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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLD494
Library SL
(Link to library)
Clone ID SLD494
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U00917-1|Contig-U01214-1|Contig-U14915-1|Contig-U15437-1
Original site URL
Representative seq. ID SLD494P
(Link to Original site)
Representative DNA sequence 
>SLD494 (SLD494Q) /CSM/SL/SLD4-D/SLD494Q.Seq.d/
CAATATGGTGCAGAAGGTTTGAAGTTCTTTGAGAACGGAGCATTTGGTGAAGACGCAGCA
TATATTGCAAAGTTGATGGGATCATTGAAATTGATAGCATGTACAGGGTTTTTAATATTA
TTGATAGCGATCCTTTTCCCAATATTCATAGTTGTCAAGACCAAAGGCGCCGTCGATTGG
TCATGGGCAAAAGTATTTTCACCAGCATGGATCATCATGTCATTGGTTATAGTGTTTACX
XXXXXXXXXACATCATTTTCTATTTGGTTTTAATGGCATTAGATAATAGTTCAAAAATGA
ACAATTTATCAATGGATGAAGATGAAAGAGCCTCAAATGAAAGTGTTAATAGTTTCTTAT
ATTGTATTTTCATTTTCTTAATGATTCCAGTTATCTTATTTATTTCTTTATTAGCTGCTC
ATATCTCTGCTGATAGATTTGCAATTGCTAATGTATTCATTCCAATTTTTACTGTTTTAG
GTCTNTTAACTTGTGCATATTGTATTTTTGCATGTTGTGCTTTAGCTGCAGCAAAGGGAC
AAGGAATGGAAGATTTCCCAGGTGGTTCACCAACTCAAAATATCGCTTCAATCATTAACA
GTAGAATGTTAAGACCACAAAAATTCAAGGAAGCTCAACACTTACCAACAACCAGTACCT
CTGTTGGTGTAGAAAATGTTTAATTTTACCAAAATAAAGAGTAAAAAGTTCACATATATA
TAAAATTTTAAAAACTAAAAATAAAAAAAAAAACTAAAAATAAAAATCA
sequence update 1999. 2.23
Translated Amino Acid sequence 
QYGAEGLKFFENGAFGEDAAYIAKLMGSLKLIACTGFLILLIAILFPIFIVVKTKGAVDW
SWAKVFSPAWIIMSLVIVF---

---IIFYLVLMALDNSSKMNNLSMDEDERASNESVNSFLYCIFIFLMIPVILFISLLAAH
ISADRFAIANVFIPIFTVLGLLTCAYCIFACCALAAAKGQGMEDFPGGSPTQNIASIINS
RMLRPQKFKEAQHLPTTSTSVGVENV*fyqnke*kvhiyikf*klkikkktknkn
Translated Amino Acid sequence (All Frames) 
Frame A:
QYGAEGLKFFENGAFGEDAAYIAKLMGSLKLIACTGFLILLIAILFPIFIVVKTKGAVDW
SWAKVFSPAWIIMSLVIVF---

---tsfsiwf*wh*iivqk*tiyqwmkmkepqmkvlivsyivfsfs**fqlsylfly*ll
isllidlqllmysfqfllf*vx*lvhivflhvvl*lqqrdkewkisqvvhqlkislqslt
vec*dhknsrklntyqqpvpllv*kmfnftkikskkftyi*nfkn*k*kkklkiki

Frame B:
nmvqkv*sslrtehlvktqhilqs*wdh*n**hvqgf*yy**rsfsqys*lsrpkapsig
hgqkyfhqhgsschwl*cl---

---hhflfgfngir**fkneqfing*r*kslk*kc**flilyfhflndssyliyffiscs
ylc**icnc*cihsnfycfrsxnlcilyfcmlcfscskgtrngrfprwftnskyrfnh*q
*nvkttkiqgsstltnnqylcwcrkclilpk*rvksshiykilktknkkkn*k*ks

Frame C:
iwcrrfevl*ersiw*rrsiyckvdgiieidsmyrvfniidsdpfpnihscqdqrrrrlv
mgksiftsmdhhvigysvy---

---IIFYLVLMALDNSSKMNNLSMDEDERASNESVNSFLYCIFIFLMIPVILFISLLAAH
ISADRFAIANVFIPIFTVLGLLTCAYCIFACCALAAAKGQGMEDFPGGSPTQNIASIINS
RMLRPQKFKEAQHLPTTSTSVGVENV*fyqnke*kvhiyikf*klkikkktknkn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLD494 (SLD494Q) /CSM/SL/SLD4-D/SLD494Q.Seq.d/                       1267   0.0
SFC702 (SFC702Q) /CSM/SF/SFC7-A/SFC702Q.Seq.d/                        795   0.0
SLC464 (SLC464Q) /CSM/SL/SLC4-C/SLC464Q.Seq.d/                        567   e-161
VHC475 (VHC475Q) /CSM/VH/VHC4-D/VHC475Q.Seq.d/                         38   0.19
VFN152 (VFN152Q) /CSM/VF/VFN1-C/VFN152Q.Seq.d/                         36   0.76
SSL657 (SSL657Q) /CSM/SS/SSL6-C/SSL657Q.Seq.d/                         36   0.76
SSL544 (SSL544Q) /CSM/SS/SSL5-B/SSL544Q.Seq.d/                         36   0.76
SSF536 (SSF536Q) /CSM/SS/SSF5-B/SSF536Q.Seq.d/                         36   0.76
SSA168 (SSA168Q) /CSM/SS/SSA1-C/SSA168Q.Seq.d/                         36   0.76
SLK637 (SLK637Q) /CSM/SL/SLK6-B/SLK637Q.Seq.d/                         36   0.76
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC116920) Dictyostelium discoideum chromosome 2 map 4415041...   474   0.0    3
(AU052540) Dictyostelium discoideum slug cDNA, clone SLD494.      833   0.0    1
(BJ399441) Dictyostelium discoideum cDNA clone:dds6d01, 3' e...   414   0.0    2
(AU034554) Dictyostelium discoideum slug cDNA, clone SLC464.      567   e-157  1
(AU061184) Dictyostelium discoideum slug cDNA, clone SLD494.      474   e-129  1
(AC117332) Rattus norvegicus clone CH230-332H3, *** SEQUENCI...    42   0.013  4
(AQ692682) HS_5415_A2_D09_SP6E RPCI-11 Human Male BAC Librar...    50   0.12   1
(AP001125) Homo sapiens genomic DNA, chromosome 18q12 clone:...    38   0.17   5
(AC128247) Rattus norvegicus clone CH230-523D17, WORKING DRA...    40   0.34   3
(BV648293) S215P60614RF2.T0 Clara Pan troglodytes troglodyte...    48   0.49   1
dna update 2009. 3.17
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3159261
Number of Hits to DB: 1,167,914,267
protein update 2009. 6. 2
PSORT 

 psg: 0.75  gvh: 0.63  alm: 0.10  top: 0.67  tms: 0.27  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 1.00  m_ : 0.00 

	 32.0 %: plasma membrane
	 20.0 %: endoplasmic reticulum
	 12.0 %: nuclear
	 12.0 %: vacuolar
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: cytoplasmic

>> prediction for SLD494 is pla
5' end seq. ID SLD494F
5' end seq. 
>SLD494F.Seq
CAATATGGTGCAGAAGGTTTGAAGTTCTTTGAGAACGGAGCATTTGGTGAAGACGCAGCA
TATATTGCAAAGTTGATGGGATCATTGAAATTGATAGCATGTACAGGGTTTTTAATATTA
TTGATAGCGATCCTTTTCCCAATATTCATAGTTGTCAAGACCAAAGGCGCCGTCGATTGG
TCATGGGCAAAAGTATTTTCACCAGCATGGATCATCATGTCATTGGTTATAGTGTTTAC-
---------
Length of 5' end seq. 239
3' end seq. ID SLD494Z
3' end seq. 
>SLD494Z.Seq
----------ACATCATTTTCTATTTGGTTTTAATGGCATTAGATAATAGTTCAAAAATG
AACAATTTATCAATGGATGAAGATGAAAGAGCCTCAAATGAAAGTGTTAATAGTTTCTTA
TATTGTATTTTCATTTTCTTAATGATTCCAGTTATCTTATTTATTTCTTTATTAGCTGCT
CATATCTCTGCTGATAGATTTGCAATTGCTAATGTATTCATTCCAATTTTTACTGTTTTA
GGTCTNTTAACTTGTGCATATTGTATTTTTGCATGTTGTGCTTTAGCTGCAGCAAAGGGA
CAAGGAATGGAAGATTTCCCAGGTGGTTCACCAACTCAAAATATCGCTTCAATCATTAAC
AGTAGAATGTTAAGACCACAAAAATTCAAGGAAGCTCAACACTTACCAACAACCAGTACC
TCTGTTGGTGTAGAAAATGTTTAATTTTACCAAAATAAAGAGTAAAAAGTTCACATATAT
ATAAAATTTTAAAAACTAAAAATAAAAAAAAAAACTAAAAATAAAAATCA
Length of 3' end seq. 520
Connected seq. ID SLD494P
Connected seq. 
>SLD494P.Seq
CAATATGGTGCAGAAGGTTTGAAGTTCTTTGAGAACGGAGCATTTGGTGAAGACGCAGCA
TATATTGCAAAGTTGATGGGATCATTGAAATTGATAGCATGTACAGGGTTTTTAATATTA
TTGATAGCGATCCTTTTCCCAATATTCATAGTTGTCAAGACCAAAGGCGCCGTCGATTGG
TCATGGGCAAAAGTATTTTCACCAGCATGGATCATCATGTCATTGGTTATAGTGTTTAC-
---------ACATCATTTTCTATTTGGTTTTAATGGCATTAGATAATAGTTCAAAAATGA
ACAATTTATCAATGGATGAAGATGAAAGAGCCTCAAATGAAAGTGTTAATAGTTTCTTAT
ATTGTATTTTCATTTTCTTAATGATTCCAGTTATCTTATTTATTTCTTTATTAGCTGCTC
ATATCTCTGCTGATAGATTTGCAATTGCTAATGTATTCATTCCAATTTTTACTGTTTTAG
GTCTNTTAACTTGTGCATATTGTATTTTTGCATGTTGTGCTTTAGCTGCAGCAAAGGGAC
AAGGAATGGAAGATTTCCCAGGTGGTTCACCAACTCAAAATATCGCTTCAATCATTAACA
GTAGAATGTTAAGACCACAAAAATTCAAGGAAGCTCAACACTTACCAACAACCAGTACCT
CTGTTGGTGTAGAAAATGTTTAATTTTACCAAAATAAAGAGTAAAAAGTTCACATATATA
TAAAATTTTAAAAACTAAAAATAAAAAAAAAAACTAAAAATAAAAATCA
Length of connected seq. 759
Full length Seq ID -
Full length Seq. -
Length of full length seq. -