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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLE274
Library SL
(Link to library)
Clone ID SLE274
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12305-1
Original site URL
Representative seq. ID SLE274P
(Link to Original site)
Representative DNA sequence 
>SLE274 (SLE274Q) /CSM/SL/SLE2-D/SLE274Q.Seq.d/
GTGAATGAGTTACACAAGAATGGTGGTCATCCACAACTTAGAACACTATTAGCAATTGGT
GGTATCGATTTACGTGAACAAGAACATATCTTTAAGAAAGGTGTTCATATGATCATTGCA
ACACCTGGTCGTTTATTAGATCTATTAAATAAAAAGAAAATCAATTTCAAACTTTGTAAA
TATCTTGGTTTAGATGAAGCTGATAGATTAATTGATTTAGGTTTTGAGGATGATATTCGT
TCAGTTTTAGATAATTTCACAAATCAAAGACAAACTCTACTCTTTAGTGCAACTATGCCA
AAGAAAATTCAAGAATTTGCTAGAAGTGCTCTAGTTTTACCAGTTGAAGTTAATGTTGGT
AGAGCTGGTGCTGCAAATTTAAATGTTACTCAAGAAGTTGAATTTGTTAAACCTGAAGCT
AAAATCGTTTATTTATTAGAATGTTTACAAAAAACACCACCACCTGTATTAATATTTTGT
GAAAATAAAAAAGATGTTGATGATATTTATGAATATTTATTATTGAAACAAGTTGAAGCA
GTTTCAATACATGGTGATAAATCACAAGATGAAAGAGAATCAGCAATTAAAGCATTTAGA
GAGGGTAAAAAGGATGTTTTGGTTGCAACAGATGTTGCATCTAAAGGTTTAGATTTTCCA
GAGATTCAACATGTCATCAATTTCGATATGCCAAGAGAGATTGXXXXXXXXXXGAATTGG
TAGAACTGGTCGTCGTGGTAATAAAGGTGTTGCACCTACATTTATCAATAAGAATAATAC
TGAATCTTTATTATTGGATCTTAAATACCTATTAATTGAAGCTAAACAAAAAGTACCACC
TGCACTACTTGAAATACCAGATGATAATCAATACCTTCAAAAACTTCAAGATCGTAATGG
AAATACTGGTGGTGGTGCTGATGATGATGATACTAAACCTTGTGAATATTGTGATGGTCG
TGGTCATAGATTAGTTAATTGTCCAAAATTGAAAAAACAAGCTGGTCCAANNAGGGACTT
TTTCGGTTCTGGTGGTGGTGATTGGT
sequence update 1999. 1.19
Translated Amino Acid sequence 
VNELHKNGGHPQLRTLLAIGGIDLREQEHIFKKGVHMIIATPGRLLDLLNKKKINFKLCK
YLGLDEADRLIDLGFEDDIRSVLDNFTNQRQTLLFSATMPKKIQEFARSALVLPVEVNVG
RAGAANLNVTQEVEFVKPEAKIVYLLECLQKTPPPVLIFCENKKDVDDIYEYLLLKQVEA
VSIHGDKSQDERESAIKAFREGKKDVLVATDVASKGLDFPEIQHVINFDMPREI---

---IGRTGRRGNKGVAPTFINKNNTESLLLDLKYLLIEAKQKVPPALLEIPDDNQYLQKL
QDRNGNTGGGADDDDTKPCEYCDGRGHRLVNCPKLKKQAGPXRDFFGSGGGDW
Translated Amino Acid sequence (All Frames) 
Frame A:
VNELHKNGGHPQLRTLLAIGGIDLREQEHIFKKGVHMIIATPGRLLDLLNKKKINFKLCK
YLGLDEADRLIDLGFEDDIRSVLDNFTNQRQTLLFSATMPKKIQEFARSALVLPVEVNVG
RAGAANLNVTQEVEFVKPEAKIVYLLECLQKTPPPVLIFCENKKDVDDIYEYLLLKQVEA
VSIHGDKSQDERESAIKAFREGKKDVLVATDVASKGLDFPEIQHVINFDMPREI---

---elvelvvvvikvlhlhlsiriilnlyywilnty*lklnkkyhlhylkyqmiintfkn
fkivmeilvvvlmmmilnlvnivmvvvid*livqn*knklvqxgtfsvlvvvig

Frame B:
*msytrmvvihnlehy*qlvvsiyvnknislrkvfi*slqhlvvy*iy*ikrksisnfvn
ilv*mklid*li*vlrmifvqf*iisqikdklyslvqlcqrkfknllevl*fyqlklmlv
elvlqi*mllkklnllnlklksfiy*nvykkhhhly*yfvkikkmlmifmniyy*nklkq
fqymvinhkmkenqqlkhlervkrmfwlqqmlhlkv*ifqrfnmssisicqerl---

---nw*nwssw**rcctyiyq*e*y*ifiigs*ipin*s*tksttctt*ntr**sipskt
srs*wkywwwc****y*tl*il*wsws*is*lskiektswsxxglfrfwww*l

Frame C:
e*vtqewwsstt*ntisnwwyrft*trtyl*ercsydhcntwsfirsik*kenqfqtl*i
swfr*s**in*frf*g*ysfsfr*fhksktnstl*cnyakensric*kcssfts*s*cw*
swcckfkcysrs*ic*t*s*nrlfirmftkntttcinil*k*krc**yl*ifiiets*ss
fntw**itr*krisn*si*rg*kgcfgcnrcci*rfrfsrdstchqfryakrd---

---IGRTGRRGNKGVAPTFINKNNTESLLLDLKYLLIEAKQKVPPALLEIPDDNQYLQKL
QDRNGNTGGGADDDDTKPCEYCDGRGHRLVNCPKLKKQAGPXRDFFGSGGGDW
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLE274 (SLE274Q) /CSM/SL/SLE2-D/SLE274Q.Seq.d/                       2002   0.0
SLD190 (SLD190Q) /CSM/SL/SLD1-D/SLD190Q.Seq.d/                        632   e-180
VFF108 (VFF108Q) /CSM/VF/VFF1-A/VFF108Q.Seq.d/                        545   e-154
VHQ315 (VHQ315Q) /CSM/VH/VHQ3-A/VHQ315Q.Seq.d/                        529   e-149
VFL366 (VFL366Q) /CSM/VF/VFL3-C/VFL366Q.Seq.d/                         50   7e-05
VFI649 (VFI649Q) /CSM/VF/VFI6-C/VFI649Q.Seq.d/                         50   7e-05
SSJ868 (SSJ868Q) /CSM/SS/SSJ8-C/SSJ868Q.Seq.d/                         50   7e-05
SFC168 (SFC168Q) /CSM/SF/SFC1-C/SFC168Q.Seq.d/                         46   0.001
AHB684 (AHB684Q) /CSM/AH/AHB6-D/AHB684Q.Seq.d/                         46   0.001
VHQ347 (VHQ347Q) /CSM/VH/VHQ3-B/VHQ347Q.Seq.d/                         42   0.017
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU061443) Dictyostelium discoideum slug cDNA, clone SLE274.     1392   0.0    1
(BJ428230) Dictyostelium discoideum cDNA clone:ddv11o01, 3' ...   543   0.0    3
(AU052358) Dictyostelium discoideum slug cDNA, clone SLD190.      630   0.0    2
(BJ446587) Dictyostelium discoideum cDNA clone:ddv63n03, 3' ...   426   0.0    3
(AU034751) Dictyostelium discoideum slug cDNA, clone SLE274.      646   0.0    1
(BI510741) BB160003B10C07.5 Bee Brain Normalized Library, BB...    84   2e-32  4
(EH305394) UAHYP_17C_R_H07 UaHyphae_ARS Uromyces appendicula...    92   5e-32  7
(DW224850) GH_HYPT_01-01-01R_B06_InvR_13Apr04_046_F Stem - 3...    78   2e-23  3
(AJ245474) Plasmodium falciparum gene for RNA helicase-1.          58   2e-23  5
(CZ542648) SRAA-aad45d03.b1 Strongyloides ratti whole genome...    60   7e-22  5
dna update 2009. 3.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54KG1) RecName: Full=Probable ATP-dependent RNA helicase ddx41...   460   e-180
AB236766_1(AB236766|pid:none) Trifolium pratense RNA for putativ...   352   e-118
AC144484_19(AC144484|pid:none) Medicago truncatula clone mth2-30...   352   e-118
AP008208_322(AP008208|pid:none) Oryza sativa (japonica cultivar-...   346   e-117
EU957034_1(EU957034|pid:none) Zea mays clone 1581050 ATP-depende...   342   e-116
GM694497_1(GM694497|pid:none) Sequence 53 from Patent EP1953235....   342   e-116
EU956781_1(EU956781|pid:none) Zea mays clone 1573776 ATP-depende...   342   e-116
(Q9LU46) RecName: Full=DEAD-box ATP-dependent RNA helicase 35;  ...   344   e-115
CP001330_157(CP001330|pid:none) Micromonas sp. RCC299 chromosome...   325   e-108
BT045656_1(BT045656|pid:none) Salmo salar clone ssal-rgf-528-002...   328   e-106
protein update 2009. 6. 3
PSORT 

 psg: 0.55  gvh: 0.45  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.04
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: vesicles of secretory system
	  4.0 %: vacuolar

>> prediction for SLE274 is cyt
5' end seq. ID SLE274F
5' end seq. 
>SLE274F.Seq
GTGAATGAGTTACACAAGAATGGTGGTCATCCACAACTTAGAACACTATTAGCAATTGGT
GGTATCGATTTACGTGAACAAGAACATATCTTTAAGAAAGGTGTTCATATGATCATTGCA
ACACCTGGTCGTTTATTAGATCTATTAAATAAAAAGAAAATCAATTTCAAACTTTGTAAA
TATCTTGGTTTAGATGAAGCTGATAGATTAATTGATTTAGGTTTTGAGGATGATATTCGT
TCAGTTTTAGATAATTTCACAAATCAAAGACAAACTCTACTCTTTAGTGCAACTATGCCA
AAGAAAATTCAAGAATTTGCTAGAAGTGCTCTAGTTTTACCAGTTGAAGTTAATGTTGGT
AGAGCTGGTGCTGCAAATTTAAATGTTACTCAAGAAGTTGAATTTGTTAAACCTGAAGCT
AAAATCGTTTATTTATTAGAATGTTTACAAAAAACACCACCACCTGTATTAATATTTTGT
GAAAATAAAAAAGATGTTGATGATATTTATGAATATTTATTATTGAAACAAGTTGAAGCA
GTTTCAATACATGGTGATAAATCACAAGATGAAAGAGAATCAGCAATTAAAGCATTTAGA
GAGGGTAAAAAGGATGTTTTGGTTGCAACAGATGTTGCATCTAAAGGTTTAGATTTTCCA
GAGATTCAACATGTCATCAATTTCGATATGCCAAGAGAGATTG----------
Length of 5' end seq. 703
3' end seq. ID SLE274Z
3' end seq. 
>SLE274Z.Seq
----------GAATTGGTAGAACTGGTCGTCGTGGTAATAAAGGTGTTGCACCTACATTT
ATCAATAAGAATAATACTGAATCTTTATTATTGGATCTTAAATACCTATTAATTGAAGCT
AAACAAAAAGTACCACCTGCACTACTTGAAATACCAGATGATAATCAATACCTTCAAAAA
CTTCAAGATCGTAATGGAAATACTGGTGGTGGTGCTGATGATGATGATACTAAACCTTGT
GAATATTGTGATGGTCGTGGTCATAGATTAGTTAATTGTCCAAAATTGAAAAAACAAGCT
GGTCCAANNAGGGACTTTTTCGGTTCTGGTGGTGGTGATTGGT
Length of 3' end seq. 333
Connected seq. ID SLE274P
Connected seq. 
>SLE274P.Seq
GTGAATGAGTTACACAAGAATGGTGGTCATCCACAACTTAGAACACTATTAGCAATTGGT
GGTATCGATTTACGTGAACAAGAACATATCTTTAAGAAAGGTGTTCATATGATCATTGCA
ACACCTGGTCGTTTATTAGATCTATTAAATAAAAAGAAAATCAATTTCAAACTTTGTAAA
TATCTTGGTTTAGATGAAGCTGATAGATTAATTGATTTAGGTTTTGAGGATGATATTCGT
TCAGTTTTAGATAATTTCACAAATCAAAGACAAACTCTACTCTTTAGTGCAACTATGCCA
AAGAAAATTCAAGAATTTGCTAGAAGTGCTCTAGTTTTACCAGTTGAAGTTAATGTTGGT
AGAGCTGGTGCTGCAAATTTAAATGTTACTCAAGAAGTTGAATTTGTTAAACCTGAAGCT
AAAATCGTTTATTTATTAGAATGTTTACAAAAAACACCACCACCTGTATTAATATTTTGT
GAAAATAAAAAAGATGTTGATGATATTTATGAATATTTATTATTGAAACAAGTTGAAGCA
GTTTCAATACATGGTGATAAATCACAAGATGAAAGAGAATCAGCAATTAAAGCATTTAGA
GAGGGTAAAAAGGATGTTTTGGTTGCAACAGATGTTGCATCTAAAGGTTTAGATTTTCCA
GAGATTCAACATGTCATCAATTTCGATATGCCAAGAGAGATTG----------GAATTGG
TAGAACTGGTCGTCGTGGTAATAAAGGTGTTGCACCTACATTTATCAATAAGAATAATAC
TGAATCTTTATTATTGGATCTTAAATACCTATTAATTGAAGCTAAACAAAAAGTACCACC
TGCACTACTTGAAATACCAGATGATAATCAATACCTTCAAAAACTTCAAGATCGTAATGG
AAATACTGGTGGTGGTGCTGATGATGATGATACTAAACCTTGTGAATATTGTGATGGTCG
TGGTCATAGATTAGTTAATTGTCCAAAATTGAAAAAACAAGCTGGTCCAANNAGGGACTT
TTTCGGTTCTGGTGGTGGTGATTGGT
Length of connected seq. 1036
Full length Seq ID -
Full length Seq. -
Length of full length seq. -