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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLD185
Library SL
(Link to library)
Clone ID SLD185
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12145-1
Original site URL
Representative seq. ID SLD185P
(Link to Original site)
Representative DNA sequence 
>SLD185 (SLD185Q) /CSM/SL/SLD1-D/SLD185Q.Seq.d/
AACAACCAAATGATGTCACAAAAGAAAATAATGATAATACTTCTGAATCAAATGAAACAA
CAATAAATATACCACCAACAAACAATATCACCACCACCACTACTACCACTACAAATACGA
ATATCAATACXXXXXXXXXXAAACAATGTATCAACAACAACAGCAACAACAACAACAAAC
TGGTCAACCTGGAGGTCCAATGGGTGTCCAAAGAGGTGGCCATCTACCACCACAACACAA
CCACAACAACAACAACAACAACCACCACCACAATTCATTCAAGGTATTCCACCCGGTGCT
AATTTAGTCATCTCAAATGGTCCACCCAATCAACCATTTGTATTCCAAGGTGCTCACCCA
CCTTATGCCGTACCACACCTCAATACCCAATGCCACCCCAAGGTATTCAAGGTGGTAATA
AAAGATTTTATCAACCACCACCACAAGGTTATCCTCAAGTGCAACCAATGATAATTCCAC
AACAAGGTCAAGTTGTATCTCAAAATTCACCACAACAAGATTCTCCTTCAAATCGTTTAA
ATCAACAAGTTCCACCTTATTCTTATATGACTCACCCACCAAGAGGTTACCATCCAAATG
AAAATCAATATCATT
sequence update 1999. 2.23
Translated Amino Acid sequence 
QPNDVTKENNDNTSESNETTINIPPTNNITTTTTTTTNTNIN---

---NNVSTTTATTTTNWSTWRSNGCPKRWPSTTTTQPQQQQQQPPPQFIQGIPPGANLVI
SNGPPNQPFVFQGAHPPYAVPHLNTQCHPKVFKVVIKDFINHHHKVILKCNQ**fhnkvk
lylkihhnkillqiv*inkfhlili*lthqevtiqmkinii
Translated Amino Acid sequence (All Frames) 
Frame A:
nnqmmsqkkimiillnqmkqq*iyhqqtispppllplqirisi---

---kqcinnnsnnnnklvnlevqwvskevaiyhhnttttttttttttihsrystrc*fsh
lkwstqsticiprcsptlcrttpqypmppqgiqggnkrfyqpppqgypqvqpmiipqqgq
vvsqnspqqdspsnrlnqqvppysymthpprgyhpnenqyh

Frame B:
ttk*chkrk***yf*ik*nnnkyttnkqyhhhhyyhykyeyqy---

---NNVSTTTATTTTNWSTWRSNGCPKRWPSTTTTQPQQQQQQPPPQFIQGIPPGANLVI
SNGPPNQPFVFQGAHPPYAVPHLNTQCHPKVFKVVIKDFINHHHKVILKCNQ**fhnkvk
lylkihhnkillqiv*inkfhlili*lthqevtiqmkinii

Frame C:
QPNDVTKENNDNTSESNETTINIPPTNNITTTTTTTTNTNIN---

---tmyqqqqqqqqqtgqpggpmgvqrgghlppqhnhnnnnnnhhhnsfkvfhpvli*ss
qmvhpinhlyskvlthlmpyhtsipnatprysrw**kilsttttrlsssatndnsttrss
ciskftttrfsfksfkstsstlflydsptkrlpsk*ksis
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLD185 (SLD185Q) /CSM/SL/SLD1-D/SLD185Q.Seq.d/                        678   0.0
AFM594 (AFM594Q) /CSM/AF/AFM5-D/AFM594Q.Seq.d/                        634   0.0
VHQ159 (VHQ159Q) /CSM/VH/VHQ1-C/VHQ159Q.Seq.d/                        630   e-180
VHG405 (VHG405Q) /CSM/VH/VHG4-A/VHG405Q.Seq.d/                        597   e-170
VHG442 (VHG442Q) /CSM/VH/VHG4-B/VHG442Q.Seq.d/                        591   e-168
CHO774 (CHO774Q) /CSM/CH/CHO7-D/CHO774Q.Seq.d/                        549   e-155
VHO313 (VHO313Q) /CSM/VH/VHO3-A/VHO313Q.Seq.d/                        539   e-152
CHL729 (CHL729Q) /CSM/CH/CHL7-B/CHL729Q.Seq.d/                        373   e-102
VHE720 (VHE720Q) /CSM/VH/VHE7-A/VHE720Q.Seq.d/                        291   6e-78
SSH566 (SSH566Q) /CSM/SS/SSH5-C/SSH566Q.Seq.d/                        258   9e-68
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU052354) Dictyostelium discoideum slug cDNA, clone SLD185.      678   0.0    2
(BJ347367) Dictyostelium discoideum cDNA clone:dda26k23, 3' ...   440   0.0    3
(BJ446693) Dictyostelium discoideum cDNA clone:ddv63e15, 3' ...   438   0.0    3
(BJ440310) Dictyostelium discoideum cDNA clone:ddv43d12, 3' ...   398   e-180  3
(BJ440253) Dictyostelium discoideum cDNA clone:ddv43j02, 3' ...   369   e-178  5
(BJ385087) Dictyostelium discoideum cDNA clone:ddc51d19, 3' ...   357   e-168  3
(BJ445319) Dictyostelium discoideum cDNA clone:ddv59j03, 3' ...   347   e-165  3
(BJ382625) Dictyostelium discoideum cDNA clone:ddc45j07, 3' ...   357   2e-96  2
(BJ439325) Dictyostelium discoideum cDNA clone:ddv40h05, 3' ...   180   2e-71  2
(AU073567) Dictyostelium discoideum slug cDNA, clone SSH566.      258   2e-64  1
dna update 2009. 3.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55DE7) RecName: Full=Ataxin-2 homolog;                              108   3e-49
AE006641_2032(AE006641|pid:none) Sulfolobus solfataricus P2, com...    35   1.7
CP000544_896(CP000544|pid:none) Halorhodospira halophila SL1, co...    34   2.8
CU638744_144(CU638744|pid:none) Podospora anserina genomic DNA c...    33   4.9
protein update 2009. 6. 2
PSORT 

 psg: 0.75  gvh: 0.23  alm: 0.52  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall

>> prediction for SLD185 is nuc
5' end seq. ID SLD185F
5' end seq. 
>SLD185F.Seq
AACAACCAAATGATGTCACAAAAGAAAATAATGATAATACTTCTGAATCAAATGAAACAA
CAATAAATATACCACCAACAAACAATATCACCACCACCACTACTACCACTACAAATACGA
ATATCAATAC----------
Length of 5' end seq. 130
3' end seq. ID SLD185Z
3' end seq. 
>SLD185Z.Seq
----------AAACAATGTATCAACAACAACAGCAACAACAACAACAAACTGGTCAACCT
GGAGGTCCAATGGGTGTCCAAAGAGGTGGCCATCTACCACCACAACACAACCACAACAAC
AACAACAACAACCACCACCACAATTCATTCAAGGTATTCCACCCGGTGCTAATTTAGTCA
TCTCAAATGGTCCACCCAATCAACCATTTGTATTCCAAGGTGCTCACCCACCTTATGCCG
TACCACACCTCAATACCCAATGCCACCCCAAGGTATTCAAGGTGGTAATAAAAGATTTTA
TCAACCACCACCACAAGGTTATCCTCAAGTGCAACCAATGATAATTCCACAACAAGGTCA
AGTTGTATCTCAAAATTCACCACAACAAGATTCTCCTTCAAATCGTTTAAATCAACAAGT
TCCACCTTATTCTTATATGACTCACCCACCAAGAGGTTACCATCCAAATGAAAATCAATA
TCATT
Length of 3' end seq. 475
Connected seq. ID SLD185P
Connected seq. 
>SLD185P.Seq
AACAACCAAATGATGTCACAAAAGAAAATAATGATAATACTTCTGAATCAAATGAAACAA
CAATAAATATACCACCAACAAACAATATCACCACCACCACTACTACCACTACAAATACGA
ATATCAATAC----------AAACAATGTATCAACAACAACAGCAACAACAACAACAAAC
TGGTCAACCTGGAGGTCCAATGGGTGTCCAAAGAGGTGGCCATCTACCACCACAACACAA
CCACAACAACAACAACAACAACCACCACCACAATTCATTCAAGGTATTCCACCCGGTGCT
AATTTAGTCATCTCAAATGGTCCACCCAATCAACCATTTGTATTCCAAGGTGCTCACCCA
CCTTATGCCGTACCACACCTCAATACCCAATGCCACCCCAAGGTATTCAAGGTGGTAATA
AAAGATTTTATCAACCACCACCACAAGGTTATCCTCAAGTGCAACCAATGATAATTCCAC
AACAAGGTCAAGTTGTATCTCAAAATTCACCACAACAAGATTCTCCTTCAAATCGTTTAA
ATCAACAAGTTCCACCTTATTCTTATATGACTCACCCACCAAGAGGTTACCATCCAAATG
AAAATCAATATCATT
Length of connected seq. 605
Full length Seq ID -
Full length Seq. -
Length of full length seq. -