NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLD502
Library SL
(Link to library)
Clone ID SLD502
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID SLD502P
(Link to Original site)
Representative DNA sequence 
>SLD502 (SLD502Q) /CSM/SL/SLD5-A/SLD502Q.Seq.d/
AAAAAATAAAAATGGATGGTGAAGATGTTCAAGCTTTAGTTATTGATAACGGTTCTGGTA
TGTGTAAAGCCGGTTTTGCTGGTGACGATGCTCCACGTGCTGTTTTCCCATCAATTGTTG
GTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAAGACTCATATGTAGGTG
ATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATCG
TTACCAACTGGGATGATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCCXXX
XXXXXXXTCTGGATTGGTGGATCTATTTTAGCTTCACTCTCAACTTTCCAACAAATGTGG
ATCTCAAAAGAAGAATATGATGAATCTGGTCCATCAATTGTCCACAGAAAATGTTTCTAA
ATCATGATGAAAGTGCTTCACATAAAAATAATAATAATAATATAACAATAATAATATTTA
AATGTATAATAAAATTTAATTACTTTTTTTTTAATGGTTGTTGATCTTTATCCGACCTTA
AAAAAAAAAAAAT
sequence update 1999. 2.23
Translated Amino Acid sequence 
KIKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDSYVGD
EAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNEL---

---WIGGSILASLSTFQQMWISKEEYDESGPSIVHRKCF*immkvlhikiiiii*q**yl
nv**nlitfflmvvdlyptlkkkk
Translated Amino Acid sequence (All Frames) 
Frame A:
kk*kwmvkmfkl*llitvlvcvkpvllvtmlhvlfshqllvvqdtlvlwlvwvkkthm*v
mkpnqrevs*psntqlntvslptgmiwkksgiilstmns---

---sglvdlf*lhsqlsnkcgsqkknmmnlvhqlstenvsks**kcft*k****ynnnni
*mynki*llff*wllifirp*kkkn

Frame B:
knkngw*rcssfsy**rfwyv*srfcw*rcstccfpincwsskthwcygwygskrlicr*
*spikerylnpqipn*tryryqlg*ygknlasyflq*t---

---ldwwiyfsftlnfptnvdlkrri**iwsincpqkmflnhdesashknnnnnitiiif
kciikfnyfffngc*slsdlkkkk

Frame C:
KIKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDSYVGD
EAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNEL---

---WIGGSILASLSTFQQMWISKEEYDESGPSIVHRKCF*immkvlhikiiiii*q**yl
nv**nlitfflmvvdlyptlkkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLD502 (SLD502Q) /CSM/SL/SLD5-A/SLD502Q.Seq.d/                        791   0.0
VFM527 (VFM527Q) /CSM/VF/VFM5-B/VFM527Q.Seq.d/                        565   e-160
VFL462 (VFL462Q) /CSM/VF/VFL4-C/VFL462Q.Seq.d/                        565   e-160
VFL353 (VFL353Q) /CSM/VF/VFL3-C/VFL353Q.Seq.d/                        565   e-160
VFL311 (VFL311Q) /CSM/VF/VFL3-A/VFL311Q.Seq.d/                        565   e-160
VFK419 (VFK419Q) /CSM/VF/VFK4-A/VFK419Q.Seq.d/                        565   e-160
VFK366 (VFK366Q) /CSM/VF/VFK3-C/VFK366Q.Seq.d/                        565   e-160
VFK110 (VFK110Q) /CSM/VF/VFK1-A/VFK110Q.Seq.d/                        565   e-160
VFJ294 (VFJ294Q) /CSM/VF/VFJ2-D/VFJ294Q.Seq.d/                        565   e-160
VFI680 (VFI680Q) /CSM/VF/VFI6-D/VFI680Q.Seq.d/                        565   e-160
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(M14146) D.discoideum actin 15 gene, complete cds.                565   0.0    3
(AC115680) Dictyostelium discoideum chromosome 2 map 4915084...   565   0.0    3
(DL184438) Protein expression method.                             565   0.0    2
(DL073162) Expression method of recombinant protein having t...   565   0.0    2
(DL184440) Protein expression method.                             565   0.0    2
(DL073163) Expression method of recombinant protein having t...   565   0.0    2
(DL184437) Protein expression method.                             549   0.0    2
(DL184439) Protein expression method.                             549   0.0    2
(DL073160) Expression method of recombinant protein having t...   541   0.0    2
(DL073161) Expression method of recombinant protein having t...   541   0.0    2
dna update 2009. 3.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A28258(A28258)actin 5C - fruit fly (Drosophila melanogaster)          164   2e-56
(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   203   3e-51
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   203   3e-51
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   201   8e-51
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   201   1e-50
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   198   7e-50
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        198   7e-50
AB111103_1(AB111103|pid:none) Candida boidinii ACT1 gene for act...   197   2e-49
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   196   3e-49
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   196   3e-49
protein update 2009. 6. 3
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.14  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SLD502 is cyt
5' end seq. ID SLD502F
5' end seq. 
>SLD502F.Seq
AAAAAATAAAAATGGATGGTGAAGATGTTCAAGCTTTAGTTATTGATAACGGTTCTGGTA
TGTGTAAAGCCGGTTTTGCTGGTGACGATGCTCCACGTGCTGTTTTCCCATCAATTGTTG
GTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAAGACTCATATGTAGGTG
ATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATCG
TTACCAACTGGGATGATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCC---
-------
Length of 5' end seq. 297
3' end seq. ID SLD502Z
3' end seq. 
>SLD502Z.Seq
----------TCTGGATTGGTGGATCTATTTTAGCTTCACTCTCAACTTTCCAACAAATG
TGGATCTCAAAAGAAGAATATGATGAATCTGGTCCATCAATTGTCCACAGAAAATGTTTC
TAAATCATGATGAAAGTGCTTCACATAAAAATAATAATAATAATATAACAATAATAATAT
TTAAATGTATAATAAAATTTAATTACTTTTTTTTTAATGGTTGTTGATCTTTATCCGACC
TTAAAAAAAAAAAAAT
Length of 3' end seq. 246
Connected seq. ID SLD502P
Connected seq. 
>SLD502P.Seq
AAAAAATAAAAATGGATGGTGAAGATGTTCAAGCTTTAGTTATTGATAACGGTTCTGGTA
TGTGTAAAGCCGGTTTTGCTGGTGACGATGCTCCACGTGCTGTTTTCCCATCAATTGTTG
GTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAAGACTCATATGTAGGTG
ATGAAGCCCAATCAAAGAGAGGTATCTTAACCCTCAAATACCCAATTGAACACGGTATCG
TTACCAACTGGGATGATATGGAAAAAATCTGGCATCATACTTTCTACAATGAACTCC---
-------TCTGGATTGGTGGATCTATTTTAGCTTCACTCTCAACTTTCCAACAAATGTGG
ATCTCAAAAGAAGAATATGATGAATCTGGTCCATCAATTGTCCACAGAAAATGTTTCTAA
ATCATGATGAAAGTGCTTCACATAAAAATAATAATAATAATATAACAATAATAATATTTA
AATGTATAATAAAATTTAATTACTTTTTTTTTAATGGTTGTTGATCTTTATCCGACCTTA
AAAAAAAAAAAAT
Length of connected seq. 543
Full length Seq ID -
Full length Seq. -
Length of full length seq. -