>AFK259 (AFK259Q) /CSM/AF/AFK2-C/AFK259Q.Seq.d/
XXXXXXXXXXGNTCCNCCAAAAACCACCAGGTCCNCCACCACCACCACCAGCTCCAAAAC
CACCAGCCGCAGGTGGCGGTACTGGAAGAAATGCCCTTTTAGGTTCAATTGAAAATTTCT
CAAAGGGTGGACTTAAAAAGACAGTTACAGTTGATAAAAGCGCTGGTGTACCAACAAAAA
CCACACCATCTGCAAATTCAGCTGCCAGCAATGCTGGATCAGAGCCATCATCAGGCGGTT
CAACACCAGCACCAGCTCCAAAATCTAGCGGTGGTGGTGGCGGTGGTGATTTAATGGCCG
AAGTTATGGCCAAGAGAGCCAAAATGAAGGCAGCAGCATCTCAACCAAAAGAAGAATCAT
CAGCACCAACTCCAGCACCAACTCCAGCACCAACTCCAGCACCAACCCCATCATCAACAT
CTTTCAAACCACCACAATCATTCAGTAAACCAGCAACTAAAACAGCTGCTGCAAACAAAC
CACCTTCACCATCCTTATCAGCTCCACTCCCATCAACTGTAGCCAATGAAGATTTACAAT
CACTCAAAGAAGAAATTTTAACTGAAGTTAGAAAGGAAATTCAAAAAGCTAAAGATGAAA
TTTTAGAAGCAATCAGAGCATCTCNNCATTAAATTAATACTTTAA

---XXQKPPGPPPPPPAPKPPAAGGGTGRNALLGSIENFSKGGLKKTVTVDKSAGVPTKT
TPSANSAASNAGSEPSSGGSTPAPAPKSSGGGGGGDLMAEVMAKRAKMKAAASQPKEESS
APTPAPTPAPTPAPTPSSTSFKPPQSFSKPATKTAAANKPPSPSLSAPLPSTVANEDLQS
LKEEILTEVRKEIQKAKDEILEAIRASXH*intl

Frame A:
---XXQKPPGPPPPPPAPKPPAAGGGTGRNALLGSIENFSKGGLKKTVTVDKSAGVPTKT
TPSANSAASNAGSEPSSGGSTPAPAPKSSGGGGGGDLMAEVMAKRAKMKAAASQPKEESS
APTPAPTPAPTPAPTPSSTSFKPPQSFSKPATKTAAANKPPSPSLSAPLPSTVANEDLQS
LKEEILTEVRKEIQKAKDEILEAIRASXH*intl


Frame B:
---sxknhqvxhhhhqlqnhqpqvavleempf*vqlkisqrvdlkrqlqlikalvyqqkp
hhlqiqlpamldqshhqavqhqhqlqnlavvvavvi*wpklwprepk*rqqhlnqkknhq
hqlqhqlqhqlqhqphhqhlsnhhnhsvnqqlkqllqtnhlhhpyqlhshql*pmkiynh
skkkf*lklerkfkklkmkf*kqsehlxiklil*


Frame C:
---ppkttrsxttttsskttsrrwrywkkcpfrfn*kflkgwt*kdsys**krwctnknh
tickfscqqcwiraiirrfntstsski*rwwwrw*fngrsygqesqnegssistkrriis
tnsstnsstnsstnpiinifqtttiiq*tsn*nscckqttftilisstpincsq*rftit
qrrnfn*s*kgnsks*r*nfrsnqsisxln*yf

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLC230 (SLC230Q) /CSM/SL/SLC2-B/SLC230Q.Seq.d/                       1140   0.0
FC-209 (FC-209Q) /CSM/CG/CG-FC/FC-209Q.Seq.d/                        1140   0.0
AFK259 (AFK259Q) /CSM/AF/AFK2-C/AFK259Q.Seq.d/                       1140   0.0
SLH329 (SLH329Q) /CSM/SL/SLH3-B/SLH329Q.Seq.d/                       1132   0.0
AFO838 (AFO838Q) /CSM/AF/AFO8-B/AFO838Q.Seq.d/                       1132   0.0
AFG225 (AFG225Q) /CSM/AF/AFG2-B/AFG225Q.Seq.d/                       1122   0.0
AFM687 (AFM687Q) /CSM/AF/AFM6-D/AFM687Q.Seq.d/                       1094   0.0
SFF608 (SFF608Q) /CSM/SF/SFF6-A/SFF608Q.Seq.d/                       1078   0.0
FC-BD13 (FC-BD13Q) /CSM/FC/FC-BD/FC-BD13Q.Seq.d/                     1078   0.0
FC-AZ18 (FC-AZ18Q) /CSM/FC/FC-AZ/FC-AZ18Q.Seq.d/                     1078   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AL205967|AL205967.1 Tetraodon nigroviridis genome survey sequence T7 end of clone 152K16 of library G from Tetraodon nigroviridis.    56  1e-08  3
CC871017|CC871017.1 NDL.109E4.T7 Notre Dame Liverpool Aedes aegypti genomic clone NotreDame Liverpool-109E4, genomic survey sequence.    62  4e-06  2
CC850783|CC850783.1 NDL.109H18.T7 Notre Dame Liverpool Aedes aegypti genomic clone NotreDame Liverpool-109H18, genomic survey sequence.    62  4e-06  2
CC862537|CC862537.1 NDL.109E5.T7 Notre Dame Liverpool Aedes aegypti genomic clone NotreDame Liverpool-109E5, genomic survey sequence.    62  5e-06  2
AW306469|AW306469.1 se51b09.y1 Gm-c1017 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1017-2418 5', mRNA sequence.    34  8e-04  3
BF008963|BF008963.1 ss71e08.y1 Gm-c1062 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1062-1887 5' similar to TR:Q9S740 Q9S740 F14K14.17 PROTEIN. ;, mRNA sequence.    34  0.001  3
AI899992|AI899992.1 sb97c10.y1 Gm-c1012 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1012-619 5', mRNA sequence.    34  0.002  3
AC120770|AC120770.4 Rattus norvegicus clone CH230-204F23, WORKING DRAFT SEQUENCE, 1 ordered piece.    54  0.002  3
AW394993|AW394993.1 sh38b07.y1 Gm-c1017 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1017-3950 5', mRNA sequence.    34  0.002  3
AQ501049|AQ501049.1 V28E12 mTn-3xHA/lacZ Insertion Library Saccharomyces cerevisiae genomic 5', genomic survey sequence.    34  0.002  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY007501_1(AY007501|pid:none) Hirudo medicinalis enabled-like pr...    42   0.014
X98534_1(X98534|pid:none) H.sapiens VASP gene, exons 4 to 13 and...    40   0.054
(P50552) RecName: Full=Vasodilator-stimulated phosphoprotein;   ...    40   0.054
BC026019_1(BC026019|pid:none) Homo sapiens vasodilator-stimulate...    40   0.054
(P50551) RecName: Full=Vasodilator-stimulated phosphoprotein;   ...    40   0.054
A84509_1(A84509|pid:none) Sequence 124 from Patent WO9845704.  &...    40   0.054
(Q2TA49) RecName: Full=Vasodilator-stimulated phosphoprotein;   ...    40   0.054
(Q5R896) RecName: Full=Ena/VASP-like protein; AltName: Full=Ena/...    39   0.093
(P70460) RecName: Full=Vasodilator-stimulated phosphoprotein;   ...    39   0.16
X98475_1(X98475|pid:none) M.musculus VASP gene.                        39   0.16

 psg: 0.77  gvh: 0.31  alm: 0.57  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 36.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for AFK259 is nuc

>AFK259Z.Seq
----------GNTCCNCCAAAAACCACCAGGTCCNCCACCACCACCACCAGCTCCAAAAC
CACCAGCCGCAGGTGGCGGTACTGGAAGAAATGCCCTTTTAGGTTCAATTGAAAATTTCT
CAAAGGGTGGACTTAAAAAGACAGTTACAGTTGATAAAAGCGCTGGTGTACCAACAAAAA
CCACACCATCTGCAAATTCAGCTGCCAGCAATGCTGGATCAGAGCCATCATCAGGCGGTT
CAACACCAGCACCAGCTCCAAAATCTAGCGGTGGTGGTGGCGGTGGTGATTTAATGGCCG
AAGTTATGGCCAAGAGAGCCAAAATGAAGGCAGCAGCATCTCAACCAAAAGAAGAATCAT
CAGCACCAACTCCAGCACCAACTCCAGCACCAACTCCAGCACCAACCCCATCATCAACAT
CTTTCAAACCACCACAATCATTCAGTAAACCAGCAACTAAAACAGCTGCTGCAAACAAAC
CACCTTCACCATCCTTATCAGCTCCACTCCCATCAACTGTAGCCAATGAAGATTTACAAT
CACTCAAAGAAGAAATTTTAACTGAAGTTAGAAAGGAAATTCAAAAAGCTAAAGATGAAA
TTTTAGAAGCAATCAGAGCATCTCNNCATTAAATTAATACTTTAA