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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLE160
Library SL
(Link to library)
Clone ID SLE160
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID SLE160P
(Link to Original site)
Representative DNA sequence 
>SLE160 (SLE160Q) /CSM/SL/SLE1-C/SLE160Q.Seq.d/
GTTTAATAAAAAAGAAAGAATACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTT
TTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGG
GAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTT
CATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGAT
GCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTA
GTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAG
TTATTACCATCCAAATTAGATCAATCAGCATTCGATGGTGAATCTGAATATTCCATTATG
TTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGA
AAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAA
TACACTTTGGTCATTTCACCAGATAACACCTACAAAGTACTCGTCGATAACAAAGAGATC
CAAGCAGGTAACCTCGCTGATGATTGGGAATTACTTCCATCAAAACAAATCXXXXXXXXX
XCCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCC
AGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATG
GGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAA
TGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAA
TATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGC
CAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAG
AAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGC
TGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGACAAAAAAGA
AGAAGTCAAAAAATCAACCAAAAAAGTTGATCATGACGAACTTTAAATGAAATAAATAAA
TAATAAAAAAAAAACAAATAAAAAAAAAACTCGTGTTAAACTAAAAAAA
sequence update 1999. 1.19
Translated Amino Acid sequence 
v**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHKEDGKSGKLV
HTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKVDCGGSYIK
LLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEINKVETDQLTHQ
YTLVISPDNTYKVLVDNKEIQAGNLADDWELLPSKQI---

---PEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNELY
LFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKKMFDDLEAAKA
EERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTDDKKEEVKKSTKKVDHDEL*mk
*innkkktnkkktrvklkk
Translated Amino Acid sequence (All Frames) 
Frame A:
v**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHKEDGKSGKLV
HTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKVDCGGSYIK
LLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEINKVETDQLTHQ
YTLVISPDNTYKVLVDNKEIQAGNLADDWELLPSKQI---

---PEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNELY
LFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKKMFDDLEAAKA
EERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTDDKKEEVKKSTKKVDHDEL*mk
*innkkktnkkktrvklkk

Frame B:
fnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllvigikkmvnqvn*f
ilqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*rmnkklivvvhtss
yyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srkkstklkpin*lin
tlwsfhqitptkyssitkrskqvtslmignyfhqnk---

---qrigmkkttvngkpqpspiqntkvngkqrksqiqntkvngyth*litqntlktmnst
slmisvlsvsnygklnlvpslti*ssptlskklkislkrpllptkkpkrkclttskppkl
krerkltkn*lpkkllkkkpkklmkkkkkslkki*lklmtkkkksknqpkklimtnfk*n
k*iikkkqikkklvln*k

Frame C:
likkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**la*rrw*ir*iss
ycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke*tks*lwwfihqv
itiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqernqqs*nrsinssi
hfghftr*hlqstrr*qrdpsr*pr**lgitsiktn---

---rgle*rrrr*mgspnhrqsriqr*meskenpksriqr*mgtpin**prir*rq*tlp
l**srcyrfrimas*iwfhl*qydrhrlcrrs*rfl*kdlccqprsrkenv*rprsrqs*
rekes*rkiscrksc*krsrrs**rrrrsr*rrfs*n**qkrrsqkinqks*s*rtlnei
nk**kknk*kknsc*tkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLE160 (SLE160Q) /CSM/SL/SLE1-C/SLE160Q.Seq.d/                       1822   0.0
AFN528 (AFN528Q) /CSM/AF/AFN5-B/AFN528Q.Seq.d/                       1142   0.0
AFK286 (AFK286Q) /CSM/AF/AFK2-D/AFK286Q.Seq.d/                       1142   0.0
AFK245 (AFK245Q) /CSM/AF/AFK2-B/AFK245Q.Seq.d/                       1142   0.0
AFJ522 (AFJ522Q) /CSM/AF/AFJ5-A/AFJ522Q.Seq.d/                       1142   0.0
AFG846 (AFG846Q) /CSM/AF/AFG8-B/AFG846Q.Seq.d/                       1142   0.0
AFE410 (AFE410Q) /CSM/AF/AFE4-A/AFE410Q.Seq.d/                       1142   0.0
AFE318 (AFE318Q) /CSM/AF/AFE3-A/AFE318Q.Seq.d/                       1142   0.0
AFE168 (AFE168Q) /CSM/AF/AFE1-C/AFE168Q.Seq.d/                       1142   0.0
AFD463 (AFD463Q) /CSM/AF/AFD4-C/AFD463Q.Seq.d/                       1142   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU061416) Dictyostelium discoideum slug cDNA, clone SLE160.     1142   0.0    1
(BJ325824) Dictyostelium discoideum cDNA clone:dda2a16, 5' e...  1142   0.0    1
(U36937) Dictyostelium discoideum calreticulin mRNA, complet...  1134   0.0    1
(BJ327915) Dictyostelium discoideum cDNA clone:dda22p03, 5' ...  1118   0.0    1
(BJ324695) Dictyostelium discoideum cDNA clone:dda7d16, 5' e...  1116   0.0    1
(BJ328475) Dictyostelium discoideum cDNA clone:dda28g07, 5' ...  1112   0.0    1
(BJ412320) Dictyostelium discoideum cDNA clone:ddv7j22, 5' e...  1110   0.0    1
(BJ325422) Dictyostelium discoideum cDNA clone:dda14l12, 5' ...  1110   0.0    1
(BJ325359) Dictyostelium discoideum cDNA clone:dda14d06, 5' ...  1110   0.0    1
(BJ323427) Dictyostelium discoideum cDNA clone:dda10d18, 5' ...  1110   0.0    1
dna update 2009. 3.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   397   e-109
BT072764_1(BT072764|pid:none) Salmo salar clone ssal-rgf-539-230...   226   6e-90
U27698_1(U27698|pid:none) Arabidopsis thaliana calreticulin (AtC...   247   5e-64
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   247   7e-64
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   246   2e-63
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   246   2e-63
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   244   3e-63
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   244   3e-63
AY086745_1(AY086745|pid:none) Arabidopsis thaliana clone 27210 m...   244   5e-63
AC003114_8(AC003114|pid:none) Arabidopsis thaliana chromosome 1 ...   244   5e-63
protein update 2009. 6. 4
PSORT 

 psg: 1.01  gvh: 0.69  alm: 0.47  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.06  erl: 1.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: extracellular, including cell wall
	 32.0 %: endoplasmic reticulum
	 12.0 %: cytoplasmic
	 12.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: nuclear

>> prediction for SLE160 is exc
5' end seq. ID SLE160F
5' end seq. 
>SLE160F.Seq
GTTTAATAAAAAAGAAAGAATACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTT
TTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGG
GAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTT
CATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGAT
GCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTA
GTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAG
TTATTACCATCCAAATTAGATCAATCAGCATTCGATGGTGAATCTGAATATTCCATTATG
TTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGA
AAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAA
TACACTTTGGTCATTTCACCAGATAACACCTACAAAGTACTCGTCGATAACAAAGAGATC
CAAGCAGGTAACCTCGCTGATGATTGGGAATTACTTCCATCAAAACAAATC---------
-
Length of 5' end seq. 651
3' end seq. ID SLE160Z
3' end seq. 
>SLE160Z.Seq
----------CCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCAT
CGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAA
AGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACTCTA
CCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCAT
CTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGAC
CTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGC
TGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGC
CGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGA
CAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGTTGATCATGACGAACTTTAAATGAA
ATAAATAAATAATAAAAAAAAAACAAATAAAAAAAAAACTCGTGTTAAACTAAAAAAA
Length of 3' end seq. 588
Connected seq. ID SLE160P
Connected seq. 
>SLE160P.Seq
GTTTAATAAAAAAGAAAGAATACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTT
TTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGG
GAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTT
CATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGAT
GCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTA
GTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAG
TTATTACCATCCAAATTAGATCAATCAGCATTCGATGGTGAATCTGAATATTCCATTATG
TTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGA
AAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAA
TACACTTTGGTCATTTCACCAGATAACACCTACAAAGTACTCGTCGATAACAAAGAGATC
CAAGCAGGTAACCTCGCTGATGATTGGGAATTACTTCCATCAAAACAAATC---------
-CCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCC
AGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATG
GGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAA
TGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAA
TATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGC
CAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAG
AAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGC
TGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGACAAAAAAGA
AGAAGTCAAAAAATCAACCAAAAAAGTTGATCATGACGAACTTTAAATGAAATAAATAAA
TAATAAAAAAAAAACAAATAAAAAAAAAACTCGTGTTAAACTAAAAAAA
Length of connected seq. 1239
Full length Seq ID -
Full length Seq. -
Length of full length seq. -