>AFK230 (AFK230Q) /CSM/AF/AFK2-B/AFK230Q.Seq.d/
TAGATGATAACCTCATCAATTCAATCAATAATAATAAAAAATCATCATGGACTGCCCATA
GAAATAAAAATTTCGAAGGTAAGACTTTTGGTGATATCATTGGTATGATGGGTACTAAAA
AAACTGXXXXXXXXXXGGCCAGACTGTATCCACCCAATCCTCAACCAAGAACAATGTGGT
TCATGTTGGGCCTTTTCTTCATCTGAAGTTTTAAGTGATAGATTATGTATTGCCTCAAAT
AATAAAACTAACCCAGGTGCTCTCAGTCCACAAACTTTAGTTGCTTGTGATGTATATGGT
AATGATGGTTGTAGTGGTGGTATCCCACAATTAGCTTGGGAATATATGGAACTTAAAGGT
TTACCAACTGACTCATGCGTCCCATACACTGCTGGTAACGGTACTGTCTACTCTTGTCAA
AGATCATGTTCCGATAGTGAAGATTACAGTTTATACAGAGCTAAGCCATTCACCTTAAAG
ACTTGCTCTTCAGTTCAATGTATCCAAGAAAACATTTTAGCTTATGGTCCAATCGTTGGT
ACTATGGAAGTTTATGAAGATTTTATGAGCTACAGCTCAGGTGTTTACGTTATGACTCCA
GGTTCATCTTTATTAGGTGGTCATGCTATTAAAATTGTTGGTTGGGGCTTTGATCAAACC
TCTCAATTAAACTACTGGATTGTTGCTAATTCATGGGGTGCTGACTGGGGTCAACAAGGT
TTCTTTTTCATTTCAATGGAAACTTGTTCAATTTCTAGTGATGCAAGTGCTGCCGAAGCC
CGTG

DDNLINSINNNKKSSWTAHRNKNFEGKTFGDIIGMMGTKKT---

---PDCIHPILNQEQCGSCWAFSSSEVLSDRLCIASNNKTNPGALSPQTLVACDVYGNDG
CSGGIPQLAWEYMELKGLPTDSCVPYTAGNGTVYSCQRSCSDSEDYSLYRAKPFTLKTCS
SVQCIQENILAYGPIVGTMEVYEDFMSYSSGVYVMTPGSSLLGGHAIKIVGWGFDQTSQL
NYWIVANSWGADWGQQGFFFISMETCSISSDASAAEAR

Frame A:
*mitssiqsiiiknhhglpieikiskvrllvislv*wvlkkl---

---gqtvstqsstknnvvhvgpflhlkf*vidyvlpqiikltqvlsvhkl*llvmymvmm
vvvvvshn*lgniwnlkvyqlthashtllvtvlstlvkdhvpivkitvytelshsp*rla
lqfnvskktf*lmvqslvlwkfmkil*ataqvftl*lqvhly*vvmllkllvgalikpln
*ttglllihgvltgvnkvsfsfqwklvqflvmqvlpkpv

Frame B:
r**phqfnq***kiimdcp*k*kfrr*dfw*yhwydgy*kn---

---arlyppnpqprtmwfmlglffi*sfk**imyclk**n*prcsqstnfscl*ciw**w
l*wwyptislgiygt*rftn*lmrpihcw*ryclllskimfr**rlqfiqs*aihlkdll
fssmyprkhfslwsnrwyygsl*rfyelqlrclrydsrfifirwscy*ncwlgl*snlsi
klldcc*fmgc*lgstrflfhfngnlfnf**ckccrsp

Frame C:
DDNLINSINNNKKSSWTAHRNKNFEGKTFGDIIGMMGTKKT---

---PDCIHPILNQEQCGSCWAFSSSEVLSDRLCIASNNKTNPGALSPQTLVACDVYGNDG
CSGGIPQLAWEYMELKGLPTDSCVPYTAGNGTVYSCQRSCSDSEDYSLYRAKPFTLKTCS
SVQCIQENILAYGPIVGTMEVYEDFMSYSSGVYVMTPGSSLLGGHAIKIVGWGFDQTSQL
NYWIVANSWGADWGQQGFFFISMETCSISSDASAAEAR

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFI135 (VFI135Q) /CSM/VF/VFI1-B/VFI135Q.Seq.d/                       1285   0.0
VFG553 (VFG553Q) /CSM/VF/VFG5-C/VFG553Q.Seq.d/                       1285   0.0
AFK230 (AFK230Q) /CSM/AF/AFK2-B/AFK230Q.Seq.d/                       1285   0.0
VFO122 (VFO122Q) /CSM/VF/VFO1-A/VFO122Q.Seq.d/                       1283   0.0
VFI602 (VFI602Q) /CSM/VF/VFI6-A/VFI602Q.Seq.d/                       1283   0.0
VFD411 (VFD411Q) /CSM/VF/VFD4-A/VFD411Q.Seq.d/                       1277   0.0
VFB763 (VFB763Q) /CSM/VF/VFB7-C/VFB763Q.Seq.d/                       1277   0.0
VFF125 (VFF125Q) /CSM/VF/VFF1-B/VFF125Q.Seq.d/                       1271   0.0
SFC182 (SFC182Q) /CSM/SF/SFC1-D/SFC182Q.Seq.d/                       1269   0.0
VSJ659 (VSJ659Q) /CSM/VS/VSJ6-C/VSJ659Q.Seq.d/                       1257   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC140034|AC140034.4 Medicago truncatula clone mth2-17g19, WORKING DRAFT SEQUENCE, 2 ordered pieces.    48  0.17  1
AC142240|AC142240.2 Rattus norvegicus clone CH230-481N20, WORKING DRAFT SEQUENCE, 16 unordered pieces.    48  0.17  1
BZ262244|BZ262244.1 CH230-409I5.TJ CHORI-230 Segment 2 Rattus norvegicus genomic clone CH230-409I5, DNA sequence.    48  0.17  1
CF088632|CF088632.1 QHM1G24.yg.ab1 QH_M sunflower H.argophyllus Helianthus argophyllus cDNA clone QHM1G24, mRNA sequence.    48  0.17  1
AX251414|AX251414.1 Sequence 382 from Patent WO0168912.           40  0.20  2
AC078913|AC078913.6 Mus musculus chromosome 19 clone RP23-345G6 strain C57BL6/J, WORKING DRAFT SEQUENCE, 47 unordered pieces.    36  0.51  3
AL355097|AL355097.5 Human chromosome 14 DNA sequence BAC R-204N11 of library RPCI-11  from chromosome 14 of Homo sapiens (Human).    44  0.54  2
AC117226|AC117226.3 Mus musculus clone RP23-377C22, complete sequence.    34  0.62  5
AC117586|AC117586.5 Mus musculus clone RP23-166M8, *** SEQUENCING IN PROGRESS ***, 6 unordered pieces.    40  1.7  7
AL445594|AL445594.3 Human chromosome 14 DNA sequence BAC R-74H1 of library RPCI-11 from chromosome 14 of Homo sapiens (Human).    44  1.8  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY204512_1(AY204512|pid:none) Sterkiella histriomuscorum catheps...   178   2e-43
DQ363675_1(DQ363675|pid:none) Streblomastix strix cathepsin B ge...   176   7e-43
U51892_1(U51892|pid:none) Ascaris suum cathepsin B-like cysteine...   155   2e-36
EF474111_1(EF474111|pid:none) Monocercomonoides sp. PA cathepsin...   151   2e-35
EF474114_1(EF474114|pid:none) Monocercomonoides sp. PA cathepsin...   151   2e-35
DQ993253_1(DQ993253|pid:none) Hippoglossus hippoglossus cathepsi...   150   3e-35
EF474109_1(EF474109|pid:none) Monocercomonoides sp. PA cathepsin...   150   4e-35
U41556_2(U41556|pid:none) Caenorhabditis elegans cosmid C25B8, c...   149   7e-35
(P43510) RecName: Full=Cathepsin B-like cysteine proteinase 6;  ...   149   7e-35
AB371620_1(AB371620|pid:none) Tuberaphis coreana catB mRNA for c...   149   7e-35

 psg: 0.75  gvh: 0.41  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFK230 is cyt

>AFK230F.Seq
TAGATGATAACCTCATCAATTCAATCAATAATAATAAAAAATCATCATGGACTGCCCATA
GAAATAAAAATTTCGAAGGTAAGACTTTTGGTGATATCATTGGTATGATGGGTACTAAAA
AAACTG----------

>AFK230Z.Seq
----------GGCCAGACTGTATCCACCCAATCCTCAACCAAGAACAATGTGGTTCATGT
TGGGCCTTTTCTTCATCTGAAGTTTTAAGTGATAGATTATGTATTGCCTCAAATAATAAA
ACTAACCCAGGTGCTCTCAGTCCACAAACTTTAGTTGCTTGTGATGTATATGGTAATGAT
GGTTGTAGTGGTGGTATCCCACAATTAGCTTGGGAATATATGGAACTTAAAGGTTTACCA
ACTGACTCATGCGTCCCATACACTGCTGGTAACGGTACTGTCTACTCTTGTCAAAGATCA
TGTTCCGATAGTGAAGATTACAGTTTATACAGAGCTAAGCCATTCACCTTAAAGACTTGC
TCTTCAGTTCAATGTATCCAAGAAAACATTTTAGCTTATGGTCCAATCGTTGGTACTATG
GAAGTTTATGAAGATTTTATGAGCTACAGCTCAGGTGTTTACGTTATGACTCCAGGTTCA
TCTTTATTAGGTGGTCATGCTATTAAAATTGTTGGTTGGGGCTTTGATCAAACCTCTCAA
TTAAACTACTGGATTGTTGCTAATTCATGGGGTGCTGACTGGGGTCAACAAGGTTTCTTT
TTCATTTCAATGGAAACTTGTTCAATTTCTAGTGATGCAAGTGCTGCCGAAGCCCGTG

>AFK230P.Seq
TAGATGATAACCTCATCAATTCAATCAATAATAATAAAAAATCATCATGGACTGCCCATA
GAAATAAAAATTTCGAAGGTAAGACTTTTGGTGATATCATTGGTATGATGGGTACTAAAA
AAACTG----------GGCCAGACTGTATCCACCCAATCCTCAACCAAGAACAATGTGGT
TCATGTTGGGCCTTTTCTTCATCTGAAGTTTTAAGTGATAGATTATGTATTGCCTCAAAT
AATAAAACTAACCCAGGTGCTCTCAGTCCACAAACTTTAGTTGCTTGTGATGTATATGGT
AATGATGGTTGTAGTGGTGGTATCCCACAATTAGCTTGGGAATATATGGAACTTAAAGGT
TTACCAACTGACTCATGCGTCCCATACACTGCTGGTAACGGTACTGTCTACTCTTGTCAA
AGATCATGTTCCGATAGTGAAGATTACAGTTTATACAGAGCTAAGCCATTCACCTTAAAG
ACTTGCTCTTCAGTTCAATGTATCCAAGAAAACATTTTAGCTTATGGTCCAATCGTTGGT
ACTATGGAAGTTTATGAAGATTTTATGAGCTACAGCTCAGGTGTTTACGTTATGACTCCA
GGTTCATCTTTATTAGGTGGTCATGCTATTAAAATTGTTGGTTGGGGCTTTGATCAAACC
TCTCAATTAAACTACTGGATTGTTGCTAATTCATGGGGTGCTGACTGGGGTCAACAAGGT
TTCTTTTTCATTTCAATGGAAACTTGTTCAATTTCTAGTGATGCAAGTGCTGCCGAAGCC
CGTG