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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLD531
Library SL
(Link to library)
Clone ID SLD531
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16311-1
Original site URL
Representative seq. ID SLD531P
(Link to Original site)
Representative DNA sequence 
>SLD531 (SLD531Q) /CSM/SL/SLD5-B/SLD531Q.Seq.d/
GTTTTATCTGCCTTATGCGTTTTATTAGTCAGTGTTGCCACTGCCAAACAACAATTATCC
GAAGTTGAATACAGAAATGCTTTCACCAATTGGATGATTGCTCACCAAAGACATTATTCA
TCAGAAGAGTTCAATGGTCGTTACAATATCTTCAAAGCCAATATGGATTACGTCAATGAA
TGGAATACCAAAGGTAGTGAAACCGTTTTAGGTTTAAATGTCTTTGCTGATATCAGCAAT
GAAGAATACAGAGCCACCTATTTAGGTACCCCATTCGATGCTTCATCCCTCGAAATGACC
GAATCCGATAAAATCTTCGATGXXXXXXXXXXTGGTGTTTATCCAACCGCTGGTGACTAC
TGGATCGTTAAAAACTCATGGGGTACCTCATGGGGTATGGATGGTTACATTTTAATGACC
AAAGGAAATAACAATCAATGCGGTATTGCAACAATGGCCTCACGTCCAACTGCTGTTGCA
TCACTTTAAATTAACGATACATATATATACATATTTATATAATTTTATAATTAATAAAAA
ATAATACGAAAACATATAAAATTTATATGATAAATAAATA
sequence update 1999. 2.23
Translated Amino Acid sequence 
VLSALCVLLVSVATAKQQLSEVEYRNAFTNWMIAHQRHYSSEEFNGRYNIFKANMDYVNE
WNTKGSETVLGLNVFADISNEEYRATYLGTPFDASSLEMTESDKIFD---

---GVYPTAGDYWIVKNSWGTSWGMDGYILMTKGNNNQCGIATMASRPTAVASL*indty
iyifi*fyn**kiirkhikfi**in
Translated Amino Acid sequence (All Frames) 
Frame A:
VLSALCVLLVSVATAKQQLSEVEYRNAFTNWMIAHQRHYSSEEFNGRYNIFKANMDYVNE
WNTKGSETVLGLNVFADISNEEYRATYLGTPFDASSLEMTESDKIFD---

---wclsnrw*lldr*klmgylmgygwlhfndqrk*qsmrycnngltsncccitln*ryi
yihiyiil*liknntktykiymink

Frame B:
fylpyafy*svlplpnnnypklntemlspig*lltkdiihqkssmvvtisskpiwitsmn
gipkvvkpf*v*msllisamknteppi*vphsmlhpsk*pnpikssm---

---GVYPTAGDYWIVKNSWGTSWGMDGYILMTKGNNNQCGIATMASRPTAVASL*indty
iyifi*fyn**kiirkhikfi**in

Frame C:
ficlmrfisqcchcqttiirs*iqkcfhqlddcspktlfirrvqwslqylqsqyglrq*m
eyqr**nrfrfkclc*yqq*riqshlfrypircfiprndrir*nlr---

---vfiqplvttgslkthgvphgvwmvtf**pkeitinavlqqwphvqlllhhfkltihi
ytylynfiinkk*yeni*nlydk*i
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHJ815 (VHJ815Q) /CSM/VH/VHJ8-A/VHJ815Q.Seq.d/                        638   0.0
VHJ755 (VHJ755Q) /CSM/VH/VHJ7-C/VHJ755Q.Seq.d/                        638   0.0
VHI101 (VHI101Q) /CSM/VH/VHI1-A/VHI101Q.Seq.d/                        638   0.0
VHC273 (VHC273Q) /CSM/VH/VHC2-D/VHC273Q.Seq.d/                        638   0.0
VHB771 (VHB771Q) /CSM/VH/VHB7-C/VHB771Q.Seq.d/                        638   0.0
VHB683 (VHB683Q) /CSM/VH/VHB6-D/VHB683Q.Seq.d/                        638   0.0
VFO832 (VFO832Q) /CSM/VF/VFO8-B/VFO832Q.Seq.d/                        638   0.0
VFO544 (VFO544Q) /CSM/VF/VFO5-B/VFO544Q.Seq.d/                        638   0.0
VFN695 (VFN695Q) /CSM/VF/VFN6-D/VFN695Q.Seq.d/                        638   0.0
VFN617 (VFN617Q) /CSM/VF/VFN6-A/VFN617Q.Seq.d/                        638   0.0
own update 2004. 7.27
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(U72746) Dictyostelium discoideum cysteine proteinase (cprG)...   636   0.0    2
(AU061203) Dictyostelium discoideum slug cDNA, clone SLD531.      636   e-178  1
(BJ423874) Dictyostelium discoideum cDNA clone:ddv50n13, 5' ...   636   e-178  1
(BJ423723) Dictyostelium discoideum cDNA clone:ddv50n04, 5' ...   636   e-178  1
(BJ422764) Dictyostelium discoideum cDNA clone:ddv47a01, 5' ...   636   e-178  1
(BJ419318) Dictyostelium discoideum cDNA clone:ddv35a20, 5' ...   636   e-178  1
(BJ419027) Dictyostelium discoideum cDNA clone:ddv34e22, 5' ...   636   e-178  1
(BJ418989) Dictyostelium discoideum cDNA clone:ddv34n17, 5' ...   636   e-178  1
(BJ417820) Dictyostelium discoideum cDNA clone:ddv28m24, 5' ...   636   e-178  1
(BJ417810) Dictyostelium discoideum cDNA clone:ddv28k23, 5' ...   636   e-178  1
dna update 2009. 3.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q94504) RecName: Full=Cysteine proteinase 7;          EC=3.4.22...   200   2e-50
(Q94503) RecName: Full=Cysteine proteinase 6;          EC=3.4.22...   184   1e-45
L36204_1(L36204|pid:none) Dictyostelium discoideum cysteine prot...   154   1e-36
(P54640) RecName: Full=Cysteine proteinase 5;          EC=3.4.22...   152   5e-36
L36205_1(L36205|pid:none) Dictyostelium discoideum cysteine prot...   147   2e-34
(P04989) RecName: Full=Cysteine proteinase 2;          EC=3.4.22...   110   3e-23
AC117076_20(AC117076|pid:none) Dictyostelium discoideum chromoso...   106   5e-22
EU143709_1(EU143709|pid:none) Stichopus japonicus cathepsin L mR...    77   3e-13
X03930_1(X03930|pid:none) Dictyostelium discoideum 3'gene fragme...    74   2e-12
EF066525_1(EF066525|pid:none) Radix peregra cathepsin-L-like cys...    73   6e-12
protein update 2009. 6. 2
PSORT 

 psg: 0.95  gvh: 0.81  alm: 0.50  top: 0.47  tms: 0.00  mit: 0.28  mip: 0.06
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: extracellular, including cell wall
	 16.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoskeletal
	  4.0 %: nuclear

>> prediction for SLD531 is exc
5' end seq. ID SLD531F
5' end seq. 
>SLD531F.Seq
GTTTTATCTGCCTTATGCGTTTTATTAGTCAGTGTTGCCACTGCCAAACAACAATTATCC
GAAGTTGAATACAGAAATGCTTTCACCAATTGGATGATTGCTCACCAAAGACATTATTCA
TCAGAAGAGTTCAATGGTCGTTACAATATCTTCAAAGCCAATATGGATTACGTCAATGAA
TGGAATACCAAAGGTAGTGAAACCGTTTTAGGTTTAAATGTCTTTGCTGATATCAGCAAT
GAAGAATACAGAGCCACCTATTTAGGTACCCCATTCGATGCTTCATCCCTCGAAATGACC
GAATCCGATAAAATCTTCGATG----------
Length of 5' end seq. 322
3' end seq. ID SLD531Z
3' end seq. 
>SLD531Z.Seq
----------TGGTGTTTATCCAACCGCTGGTGACTACTGGATCGTTAAAAACTCATGGG
GTACCTCATGGGGTATGGATGGTTACATTTTAATGACCAAAGGAAATAACAATCAATGCG
GTATTGCAACAATGGCCTCACGTCCAACTGCTGTTGCATCACTTTAAATTAACGATACAT
ATATATACATATTTATATAATTTTATAATTAATAAAAAATAATACGAAAACATATAAAAT
TTATATGATAAATAAATA
Length of 3' end seq. 248
Connected seq. ID SLD531P
Connected seq. 
>SLD531P.Seq
GTTTTATCTGCCTTATGCGTTTTATTAGTCAGTGTTGCCACTGCCAAACAACAATTATCC
GAAGTTGAATACAGAAATGCTTTCACCAATTGGATGATTGCTCACCAAAGACATTATTCA
TCAGAAGAGTTCAATGGTCGTTACAATATCTTCAAAGCCAATATGGATTACGTCAATGAA
TGGAATACCAAAGGTAGTGAAACCGTTTTAGGTTTAAATGTCTTTGCTGATATCAGCAAT
GAAGAATACAGAGCCACCTATTTAGGTACCCCATTCGATGCTTCATCCCTCGAAATGACC
GAATCCGATAAAATCTTCGATG----------TGGTGTTTATCCAACCGCTGGTGACTAC
TGGATCGTTAAAAACTCATGGGGTACCTCATGGGGTATGGATGGTTACATTTTAATGACC
AAAGGAAATAACAATCAATGCGGTATTGCAACAATGGCCTCACGTCCAACTGCTGTTGCA
TCACTTTAAATTAACGATACATATATATACATATTTATATAATTTTATAATTAATAAAAA
ATAATACGAAAACATATAAAATTTATATGATAAATAAATA
Length of connected seq. 570
Full length Seq ID -
Full length Seq. -
Length of full length seq. -