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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLD812
Library SL
(Link to library)
Clone ID SLD812
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16182-1
Original site URL
Representative seq. ID SLD812P
(Link to Original site)
Representative DNA sequence 
>SLD812 (SLD812Q) /CSM/SL/SLD8-A/SLD812Q.Seq.d/
AATGACAATGACGAAATCGAAGTCTACTATATGCAAGCACCAGTTTTCGAAGCAGTTTTT
GGAAATTTCTTTGGTAAGTTGGGTGGATATCATTCAGCAATTGGTTTCTATGACCTTACA
ACTGGTTTAAATTATACAGCAGAATATGATGCTTTCTTTGAAGTAGGAAATGGTACATTA
CCAAATATCATAAATGTCGATGGTAAAGATGAACTTTTATGGTGTAATGCTGGTATXXXX
XXXXXXGCAATTTTATTCATCTCAATTNGGTTATTTTAAAATTNGGTTATTTTAAATTAA
ATTGGTTATAAATAAAAACTAAAAACCAAAATTATAAAAAAAAAATATCTTTTTTTTTTT
AATATTTTTCAAACTGTTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGATAG
TTTCTTTTTTTATTTTCACTTTTTTTTTATTTTGTTTTGAGAAA
sequence update 1999. 2.23
Translated Amino Acid sequence 
NDNDEIEVYYMQAPVFEAVFGNFFGKLGGYHSAIGFYDLTTGLNYTAEYDAFFEVGNGTL
PNIINVDGKDELLWCNAG---

---NFIHLNXVILKXGYFKLNWL*iktknqnykkkisfff*yfsncf*kkkkkkkkkkti
vsffiftfflfcfek
Translated Amino Acid sequence (All Frames) 
Frame A:
NDNDEIEVYYMQAPVFEAVFGNFFGKLGGYHSAIGFYDLTTGLNYTAEYDAFFEVGNGTL
PNIINVDGKDELLWCNAG---

---ailfisixlf*nxviln*igyk*klktkiikkkylfffnifqtvsekkkkkkkkkkr
*flflfslffyfvlr

Frame B:
mtmtkskstickhqfskqfleislvswvdiiqqlvsmtlqlv*iiqqnmmlslk*emvhy
qis*msmvkmnfygvmlv---

---qfyssqxgyfkixlf*iklvinkn*kpkl*kknifffliffklflkkkkkkkkkknd
sfffyfhfffilf*e

Frame C:
*q*rnrsllyastsfrssfwkflw*vgwisfsnwfl*pynwfklysri*cfl*srkwyit
kyhkcrw*r*tfmv*cwy---

---NFIHLNXVILKXGYFKLNWL*iktknqnykkkisfff*yfsncf*kkkkkkkkkkti
vsffiftfflfcfek
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLD812 (SLD812Q) /CSM/SL/SLD8-A/SLD812Q.Seq.d/                        541   e-153
VHL857 (VHL857Q) /CSM/VH/VHL8-C/VHL857Q.Seq.d/                        468   e-131
VFL220 (VFL220Q) /CSM/VF/VFL2-A/VFL220Q.Seq.d/                        468   e-131
VFJ281 (VFJ281Q) /CSM/VF/VFJ2-D/VFJ281Q.Seq.d/                        468   e-131
VFH286 (VFH286Q) /CSM/VF/VFH2-D/VFH286Q.Seq.d/                        468   e-131
VFG895 (VFG895Q) /CSM/VF/VFG8-D/VFG895Q.Seq.d/                        468   e-131
VFC480 (VFC480Q) /CSM/VF/VFC4-D/VFC480Q.Seq.d/                        468   e-131
SSG340 (SSG340Q) /CSM/SS/SSG3-B/SSG340Q.Seq.d/                        468   e-131
SSB231 (SSB231Q) /CSM/SS/SSB2-B/SSB231Q.Seq.d/                        468   e-131
SSA660 (SSA660Q) /CSM/SS/SSA6-C/SSA660Q.Seq.d/                        468   e-131
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC116305) Dictyostelium discoideum chromosome 2 map 1005175...   468   e-130  3
(AU076369) Dictyostelium discoideum slug cDNA, clone SSA660.      468   e-128  1
(AU061512) Dictyostelium discoideum slug cDNA, clone SLE504.      468   e-128  1
(AU061351) Dictyostelium discoideum slug cDNA, clone SLD812.      468   e-128  1
(BJ425060) Dictyostelium discoideum cDNA clone:ddv54b16, 5' ...   468   e-128  1
(BJ417874) Dictyostelium discoideum cDNA clone:ddv15k22, 5' ...   468   e-128  1
(BJ415600) Dictyostelium discoideum cDNA clone:ddv23g06, 5' ...   468   e-128  1
(BJ414588) Dictyostelium discoideum cDNA clone:ddv19a22, 5' ...   468   e-128  1
(BJ413283) Dictyostelium discoideum cDNA clone:ddv14n24, 5' ...   468   e-128  1
(BJ411824) Dictyostelium discoideum cDNA clone:ddv5p20, 5' e...   468   e-128  1
dna update 2009. 3.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q553W9) RecName: Full=Ceroid-lipofuscinosis neuronal protein 5 ...   169   3e-41
AC116305_28(AC116305|pid:none) Dictyostelium discoideum chromoso...   169   3e-41
AF068227_1(AF068227|pid:none) Homo sapiens putative transmembran...    65   5e-10
CR761663_1(CR761663|pid:none) Xenopus tropicalis finished cDNA, ...    65   5e-10
AK032293_1(AK032293|pid:none) Mus musculus adult male olfactory ...    65   9e-10
BC025487_1(BC025487|pid:none) Mus musculus ceroid-lipofuscinosis...    65   9e-10
AK075109_1(AK075109|pid:none) Homo sapiens cDNA FLJ90628 fis, cl...    64   2e-09
AY885123_1(AY885123|pid:none) Canis familiaris CLN5 (CLN5) gene,...    63   4e-09
(Q5JZQ9) Ceroid-lipofuscinosis neuronal protein 5 precursor (Pro...    63   4e-09
(Q1ZYR0) RecName: Full=Ceroid-lipofuscinosis neuronal protein 5;...    62   8e-09
protein update 2009. 6. 2
PSORT 

 psg: 0.75  gvh: 0.41  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: vacuolar
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal

>> prediction for SLD812 is cyt
5' end seq. ID SLD812F
5' end seq. 
>SLD812F.Seq
AATGACAATGACGAAATCGAAGTCTACTATATGCAAGCACCAGTTTTCGAAGCAGTTTTT
GGAAATTTCTTTGGTAAGTTGGGTGGATATCATTCAGCAATTGGTTTCTATGACCTTACA
ACTGGTTTAAATTATACAGCAGAATATGATGCTTTCTTTGAAGTAGGAAATGGTACATTA
CCAAATATCATAAATGTCGATGGTAAAGATGAACTTTTATGGTGTAATGCTGGTAT----
------
Length of 5' end seq. 236
3' end seq. ID SLD812Z
3' end seq. 
>SLD812Z.Seq
----------GCAATTTTATTCATCTCAATTNGGTTATTTTAAAATTNGGTTATTTTAAA
TTAAATTGGTTATAAATAAAAACTAAAAACCAAAATTATAAAAAAAAAATATCTTTTTTT
TTTTAATATTTTTCAAACTGTTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACG
ATAGTTTCTTTTTTTATTTTCACTTTTTTTTTATTTTGTTTTGAGAAA
Length of 3' end seq. 218
Connected seq. ID SLD812P
Connected seq. 
>SLD812P.Seq
AATGACAATGACGAAATCGAAGTCTACTATATGCAAGCACCAGTTTTCGAAGCAGTTTTT
GGAAATTTCTTTGGTAAGTTGGGTGGATATCATTCAGCAATTGGTTTCTATGACCTTACA
ACTGGTTTAAATTATACAGCAGAATATGATGCTTTCTTTGAAGTAGGAAATGGTACATTA
CCAAATATCATAAATGTCGATGGTAAAGATGAACTTTTATGGTGTAATGCTGGTAT----
------GCAATTTTATTCATCTCAATTNGGTTATTTTAAAATTNGGTTATTTTAAATTAA
ATTGGTTATAAATAAAAACTAAAAACCAAAATTATAAAAAAAAAATATCTTTTTTTTTTT
AATATTTTTCAAACTGTTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGATAG
TTTCTTTTTTTATTTTCACTTTTTTTTTATTTTGTTTTGAGAAA
Length of connected seq. 454
Full length Seq ID -
Full length Seq. -
Length of full length seq. -