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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLD233
Library SL
(Link to library)
Clone ID SLD233
Atlas ID
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15622-1
Original site URL
Representative seq. ID SLD233P
(Link to Original site)
Representative DNA sequence 
>SLD233 (SLD233Q) /CSM/SL/SLD2-B/SLD233Q.Seq.d/
GAAAGTCAAGCATTTCATTTAGATCAAAATAATACATTATTTCAATTATTTTCACCAGAT
TCAATCGATCCAACAGAGGAGCAAGCAAAGATTGCAACACGTTTAGTATCGTTATGTGTA
TCATTGAATGAATGTCCAATCATTCGTTTCTCACGTAGTCATCCAGTATCAGCAATGATT
GCAGGTTTTACACAAGAGAAATTGGATAATGTTATGAGAACGGTCAAATCATTCAAACCA
AACGATGACCGTTCAACATTATTAATTTTAGATCGTACTCAAGATCCATTAGCACCXXXX
XXXXXXTAATGTACCTTATGAGGTATCTCGTTACGTTCCTGTAGTAAAGGATATCGCTGA
GAATATTATCAATGAAACTTTACCAAGCACAGATTTCCCATTCGTAAAAGAGGAACCAAT
CGCTCGTGCCACAAATGCACCAGTCTCAAAGGTTTCCCTTAAAGGTAAATCAAAACAACC
ACGTTGGGCTGACCCAAATGTACAAGTTGAAGAAACTAAATACTCTGGTTCCAAATTAAT
CATTTTCGTCATTGGTGGTATGACTTTTTCTGAAATGAGATCAATCTATGAATTATCTTC
TCATTATAAAAAGAATATTTATATTGGTTCTACAAATATTTTATTACCAAAGAAATATAT
TGATCAATTATTAACTTTAAAAAATGAAT
sequence update 1999. 2.23
Translated Amino Acid sequence 
ESQAFHLDQNNTLFQLFSPDSIDPTEEQAKIATRLVSLCVSLNECPIIRFSRSHPVSAMI
AGFTQEKLDNVMRTVKSFKPNDDRSTLLILDRTQDPLA---

---NVPYEVSRYVPVVKDIAENIINETLPSTDFPFVKEEPIARATNAPVSKVSLKGKSKQ
PRWADPNVQVEETKYSGSKLIIFVIGGMTFSEMRSIYELSSHYKKNIYIGSTNILLPKKY
IDQLLTLKNE
Translated Amino Acid sequence (All Frames) 
Frame A:
ESQAFHLDQNNTLFQLFSPDSIDPTEEQAKIATRLVSLCVSLNECPIIRFSRSHPVSAMI
AGFTQEKLDNVMRTVKSFKPNDDRSTLLILDRTQDPLA---

---*ctl*gislrscskgyr*eyyq*nftkhrfpirkrgtnrschkctslkgfp*r*ikt
ttlg*pkcts*rn*ilwfqinhfrhwwydff*neinl*iifsl*keylywfykyfitkei
y*siinfkk*

Frame B:
kvkhfi*ikiihyfnyfhqiqsiqqrskqrlqhv*yryvyh*mnvqsfvshvviqyqq*l
qvlhkrnwiml*ersnhsnqtmtvqhy*f*ivlkih*h---

---NVPYEVSRYVPVVKDIAENIINETLPSTDFPFVKEEPIARATNAPVSKVSLKGKSKQ
PRWADPNVQVEETKYSGSKLIIFVIGGMTFSEMRSIYELSSHYKKNIYIGSTNILLPKKY
IDQLLTLKNE

Frame C:
kssisfrsk*yiisiiftrfnrsnrgaskdcntfsivmciie*msnhsflt*sssisndc
rfytreig*cyengqiiqtkr*pfniinfrsysrsist---

---mylmrylvtfl**rislrilsmklyqaqishs*krnqslvpqmhqsqrfplkvnqnn
hvgltqmyklkklntlvpn*sfsslvv*lflk*dqsmnylliikrifilvlqifyyqrni
liny*l*kmn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLD233 (SLD233Q) /CSM/SL/SLD2-B/SLD233Q.Seq.d/                       1306   0.0
SSM460 (SSM460Q) /CSM/SS/SSM4-C/SSM460Q.Seq.d/                        759   0.0
CFH310 (CFH310Q) /CSM/CF/CFH3-A/CFH310Q.Seq.d/                        674   0.0
CHE855 (CHE855Q) /CSM/CH/CHE8-C/CHE855Q.Seq.d/                        333   2e-90
CFJ307 (CFJ307Q) /CSM/CF/CFJ3-A/CFJ307Q.Seq.d/                        268   1e-70
CFH256 (CFH256Q) /CSM/CF/CFH2-C/CFH256Q.Seq.d/                        264   2e-69
AFJ148 (AFJ148Q) /CSM/AF/AFJ1-B/AFJ148Q.Seq.d/                        149   7e-35
VFL712 (VFL712Q) /CSM/VF/VFL7-A/VFL712Q.Seq.d/                         40   0.044
SSL122 (SSL122Q) /CSM/SS/SSL1-A/SSL122Q.Seq.d/                         40   0.044
SSH205 (SSH205Q) /CSM/SS/SSH2-A/SSH205Q.Seq.d/                         40   0.044
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU052386) Dictyostelium discoideum slug cDNA, clone SLD233.      759   0.0    1
(AU039161) Dictyostelium discoideum slug cDNA, clone SSM460.      759   0.0    1
(BJ372931) Dictyostelium discoideum cDNA clone:ddc15d03, 3' ...   630   0.0    2
(AU061109) Dictyostelium discoideum slug cDNA, clone SLD233.      587   e-163  1
(BJ378361) Dictyostelium discoideum cDNA clone:ddc31n14, 3' ...   333   5e-87  1
(BJ361877) Dictyostelium discoideum cDNA clone:ddc19n01, 5' ...   268   3e-67  1
(BJ359632) Dictyostelium discoideum cDNA clone:ddc15o14, 5' ...   264   4e-66  1
(BJ375567) Dictyostelium discoideum cDNA clone:ddc19n01, 3' ...   208   2e-49  1
(BJ359486) Dictyostelium discoideum cDNA clone:ddc15d03, 5' ...   174   3e-39  1
(BJ344477) Dictyostelium discoideum cDNA clone:dda19o11, 3' ...   149   2e-31  1
dna update 2009. 3.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54QC8) RecName: Full=Protein transport protein sec1;                254   2e-66
AB168798_1(AB168798|pid:none) Macaca fascicularis testis cDNA, c...    72   2e-16
(O94590) RecName: Full=Protein transport protein sec1;  &AL03282...    84   4e-15
CR382131_903(CR382131|pid:none) Yarrowia lipolytica strain CLIB1...    80   4e-14
BC133423_1(BC133423|pid:none) Bos taurus syntaxin binding protei...    80   7e-14
(P34815) RecName: Full=Putative acetylcholine regulator unc-18; ...    79   1e-13
D63506_1(D63506|pid:none) Homo sapiens mRNA for unc-18homologue,...    79   1e-13
S66176_1(S66176|pid:none) unc-18=vesicle transport protein [Caen...    79   1e-13
AK223170_1(AK223170|pid:none) Homo sapiens mRNA for syntaxin bin...    79   1e-13
(O00186) RecName: Full=Syntaxin-binding protein 3; AltName: Full...    79   1e-13
protein update 2009. 6. 2
PSORT 

 psg: 0.75  gvh: 0.45  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.12  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SLD233 is cyt
5' end seq. ID SLD233F
5' end seq. 
>SLD233F.Seq
GAAAGTCAAGCATTTCATTTAGATCAAAATAATACATTATTTCAATTATTTTCACCAGAT
TCAATCGATCCAACAGAGGAGCAAGCAAAGATTGCAACACGTTTAGTATCGTTATGTGTA
TCATTGAATGAATGTCCAATCATTCGTTTCTCACGTAGTCATCCAGTATCAGCAATGATT
GCAGGTTTTACACAAGAGAAATTGGATAATGTTATGAGAACGGTCAAATCATTCAAACCA
AACGATGACCGTTCAACATTATTAATTTTAGATCGTACTCAAGATCCATTAGCACC----
------
Length of 5' end seq. 296
3' end seq. ID SLD233Z
3' end seq. 
>SLD233Z.Seq
----------TAATGTACCTTATGAGGTATCTCGTTACGTTCCTGTAGTAAAGGATATCG
CTGAGAATATTATCAATGAAACTTTACCAAGCACAGATTTCCCATTCGTAAAAGAGGAAC
CAATCGCTCGTGCCACAAATGCACCAGTCTCAAAGGTTTCCCTTAAAGGTAAATCAAAAC
AACCACGTTGGGCTGACCCAAATGTACAAGTTGAAGAAACTAAATACTCTGGTTCCAAAT
TAATCATTTTCGTCATTGGTGGTATGACTTTTTCTGAAATGAGATCAATCTATGAATTAT
CTTCTCATTATAAAAAGAATATTTATATTGGTTCTACAAATATTTTATTACCAAAGAAAT
ATATTGATCAATTATTAACTTTAAAAAATGAAT
Length of 3' end seq. 383
Connected seq. ID SLD233P
Connected seq. 
>SLD233P.Seq
GAAAGTCAAGCATTTCATTTAGATCAAAATAATACATTATTTCAATTATTTTCACCAGAT
TCAATCGATCCAACAGAGGAGCAAGCAAAGATTGCAACACGTTTAGTATCGTTATGTGTA
TCATTGAATGAATGTCCAATCATTCGTTTCTCACGTAGTCATCCAGTATCAGCAATGATT
GCAGGTTTTACACAAGAGAAATTGGATAATGTTATGAGAACGGTCAAATCATTCAAACCA
AACGATGACCGTTCAACATTATTAATTTTAGATCGTACTCAAGATCCATTAGCACC----
------TAATGTACCTTATGAGGTATCTCGTTACGTTCCTGTAGTAAAGGATATCGCTGA
GAATATTATCAATGAAACTTTACCAAGCACAGATTTCCCATTCGTAAAAGAGGAACCAAT
CGCTCGTGCCACAAATGCACCAGTCTCAAAGGTTTCCCTTAAAGGTAAATCAAAACAACC
ACGTTGGGCTGACCCAAATGTACAAGTTGAAGAAACTAAATACTCTGGTTCCAAATTAAT
CATTTTCGTCATTGGTGGTATGACTTTTTCTGAAATGAGATCAATCTATGAATTATCTTC
TCATTATAAAAAGAATATTTATATTGGTTCTACAAATATTTTATTACCAAAGAAATATAT
TGATCAATTATTAACTTTAAAAAATGAAT
Length of connected seq. 679
Full length Seq ID -
Full length Seq. -
Length of full length seq. -