>AFK248 (AFK248Q) /CSM/AF/AFK2-B/AFK248Q.Seq.d/
AATTTCAAAATTATTCAATCCAACATTTAAACAACATGATTTCATATTAGATTATCCAGA
TTTATATCATTCAAAAGAAAATATTGAAATATTTAGATATTTAGGTATCAGAGATAAATT
AAAGAAAAACGATATTGTAAATCAATTCAAAAAGATCTCATCAGAAGTTTATAGTGAAAA
TACACCTTTGAGCATTTTAATTGAAAGAGCACGTCTATTATGGAAATCAGAAAAATGTGT
CAAAGTATTTAATGAATTGTTAGAATTAAATATTGTACCAGTCAAAGAAAGTAATATCAT
TGGCAGTAGAAAAATATCATTAGGATTGATGCCAACTACCCGAAGTATGCCAACATCGCT
ATGAAGATCTTTATTTTTCAGTAAAACTACTTCAAGATGTAGATATTAAATACCCACCAG
GATATAATCAAATTGATTGTGAAAAAATATTTGAAAATTTCGTTAATCTTCTTACAAACA
TCAGAGATTGGAAATTGATTCAAGTAAATGGTAATTTAGTTACAGACCCCAAAATCATAC
AAATGTTAATATCGATGTGTAGTCATTTGGAAAACTATACAGGAAATTTCTCATCAGAAC
TTGTAGAAAXXXXXXXXXXTCAAGAATTTATTTCAAGAGTAAATCATCTTCAGTTAAGCG
GAATCTTGAATATTACAGTTAAAACAGGATATATTAGAACTAAATTCATTTTTAATCGAA
AAGATATCACTAAAACCTCAAAAACCAAATGCTTTTATGATCATTTAAATTCAACTCTTT
ATATAGGCATAGAAAAAGGTATTACCATAAGTAAAGCATTCAAATACCTATTTAACTCCA
CTGGTTTGAATGTAAACCCCTCAATGGTATCAAATCTAAAAAGAGAGTTTGTTAATGATG
AACCAACTGATGATAATTTATTCTTGACTCTAAACGTTGTTAATTTTGTTGATGTTAACC
AAACAAAAATCCAAAAAAATCAATGTAGACACTACAACACCATTGCATTAAGAAATTTTC
AACTTGCTGATACTTTACTCAACAATCAATCATTTATAAGAGAAGCCTCATACCATTACT
ATGTTTCAACAGAAATGTCAATGAGAAGTCTTTTGGTTTTTAATTATAATGATATCCCCT
ATGCTTTTTCAATTACTCGTTTAAGAGGTTTATTATCTAGAATAAGTATTACAACTGAAA
CCATACCACAGATTCCAGATCTTTACAATAATTACAGACACACTCATTTAAAAAATGAAG
AAATCAATCGTAGGGAACTATTAAGATTTCGTTCAATTTCAAATCAATTATTAACATCTC
AATCAAAAACTAA

fqnysiqhlnnmisy*iiqiyiiqkkilkyldi*vsein*rktil*inskrshqkfivki
hl*af*lkehvyygnqknvskylmnc*n*ilyqskkvislavekyh*d*CQLPEVCQHRY
EDLYFSVKLLQDVDIKYPPGYNQIDCEKIFENFVNLLTNIRDWKLIQVNGNLVTDPKIIQ
MLISMCSHLENYTGNFSSELVE---

---QEFISRVNHLQLSGILNITVKTGYIRTKFIFNRKDITKTSKTKCFYDHLNSTLYIGI
EKGITISKAFKYLFNSTGLNVNPSMVSNLKREFVNDEPTDDNLFLTLNVVNFVDVNQTKI
QKNQCRHYNTIALRNFQLADTLLNNQSFIREASYHYYVSTEMSMRSLLVFNYNDIPYAFS
ITRLRGLLSRISITTETIPQIPDLYNNYRHTHLKNEEINRRELLRFRSISNQLLTSQSKT

Frame A:
nfkiiqsni*tt*fhirlsrfisfkrky*ni*ifryqr*ikekrycksiqkdlirsl**k
ytfehfn*kstsimeirkmcqsi**ivrikyctsqrk*yhwq*kniiridanypkyania
mkififq*nyfkm*ilnthqdiiklivkkylkisliflqtseign*fk*mvi*lqtpksy
kc*yrcvviwktiqeishqnl*k---

---sriyfksksssvkrnleyys*nriy*n*ihf*skryh*nlknqmll*sfkfnslyrh
rkryyhk*siqipi*lhwfeckplngikskkrvc***tn***fildskrc*fc*c*pnkn
pkksm*tlqhhcikkfstc*yftqqsiiykrslipllcfnrnvneksfgf*l**yplcff
nysfkrfii*nkyyn*nhttdsrslq*lqthsfkk*rnqs*gtikisfnfksiinisikn
*

Frame B:
isklfnptfkqhdfildypdlyhskenieifrylgirdklkkndivnqfkkissevysen
tplsilierarllwksekcvkvfnellelnivpvkesniigsrkislglmpttrsmptsl
*rslffskttsrcry*iptri*sn*l*kni*kfr*ssykhqrleidsskw*fsyrpqnht
nvnidv*sfgklyrkflirtcr---

---QEFISRVNHLQLSGILNITVKTGYIRTKFIFNRKDITKTSKTKCFYDHLNSTLYIGI
EKGITISKAFKYLFNSTGLNVNPSMVSNLKREFVNDEPTDDNLFLTLNVVNFVDVNQTKI
QKNQCRHYNTIALRNFQLADTLLNNQSFIREASYHYYVSTEMSMRSLLVFNYNDIPYAFS
ITRLRGLLSRISITTETIPQIPDLYNNYRHTHLKNEEINRRELLRFRSISNQLLTSQSKT

Frame C:
fqnysiqhlnnmisy*iiqiyiiqkkilkyldi*vsein*rktil*inskrshqkfivki
hl*af*lkehvyygnqknvskylmnc*n*ilyqskkvislavekyh*d*CQLPEVCQHRY
EDLYFSVKLLQDVDIKYPPGYNQIDCEKIFENFVNLLTNIRDWKLIQVNGNLVTDPKIIQ
MLISMCSHLENYTGNFSSELVE---

---knlfqe*iifs*aes*ilqlkqdilelnsfliekislkpqkpnafmii*iqlfi*a*
kkvlp*vkhsntyltplv*m*tpqwyqi*kesllmmnqlmiiys*l*tllillmltkqks
kkinvdtttplh*eifnllilystinhl*ekphtitmfqqkcq*evfwfliimispmlfq
llv*evyyle*vlqlkpyhrfqiftiitdtli*kmkksivgny*dfvqfqiny*hlnqkl

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK248 (AFK248Q) /CSM/AF/AFK2-B/AFK248Q.Seq.d/                       2541   0.0
AHI139 (AHI139Q) /CSM/AH/AHI1-B/AHI139Q.Seq.d/                       1374   0.0
SHG595 (SHG595Q) /CSM/SH/SHG5-D/SHG595Q.Seq.d/                       1360   0.0
VHC628 (VHC628Q) /CSM/VH/VHC6-B/VHC628Q.Seq.d/                       1346   0.0
AHN318 (AHN318Q) /CSM/AH/AHN3-A/AHN318Q.Seq.d/                       1346   0.0
AHM294 (AHM294Q) /CSM/AH/AHM2-D/AHM294Q.Seq.d/                       1346   0.0
AHL406 (AHL406Q) /CSM/AH/AHL4-A/AHL406Q.Seq.d/                       1346   0.0
AHJ748 (AHJ748Q) /CSM/AH/AHJ7-B/AHJ748Q.Seq.d/                       1304   0.0
AHF686 (AHF686Q) /CSM/AH/AHF6-D/AHF686Q.Seq.d/                       1292   0.0
SSF181 (SSF181Q) /CSM/SS/SSF1-D/SSF181Q.Seq.d/                       1281   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF063866|AF063866.1 Melanoplus sanguinipes entomopoxvirus, complete genome.    42  3e-05  13
AP001119|AP001119.1 Buchnera sp. APS genomic DNA, complete sequence, segment 2/2.    32  0.012  14
AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    32  0.16  10
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    36  0.20  16
AP004172|AP004172.1 Mycoplasma penetrans genomic DNA, complete sequence, section 3/5.    32  0.24  12
AC112585|AC112585.4 Rattus norvegicus clone CH230-359E24, WORKING DRAFT SEQUENCE, 2 unordered pieces.    36  0.25  7
AC119929|AC119929.2 Mus musculus clone RP24-259B4, WORKING DRAFT SEQUENCE, 2 ordered pieces.    34  0.26  10
AV696014|AV696014.1 Homo sapiens cDNA clone:GKCBBB10, 5'end, expressed in human hepatocellular carcinoma.    36  0.50  2
AV695982|AV695982.1 Homo sapiens cDNA clone:GKCBBB08, 5'end, expressed in human hepatocellular carcinoma.    36  0.50  2
BX000526|BX000526.8 Zebrafish DNA sequence from clone BUSM1-87D10 in linkage group 14.    34  0.55  6

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF064079_1(AF064079|pid:none) Plasmodium gallinaceum endochitina...    40   0.18
AB106896_1(AB106896|pid:none) Plasmodium vivax pvcht1 gene for c...    40   0.23
AF533231_13(AF533231|pid:none) Western X phytoplasma tRNA-Ser ge...    39   0.40
AE017261_1169(AE017261|pid:none) Picrophilus torridus DSM 9790, ...    37   1.5
AM910992_217(AM910992|pid:none) Plasmodium knowlesi strain H chr...    36   2.6
CP000575_753(CP000575|pid:none) Staphylothermus marinus F1, comp...    36   2.6
AM910983_8(AM910983|pid:none) Plasmodium knowlesi strain H chrom...    35   4.4
AL138652_3(AL138652|pid:none) Arabidopsis thaliana DNA chromosom...    35   5.8
CP000025_301(CP000025|pid:none) Campylobacter jejuni RM1221, com...    35   5.8
DQ447636_1(DQ447636|pid:none) Artemisia annua germacrene A synth...    35   5.8

 psg: 0.66  gvh: 0.30  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: mitochondrial

>> prediction for AFK248 is cyt

>AFK248F.Seq
AATTTCAAAATTATTCAATCCAACATTTAAACAACATGATTTCATATTAGATTATCCAGA
TTTATATCATTCAAAAGAAAATATTGAAATATTTAGATATTTAGGTATCAGAGATAAATT
AAAGAAAAACGATATTGTAAATCAATTCAAAAAGATCTCATCAGAAGTTTATAGTGAAAA
TACACCTTTGAGCATTTTAATTGAAAGAGCACGTCTATTATGGAAATCAGAAAAATGTGT
CAAAGTATTTAATGAATTGTTAGAATTAAATATTGTACCAGTCAAAGAAAGTAATATCAT
TGGCAGTAGAAAAATATCATTAGGATTGATGCCAACTACCCGAAGTATGCCAACATCGCT
ATGAAGATCTTTATTTTTCAGTAAAACTACTTCAAGATGTAGATATTAAATACCCACCAG
GATATAATCAAATTGATTGTGAAAAAATATTTGAAAATTTCGTTAATCTTCTTACAAACA
TCAGAGATTGGAAATTGATTCAAGTAAATGGTAATTTAGTTACAGACCCCAAAATCATAC
AAATGTTAATATCGATGTGTAGTCATTTGGAAAACTATACAGGAAATTTCTCATCAGAAC
TTGTAGAAA----------

>AFK248Z.Seq
----------TCAAGAATTTATTTCAAGAGTAAATCATCTTCAGTTAAGCGGAATCTTGA
ATATTACAGTTAAAACAGGATATATTAGAACTAAATTCATTTTTAATCGAAAAGATATCA
CTAAAACCTCAAAAACCAAATGCTTTTATGATCATTTAAATTCAACTCTTTATATAGGCA
TAGAAAAAGGTATTACCATAAGTAAAGCATTCAAATACCTATTTAACTCCACTGGTTTGA
ATGTAAACCCCTCAATGGTATCAAATCTAAAAAGAGAGTTTGTTAATGATGAACCAACTG
ATGATAATTTATTCTTGACTCTAAACGTTGTTAATTTTGTTGATGTTAACCAAACAAAAA
TCCAAAAAAATCAATGTAGACACTACAACACCATTGCATTAAGAAATTTTCAACTTGCTG
ATACTTTACTCAACAATCAATCATTTATAAGAGAAGCCTCATACCATTACTATGTTTCAA
CAGAAATGTCAATGAGAAGTCTTTTGGTTTTTAATTATAATGATATCCCCTATGCTTTTT
CAATTACTCGTTTAAGAGGTTTATTATCTAGAATAAGTATTACAACTGAAACCATACCAC
AGATTCCAGATCTTTACAATAATTACAGACACACTCATTTAAAAAATGAAGAAATCAATC
GTAGGGAACTATTAAGATTTCGTTCAATTTCAAATCAATTATTAACATCTCAATCAAAAA
CTAA

>AFK248P.Seq
AATTTCAAAATTATTCAATCCAACATTTAAACAACATGATTTCATATTAGATTATCCAGA
TTTATATCATTCAAAAGAAAATATTGAAATATTTAGATATTTAGGTATCAGAGATAAATT
AAAGAAAAACGATATTGTAAATCAATTCAAAAAGATCTCATCAGAAGTTTATAGTGAAAA
TACACCTTTGAGCATTTTAATTGAAAGAGCACGTCTATTATGGAAATCAGAAAAATGTGT
CAAAGTATTTAATGAATTGTTAGAATTAAATATTGTACCAGTCAAAGAAAGTAATATCAT
TGGCAGTAGAAAAATATCATTAGGATTGATGCCAACTACCCGAAGTATGCCAACATCGCT
ATGAAGATCTTTATTTTTCAGTAAAACTACTTCAAGATGTAGATATTAAATACCCACCAG
GATATAATCAAATTGATTGTGAAAAAATATTTGAAAATTTCGTTAATCTTCTTACAAACA
TCAGAGATTGGAAATTGATTCAAGTAAATGGTAATTTAGTTACAGACCCCAAAATCATAC
AAATGTTAATATCGATGTGTAGTCATTTGGAAAACTATACAGGAAATTTCTCATCAGAAC
TTGTAGAAA----------TCAAGAATTTATTTCAAGAGTAAATCATCTTCAGTTAAGCG
GAATCTTGAATATTACAGTTAAAACAGGATATATTAGAACTAAATTCATTTTTAATCGAA
AAGATATCACTAAAACCTCAAAAACCAAATGCTTTTATGATCATTTAAATTCAACTCTTT
ATATAGGCATAGAAAAAGGTATTACCATAAGTAAAGCATTCAAATACCTATTTAACTCCA
CTGGTTTGAATGTAAACCCCTCAATGGTATCAAATCTAAAAAGAGAGTTTGTTAATGATG
AACCAACTGATGATAATTTATTCTTGACTCTAAACGTTGTTAATTTTGTTGATGTTAACC
AAACAAAAATCCAAAAAAATCAATGTAGACACTACAACACCATTGCATTAAGAAATTTTC
AACTTGCTGATACTTTACTCAACAATCAATCATTTATAAGAGAAGCCTCATACCATTACT
ATGTTTCAACAGAAATGTCAATGAGAAGTCTTTTGGTTTTTAATTATAATGATATCCCCT
ATGCTTTTTCAATTACTCGTTTAAGAGGTTTATTATCTAGAATAAGTATTACAACTGAAA
CCATACCACAGATTCCAGATCTTTACAATAATTACAGACACACTCATTTAAAAAATGAAG
AAATCAATCGTAGGGAACTATTAAGATTTCGTTCAATTTCAAATCAATTATTAACATCTC
AATCAAAAACTAA