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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLE147
Library SL
(Link to library)
Clone ID SLE147
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID SLE147E
(Link to Original site)
Representative DNA sequence 
>SLE147 (SLE147Q) /CSM/SL/SLE1-B/SLE147Q.Seq.d/
CGGTAATGTCGTCTTATCTGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAA
CAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCACCAGA
ACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACA
AATGTGGATCTCCAAAGAAGAATACGACGAATCAGGTCCATCAATTGTCCACAGAAAATG
TTTCTAAACAATTAAAACTAGTGATGAAAGTGCTTCTCACAAATATTTATGTAAAATATA
TAATAAAATACATTTAATATTTTTAGGTTTTGTTTAGTTGTTGATCTTTATCCGACTATT
TAAAATCTAAATAATAATTTGT
sequence update 1998. 5.17
Translated Amino Acid sequence 
GNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQ
MWISKEEYDESGPSIVHRKCF*tiktsdesashkylckiynkihliflgfv*llifirlf
ki*iiic
Translated Amino Acid sequence (All Frames) 
Frame A:
r*crliwwynyvpryc*syeqrincfspinhen*nhcstrt*ilcldwwiylsftlnfpt
nvdlqrrirrirsincpqkmflnn*n***kcfsqifm*ni**ntfnifrfclvvdlypti
*nlnnnl


Frame B:
GNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQ
MWISKEEYDESGPSIVHRKCF*tiktsdesashkylckiynkihliflgfv*llifirlf
ki*iiic


Frame C:
vmssylvvqlcsqvlliv*tkn*ll*phqp*klksllhqnvntlsglvdls*lhsqlsnk
cgspkknttnqvhqlstenvskqlklvmkvlltniyvkyiikyi*yf*vlfsc*slsdyl
ksk**f
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLI570 (SLI570Q) /CSM/SL/SLI5-C/SLI570Q.Seq.d/                        757   0.0
SLE773 (SLE773Q) /CSM/SL/SLE7-D/SLE773Q.Seq.d/                        757   0.0
SLE147 (SLE147Q) /CSM/SL/SLE1-B/SLE147Q.Seq.d/                        757   0.0
SLA822 (SLA822Q) /CSM/SL/SLA8-A/SLA822Q.Seq.d/                        757   0.0
SLA325 (SLA325Q) /CSM/SL/SLA3-B/SLA325Q.Seq.d/                        757   0.0
VFH871 (VFH871Q) /CSM/VF/VFH8-C/VFH871Q.Seq.d/                        726   0.0
VFG355 (VFG355Q) /CSM/VF/VFG3-C/VFG355Q.Seq.d/                        726   0.0
VFH614 (VFH614Q) /CSM/VF/VFH6-A/VFH614Q.Seq.d/                        720   0.0
AFB472 (AFB472Q) /CSM/AF/AFB4-C/AFB472Q.Seq.d/                        720   0.0
SFJ706 (SFJ706Q) /CSM/SF/SFJ7-A/SFJ706Q.Seq.d/                        716   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC116957) Dictyostelium discoideum chromosome 2 map 1685067...   757   0.0    1
(AU053403) Dictyostelium discoideum slug cDNA, clone SLI570.      757   0.0    1
(AU040052) Dictyostelium discoideum slug cDNA, clone SLA325.      757   0.0    1
(AU039573) Dictyostelium discoideum slug cDNA, clone SLE773.      757   0.0    1
(AU034661) Dictyostelium discoideum slug cDNA, clone SLE147.      757   0.0    1
(AU033435) Dictyostelium discoideum slug cDNA, clone SLA822.      757   0.0    1
(BJ434376) Dictyostelium discoideum cDNA clone:ddv16n18, 3' ...   676   0.0    2
(BJ431301) Dictyostelium discoideum cDNA clone:ddv13n13, 3' ...   676   0.0    2
(BJ434185) Dictyostelium discoideum cDNA clone:ddv16k04, 3' ...   676   0.0    2
(BJ340762) Dictyostelium discoideum cDNA clone:dda3p18, 3' e...   676   0.0    2
dna update 2009. 3.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   166   2e-40
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   166   2e-40
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   166   2e-40
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        166   2e-40
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   165   5e-40
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   164   9e-40
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   164   9e-40
K02960_1(K02960|pid:none) Dictyostelium discoideum actin 8 gene,...   164   1e-39
(Q554S6) RecName: Full=Actin-17; AltName: Full=Actin-2-sub 2;  &...   162   5e-39
AF508263_1(AF508263|pid:none) Pyrocystis lunula actin mRNA, comp...   161   6e-39
protein update 2009. 7. 5
PSORT 

 psg: 0.91  gvh: 0.49  alm: 0.46  top: 0.47  tms: 0.00  mit: 0.21  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: cytoskeletal
	 12.0 %: mitochondrial
	  8.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for SLE147 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID SLE147E
Full length Seq. 
>SLE147E.Seq
CGGTAATGTCGTCTTATCTGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAA
CAAAGAATTAACTGCTTTAGCCCCATCAACCATGAAAATTAAAATCATTGCTCCACCAGA
ACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACA
AATGTGGATCTCCAAAGAAGAATACGACGAATCAGGTCCATCAATTGTCCACAGAAAATG
TTTCTAAACAATTAAAACTAGTGATGAAAGTGCTTCTCACAAATATTTATGTAAAATATA
TAATAAAATACATTTAATATTTTTAGGTTTTGTTTAGTTGTTGATCTTTATCCGACTATT
TAAAATCTAAATAATAATTTGT
Length of full length seq. 382