>SLC473 (SLC473Q) /CSM/SL/SLC4-D/SLC473Q.Seq.d/
AGAAATTGGATAACTGTGAATTCTATGCACTTCCAAAGAGTCAAGAACAAGCTTGGAATG
AAATCACCGTCGATGGTCTTTGGACCACCGCTACCAATGGTGGTGGTGATATCAATATCT
TGGGTTGGACAAAGAATCCACAATACTCTTTCACCTTGACAAAGAAATCTAGAGCTTGTG
TATTACTCAGTCAAGATGATAATGATAAGAGTGTTGGTTTTTATGTTATCAAACAATTGG
ATGCTGGTAAACGTGCTATCGAATTCAGAGAACAAGTCGGTAAAACTGAATCCTTCAAAT
TCTCTTGTTCCACTGGTTGCACTTTAACCCTCGACGAAGGTACATACATTGTCATTCCAT
CAACTTATGATCATGGTATCGAAGGTGCTTTCCATTTAACACTTTTCACCGATGATAAGA
ATGCCACCTTCCAACCATTAACAAACGCTTTCCAAGAGGTTGAACAAGTTAAAGGTACTT
GGGTTGGTAAATCTGCTGGTGGTTCTCCAAATCAACCAACTTTCTTCAATAATCCACAAT
TCCATTTGAAAGTACCTGCCTCTGATAAAGATGAAGTCATCGCCGTTCAATTGATTCAAG
ATTCAACCATTGCCGATGAAGGTATTGGTTTCATTGTTTTATCTCGTGATTCTCATTCTG
AACCATTGACTGCTCAAGATTTCCAAAATGAAATGGTTXXXXXXXXXX

KLDNCEFYALPKSQEQAWNEITVDGLWTTATNGGGDINILGWTKNPQYSFTLTKKSRACV
LLSQDDNDKSVGFYVIKQLDAGKRAIEFREQVGKTESFKFSCSTGCTLTLDEGTYIVIPS
TYDHGIEGAFHLTLFTDDKNATFQPLTNAFQEVEQVKGTWVGKSAGGSPNQPTFFNNPQF
HLKVPASDKDEVIAVQLIQDSTIADEGIGFIVLSRDSHSEPLTAQDFQNEM---

Frame A:
rnwitvnsmhfqrvknklgmkspsmvfgpplpmvvvisiswvgqrihntlsp*qrnlelv
yysvkmimirvlvfmlsnnwmlvnvlsnsenksvklnpsnslvplval*pstkvhtlsfh
qlmimvskvlsi*hfspmirmppsnh*qtlskrlnklkvlglvnllvvlqinqlssiihn
si*kylplikmksspfn*fkiqplpmkvlvslfylvililnh*llkiskmkw---


Frame B:
eig*l*ilctskesrtsle*nhrrwsldhryqwww*yqylgldkestilfhldkei*slc
itqsr****ecwflcyqtigcw*tcyriqrtsr*n*ilqillfhwlhfnprrryihchsi
nl*swyrrcfpfntfhr**echlptinkrfprg*ts*rylgw*icwwfskstnflq*sti
pfestcl**r*shrrsidsrfnhcr*rywfhcfis*fsf*tidcsrfpk*ng---


Frame C:
KLDNCEFYALPKSQEQAWNEITVDGLWTTATNGGGDINILGWTKNPQYSFTLTKKSRACV
LLSQDDNDKSVGFYVIKQLDAGKRAIEFREQVGKTESFKFSCSTGCTLTLDEGTYIVIPS
TYDHGIEGAFHLTLFTDDKNATFQPLTNAFQEVEQVKGTWVGKSAGGSPNQPTFFNNPQF
HLKVPASDKDEVIAVQLIQDSTIADEGIGFIVLSRDSHSEPLTAQDFQNEM---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLC473 (SLC473Q) /CSM/SL/SLC4-D/SLC473Q.Seq.d/                       1384   0.0
SFH493 (SFH493Q) /CSM/SF/SFH4-D/SFH493Q.Seq.d/                       1096   0.0
VHL339 (VHL339Q) /CSM/VH/VHL3-B/VHL339Q.Seq.d/                       1043   0.0
VFL249 (VFL249Q) /CSM/VF/VFL2-C/VFL249Q.Seq.d/                        916   0.0
SLC787 (SLC787Q) /CSM/SL/SLC7-D/SLC787Q.Seq.d/                        910   0.0
SSC516 (SSC516Q) /CSM/SS/SSC5-A/SSC516Q.Seq.d/                        823   0.0
SLF205 (SLF205Q) /CSM/SL/SLF2-A/SLF205Q.Seq.d/                        743   0.0
VFO135 (VFO135Q) /CSM/VF/VFO1-B/VFO135Q.Seq.d/                         40   0.045
VFM323 (VFM323Q) /CSM/VF/VFM3-A/VFM323Q.Seq.d/                         40   0.045
VFK711 (VFK711Q) /CSM/VF/VFK7-A/VFK711Q.Seq.d/                         40   0.045

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AF525418) Dictyostelium discoideum calpain-like cysteine pr...  1384   0.0    1
(AU060977) Dictyostelium discoideum slug cDNA, clone SLC473.     1384   0.0    1
(BJ401030) Dictyostelium discoideum cDNA clone:dds15j24, 3' ...  1096   0.0    1
(BJ443590) Dictyostelium discoideum cDNA clone:ddv53n09, 3' ...   807   0.0    2
(BJ433961) Dictyostelium discoideum cDNA clone:ddv23a14, 3' ...   916   0.0    1
(AU034383) Dictyostelium discoideum slug cDNA, clone SLC787.      910   0.0    1
(AU052847) Dictyostelium discoideum slug cDNA, clone SLF205.      743   0.0    1
(AU071783) Dictyostelium discoideum slug cDNA, clone SSC516.      476   e-130  1
(BJ415713) Dictyostelium discoideum cDNA clone:ddv23a14, 5' ...   379   e-100  1
(AU061062) Dictyostelium discoideum slug cDNA, clone SLC787.      180   5e-41  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC125867_1(BC125867|pid:none) Danio rerio zgc:153446, mRNA (cDNA...    53   1e-05
CT005260_15(CT005260|pid:none) Leishmania major strain Friedlin,...    52   2e-05
(Q7RZP7) RecName: Full=Calpain-like protease palB/rim-13;       ...    52   2e-05
AY639153_1(AY639153|pid:none) Gecarcinus lateralis calpain B (Ca...    52   2e-05
BX649315_3(BX649315|pid:none) Zebrafish DNA sequence from clone ...    48   2e-04
(Q95LP4) RecName: Full=Calpain-10;          EC=3.4.22.-; AltName...    48   2e-04
BX004801_1(BX004801|pid:none) Zebrafish DNA sequence from clone ...    48   2e-04
(Q9VT65) RecName: Full=Calpain-B;          EC=3.4.22.-; AltName:...    48   3e-04
AM502239_177(AM502239|pid:none) Leishmania infantum chromosome 21.     48   3e-04
AF062404_1(AF062404|pid:none) Drosophila melanogaster calpain mR...    48   3e-04

 psg: 0.75  gvh: 0.49  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for SLC473 is cyt

>SLC473F.Seq
AGAAATTGGATAACTGTGAATTCTATGCACTTCCAAAGAGTCAAGAACAAGCTTGGAATG
AAATCACCGTCGATGGTCTTTGGACCACCGCTACCAATGGTGGTGGTGATATCAATATCT
TGGGTTGGACAAAGAATCCACAATACTCTTTCACCTTGACAAAGAAATCTAGAGCTTGTG
TATTACTCAGTCAAGATGATAATGATAAGAGTGTTGGTTTTTATGTTATCAAACAATTGG
ATGCTGGTAAACGTGCTATCGAATTCAGAGAACAAGTCGGTAAAACTGAATCCTTCAAAT
TCTCTTGTTCCACTGGTTGCACTTTAACCCTCGACGAAGGTACATACATTGTCATTCCAT
CAACTTATGATCATGGTATCGAAGGTGCTTTCCATTTAACACTTTTCACCGATGATAAGA
ATGCCACCTTCCAACCATTAACAAACGCTTTCCAAGAGGTTGAACAAGTTAAAGGTACTT
GGGTTGGTAAATCTGCTGGTGGTTCTCCAAATCAACCAACTTTCTTCAATAATCCACAAT
TCCATTTGAAAGTACCTGCCTCTGATAAAGATGAAGTCATCGCCGTTCAATTGATTCAAG
ATTCAACCATTGCCGATGAAGGTATTGGTTTCATTGTTTTATCTCGTGATTCTCATTCTG
AACCATTGACTGCTCAAGATTTCCAAAATGAAATGGTT----------