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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLE286
Library SL
(Link to library)
Clone ID SLE286
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig -
Original site URL
Representative seq. ID SLE286P
(Link to Original site)
Representative DNA sequence 
>SLE286 (SLE286Q) /CSM/SL/SLE2-D/SLE286Q.Seq.d/
CACATAACAATAAAAAAAAAATAAAAAAAAACAAATAAATAATATTGTAATAGTAATAAT
AATAACAATAATAAAATAATAGTAAATAGAATAACTATTAATTTATAAAAAAAAAAAAGA
AAAAAAAAAAATGTCAAAAGAAAAAACATCTTGGGGTTCAATAAAAACAAATTTTGCAAG
AACTAAACATAAGGTTTTAGCATCATTTGGTGCTGCAGAAACTACTGTCGATAGTAAAAT
TGTTGCAGAAAAAGAAAAATTATTCACCCTTTTTAAATTAATGAAAAGATTAAATAAAAA
TGTTGATAAATATGAAGCAACTTTAAAAGACATCAATATTTTACAAAATGACATTTCACA
TGATTTATTAGCACTTAATGAGAATGACCCAGCAATTAAAGCTTATCAAGAGGTATCAAC
ATCACTTGAACAAGAAAGACTTAGAATTGACGACATTTNAGAAAATTACTACCATGATCC
ATTAAGAGTTTATTTATCACAATTCAGAGATATTAGACCTCGTCTTGAAGAATTGGNTCT
TAGAAGATTAGATATGGATAGAXXXXXXXXXXTTTACAAGTCCAAAGGAATTTAGTAGTA
ATGGTAGCACACCAACTCAAAGACCAAGAGCTTCAACTATTGCAACTCCTTCACCATCTT
CATCATCATCATATGACTCTTCACCTTATTCAACCACTACCACCACTACAACCACTACAA
ATAACTCTGTTAATTTATCGAAACCAAGTTATGGAGTACCATCACCACAACAACAACATT
ATCCACCACAACAATATCAATCATCGCCACAACAACAAGATGAAGTTCCTTACTCTTCAC
CACACGTTCAACCACAACCATCTATCAATTTACATAAACCAACAGTTAATACATCACCAG
TCAATGGTGGATCAGGTCGTCCATTACCAACACCTGCTACAAATTCAGGTGTTATTCTCA
ACAAACCTAGATCAACAACCATGGCTAGTGGTCGTCCATTACCAACACCAGCACCAGCAC
CAACAACTAAACGTGTTCAAGCACTTTATGATTTCCAAGGTCAAGATTCAACTGAATTAT
CATTTAAAAAAGGTGATATGATTACAGTTTTCAAACAAAATGGTGAATGGTGATGGATGC
TGAACTTAATGGTGTTAAAGGTTTAGTTCCATGTAATCATACAAGTGGTTAGGAAAAAAA
AAAT
sequence update 1999. 1.19
Translated Amino Acid sequence 
t*q*kknkkkqinniviviiitiik***ie*LLIYKKKKEKKKMSKEKTSWGSIKTNFAR
TKHKVLASFGAAETTVDSKIVAEKEKLFTLFKLMKRLNKNVDKYEATLKDINILQNDISH
DLLALNENDPAIKAYQEVSTSLEQERLRIDDIXENYYHDPLRVYLSQFRDIRPRLEELXL
RRLDMDR---

---FTSPKEFSSNGSTPTQRPRASTIATPSPSSSSSYDSSPYSTTTTTTTTTNNSVNLSK
PSYGVPSPQQQHYPPQQYQSSPQQQDEVPYSSPHVQPQPSINLHKPTVNTSPVNGGSGRP
LPTPATNSGVILNKPRSTTMASGRPLPTPAPAPTTKRVQALYDFQGQDSTELSFKKGDMI
TVFKQNGEW*wmlnlmvlkv*fhviiqvvrkkk
Translated Amino Acid sequence (All Frames) 
Frame A:
hitikkk*kktnk*ycnsnnnnnnkiivnritinl*kkkrkkknvkrknilgfnknkfck
n*t*gfsiiwccrnycr**nccrkrkiihpf*inekik*kc**i*snfkrhqyftk*hft
*fist**e*psn*slsrginit*trkt*n*rhxrkllp*sikslfitiqry*tss*rigs
*kiryg*---

---FTSPKEFSSNGSTPTQRPRASTIATPSPSSSSSYDSSPYSTTTTTTTTTNNSVNLSK
PSYGVPSPQQQHYPPQQYQSSPQQQDEVPYSSPHVQPQPSINLHKPTVNTSPVNGGSGRP
LPTPATNSGVILNKPRSTTMASGRPLPTPAPAPTTKRVQALYDFQGQDSTELSFKKGDMI
TVFKQNGEW*wmlnlmvlkv*fhviiqvvrkkk

Frame B:
t*q*kknkkkqinniviviiitiik***ie*LLIYKKKKEKKKMSKEKTSWGSIKTNFAR
TKHKVLASFGAAETTVDSKIVAEKEKLFTLFKLMKRLNKNVDKYEATLKDINILQNDISH
DLLALNENDPAIKAYQEVSTSLEQERLRIDDIXENYYHDPLRVYLSQFRDIRPRLEELXL
RRLDMDR---

---lqvqrnlvvmvahqlkdqelqllqllhhlhhhhmtlhliqplpplqplqitlliyrn
qvmeyhhhnnniihhnninhrhnnkmkfltlhhtfnhnhlsiyinqqlihhqsmvdqvvh
yqhllqiqvlfstnldqqpwlvvvhyqhqhqhqqlnvfkhfmiskvkiqlnyhlkkvi*l
qfsnkmvngdgc*t*wc*rfssm*sykwlgkkk

Frame C:
hnnkkkikknk*iil******q**nnsk*nny*fikkkkkkkkcqkkkhlgvq*kqilqe
lnirf*hhlvlqkllsivkllqkkknyspfln**kd*ikmlinmkql*ktsifykmtfhm
iy*hlmrmtqqlklikryqhhlnkkdlelttfxkittmih*efiyhnseildlvlknwxl
ed*iwi---

---ykskgi***w*htnsktksfnycnsftifiiii*lftlfnhyhhynhyk*lc*fiet
klwstitttttlstttisiiatttr*ssllfttrstttiyqft*tns*yitsqwwirssi
tntcykfrcysqqt*innhg*wssitntststnn*tcsstl*fprsrfn*iii*kr*ydy
sfqtkw*mvmdaelngvkglvpcnhtsg*ekkn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLE286 (SLE286Q) /CSM/SL/SLE2-D/SLE286Q.Seq.d/                        833   0.0
SLB779 (SLB779Q) /CSM/SL/SLB7-D/SLB779Q.Seq.d/                        714   0.0
CFJ522 (CFJ522Q) /CSM/CF/CFJ5-A/CFJ522Q.Seq.d/                        666   0.0
SFK847 (SFK847Q) /CSM/SF/SFK8-B/SFK847Q.Seq.d/                        626   e-178
SFD758 (SFD758Q) /CSM/SF/SFD7-C/SFD758Q.Seq.d/                        622   e-177
SFD842 (SFD842Q) /CSM/SF/SFD8-B/SFD842Q.Seq.d/                        605   e-172
CFI493 (CFI493Q) /CSM/CF/CFI4-D/CFI493Q.Seq.d/                        601   e-170
CFB395 (CFB395Q) /CSM/CF/CFB3-D/CFB395Q.Seq.d/                        541   e-153
SLJ744 (SLJ744Q) /CSM/SL/SLJ7-B/SLJ744Q.Seq.d/                        456   e-127
VSD620 (VSD620Q) /CSM/VS/VSD6-A/VSD620Q.Seq.d/                        198   1e-49
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU034760) Dictyostelium discoideum slug cDNA, clone SLE286.      743   0.0    2
(BJ375825) Dictyostelium discoideum cDNA clone:ddc20k05, 3' ...   620   0.0    4
(BJ400027) Dictyostelium discoideum cDNA clone:dds8d15, 3' e...   620   0.0    2
(BJ399982) Dictyostelium discoideum cDNA clone:dds8d12, 3' e...   567   0.0    3
(BJ375214) Dictyostelium discoideum cDNA clone:ddc17j24, 3' ...   599   0.0    4
(BJ373627) Dictyostelium discoideum cDNA clone:ddc3n23, 3' e...   539   0.0    2
(BJ401193) Dictyostelium discoideum cDNA clone:dds22n12, 3' ...   589   0.0    3
(AU034131) Dictyostelium discoideum slug cDNA, clone SLB779.      620   0.0    2
(AU061446) Dictyostelium discoideum slug cDNA, clone SLE286.      486   e-177  2
(AU053819) Dictyostelium discoideum slug cDNA, clone SLJ744.      365   e-124  2
dna update 2009. 3.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A47106(A47106;B47106)myosin heavy chain ID - slime mold (Dictyos...    43   0.002
BX842684_1(BX842684|pid:none) Zebrafish DNA sequence from clone ...    44   0.015
AK293592_1(AK293592|pid:none) Homo sapiens cDNA FLJ54594 complet...    43   0.019
AK302273_1(AK302273|pid:none) Homo sapiens cDNA FLJ52941 complet...    43   0.019
AK293546_1(AK293546|pid:none) Homo sapiens cDNA FLJ53715 complet...    43   0.019
AY888447_1(AY888447|pid:none) Synthetic construct Homo sapiens c...    43   0.019
AF139493_1(AF139493|pid:none) Rattus norvegicus syndapin IIab mR...    42   0.032
AF139494_1(AF139494|pid:none) Rattus norvegicus syndapin IIba mR...    42   0.032
(Q9WVE8) RecName: Full=Protein kinase C and casein kinase substr...    42   0.042
AL022476_4(AL022476|pid:none) Human DNA sequence from clone RP3-...    42   0.042
protein update 2009. 6. 3
PSORT 

 psg: 0.46  gvh: 0.43  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for SLE286 is nuc
5' end seq. ID SLE286F
5' end seq. 
>SLE286F.Seq
CACATAACAATAAAAAAAAAATAAAAAAAAACAAATAAATAATATTGTAATAGTAATAAT
AATAACAATAATAAAATAATAGTAAATAGAATAACTATTAATTTATAAAAAAAAAAAAGA
AAAAAAAAAAATGTCAAAAGAAAAAACATCTTGGGGTTCAATAAAAACAAATTTTGCAAG
AACTAAACATAAGGTTTTAGCATCATTTGGTGCTGCAGAAACTACTGTCGATAGTAAAAT
TGTTGCAGAAAAAGAAAAATTATTCACCCTTTTTAAATTAATGAAAAGATTAAATAAAAA
TGTTGATAAATATGAAGCAACTTTAAAAGACATCAATATTTTACAAAATGACATTTCACA
TGATTTATTAGCACTTAATGAGAATGACCCAGCAATTAAAGCTTATCAAGAGGTATCAAC
ATCACTTGAACAAGAAAGACTTAGAATTGACGACATTTNAGAAAATTACTACCATGATCC
ATTAAGAGTTTATTTATCACAATTCAGAGATATTAGACCTCGTCTTGAAGAATTGGNTCT
TAGAAGATTAGATATGGATAGA----------
Length of 5' end seq. 562
3' end seq. ID SLE286Z
3' end seq. 
>SLE286Z.Seq
----------TTTACAAGTCCAAAGGAATTTAGTAGTAATGGTAGCACACCAACTCAAAG
ACCAAGAGCTTCAACTATTGCAACTCCTTCACCATCTTCATCATCATCATATGACTCTTC
ACCTTATTCAACCACTACCACCACTACAACCACTACAAATAACTCTGTTAATTTATCGAA
ACCAAGTTATGGAGTACCATCACCACAACAACAACATTATCCACCACAACAATATCAATC
ATCGCCACAACAACAAGATGAAGTTCCTTACTCTTCACCACACGTTCAACCACAACCATC
TATCAATTTACATAAACCAACAGTTAATACATCACCAGTCAATGGTGGATCAGGTCGTCC
ATTACCAACACCTGCTACAAATTCAGGTGTTATTCTCAACAAACCTAGATCAACAACCAT
GGCTAGTGGTCGTCCATTACCAACACCAGCACCAGCACCAACAACTAAACGTGTTCAAGC
ACTTTATGATTTCCAAGGTCAAGATTCAACTGAATTATCATTTAAAAAAGGTGATATGAT
TACAGTTTTCAAACAAAATGGTGAATGGTGATGGATGCTGAACTTAATGGTGTTAAAGGT
TTAGTTCCATGTAATCATACAAGTGGTTAGGAAAAAAAAAAT
Length of 3' end seq. 632
Connected seq. ID SLE286P
Connected seq. 
>SLE286P.Seq
CACATAACAATAAAAAAAAAATAAAAAAAAACAAATAAATAATATTGTAATAGTAATAAT
AATAACAATAATAAAATAATAGTAAATAGAATAACTATTAATTTATAAAAAAAAAAAAGA
AAAAAAAAAAATGTCAAAAGAAAAAACATCTTGGGGTTCAATAAAAACAAATTTTGCAAG
AACTAAACATAAGGTTTTAGCATCATTTGGTGCTGCAGAAACTACTGTCGATAGTAAAAT
TGTTGCAGAAAAAGAAAAATTATTCACCCTTTTTAAATTAATGAAAAGATTAAATAAAAA
TGTTGATAAATATGAAGCAACTTTAAAAGACATCAATATTTTACAAAATGACATTTCACA
TGATTTATTAGCACTTAATGAGAATGACCCAGCAATTAAAGCTTATCAAGAGGTATCAAC
ATCACTTGAACAAGAAAGACTTAGAATTGACGACATTTNAGAAAATTACTACCATGATCC
ATTAAGAGTTTATTTATCACAATTCAGAGATATTAGACCTCGTCTTGAAGAATTGGNTCT
TAGAAGATTAGATATGGATAGA----------TTTACAAGTCCAAAGGAATTTAGTAGTA
ATGGTAGCACACCAACTCAAAGACCAAGAGCTTCAACTATTGCAACTCCTTCACCATCTT
CATCATCATCATATGACTCTTCACCTTATTCAACCACTACCACCACTACAACCACTACAA
ATAACTCTGTTAATTTATCGAAACCAAGTTATGGAGTACCATCACCACAACAACAACATT
ATCCACCACAACAATATCAATCATCGCCACAACAACAAGATGAAGTTCCTTACTCTTCAC
CACACGTTCAACCACAACCATCTATCAATTTACATAAACCAACAGTTAATACATCACCAG
TCAATGGTGGATCAGGTCGTCCATTACCAACACCTGCTACAAATTCAGGTGTTATTCTCA
ACAAACCTAGATCAACAACCATGGCTAGTGGTCGTCCATTACCAACACCAGCACCAGCAC
CAACAACTAAACGTGTTCAAGCACTTTATGATTTCCAAGGTCAAGATTCAACTGAATTAT
CATTTAAAAAAGGTGATATGATTACAGTTTTCAAACAAAATGGTGAATGGTGATGGATGC
TGAACTTAATGGTGTTAAAGGTTTAGTTCCATGTAATCATACAAGTGGTTAGGAAAAAAA
AAAT
Length of connected seq. 1194
Full length Seq ID -
Full length Seq. -
Length of full length seq. -