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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLD224
Library SL
(Link to library)
Clone ID SLD224
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U01201-1
Original site URL
Representative seq. ID SLD224P
(Link to Original site)
Representative DNA sequence 
>SLD224 (SLD224Q) /CSM/SL/SLD2-A/SLD224Q.Seq.d/
CTTCTACATCAACTACTACAACAAAGATAAATGGCACCTCTAGTAGTAGTAGTAATAGTG
GTAGCAGTAGTATTAGTAAAAAAGCAGAAAAAGAATTAAAGGAACAAGAGAAACAAAAAA
TAAAAGAGTTGATGGATACCTTAAAATCAAAACAATTAACACCAGATCAAGAAAAAGCAA
TTAAATCACAAATTAAGCAACTCAAGAAAGAAAAAAGACAAAAGAAAGAAGAAAAGAAAT
TAGCAAAAAGATTAAAAGAAAAAGAAAAAGAAAGTAAATCAAAATCATCAACATCAACAT
CATCATCTACXXXXXXXXXXTAATAATACCGAATGTAATAATAGTAAAATAAATGATGAA
TCATCAACTAACACCCCAACCACCTCAACCACCACAGCAACAAAGAAGAAAGGAGTAAAT
CATAATGTTGGACATATTATATCACTATGTATTGAAATTACCCATATCGTTAACAAGAGT
AAAGGTCAATTGCTTAGAGATATTGTTGATGAATGTTGCCTATTCCAAGGTCAATGGGAT
GAAGTTGTTGTACCAAAATTACGTCAAGAAATATCATTTAGAAATATCAAACCAATTGGT
ACCGTTTCAATGCGTAAAGGTAGAAAGGGTGCTCCACCAACTCCATTTGCAAATGTTGAA
TCTTTCCAAAATATGGCAAATGGTGTTCCAATTCCTCCAACAAATCCTTCCTCAAAATTC
TTTTTCTAAAAATATAATAATAATAATAATATAATAAAAAAACAAAACAAAAAACAAAAA
CAAAAATATAATAAACAAAAAAGA
sequence update 1999. 2.23
Translated Amino Acid sequence 
STSTTTTKINGTSSSSSNSGSSSISKKAEKELKEQEKQKIKELMDTLKSKQLTPDQEKAI
KSQIKQLKKEKRQKKEEKKLAKRLKEKEKESKSKSSTSTSSS---

---NNTECNNSKINDESSTNTPTTSTTTATKKKGVNHNVGHIISLCIEITHIVNKSKGQL
LRDIVDECCLFQGQWDEVVVPKLRQEISFRNIKPIGTVSMRKGRKGAPPTPFANVESFQN
MANGVPIPPTNPSSKFFF*kynnnnniikkqnkkqkqkynkqkr
Translated Amino Acid sequence (All Frames) 
Frame A:
llhqllqqr*maplvvvvivvavvlvkkqkkn*rnkrnkk*ks*wip*nqnn*hqikkkq
lnhklsnsrkkkdkrkkkrn*qkd*kkkkkkvnqnhqhqhhhl---

---**yrm****nk**iin*hpnhlnhhsnkeersks*cwtyyitmy*nypyr*qe*rsi
a*ryc**mlpiprsmg*scctkitsrnii*kyqtnwyrfna*r*kgcstnsickc*ifpk
ygkwcsnssnksflkilflki*****ynkktkqktktki**tkk

Frame B:
fyinyynkdkwhl******w*q*y**ksrkrikgtretknkrvdgylkiktintrsrksn
*itn*atqerkktkerrkeiskkikrkrkrk*ikiininiiiy---

---NNTECNNSKINDESSTNTPTTSTTTATKKKGVNHNVGHIISLCIEITHIVNKSKGQL
LRDIVDECCLFQGQWDEVVVPKLRQEISFRNIKPIGTVSMRKGRKGAPPTPFANVESFQN
MANGVPIPPTNPSSKFFF*kynnnnniikkqnkkqkqkynkqkr

Frame C:
STSTTTTKINGTSSSSSNSGSSSISKKAEKELKEQEKQKIKELMDTLKSKQLTPDQEKAI
KSQIKQLKKEKRQKKEEKKLAKRLKEKEKESKSKSSTSTSSS---

---iipnviivk*mmnhqltpqppqppqqqrrke*iimldilyhyvlklpisltrvkvnc
leillmnvayskvngmkllyqnyvkkyhleisnqlvpfqcvkvervlhqlhlqmlnlski
wqmvfqflqqilpqnsfskniiiiii**knktknknkniinkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLD224 (SLD224Q) /CSM/SL/SLD2-A/SLD224Q.Seq.d/                        805   0.0
AFN246 (AFN246Q) /CSM/AF/AFN2-B/AFN246Q.Seq.d/                        805   0.0
SLE546 (SLE546Q) /CSM/SL/SLE5-B/SLE546Q.Seq.d/                        787   0.0
VSI481 (VSI481Q) /CSM/VS/VSI4-D/VSI481Q.Seq.d/                         40   0.051
SSG163 (SSG163Q) /CSM/SS/SSG1-C/SSG163Q.Seq.d/                         40   0.051
CHB683 (CHB683Q) /CSM/CH/CHB6-D/CHB683Q.Seq.d/                         40   0.051
CFA769 (CFA769Q) /CSM/CF/CFA7-C/CFA769Q.Seq.d/                         40   0.051
SLJ581 (SLJ581Q) /CSM/SL/SLJ5-D/SLJ581Q.Seq.d/                         38   0.20
SFK686 (SFK686Q) /CSM/SF/SFK6-D/SFK686Q.Seq.d/                         38   0.20
SFG710 (SFG710Q) /CSM/SF/SFG7-A/SFG710Q.Seq.d/                         38   0.20
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC117072) Dictyostelium discoideum chromosome 2 map 3879572...   805   0.0    3
(AU052380) Dictyostelium discoideum slug cDNA, clone SLD224.      805   0.0    1
(BJ347538) Dictyostelium discoideum cDNA clone:dda27k12, 3' ...   805   0.0    1
(AU034822) Dictyostelium discoideum slug cDNA, clone SLE546.      787   0.0    1
(AU061107) Dictyostelium discoideum slug cDNA, clone SLD224.       80   3e-27  2
(BJ330102) Dictyostelium discoideum cDNA clone:dda27k12, 5' ...    80   6e-27  2
(DY588947) GUTF074981O3 POSSUM_01-POSSUM-GUT-2KB Trichosurus...    50   0.13   1
(AM488785) Vitis vinifera contig VV78X107865.13, whole genom...    48   0.51   1
(AM482646) Vitis vinifera contig VV78X265097.6, whole genome...    48   0.51   1
(AM480616) Vitis vinifera contig VV78X134656.4, whole genome...    48   0.51   1
dna update 2009. 3.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117072_48(AC117072|pid:none) Dictyostelium discoideum chromoso...   242   1e-62
CR626927_2782(CR626927|pid:none) Bacteroides fragilis NCTC 9343,...    35   2.8
CP001393_53(CP001393|pid:none) Anaerocellum thermophilum DSM 672...    35   3.6
protein update 2009. 6. 2
PSORT 

 psg: 0.78  gvh: 0.44  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.46  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 68.0 %: mitochondrial
	 16.0 %: cytoplasmic
	  8.0 %: nuclear
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for SLD224 is mit
5' end seq. ID SLD224F
5' end seq. 
>SLD224F.Seq
CTTCTACATCAACTACTACAACAAAGATAAATGGCACCTCTAGTAGTAGTAGTAATAGTG
GTAGCAGTAGTATTAGTAAAAAAGCAGAAAAAGAATTAAAGGAACAAGAGAAACAAAAAA
TAAAAGAGTTGATGGATACCTTAAAATCAAAACAATTAACACCAGATCAAGAAAAAGCAA
TTAAATCACAAATTAAGCAACTCAAGAAAGAAAAAAGACAAAAGAAAGAAGAAAAGAAAT
TAGCAAAAAGATTAAAAGAAAAAGAAAAAGAAAGTAAATCAAAATCATCAACATCAACAT
CATCATCTAC----------
Length of 5' end seq. 310
3' end seq. ID SLD224Z
3' end seq. 
>SLD224Z.Seq
----------TAATAATACCGAATGTAATAATAGTAAAATAAATGATGAATCATCAACTA
ACACCCCAACCACCTCAACCACCACAGCAACAAAGAAGAAAGGAGTAAATCATAATGTTG
GACATATTATATCACTATGTATTGAAATTACCCATATCGTTAACAAGAGTAAAGGTCAAT
TGCTTAGAGATATTGTTGATGAATGTTGCCTATTCCAAGGTCAATGGGATGAAGTTGTTG
TACCAAAATTACGTCAAGAAATATCATTTAGAAATATCAAACCAATTGGTACCGTTTCAA
TGCGTAAAGGTAGAAAGGGTGCTCCACCAACTCCATTTGCAAATGTTGAATCTTTCCAAA
ATATGGCAAATGGTGTTCCAATTCCTCCAACAAATCCTTCCTCAAAATTCTTTTTCTAAA
AATATAATAATAATAATAATATAATAAAAAAACAAAACAAAAAACAAAAACAAAAATATA
ATAAACAAAAAAGA
Length of 3' end seq. 484
Connected seq. ID SLD224P
Connected seq. 
>SLD224P.Seq
CTTCTACATCAACTACTACAACAAAGATAAATGGCACCTCTAGTAGTAGTAGTAATAGTG
GTAGCAGTAGTATTAGTAAAAAAGCAGAAAAAGAATTAAAGGAACAAGAGAAACAAAAAA
TAAAAGAGTTGATGGATACCTTAAAATCAAAACAATTAACACCAGATCAAGAAAAAGCAA
TTAAATCACAAATTAAGCAACTCAAGAAAGAAAAAAGACAAAAGAAAGAAGAAAAGAAAT
TAGCAAAAAGATTAAAAGAAAAAGAAAAAGAAAGTAAATCAAAATCATCAACATCAACAT
CATCATCTAC----------TAATAATACCGAATGTAATAATAGTAAAATAAATGATGAA
TCATCAACTAACACCCCAACCACCTCAACCACCACAGCAACAAAGAAGAAAGGAGTAAAT
CATAATGTTGGACATATTATATCACTATGTATTGAAATTACCCATATCGTTAACAAGAGT
AAAGGTCAATTGCTTAGAGATATTGTTGATGAATGTTGCCTATTCCAAGGTCAATGGGAT
GAAGTTGTTGTACCAAAATTACGTCAAGAAATATCATTTAGAAATATCAAACCAATTGGT
ACCGTTTCAATGCGTAAAGGTAGAAAGGGTGCTCCACCAACTCCATTTGCAAATGTTGAA
TCTTTCCAAAATATGGCAAATGGTGTTCCAATTCCTCCAACAAATCCTTCCTCAAAATTC
TTTTTCTAAAAATATAATAATAATAATAATATAATAAAAAAACAAAACAAAAAACAAAAA
CAAAAATATAATAAACAAAAAAGA
Length of connected seq. 794
Full length Seq ID -
Full length Seq. -
Length of full length seq. -