>AFK221 (AFK221Q) /CSM/AF/AFK2-A/AFK221Q.Seq.d/
CATAATAAAACCGTCAACAACCAATAATAATAATAAGAATAAAATATTATAATAATAATA
ATAATAAAAATATAATAATATAAATTTTATTTTTTATTTTTTTTTTAATTTATAAATTTT
ATAAAAAATAAAATGAAATCTATATTAAAGAAAGCAAAACATTTCTTTCACACCAATGAA
ACAGTAAATGGAGAAAATGGTGGTGAAAAAACAGCAAAAGAGAGTGAAAGTCAACAACAC
CACCAACAACAACAACAACATGATGAAAATGGTGCAAACCCACCAGACCAAGGAGTTGAT
GAAGCATCAAATGTATCACAATCTCAACCAACTACATCAGCATTACAAACTTCAACCTCA
TTACAACCATCATCATCATTACATCAAATTCCTCAATCACAATCTAGCTTAGAATTAACA
ACTAATCCAACACAACAACTACCAACAACACCAACCAAACAATTACCAACACCACCACCA
CCACAACAACCACATTCACAACAACAACAACAACAACAACAACAATCACAGTCACAATTA
AATAATAATGATATTAGXXXXXXXXXX

hnktvnnq****e*niiiiiiiki**ykfyflfff*FINFIKNKMKSILKKAKHFFHTNE
TVNGENGGEKTAKESESQQHHQQQQQHDENGANPPDQGVDEASNVSQSQPTTSALQTSTS
LQPSSSLHQIPQSQSSLELTTNPTQQLPTTPTKQLPTPPPPQQPHSQQQQQQQQQSQSQL
NNNDI---

Frame A:
hnktvnnq****e*niiiiiiiki**ykfyflfff*FINFIKNKMKSILKKAKHFFHTNE
TVNGENGGEKTAKESESQQHHQQQQQHDENGANPPDQGVDEASNVSQSQPTTSALQTSTS
LQPSSSLHQIPQSQSSLELTTNPTQQLPTTPTKQLPTPPPPQQPHSQQQQQQQQQSQSQL
NNNDI---


Frame B:
iikpsttnnnnknkil******kynninfifyfffnl*il*kik*nly*rkqnisftpmk
q*mekmvvkkqqkrvkvnnttnnnnnmmkmvqthqtkelmkhqmyhnlnqlhqhyklqph
ynhhhhyikflnhnla*n*qliqhnnyqqhqpnnyqhhhhhnnhihnnnnnnnnnhshn*
iimil---


Frame C:
**nrqqpiiiirikyynnnnnkniii*ilffiffliykfykk*neiyikesktflshq*n
skwrkww*knskre*ksttppttttt**kwckptrprs**sikcitistnyisitnfnli
ttiiiitsnssiti*lrinn*snttttnntnqtitnttttttttftttttttttitvtik
***y---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK221 (AFK221Q) /CSM/AF/AFK2-A/AFK221Q.Seq.d/                        327   1e-88
CHP109 (CHP109Q) /CSM/CH/CHP1-A/CHP109Q.Seq.d/                        313   2e-84
SLG413 (SLG413Q) /CSM/SL/SLG4-A/SLG413Q.Seq.d/                         64   2e-09
VHM195 (VHM195Q) /CSM/VH/VHM1-D/VHM195Q.Seq.d/                         42   0.009
VHG275 (VHG275Q) /CSM/VH/VHG2-D/VHG275Q.Seq.d/                         42   0.009
VHG175 (VHG175Q) /CSM/VH/VHG1-D/VHG175Q.Seq.d/                         42   0.009
CHG579 (CHG579Q) /CSM/CH/CHG5-D/CHG579Q.Seq.d/                         42   0.009
VHM478 (VHM478Q) /CSM/VH/VHM4-D/VHM478Q.Seq.d/                         38   0.14
CHC890 (CHC890Q) /CSM/CH/CHC8-D/CHC890Q.Seq.d/                         38   0.14
VHN213 (VHN213Q) /CSM/VH/VHN2-A/VHN213Q.Seq.d/                         36   0.56

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF238312|AF238312.1 Dictyostelium discoideum putative serine-threonine protein kinase MkcB (mkcB) gene, complete cds.   327  e-137  4
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    36  0.63  4
BG570152|BG570152.1 602591142F1 NIH_MGC_77 Homo sapiens cDNA clone IMAGE:4717763 5', mRNA sequence.    34  1.6  2
AC128614|AC128614.2 Rattus norvegicus clone CH230-272A3, WORKING DRAFT SEQUENCE, 1 ordered piece.    44  1.9  1
AC007019|AC007019.5 Arabidopsis thaliana chromosome 2 clone F7D8 map mi238, complete sequence.    44  1.9  1
AC131540|AC131540.5 Rattus norvegicus clone CH230-27C20, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    44  1.9  1
AL137068|AL137068.10 Human DNA sequence from clone RP11-165P4 on chromosome  9q34.11-34.13.    36  3.4  2
AC007232|AC007232.6 Arabidopsis thaliana chromosome 2 clone T16B14 map mi238, complete sequence.    44  4.5  2
AC006558|AC006558.1 Homo sapiens chromosome 9 clone hRPK.477_J_21 map 9, WORKING DRAFT SEQUENCE, 7 unordered pieces.    36  4.6  2
AF164347|AF164347.1 Dictyostelium discoideum developmental protein DG1113 (DG1113) gene, complete cds.    38  6.4  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AE014297_1244(AE014297|pid:none) Drosophila melanogaster chromos...    42   0.011
A26480(A26480) knob protein - malaria parasite (Plasmodium falci...    37   0.26
AF275687_1(AF275687|pid:none) Plasmodium falciparum knob-associa...    37   0.26
A54495(A54495) knob protein precursor - malaria parasite (Plasmo...    37   0.26
(P13817) RecName: Full=Knob-associated histidine-rich protein;  ...    37   0.26
AE014298_375(AE014298|pid:none) Drosophila melanogaster chromoso...    37   0.34
(Q9NBW1) RecName: Full=Frizzled-4; AltName: Full=dFz4; Flags: Pr...    37   0.45
CT005251_49(CT005251|pid:none) Leishmania major strain Friedlin,...    36   0.59
EU100924_1(EU100924|pid:none) Apis dorsata isolate DorsIII6Thai ...    36   0.76
AE013599_884(AE013599|pid:none) Drosophila melanogaster chromoso...    36   0.76

 psg: 0.53  gvh: 0.26  alm: 0.63  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 24.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFK221 is nuc

>AFK221F.Seq
CATAATAAAACCGTCAACAACCAATAATAATAATAAGAATAAAATATTATAATAATAATA
ATAATAAAAATATAATAATATAAATTTTATTTTTTATTTTTTTTTTAATTTATAAATTTT
ATAAAAAATAAAATGAAATCTATATTAAAGAAAGCAAAACATTTCTTTCACACCAATGAA
ACAGTAAATGGAGAAAATGGTGGTGAAAAAACAGCAAAAGAGAGTGAAAGTCAACAACAC
CACCAACAACAACAACAACATGATGAAAATGGTGCAAACCCACCAGACCAAGGAGTTGAT
GAAGCATCAAATGTATCACAATCTCAACCAACTACATCAGCATTACAAACTTCAACCTCA
TTACAACCATCATCATCATTACATCAAATTCCTCAATCACAATCTAGCTTAGAATTAACA
ACTAATCCAACACAACAACTACCAACAACACCAACCAAACAATTACCAACACCACCACCA
CCACAACAACCACATTCACAACAACAACAACAACAACAACAACAATCACAGTCACAATTA
AATAATAATGATATTAG----------