTTGATGTTGAAAGTCCAATTACAGATCCAACCATCGCTCAACAATATACAGAA
TTAGTATCAGCAACTAATAAAGCATTTAAAGCAATTAATCCATTCTATCAAATTTCAATC
GATGTAGCATGGTCACCATCATGCATTNATAAACGTTGTTATGATTATGCTGGTTTAGCT
TCAAATTCCGATTTCCTTGTAGCAATGGACTATGATGAGAGAAGTCAAGTTTTTGGTGAG
AAGGTGTGTACTGCTGGTGCAAATTCTTCACCCTCAAATGCCTTGGCTGGTATAAATAAT
TTTACTGATTTAGGTATTAGCACTGATCAATTGGTAATGGGTTTACCATGGTATGGTTAT
ATTTATAAGAATTGTTTAAATGGTGATGAAGCAGGTTTGGAAACTGTTGTTTGTCAAATT
GAATCTGTACCATTTAGAGGTGCAAATTGTTCAGATGCAGCTGGTTCAGAATATGATTAT
AGTTATTTGGTTCAATTATTACAAGATCAAACTATAAACTCTTCTGCAGTTCAATGGAAT
ACTGAATGGCAATCACCTTATTTTAATTATATTGACCCTATAACTGGTAATGTTGATCAA
GTTTGGTTTGATAATCCACAATCATTATCAATTAAAGTTCAATTAGCTCAAAAATTAAAT
TTAAGAGGTGTTGCAGTTTGGAATATTGATTTTTTAGATTTTTCAGATCAATACAATTCT
CGTCCAATGTGGGATGCATTAGCTTCTTTTTTCCCACAATCAGCTTCAAGTGAACAATCA
TTAAATT
Length of full length seq. 1147