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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFK242
Library AF
(Link to library)
Clone ID AFK242
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFK242P
(Link to Original site)
Representative DNA sequence 
>AFK242 (AFK242Q) /CSM/AF/AFK2-B/AFK242Q.Seq.d/
CACTGTTGGCCTACTGGAATTTCTCCAATGCATCATATTTATAATTATATCTTTTTTGTT
TAATAAAAAAGAAAGAATACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTT
GTTTTCAATTTTGCATTATCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAA
TCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCAT
ACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCT
CGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTT
CTCCAATACACTGTAAAGAATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTA
TTACCATCCAAATTAGATCAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTT
GGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAG
AACCATTTAATCAAGXXXXXXXXXXCAAATCAAAGATCCAAAACAATCCAAACCAGTCGA
TTGGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGA
TATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGA
CGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGC
AAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACNCCCATTAATTGATAACCC
AGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGA
ATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGA
AGAAGCTAAAGATTTCTNTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAAT
GTTTGACGACCTCGAAGCCGCCAAAGCTGAANANANAAAGAAAGCTGACGAAAAATTAGC
TGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGC
TGAAGAAGATTTAGTTAAAACTTAGNCAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAA
GT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
hcwptgispmhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWE
SRWVVSDWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLV
LQYTVKNEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGK
NHLIK---

---QIKDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWE
APTIANPEYKGEWKAKKIPNPEYKGEWVXPLIDNPEYAEDNELYLFNDLGAIGFELWQVK
SGSIFNNMIVXDSVEEAKDFXEKTFVANQEAEKKMFDDLEAAKAEXXKKADEKLAAEKAA
EKEAEEADEEEEEVAEEDLVKT*xkkkksknqpkk
Translated Amino Acid sequence (All Frames) 
Frame A:
hcwptgispmhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWE
SRWVVSDWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLV
LQYTVKNEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGK
NHLIK---

---QIKDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWE
APTIANPEYKGEWKAKKIPNPEYKGEWVXPLIDNPEYAEDNELYLFNDLGAIGFELWQVK
SGSIFNNMIVXDSVEEAKDFXEKTFVANQEAEKKMFDDLEAAKAEXXKKADEKLAAEKAA
EKEAEEADEEEEEVAEEDLVKT*xkkkksknqpkk

Frame B:
tvglleflqciifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitign
qdgllvigikkmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*f
sntl*rmnkklivvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttker
ti*s---

---kskiqnnpnqsiglt*rksmtqkmlnqpvmtisqlqsliqklsnqrigmkkttvngk
pqpspiqntkvngkqrksqiqntkvngyxh*litqntlktmnstslmisvlsvsnygkln
lvpslti*ssptlskklkisxkrpllptkkpkrkclttskppklxxxrkltkn*lpkkll
kkkpkklmkkkkkslkki*lklxqkrrsqkinqks

Frame C:
llaywnfsnasyl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgi
kmgc**la*rrw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfss
pihcke*tks*lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrke
pfnq---

---nqrsktiqtsrlg*rkgnr*prrc*tsrs*rypsfnr*srscqtrgle*rrrr*mgs
pnhrqsriqr*meskenpksriqr*mgtpin**prir*rq*tlpl**srcyrfrimas*i
wfhl*qydrxrlcrrs*rfl*kdlccqprsrkenv*rprsrqs*xxkes*rkiscrksc*
krsrrs**rrrrsr*rrfs*nlxkkeevkkstkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK242 (AFK242Q) /CSM/AF/AFK2-B/AFK242Q.Seq.d/                       2006   0.0
AFK274 (AFK274Q) /CSM/AF/AFK2-D/AFK274Q.Seq.d/                       1098   0.0
VFL283 (VFL283Q) /CSM/VF/VFL2-D/VFL283Q.Seq.d/                       1084   0.0
VFL214 (VFL214Q) /CSM/VF/VFL2-A/VFL214Q.Seq.d/                       1084   0.0
AFI321 (AFI321Q) /CSM/AF/AFI3-A/AFI321Q.Seq.d/                       1084   0.0
AFH646 (AFH646Q) /CSM/AF/AFH6-B/AFH646Q.Seq.d/                       1070   0.0
VFE283 (VFE283Q) /CSM/VF/VFE2-D/VFE283Q.Seq.d/                       1055   0.0
SFH596 (SFH596Q) /CSM/SF/SFH5-D/SFH596Q.Seq.d/                       1053   0.0
VFN642 (VFN642Q) /CSM/VF/VFN6-B/VFN642Q.Seq.d/                       1051   0.0
VFM435 (VFM435Q) /CSM/VF/VFM4-B/VFM435Q.Seq.d/                       1051   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1023  0.0  2
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92  7e-25  3
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    78  3e-21  4
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74  7e-11  2
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    78  3e-10  1
AI782264|AI782264.1 EST263143 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES18L5, mRNA sequence.    74  5e-09  1
AW033447|AW033447.1 EST277018 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC28B21 similar to calreticulin, putative, mRNA sequence.    74  5e-09  1
AI489195|AI489195.1 EST247534 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED17D1, mRNA sequence.    74  5e-09  1
AW649196|AW649196.1 EST327650 tomato germinating seedlings, TAMU Lycopersicon esculentum cDNA clone cLEI7M10 5', mRNA sequence.    74  5e-09  1
AW626316|AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA sequence.    74  5e-09  1
dna update 2003. 9. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   297   5e-79
L24159_1(L24159|pid:none) Schistosoma mansoni calreticulin gene,...   145   1e-67
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   192   2e-47
U66344_1(U66344|pid:none) Arabidopsis thaliana calreticulin (Crt...   189   1e-46
U27698_1(U27698|pid:none) Arabidopsis thaliana calreticulin (AtC...   189   1e-46
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   188   3e-46
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   187   5e-46
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   187   6e-46
AY086745_1(AY086745|pid:none) Arabidopsis thaliana clone 27210 m...   186   1e-45
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   186   1e-45
protein update 2009. 4.24
PSORT 

 psg: 1.01  gvh: 0.69  alm: 0.47  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: vacuolar
	  8.0 %: nuclear
	  8.0 %: endoplasmic reticulum

>> prediction for AFK242 is exc
5' end seq. ID AFK242F
5' end seq. 
>AFK242F.Seq
CACTGTTGGCCTACTGGAATTTCTCCAATGCATCATATTTATAATTATATCTTTTTTGTT
TAATAAAAAAGAAAGAATACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTT
GTTTTCAATTTTGCATTATCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAA
TCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCAT
ACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCT
CGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTT
CTCCAATACACTGTAAAGAATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTA
TTACCATCCAAATTAGATCAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTT
GGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAG
AACCATTTAATCAAG----------
Length of 5' end seq. 555
3' end seq. ID AFK242Z
3' end seq. 
>AFK242Z.Seq
----------CAAATCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAA
GGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAAT
CGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGA
AGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAA
TCCAGAATACAAAGGTGAATGGGTACNCCCATTAATTGATAACCCAGAATACGCTGAAGA
CAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAA
ATCTGGTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGAAGAAGCTAAAGATTT
CTNTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGA
AGCCGCCAAAGCTGAANANANAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGC
TGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGT
TAAAACTTAGNCAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGT
Length of 3' end seq. 637
Connected seq. ID AFK242P
Connected seq. 
>AFK242P.Seq
CACTGTTGGCCTACTGGAATTTCTCCAATGCATCATATTTATAATTATATCTTTTTTGTT
TAATAAAAAAGAAAGAATACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTT
GTTTTCAATTTTGCATTATCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAA
TCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCAT
ACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCT
CGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTT
CTCCAATACACTGTAAAGAATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTA
TTACCATCCAAATTAGATCAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTT
GGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAG
AACCATTTAATCAAG----------CAAATCAAAGATCCAAAACAATCCAAACCAGTCGA
TTGGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGA
TATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGA
CGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGC
AAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACNCCCATTAATTGATAACCC
AGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGA
ATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGA
AGAAGCTAAAGATTTCTNTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAAT
GTTTGACGACCTCGAAGCCGCCAAAGCTGAANANANAAAGAAAGCTGACGAAAAATTAGC
TGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGC
TGAAGAAGATTTAGTTAAAACTTAGNCAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAA
GT
Length of connected seq. 1192
Full length Seq ID -
Full length Seq. -
Length of full length seq. -