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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLD504
Library SL
(Link to library)
Clone ID SLD504
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID SLD504P
(Link to Original site)
Representative DNA sequence 
>SLD504 (SLD504Q) /CSM/SL/SLD5-A/SLD504Q.Seq.d/
GGTGAAGATGTTCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGTTTT
GCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACT
GGTGTTATGGTCGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCAAAG
AGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATTGTTACCAACTGGGATGAT
ATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGAAGAACAC
CCAGTTCTCTTAACTGAAGCCCXXXXXXXXXXAGAACGTAAATACTCTGTCTGGATTGGT
GGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATAT
GACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTCTAAACAATTAAAACTAGTGAT
GAAAGTGCTTCTCACACAAACAAATATATTTAAATGTATAATAATAATAAAATATAATTA
TTTTTAATGGTTGTTGATCTTTATCCGACTATCA
sequence update 1999. 2.23
Translated Amino Acid sequence 
GEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDSYVGDEAQSK
RGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEA---

---ERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIVHRKCF*tiktsdesashtnk
yi*mynnnki*lflmvvdlypti
Translated Amino Acid sequence (All Frames) 
Frame A:
GEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDSYVGDEAQSK
RGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEA---

---rt*ilcldwwiylsftlnfptnvdlqrri*rirsincpqkmflnn*n***kcfshkq
iylnv****niiifngc*slsdy

Frame B:
vkmfkl*lsimvlvcvkpvllvmmphvlfshqllvvqdtlvlwsvwvkktlt*vmkpnqr
evs*psntqlntvllptgmiwkksgiilstmnsvllqkntqfs*lkp---

---ERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIVHRKCF*tiktsdesashtnk
yi*mynnnki*lflmvvdlypti

Frame C:
*rcssfsyr*wfwyv*srfcw**cptccfpincwsskthwcygrygskrllrr**spike
rylnpqipn*trycyqlg*ygknlasyflq*tpccsrrtpssln*s---

---nvntlsglvdls*lhsqlsnkcgspkknmtnqvhqlstenvskqlklvmkvlltqtn
ifkciiiikynyf*wllifirls
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO611 (VFO611Q) /CSM/VF/VFO6-A/VFO611Q.Seq.d/                        638   0.0
VFO483 (VFO483Q) /CSM/VF/VFO4-D/VFO483Q.Seq.d/                        638   0.0
VFO120 (VFO120Q) /CSM/VF/VFO1-A/VFO120Q.Seq.d/                        638   0.0
VFN701 (VFN701Q) /CSM/VF/VFN7-A/VFN701Q.Seq.d/                        638   0.0
VFM863 (VFM863Q) /CSM/VF/VFM8-C/VFM863Q.Seq.d/                        638   0.0
VFM807 (VFM807Q) /CSM/VF/VFM8-A/VFM807Q.Seq.d/                        638   0.0
VFM725 (VFM725Q) /CSM/VF/VFM7-B/VFM725Q.Seq.d/                        638   0.0
VFM720 (VFM720Q) /CSM/VF/VFM7-A/VFM720Q.Seq.d/                        638   0.0
VFM705 (VFM705Q) /CSM/VF/VFM7-A/VFM705Q.Seq.d/                        638   0.0
VFM609 (VFM609Q) /CSM/VF/VFM6-A/VFM609Q.Seq.d/                        638   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC116957) Dictyostelium discoideum chromosome 2 map 1685067...   638   0.0    3
(AC116986) Dictyostelium discoideum chromosome 2 map 2234041...   543   0.0    3
(X03281) Dictyostelium discoideum gene for actin A8.              599   0.0    2
(AU284263) Dictyostelium discoideum gamete cDNA clone:FC-AF0...   561   0.0    2
(AU284228) Dictyostelium discoideum gamete cDNA clone:FC-AB0...   539   0.0    2
(M14146) D.discoideum actin 15 gene, complete cds.                555   0.0    4
(AC115680) Dictyostelium discoideum chromosome 2 map 4915084...   555   0.0    4
(DL184438) Protein expression method.                             555   0.0    2
(DL073162) Expression method of recombinant protein having t...   555   0.0    2
(DL184440) Protein expression method.                             555   0.0    2
dna update 2009. 3.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A28258(A28258)actin 5C - fruit fly (Drosophila melanogaster)          236   4e-61
A61043(A61043)actin CA15 - sea squirt (Styela clava) (fragments)      229   3e-59
(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   226   3e-58
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   226   3e-58
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   226   3e-58
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   226   3e-58
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   223   2e-57
AY294152_1(AY294152|pid:none) Mayorella sp. JJP-2003 actin mRNA,...   222   5e-57
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   222   5e-57
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        221   8e-57
protein update 2009. 6. 2
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 24.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for SLD504 is cyt
5' end seq. ID SLD504F
5' end seq. 
>SLD504F.Seq
GGTGAAGATGTTCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGTTTT
GCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACT
GGTGTTATGGTCGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCAAAG
AGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATTGTTACCAACTGGGATGAT
ATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGAAGAACAC
CCAGTTCTCTTAACTGAAGCCC----------
Length of 5' end seq. 322
3' end seq. ID SLD504Z
3' end seq. 
>SLD504Z.Seq
----------AGAACGTAAATACTCTGTCTGGATTGGTGGATCTATCTTAGCTTCACTCT
CAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATATGACGAATCAGGTCCATCAATTG
TCCACAGAAAATGTTTCTAAACAATTAAAACTAGTGATGAAAGTGCTTCTCACACAAACA
AATATATTTAAATGTATAATAATAATAAAATATAATTATTTTTAATGGTTGTTGATCTTT
ATCCGACTATCA
Length of 3' end seq. 242
Connected seq. ID SLD504P
Connected seq. 
>SLD504P.Seq
GGTGAAGATGTTCAAGCTTTAGTTATCGATAATGGTTCTGGTATGTGTAAAGCCGGTTTT
GCTGGTGATGATGCCCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACT
GGTGTTATGGTCGGTATGGGTCAAAAAGACTCTTACGTAGGTGATGAAGCCCAATCAAAG
AGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATTGTTACCAACTGGGATGAT
ATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCTCCAGAAGAACAC
CCAGTTCTCTTAACTGAAGCCC----------AGAACGTAAATACTCTGTCTGGATTGGT
GGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAATAT
GACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTCTAAACAATTAAAACTAGTGAT
GAAAGTGCTTCTCACACAAACAAATATATTTAAATGTATAATAATAATAAAATATAATTA
TTTTTAATGGTTGTTGATCTTTATCCGACTATCA
Length of connected seq. 564
Full length Seq ID -
Full length Seq. -
Length of full length seq. -