>AFK261 (AFK261Q) /CSM/AF/AFK2-C/AFK261Q.Seq.d/
XXXXXXXXXXTGATTCAGTTGCCAATAAAGCTCGTNACAATTTCGATGAAAAAGAAACCT
TCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCAT
TTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTA
CCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTACGCTC
CATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATACTTAA
AAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACCAAGTG
ATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAAAATC
CAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCAAGATA
ACGCTGCCATTGAATTCAAATTACCATCATCTCAAACTGATGATAATGTGAATCTAAAAA
AGATTCTTCTGCTAAACA

---DSVANKARXNFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGT
TFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSD
IEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVEFIQDNAAIEFKLPSSQTDDNVNLKK
ILLLN

Frame A:
---DSVANKARXNFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGT
TFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSD
IEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVEFIQDNAAIEFKLPSSQTDDNVNLKK
ILLLN


Frame B:
---iqlpiklvtismkkkpshsipspngskmslvvkflhllnlnqnqnqmmlqlrlplvp
lskswf*thqrmfwlnstlhgaviarillqstin*vnt*ktlnlsqs*rlmptqmmyqvi
sklevtqpscssklmtkkiqslmkvkemii*tllnsfkitlplnsnyhhlklmim*i*kr
ffc*t


Frame C:
---fscq*ssxqfr*krnlliryrlqmdprchww*sfsic*istrtrik*css*gcrwyh
fqkvgfrltkgcfg*ilrsmvrslqescsnlr*ir*ilkrr*iclnrkd*crlk*ctk*y
rn*rlpnhhalqs**qrksnll*rskk*syelc*ihsr*rch*iqitiisn***ceskkd
ssak

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFK816 (VFK816Q) /CSM/VF/VFK8-A/VFK816Q.Seq.d/                       1080   0.0
SFK855 (SFK855Q) /CSM/SF/SFK8-C/SFK855Q.Seq.d/                       1080   0.0
AFO686 (AFO686Q) /CSM/AF/AFO6-D/AFO686Q.Seq.d/                       1080   0.0
AFN855 (AFN855Q) /CSM/AF/AFN8-C/AFN855Q.Seq.d/                       1080   0.0
AFN822 (AFN822Q) /CSM/AF/AFN8-A/AFN822Q.Seq.d/                       1080   0.0
AFN712 (AFN712Q) /CSM/AF/AFN7-A/AFN712Q.Seq.d/                       1080   0.0
AFN679 (AFN679Q) /CSM/AF/AFN6-D/AFN679Q.Seq.d/                       1080   0.0
AFN674 (AFN674Q) /CSM/AF/AFN6-D/AFN674Q.Seq.d/                       1080   0.0
AFN461 (AFN461Q) /CSM/AF/AFN4-C/AFN461Q.Seq.d/                       1080   0.0
AFN443 (AFN443Q) /CSM/AF/AFN4-B/AFN443Q.Seq.d/                       1080   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end, single read.   599  e-167  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74  2e-09  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.    70  3e-08  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70  3e-08  1
BM280519|BM280519.1 ki01h03.y1 Ascaris suum L4 pSPORT1 Zarlenga v1 Ascaris suum cDNA 5' similar to SW:ERP5_CAEEL Q11067 PROBABLE PROTEIN DISULFIDE ISOMERASE P5 PRECURSOR ;, mRNA sequence.    68  1e-07  1
AW255071|AW255071.1 ML1365 peppermint glandular trichome Mentha x piperita cDNA, mRNA sequence.    64  2e-06  1
BM315730|BM315730.1 fw64d09.y1 Sugano SJD adult male Danio rerio cDNA clone 5612584 5' similar to SW:ER60_HUMAN P30101 PROBABLE PROTEIN DISULFIDE ISOMERASE ER-60 PRECURSOR ;, mRNA sequence.    62  8e-06  1
BM308748|BM308748.1 sak49h10.y1 Gm-c1036 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1036-6523 5' similar to TR:P93358 P93358 PROTEIN DISULFIDE-ISOMERASE PRECURSOR. ;, mRNA sequence.    60  3e-05  1
BU795021|S34275 protein disulfide-isomerase homolog precursor - fluke  (Schistosoma mansoni), mRNA sequence.    60  3e-05  1
BM174369|PDA4_MOUSE PROTEIN DISULFIDE ISOMERASE A4 PRECURSOR (PROTEIN ERP-72) (ERP72), mRNA sequence.    60  3e-05  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   168   1e-40
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   161   9e-39
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   157   2e-37
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   154   1e-36
AB022013_1(AB022013|pid:none) Antheraea pernyi mRNA for protein ...   151   9e-36
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   147   1e-34
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   147   2e-34
(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   147   2e-34
BC097044_1(BC097044|pid:none) Danio rerio protein disulfide isom...   147   2e-34
(P13667) RecName: Full=Protein disulfide-isomerase A4;          ...   147   2e-34

 psg: 0.40  gvh: 0.38  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFK261 is nuc

>AFK261Z.Seq
----------TGATTCAGTTGCCAATAAAGCTCGTNACAATTTCGATGAAAAAGAAACCT
TCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCAT
TTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTA
CCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTACGCTC
CATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATACTTAA
AAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACCAAGTG
ATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAAAATC
CAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCAAGATA
ACGCTGCCATTGAATTCAAATTACCATCATCTCAAACTGATGATAATGTGAATCTAAAAA
AGATTCTTCTGCTAAACA