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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLE152
Library SL
(Link to library)
Clone ID SLE152
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID SLE152P
(Link to Original site)
Representative DNA sequence 
>SLE152 (SLE152Q) /CSM/SL/SLE1-C/SLE152Q.Seq.d/
ATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTCGCACCAGAAGAACAC
CCAGTTCTCTTAACTGAAGCCCCATTAAATCCAAAAGCTAACAGAGAAAAAATGACTCAA
ATTATGTTTGAAACTTTCAACACTCCAGCCATGTACGTTGCCATTCAAGCCGTCTTATCA
TTATATGCATCTGGTCGTACCACCGGTATCGTCATGGATTCAGGTGATGGTGTCTCTCAC
ACTGTTCCAATTTATGAAGGTTATGCCTTACCACACGCCATCTTACGTTTAGATTTAGCT
GGTCGTGATCTTACTGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACC
ACCACTGCTGAAAGAGAAATTGTCAGAGATATTAAAGAGAAATTAGCCTACGTCGCTCTC
GACTTTGAAGCTGAAATGCAAACTGCTGCTTCATCATCAGCATTAGAAAAATCATACGAA
TTACCAGACGGTCAAGTTATCACAATCGGTAATGAACGTTTCCGTTGTCCAGAAGCCCTC
TTCCAACCATCATTCTTAGGTATGGAATCAGCTGGTATCCACGAAACCACCTACAATTCC
ATCATGAAATGTGATGTCGATATCCGTAAAGATTTATACGGTAATGTCGTCTTATCAGGT
GGTACAACTATGTTCCXXXXXXXXXXTGGATCTATTTTAGCTTCACTCTCAACTTTCCAA
CAAATGTGGATCTCCAAAGAAGAATATGACGAATCAGGTCCATCAATTGTCCACAGAAAA
TGTTTCTAAACAATTAAAACTAGTGATGAAAGTGCTTCTCACACAAATAAATATATTTAA
ATGTATAATAATAATAAAATATAATCTTATTTTTAGTGGTTGTTGATCTTTATCCAGCCA
TCAAAAAAAAAAAAAAAAAATAAAATAATTCCTTATTTTTAAAAATATAT
sequence update 1999. 1.19
Translated Amino Acid sequence 
MEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLS
LYASGRTTGIVMDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYMMKILTERGYSFT
TTAEREIVRDIKEKLAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEAL
FQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMF---

---GSILASLSTFQQMWISKEEYDESGPSIVHRKCF*tiktsdesashtnkyi*mynnnk
i*syf*wllifiqpskkkkknkiipyf*ky
Translated Amino Acid sequence (All Frames) 
Frame A:
MEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLS
LYASGRTTGIVMDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYMMKILTERGYSFT
TTAEREIVRDIKEKLAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEAL
FQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMF---

---wiyfsftlnfptnvdlqrri*rirsincpqkmflnn*n***kcfshk*iylnv****
niilflvvvdlypaikkkkkk*nnslflkiy

Frame B:
wkksgiilstmnsvshqkntqfs*lkph*iqkltekk*lklclklstlqpctlpfkpsyh
ymhlvvppvsswiqvmvsltlfqfmkvmpyhtpsyv*i*lvvillit**ks*lnvvthsp
pllkeklseilkrn*ptslstlklkcklllhhqh*knhtnyqtvklsqsvmnvsvvqkps
snhhs*vwnqlvstkpptips*nvmsisvkiytvmssyqvvqlcs---

---GSILASLSTFQQMWISKEEYDESGPSIVHRKCF*tiktsdesashtnkyi*mynnnk
i*syf*wllifiqpskkkkknkiipyf*ky

Frame C:
gknlasyflq*tpcrtrrtpssln*spiksks*qrkndsnyv*nfqhsshvrchssrlii
iciwsyhryrhgfr*wclshcsnl*rlclttrhltfrfsws*sy*lhdenln*twllihh
hc*krncqry*reislrrsrl*s*nanccfiisirkiiritrrssyhnr**tfplsrspl
ptiilrygiswyprnhlqfhhem*cryp*rfir*crlirwynyv---

---dlf*lhsqlsnkcgspkknmtnqvhqlstenvskqlklvmkvlltqinifkciiiik
ynlifsgc*slsshqkkkkkik*flifkni
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLE152 (SLE152Q) /CSM/SL/SLE1-C/SLE152Q.Seq.d/                       1574   0.0
VFD357 (VFD357Q) /CSM/VF/VFD3-C/VFD357Q.Seq.d/                       1326   0.0
VFD343 (VFD343Q) /CSM/VF/VFD3-B/VFD343Q.Seq.d/                       1285   0.0
VFL794 (VFL794Q) /CSM/VF/VFL7-D/VFL794Q.Seq.d/                       1279   0.0
VFD511 (VFD511Q) /CSM/VF/VFD5-A/VFD511Q.Seq.d/                       1245   0.0
VFA470 (VFA470Q) /CSM/VF/VFA4-C/VFA470Q.Seq.d/                       1245   0.0
VFM627 (VFM627Q) /CSM/VF/VFM6-B/VFM627Q.Seq.d/                       1126   0.0
VFF617 (VFF617Q) /CSM/VF/VFF6-A/VFF617Q.Seq.d/                       1126   0.0
VFF595 (VFF595Q) /CSM/VF/VFF5-D/VFF595Q.Seq.d/                       1126   0.0
SFG794 (SFG794Q) /CSM/SF/SFG7-D/SFG794Q.Seq.d/                       1126   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC116986) Dictyostelium discoideum chromosome 2 map 2234041...  1340   0.0    1
(AU061412) Dictyostelium discoideum slug cDNA, clone SLE152.     1340   0.0    1
(AC116957) Dictyostelium discoideum chromosome 2 map 1685067...  1126   0.0    1
(DL184440) Protein expression method.                            1086   0.0    1
(DL184438) Protein expression method.                            1086   0.0    1
(M14146) D.discoideum actin 15 gene, complete cds.               1086   0.0    1
(AC115680) Dictyostelium discoideum chromosome 2 map 4915084...  1086   0.0    1
(X03281) Dictyostelium discoideum gene for actin A8.             1063   0.0    2
(AC116956) Dictyostelium discoideum chromosome 2 map 1418423...  1039   0.0    6
(AU284282) Dictyostelium discoideum gamete cDNA clone:FC-AH1...  1031   0.0    4
dna update 2009. 3.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   500   e-140
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        499   e-140
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   498   e-139
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   497   e-139
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   497   e-139
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   496   e-139
AF508263_1(AF508263|pid:none) Pyrocystis lunula actin mRNA, comp...   493   e-138
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          492   e-138
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   491   e-137
EU004601_1(EU004601|pid:none) Sappinia pedata strain NZ05-11L-1 ...   489   e-137
protein update 2009. 6. 3
PSORT 

 psg: 0.74  gvh: 0.38  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	 16.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall

>> prediction for SLE152 is cyt
5' end seq. ID SLE152F
5' end seq. 
>SLE152F.Seq
ATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTCGCACCAGAAGAACAC
CCAGTTCTCTTAACTGAAGCCCCATTAAATCCAAAAGCTAACAGAGAAAAAATGACTCAA
ATTATGTTTGAAACTTTCAACACTCCAGCCATGTACGTTGCCATTCAAGCCGTCTTATCA
TTATATGCATCTGGTCGTACCACCGGTATCGTCATGGATTCAGGTGATGGTGTCTCTCAC
ACTGTTCCAATTTATGAAGGTTATGCCTTACCACACGCCATCTTACGTTTAGATTTAGCT
GGTCGTGATCTTACTGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACC
ACCACTGCTGAAAGAGAAATTGTCAGAGATATTAAAGAGAAATTAGCCTACGTCGCTCTC
GACTTTGAAGCTGAAATGCAAACTGCTGCTTCATCATCAGCATTAGAAAAATCATACGAA
TTACCAGACGGTCAAGTTATCACAATCGGTAATGAACGTTTCCGTTGTCCAGAAGCCCTC
TTCCAACCATCATTCTTAGGTATGGAATCAGCTGGTATCCACGAAACCACCTACAATTCC
ATCATGAAATGTGATGTCGATATCCGTAAAGATTTATACGGTAATGTCGTCTTATCAGGT
GGTACAACTATGTTCC----------
Length of 5' end seq. 676
3' end seq. ID SLE152Z
3' end seq. 
>SLE152Z.Seq
----------TGGATCTATTTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCA
AAGAAGAATATGACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTCTAAACAATTA
AAACTAGTGATGAAAGTGCTTCTCACACAAATAAATATATTTAAATGTATAATAATAATA
AAATATAATCTTATTTTTAGTGGTTGTTGATCTTTATCCAGCCATCAAAAAAAAAAAAAA
AAAATAAAATAATTCCTTATTTTTAAAAATATAT
Length of 3' end seq. 264
Connected seq. ID SLE152P
Connected seq. 
>SLE152P.Seq
ATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTCGCACCAGAAGAACAC
CCAGTTCTCTTAACTGAAGCCCCATTAAATCCAAAAGCTAACAGAGAAAAAATGACTCAA
ATTATGTTTGAAACTTTCAACACTCCAGCCATGTACGTTGCCATTCAAGCCGTCTTATCA
TTATATGCATCTGGTCGTACCACCGGTATCGTCATGGATTCAGGTGATGGTGTCTCTCAC
ACTGTTCCAATTTATGAAGGTTATGCCTTACCACACGCCATCTTACGTTTAGATTTAGCT
GGTCGTGATCTTACTGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACC
ACCACTGCTGAAAGAGAAATTGTCAGAGATATTAAAGAGAAATTAGCCTACGTCGCTCTC
GACTTTGAAGCTGAAATGCAAACTGCTGCTTCATCATCAGCATTAGAAAAATCATACGAA
TTACCAGACGGTCAAGTTATCACAATCGGTAATGAACGTTTCCGTTGTCCAGAAGCCCTC
TTCCAACCATCATTCTTAGGTATGGAATCAGCTGGTATCCACGAAACCACCTACAATTCC
ATCATGAAATGTGATGTCGATATCCGTAAAGATTTATACGGTAATGTCGTCTTATCAGGT
GGTACAACTATGTTCC----------TGGATCTATTTTAGCTTCACTCTCAACTTTCCAA
CAAATGTGGATCTCCAAAGAAGAATATGACGAATCAGGTCCATCAATTGTCCACAGAAAA
TGTTTCTAAACAATTAAAACTAGTGATGAAAGTGCTTCTCACACAAATAAATATATTTAA
ATGTATAATAATAATAAAATATAATCTTATTTTTAGTGGTTGTTGATCTTTATCCAGCCA
TCAAAAAAAAAAAAAAAAAATAAAATAATTCCTTATTTTTAAAAATATAT
Length of connected seq. 940
Full length Seq ID -
Full length Seq. -
Length of full length seq. -