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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFK245
Library AF
(Link to library)
Clone ID AFK245
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFK245E
(Link to Original site)
Representative DNA sequence 
>AFK245 (AFK245Q) /CSM/AF/AFK2-B/AFK245Q.Seq.d/
ATCTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATA
CCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTAT
CAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTG
ATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTG
GTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAG
CTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGA
ATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATC
AATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTG
CCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAG
AAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAG
ATAACACCTACAAAGTACTCGTCGATAACAAAGAGATCCAAGCAGGTAACCTCGCTGATG
ATTGGGAATTACTTCCATCAAAACAAATCAAAGATCCAAAACAATCCAAACCAGTCGATT
GGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATA
TCCCAGCTTCAATCGTTGATCCAGAAGCTGCCAAACCAGAGGATTGGAATGAAGAAGACG
ACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAA
AGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTAATTGATAACCCAG
AATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAAT
TATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACCGACTCTGTCGAAG
AAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGT
TTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTG
CCGAAAAAGCTGCNAAAAAGAAGCCGAAGAAGCTATAAGAAGAAGAAGAAGTCGCNAAGA
AGATT
sequence update 2001. 6. 9
Translated Amino Acid sequence 
lsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSD
WHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKN
EQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKE
INKVETDQLTHQYTLVISPDNTYKVLVDNKEIQAGNLADDWELLPSKQIKDPKQSKPVDW
VDVKEIDDPEDVKPAGHDDIPASIVDPEAAKPEDWNEEDDGEWEAPTIANPEYKGEWKAK
KIPNPEYKGEWVHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEE
AKDFSEKTFVANQEAEKKMFDDLEAAKAEERKKADEKLAAEKAAKKKPKKL*eeeevakk
i
Translated Amino Acid sequence (All Frames) 
Frame A:
ispiiifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllv
igikkmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*r
mnkklivvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srk
kstklkpin*lintlwsfhqitptkyssitkrskqvtslmignyfhqnkskiqnnpnqsi
glt*rksmtqkmlnqpvmtisqlqsliqklpnqrigmkkttvngkpqpspiqntkvngkq
rksqiqntkvngyth*litqntlktmnstslmisvlsvsnygklnlvpslti*ssptlsk
klkislkrpllptkkpkrkclttskppklkrerkltkn*lpkklxkrsrrsykkkkksxr
r


Frame B:
slqssyl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**
la*rrw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke
*tks*lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqer
nqqs*nrsinssihfghftr*hlqstrr*qrdpsr*pr**lgitsiktnqrsktiqtsrl
g*rkgnr*prrc*tsrs*rypsfnr*srscqtrgle*rrrr*mgspnhrqsriqr*mesk
enpksriqr*mgtpin**prir*rq*tlpl**srcyrfrimas*iwfhl*qydrhrlcrr
s*rfl*kdlccqprsrkenv*rprsrqs*rekes*rkiscrkscxkeaeeairrrrsrxe
d


Frame C:
lsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSD
WHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKN
EQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKE
INKVETDQLTHQYTLVISPDNTYKVLVDNKEIQAGNLADDWELLPSKQIKDPKQSKPVDW
VDVKEIDDPEDVKPAGHDDIPASIVDPEAAKPEDWNEEDDGEWEAPTIANPEYKGEWKAK
KIPNPEYKGEWVHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEE
AKDFSEKTFVANQEAEKKMFDDLEAAKAEERKKADEKLAAEKAAKKKPKKL*eeeevakk
i
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK245 (AFK245Q) /CSM/AF/AFK2-B/AFK245Q.Seq.d/                       2125   0.0
AFN528 (AFN528Q) /CSM/AF/AFN5-B/AFN528Q.Seq.d/                       2117   0.0
AFK286 (AFK286Q) /CSM/AF/AFK2-D/AFK286Q.Seq.d/                       2117   0.0
AFE410 (AFE410Q) /CSM/AF/AFE4-A/AFE410Q.Seq.d/                       2117   0.0
AFE318 (AFE318Q) /CSM/AF/AFE3-A/AFE318Q.Seq.d/                       2117   0.0
AFE168 (AFE168Q) /CSM/AF/AFE1-C/AFE168Q.Seq.d/                       2117   0.0
AFD463 (AFD463Q) /CSM/AF/AFD4-C/AFD463Q.Seq.d/                       2117   0.0
AFJ570 (AFJ570Q) /CSM/AF/AFJ5-C/AFJ570Q.Seq.d/                       2111   0.0
AFA657 (AFA657Q) /CSM/AF/AFA6-C/AFA657Q.Seq.d/                       2111   0.0
AFG846 (AFG846Q) /CSM/AF/AFG8-B/AFG846Q.Seq.d/                       2109   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  2109   0.0    2
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92   8e-28  4
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   2e-21  4
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   1e-10  1
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74   2e-10  2
BT013428|BT013428.1 Lycopersicon esculentum clone 132068F, mRNA sequence.    74   4e-10  2
CK247437|CK247437.1 EST731074 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCAT37 5' end, mRNA sequence.    66   4e-09  3
CK253744|CK253744.1 EST737381 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCC781 5' end, mRNA sequence.    66   5e-09  3
AW626316|AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA sequence.    74   6e-09  1
BE459798|BE459798.1 EST415090 tomato developing/immature green fruit Lycopersicon esculentum cDNA clone cLEM8G13, mRNA sequence.    74   6e-09  1
dna update 2004. 7.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   684   0.0
AK318734_1(AK318734|pid:none) Arabidopsis thaliana AT1G56340 mRN...   421   e-116
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   417   e-115
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   416   e-115
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   416   e-114
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   416   e-114
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   416   e-114
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   416   e-114
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   414   e-114
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   413   e-114
protein update 2009. 6.29
PSORT 

 psg: 1.01  gvh: 0.69  alm: 0.47  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.29  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 28.0 %: nuclear
	 24.0 %: extracellular, including cell wall
	 24.0 %: mitochondrial
	  8.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: endoplasmic reticulum
	  4.0 %: vacuolar

>> prediction for AFK245 is nuc
5' end seq. ID AFK245F
5' end seq. 
>AFK245F.Seq
ATCTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATA
CCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTAT
CAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTG
ATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTG
GTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAG
CTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGA
ATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATC
AATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTG
CCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAG
AAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACAC----------
Length of 5' end seq. 583
3' end seq. ID AFK245Z
3' end seq. 
>AFK245Z.Seq
----------TTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCANCAAA
GTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTAC
AAAGTACTCGTCGATAACAAAGAGATCCAAGCAGGTAACCTCGCTGATGATTGGGAATTA
CTTCCATCAAAACAAATCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTA
AAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCA
ATCGTTGATCCAGAAGCTGCCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGG
GAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCA
AATCCAGAATACAAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAA
GACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTT
AAATCTGGTTCCATCTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGAT
TTCTCTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTC
GAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCT
GCNAAAAAGAAGCCGAAGAAGCTATAAGAAGAAGAAGAAGTCGCNAAGAAGATT
Length of 3' end seq. 764
Connected seq. ID AFK245P
Connected seq. 
>AFK245P.Seq
ATCTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATA
CCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTAT
CAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTG
ATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTG
GTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAG
CTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGA
ATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATC
AATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTG
CCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAG
AAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACAC----------TTCTCAA
CTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCANCAAAGTTGAAACCGATCAATT
AACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTACAAAGTACTCGTCGATAA
CAAAGAGATCCAAGCAGGTAACCTCGCTGATGATTGGGAATTACTTCCATCAAAACAAAT
CAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACCC
AGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGC
TGCCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGC
CAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGG
TGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCT
CTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTT
TAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTT
TGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGA
AGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCNAAAAAGAAGCCGAA
GAAGCTATAAGAAGAAGAAGAAGTCGCNAAGAAGATT
Length of connected seq. 1347
Full length Seq ID AFK245E
Full length Seq. 
>AFK245E.Seq
ATCTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATA
CCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTAT
CAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTG
ATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTG
GTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAG
CTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGA
ATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATC
AATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTG
CCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAG
AAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAG
ATAACACCTACAAAGTACTCGTCGATAACAAAGAGATCCAAGCAGGTAACCTCGCTGATG
ATTGGGAATTACTTCCATCAAAACAAATCAAAGATCCAAAACAATCCAAACCAGTCGATT
GGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATA
TCCCAGCTTCAATCGTTGATCCAGAAGCTGCCAAACCAGAGGATTGGAATGAAGAAGACG
ACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAA
AGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTAATTGATAACCCAG
AATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAAT
TATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACCGACTCTGTCGAAG
AAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGT
TTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTG
CCGAAAAAGCTGCNAAAAAGAAGCCGAAGAAGCTATAAGAAGAAGAAGAAGTCGCNAAGA
AGATT
Length of full length seq. 1265