>SLE112 (SLE112Q) /CSM/SL/SLE1-A/SLE112Q.Seq.d/
CAGATTTTGGTTTCACTTTACCAAAGATAAATTTAATTTATGGTTCAAACCAACCAATGG
GTCCACCACCAAATCAACAACAACAACAATCATCTTCCCCTTCACAATATATGAACTCTA
ATAATAACAATAATAATAATAACAATAATAATAATAATGGTAATAATAATAATGGTAATA
ATAATAGTAATAATAATAATAANNNNNTTTTACCACAATATCACCAACAACAACATCAAC
AACAACAACAACAAATGCCTCACCACCAACAACACCAACAGCACCAACAACCACAACATC
AACAAATGTCCCCACATCATCATCATCAACAACAACAAATGCCCCACCATCATCAACAAC
AACAACAACATCAACAACAACAACAACAACAACAACAACAACAACAACAAATGTCACCAC
ATCACCAACAACAAATGTCACCACATCACCAACAACAACATCCACAAATGTCGCCACATC
ACCAACAAATGTCTCATCACCATCATCAACAACAACTACAACAACAACAACAACACCAAA
TGCAACAACCACAACATCAACAAATGCTACAACCATCAAAAGTTATTTACGTTCCAAAAG
GTTCACCATATATGCXXXXXXXXXX

DFGFTLPKINLIYGSNQPMGPPPNQQQQQSSSPSQYMNSNNNNNNNNNNNNNGNNNNGNN
NSNNNNXXXLPQYHQQQHQQQQQQMPHHQQHQQHQQPQHQQMSPHHHHQQQQMPHHHQQQ
QQHQQQQQQQQQQQQQMSPHHQQQMSPHHQQQHPQMSPHHQQMSHHHHQQQLQQQQQHQM
QQPQHQQMLQPSKVIYVPKGSPYM---

Frame A:
qilvslyqr*i*fmvqtnqwvhhqinnnnnhlplhni*tliitiiiitiiiimviiimvi
iiviiiixxfyhnitnnninnnnnkclttnntnstnnhninkcphiiiinnnkcptiinn
nnninnnnnnnnnnnnkchhitnnkchhitnnnihkcrhitnkclitiinnnynnnnntk
cnnhninkcynhqklftfqkvhhi---


Frame B:
rfwfhftkdkfnlwfkptngsttksttttiifpftiyel***q****q****w****w**
*******xxfttisptttstttttnasppttptapttttstnvptsssstttnappsstt
tttsttttttttttttnvttspttnvttsptttstnvatsptnvsspsstttttttttpn
attttstnattiksylrskrftiy---


Frame C:
DFGFTLPKINLIYGSNQPMGPPPNQQQQQSSSPSQYMNSNNNNNNNNNNNNNGNNNNGNN
NSNNNNXXXLPQYHQQQHQQQQQQMPHHQQHQQHQQPQHQQMSPHHHHQQQQMPHHHQQQ
QQHQQQQQQQQQQQQQMSPHHQQQMSPHHQQQHPQMSPHHQQMSHHHHQQQLQQQQQHQM
QQPQHQQMLQPSKVIYVPKGSPYM---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLE112 (SLE112Q) /CSM/SL/SLE1-A/SLE112Q.Seq.d/                        234   1e-60
SSG119 (SSG119Q) /CSM/SS/SSG1-A/SSG119Q.Seq.d/                         46   6e-04
SLK791 (SLK791Q) /CSM/SL/SLK7-D/SLK791Q.Seq.d/                         46   6e-04
CHI264 (CHI264Q) /CSM/CH/CHI2-C/CHI264Q.Seq.d/                         46   6e-04
VSB504 (VSB504Q) /CSM/VS/VSB5-A/VSB504Q.Seq.d/                         44   0.003
VHO159 (VHO159Q) /CSM/VH/VHO1-C/VHO159Q.Seq.d/                         44   0.003
VHJ685 (VHJ685Q) /CSM/VH/VHJ6-D/VHJ685Q.Seq.d/                         44   0.003
SHC167 (SHC167Q) /CSM/SH/SHC1-C/SHC167Q.Seq.d/                         44   0.003
CHJ685 (CHJ685Q) /CSM/CH/CHJ6-D/CHJ685Q.Seq.d/                         44   0.003
AFN845 (AFN845Q) /CSM/AF/AFN8-B/AFN845Q.Seq.d/                         44   0.003

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU061402) Dictyostelium discoideum slug cDNA, clone SLE112.      234   1e-77  4
(EW701548) EST01288 Hemp Uni-ZAP XR cDNA library Cannabis sa...    58   4e-04  1
(C90033) Dictyostelium discoideum slug cDNA, clone SSG119.         46   0.15   2
(CT009525) Mouse DNA sequence from clone RP23-65F10 on chrom...    46   0.17   2
(AC134987) Mus musculus BAC clone RP23-117E24 from 14, compl...    46   0.18   2
(AC155572) Zea mays strain B73 clone ZMMBBc0176G09, *** SEQU...    48   0.39   1
(AC155568) Zea mays strain B73 clone ZMMBBc0172N20, *** SEQU...    48   0.39   1
(AC155483) Zea mays strain B73 clone ZMMBBc0010F22, *** SEQU...    48   0.39   1
(AC203958) Zea mays chromosome 3 clone CH201-10F22; ZMMBBc00...    48   0.39   1
(AC190748) Zea mays chromosome 3 clone ZMMBBb-325I2; ZMMBBb0...    48   0.39   1

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3159261
Number of Hits to DB: 189,484,402

 psg: 0.75  gvh: 0.44  alm: 0.59  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 32.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  8.0 %: vacuolar

>> prediction for SLE112 is nuc

>SLE112F.Seq
CAGATTTTGGTTTCACTTTACCAAAGATAAATTTAATTTATGGTTCAAACCAACCAATGG
GTCCACCACCAAATCAACAACAACAACAATCATCTTCCCCTTCACAATATATGAACTCTA
ATAATAACAATAATAATAATAACAATAATAATAATAATGGTAATAATAATAATGGTAATA
ATAATAGTAATAATAATAATAANNNNNTTTTACCACAATATCACCAACAACAACATCAAC
AACAACAACAACAAATGCCTCACCACCAACAACACCAACAGCACCAACAACCACAACATC
AACAAATGTCCCCACATCATCATCATCAACAACAACAAATGCCCCACCATCATCAACAAC
AACAACAACATCAACAACAACAACAACAACAACAACAACAACAACAACAAATGTCACCAC
ATCACCAACAACAAATGTCACCACATCACCAACAACAACATCCACAAATGTCGCCACATC
ACCAACAAATGTCTCATCACCATCATCAACAACAACTACAACAACAACAACAACACCAAA
TGCAACAACCACAACATCAACAAATGCTACAACCATCAAAAGTTATTTACGTTCCAAAAG
GTTCACCATATATGC----------