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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFK253
Library AF
(Link to library)
Clone ID AFK253
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFK253P
(Link to Original site)
Representative DNA sequence 
>AFK253 (AFK253Q) /CSM/AF/AFK2-C/AFK253Q.Seq.d/
TATATTTAATAAACTAATAGTTTAAAACTAAAATATCAAGATGCATGTGTTAAATTTATT
ATTTTTAATAGTAATAATTAATATTTTAATTTTTTCTGTACAATCATATACCTTGAAAAA
GTATGATTATGGTCAGTTTTTAGTTATCGCCAGTGGGCCAGTAATTAATAAAAAATTTAA
TGTTGCAAATCACCAGGTATCGAACTTTTTACTAACAAAAGATGAAAATGATTCTAATTC
AGTGCAGCTTCGTATCACTTCTGATGGAGCACCAGAATTTTTAATCTTTTACAGTACTTT
TAGTGATGAAATTGCAAGTGAAAAAAATTCAAACAAAACAAATATTACCACTCCATTGGA
AGGCTCAATGTGTATCTCTAGTGTCTATTCTTTTTCAATCAAAATGAACAATTACCTCTT
GGGTGGTGATTACATTATCTCAATTCTAGGTAACTCTGATGTTTCAAAATGCTACAAGCA
TGTCCCAGTAAACCAAGCTAACCAAGAACCTGAAAGTATTGTATTACTTTCAACATCTTC
TGAACAACTTGTTTTACAATCTGATGGTGAAACAGTCGTTCAAAAAAAAAAATNNAAAAN
TXXXXXXXXXXAAGTATGNNTANGGTCAGTTTTTAGTTATNGCCAGTGGGCCAGTNATTA
ATAAANAATTTAATGTTGCNAATCNCCAGGTATCNNNCTTTTTNCTAACAAAAGATGAAA
ATGATTCTAATTCAGTGCAGCTTTGTNTCACTTCTGATGGAGCNCCAGAATTTTTAATNT
TTTACAGTACTTTTAGTGATGNAATTGCAAGTGAAAAAAATTCNAACNAAACAAATATTA
CCNCTCCATTGGAAGGCNCAATGTGTATCTCTAGTGTCTATTCTTTTTCAATCAAAATGA
ACAATTACCTCTTGGGTGGTGATTACNTTATNTCNATTNTAGGTAACTNTGATGTTTCAA
AATGCTNCAAGCATGTCCCAGTAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ifnkliv*n*NIKMHVLNLLFLIVIINILIFSVQSYTLKKYDYGQFLVIASGPVINKKFN
VANHQVSNFLLTKDENDSNSVQLRITSDGAPEFLIFYSTFSDEIASEKNSNKTNITTPLE
GSMCISSVYSFSIKMNNYLLGGDYIISILGNSDVSKCYKHVPVNQANQEPESIVLLSTSS
EQLVLQSDGETVVQKKKXKX---

---KYXXGQFLVXASGPVINKXFNVANXQVSXFXLTKDENDSNSVQLCXTSDGAPEFLXF
YSTFSDXIASEKNSNXTNITXPLEGXMCISSVYSFSIKMNNYLLGGDYXXSIXGNXDVSK
CXKHVPV
Translated Amino Acid sequence (All Frames) 
Frame A:
yi**tnslklkyqdacvkfiifnsnn*yfnffctiiylekv*lwsvfsyrqwasn**ki*
cckspgielftnkr*k*f*fsaasyhf*wstrifnllqyf***nck*kkfkqnkyyhsig
rlnvyl*clfffnqneqlplgw*lhylnsr*l*cfkmlqacpskps*prt*kycitfnif
*ttcfti*w*nsrskkkxxx---

---KYXXGQFLVXASGPVINKXFNVANXQVSXFXLTKDENDSNSVQLCXTSDGAPEFLXF
YSTFSDXIASEKNSNXTNITXPLEGXMCISSVYSFSIKMNNYLLGGDYXXSIXGNXDVSK
CXKHVPV

Frame B:
ifnkliv*n*NIKMHVLNLLFLIVIINILIFSVQSYTLKKYDYGQFLVIASGPVINKKFN
VANHQVSNFLLTKDENDSNSVQLRITSDGAPEFLIFYSTFSDEIASEKNSNKTNITTPLE
GSMCISSVYSFSIKMNNYLLGGDYIISILGNSDVSKCYKHVPVNQANQEPESIVLLSTSS
EQLVLQSDGETVVQKKKXKX---

---smxxvsf*lxpvgqxlixnlmlxixryxxfx*qkmkmiliqcsfvsllmexqnf*xf
tvllvmxlqvkkixtkqilplhwkaqcvslvsilfqsk*titswvvitlxxx*vtxmfqn
axsmsq*

Frame C:
ylin**fktkisrcmc*iyyf****lif*fflynhip*ksmimvsf*lspvgq*liknlm
lqitryrtfy*qkmkmiliqcsfvsllmehqnf*sftvllvmklqvkkiqtkqilplhwk
aqcvslvsilfqsk*titswvvitlsqf*vtlmfqnatsmsq*tkltknlkvlyyfqhll
nnlfynlmvkqsfkkknxk---

---vxxxsvfsyxqwasx**xi*ccxspgixlfxnkr*k*f*fsaalxhf*wsxrifnxl
qyf**xnck*kkfxxnkyyxsigrxnvyl*clfffnqneqlplgw*lxyxxxr*l*cfkm
lqacpsk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK253 (AFK253Q) /CSM/AF/AFK2-C/AFK253Q.Seq.d/                        922   0.0
SFJ286 (SFJ286Q) /CSM/SF/SFJ2-D/SFJ286Q.Seq.d/                        914   0.0
SFC324 (SFC324Q) /CSM/SF/SFC3-A/SFC324Q.Seq.d/                        914   0.0
CHM774 (CHM774Q) /CSM/CH/CHM7-D/CHM774Q.Seq.d/                        914   0.0
CFG281 (CFG281Q) /CSM/CF/CFG2-D/CFG281Q.Seq.d/                        914   0.0
AHQ785 (AHQ785Q) /CSM/AH/AHQ7-D/AHQ785Q.Seq.d/                        914   0.0
AHQ425 (AHQ425Q) /CSM/AH/AHQ4-B/AHQ425Q.Seq.d/                        914   0.0
AHM641 (AHM641Q) /CSM/AH/AHM6-B/AHM641Q.Seq.d/                        914   0.0
AHA384 (AHA384Q) /CSM/AH/AHA3-D/AHA384Q.Seq.d/                        914   0.0
AFB331 (AFB331Q) /CSM/AF/AFB3-B/AFB331Q.Seq.d/                        914   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BE580766|BE580766.1 kp81d04.y2 TBN95TM-SSFH Strongyloides stercoralis cDNA 5' similar to WP:C18E9.2 CE05294 DROSOPHILA TRANSLOCATION PROTEIN 1 LIKE ;, mRNA sequence.    32  0.16  3
BH515899|BH515899.1 BOGPQ37TR BOGP Brassica oleracea genomic clone BOGPQ37, DNA sequence.    40  1.2  2
BH947847|BH947847.1 obu93b02.b1 B.oleracea002 Brassica oleracea genomic, DNA sequence.    36  1.2  2
BH707470|BH707470.1 BOMJM43TF BO_2_3_KB Brassica oleracea genomic clone BOMJM43, DNA sequence.    40  1.4  2
AC099426|AC099426.7 Rattus norvegicus clone CH230-209O11, WORKING DRAFT SEQUENCE, 6 unordered pieces.    44  3.3  1
BH471876|BH471876.1 BOHAN79TR BOHA Brassica oleracea genomic clone BOHAN79, DNA sequence.    44  3.3  1
BH498349|BH498349.1 BOHFF52TR BOHF Brassica oleracea genomic clone BOHFF52, DNA sequence.    44  3.3  1
AL136059|AL136059.3 Human chromosome 5 DNA sequence BAC R-391B7 of library RPCI-11 from chromosome 5 of Homo sapiens (Human).    40  3.4  2
BX569782|BX569782.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-194H13.    44  4.7  3
CB308873|CB308873.1 AGENCOURT_11774586 NICHD_Rh_Ov1 Macaca mulatta cDNA clone IMAGE:6885562 5', mRNA sequence.    32  4.8  3
dna update 2003. 9. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q11121) RecName: Full=Lysophospholipase;          EC=3.1.1.5; A...    37   0.76
AM114193_2667(AM114193|pid:none) Uncultured methanogenic archaeo...    35   2.9
AL033502_6(AL033502|pid:none) C.albicans cosmid Ca38F10.               35   2.9
AB1261(AB1261) shikimate 5-dehydrogenase (AroD) homolog lmo1490 ...    35   3.8
CP000851_367(CP000851|pid:none) Shewanella pealeana ATCC 700345,...    35   3.8
AP002071_19(AP002071|pid:none) Oryza sativa Japonica Group genom...    34   6.4
CR382126_749(CR382126|pid:none) Kluyveromyces lactis strain NRRL...    34   6.4
AY093107_1(AY093107|pid:none) Arabidopsis thaliana unknown prote...    34   8.4
protein update 2008.11.25
PSORT 

 psg: 1.00  gvh: 0.56  alm: 0.16  top: 0.43  tms: 0.07  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: endoplasmic reticulum
	 16.0 %: cytoplasmic
	 16.0 %: mitochondrial
	 12.0 %: nuclear
	  8.0 %: Golgi
	  8.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: extracellular, including cell wall

>> prediction for AFK253 is end
5' end seq. ID AFK253F
5' end seq. 
>AFK253F.Seq
TATATTTAATAAACTAATAGTTTAAAACTAAAATATCAAGATGCATGTGTTAAATTTATT
ATTTTTAATAGTAATAATTAATATTTTAATTTTTTCTGTACAATCATATACCTTGAAAAA
GTATGATTATGGTCAGTTTTTAGTTATCGCCAGTGGGCCAGTAATTAATAAAAAATTTAA
TGTTGCAAATCACCAGGTATCGAACTTTTTACTAACAAAAGATGAAAATGATTCTAATTC
AGTGCAGCTTCGTATCACTTCTGATGGAGCACCAGAATTTTTAATCTTTTACAGTACTTT
TAGTGATGAAATTGCAAGTGAAAAAAATTCAAACAAAACAAATATTACCACTCCATTGGA
AGGCTCAATGTGTATCTCTAGTGTCTATTCTTTTTCAATCAAAATGAACAATTACCTCTT
GGGTGGTGATTACATTATCTCAATTCTAGGTAACTCTGATGTTTCAAAATGCTACAAGCA
TGTCCCAGTAAACCAAGCTAACCAAGAACCTGAAAGTATTGTATTACTTTCAACATCTTC
TGAACAACTTGTTTTACAATCTGATGGTGAAACAGTCGTTCAAAAAAAAAAATNNAAAAN
T----------
Length of 5' end seq. 601
3' end seq. ID AFK253Z
3' end seq. 
>AFK253Z.Seq
----------AAGTATGNNTANGGTCAGTTTTTAGTTATNGCCAGTGGGCCAGTNATTAA
TAAANAATTTAATGTTGCNAATCNCCAGGTATCNNNCTTTTTNCTAACAAAAGATGAAAA
TGATTCTAATTCAGTGCAGCTTTGTNTCACTTCTGATGGAGCNCCAGAATTTTTAATNTT
TTACAGTACTTTTAGTGATGNAATTGCAAGTGAAAAAAATTCNAACNAAACAAATATTAC
CNCTCCATTGGAAGGCNCAATGTGTATCTCTAGTGTCTATTCTTTTTCAATCAAAATGAA
CAATTACCTCTTGGGTGGTGATTACNTTATNTCNATTNTAGGTAACTNTGATGTTTCAAA
ATGCTNCAAGCATGTCCCAGTAAA
Length of 3' end seq. 374
Connected seq. ID AFK253P
Connected seq. 
>AFK253P.Seq
TATATTTAATAAACTAATAGTTTAAAACTAAAATATCAAGATGCATGTGTTAAATTTATT
ATTTTTAATAGTAATAATTAATATTTTAATTTTTTCTGTACAATCATATACCTTGAAAAA
GTATGATTATGGTCAGTTTTTAGTTATCGCCAGTGGGCCAGTAATTAATAAAAAATTTAA
TGTTGCAAATCACCAGGTATCGAACTTTTTACTAACAAAAGATGAAAATGATTCTAATTC
AGTGCAGCTTCGTATCACTTCTGATGGAGCACCAGAATTTTTAATCTTTTACAGTACTTT
TAGTGATGAAATTGCAAGTGAAAAAAATTCAAACAAAACAAATATTACCACTCCATTGGA
AGGCTCAATGTGTATCTCTAGTGTCTATTCTTTTTCAATCAAAATGAACAATTACCTCTT
GGGTGGTGATTACATTATCTCAATTCTAGGTAACTCTGATGTTTCAAAATGCTACAAGCA
TGTCCCAGTAAACCAAGCTAACCAAGAACCTGAAAGTATTGTATTACTTTCAACATCTTC
TGAACAACTTGTTTTACAATCTGATGGTGAAACAGTCGTTCAAAAAAAAAAATNNAAAAN
T----------AAGTATGNNTANGGTCAGTTTTTAGTTATNGCCAGTGGGCCAGTNATTA
ATAAANAATTTAATGTTGCNAATCNCCAGGTATCNNNCTTTTTNCTAACAAAAGATGAAA
ATGATTCTAATTCAGTGCAGCTTTGTNTCACTTCTGATGGAGCNCCAGAATTTTTAATNT
TTTACAGTACTTTTAGTGATGNAATTGCAAGTGAAAAAAATTCNAACNAAACAAATATTA
CCNCTCCATTGGAAGGCNCAATGTGTATCTCTAGTGTCTATTCTTTTTCAATCAAAATGA
ACAATTACCTCTTGGGTGGTGATTACNTTATNTCNATTNTAGGTAACTNTGATGTTTCAA
AATGCTNCAAGCATGTCCCAGTAAA
Length of connected seq. 975
Full length Seq ID -
Full length Seq. -
Length of full length seq. -