>AFK208 (AFK208Q) /CSM/AF/AFK2-A/AFK208Q.Seq.d/
GGCCTACTGGTATTCTATTCCACACACCCAAAGTATTCATATTAATGGCAGAAATAACAA
TATCAATTGAAAATGAAAATTTCATTCAAGTAAATTTACAACCAGATCAAACAGTTGAAG
ATCTAAAACGTAGAGTAGAATTTGAAACCACAATTTTAGTGAACAATCAAGTTTTAACAC
TTGATGGAAAAGTATTAGATAATGAAAAGAAATTATCAGACTATTCAATTAAAGGTGGTG
ATTTCTTATTGATAACAAAAAATGTTTTAAGAGCACCACAACAAAGATCACAACAACCCC
AACAACCACAACAACCACAACAACCACAACAACAACGTCAACCACAAAGAGATCCTCTCA
ATTCACCACAAGATATTTTAGACCACTTTACAAACAATCCTGAAGACTTAACTCAAGTTA
TAAATTCAAATCCAGCATTAGCAAATGCAATCTTAAGTAAAGATATGAAATTCCTTACTC
ACTTTGNAGAACAAATCANAGAACANCGTCNTATTCAAGAATTGGCATTAAAAGXXXXXX
XXXX

PTGILFHTPKVFILMAEITISIENENFIQVNLQPDQTVEDLKRRVEFETTILVNNQVLTL
DGKVLDNEKKLSDYSIKGGDFLLITKNVLRAPQQRSQQPQQPQQPQQPQQQRQPQRDPLN
SPQDILDHFTNNPEDLTQVINSNPALANAILSKDMKFLTHFXEQIXEXRXIQELALK---

Frame A:
gllvfysthpkysy*wqk*qyqlkmkisfk*iynqikqlki*nve*nlkpqf**tikf*h
lmeky*imkrnyqtiqlkvvisy**qkmf*ehhnkdhnnpnnhnnhnnhnnnvnhkeils
ihhkif*ttlqtilkt*lkl*iqiqh*qmqs*vki*nslltlxnksxnxvxfknwh*---


Frame B:
aywysiphtqsihingrnnnin*k*kfhsskfttrsns*rskt*sri*nhnfseqssfnt
*wksir**keiirlfn*rw*flidnkkcfkstttkitttpttttttttttttsttkrssq
fttryfrplykqs*rlnssykfkssiskcnlk*ryeipyslxrtnxrtxsysrigik---


Frame C:
PTGILFHTPKVFILMAEITISIENENFIQVNLQPDQTVEDLKRRVEFETTILVNNQVLTL
DGKVLDNEKKLSDYSIKGGDFLLITKNVLRAPQQRSQQPQQPQQPQQPQQQRQPQRDPLN
SPQDILDHFTNNPEDLTQVINSNPALANAILSKDMKFLTHFXEQIXEXRXIQELALK---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK208 (AFK208Q) /CSM/AF/AFK2-A/AFK208Q.Seq.d/                        539   e-152
VFE249 (VFE249Q) /CSM/VF/VFE2-C/VFE249Q.Seq.d/                        504   e-142
VFE196 (VFE196Q) /CSM/VF/VFE1-D/VFE196Q.Seq.d/                        504   e-142
SLC513 (SLC513Q) /CSM/SL/SLC5-A/SLC513Q.Seq.d/                        504   e-142
SFL472 (SFL472Q) /CSM/SF/SFL4-C/SFL472Q.Seq.d/                        504   e-142
SFL211 (SFL211Q) /CSM/SF/SFL2-A/SFL211Q.Seq.d/                        504   e-142
SFK703 (SFK703Q) /CSM/SF/SFK7-A/SFK703Q.Seq.d/                        504   e-142
SFK605 (SFK605Q) /CSM/SF/SFK6-A/SFK605Q.Seq.d/                        504   e-142
SFJ818 (SFJ818Q) /CSM/SF/SFJ8-A/SFJ818Q.Seq.d/                        504   e-142
SFJ734 (SFJ734Q) /CSM/SF/SFJ7-B/SFJ734Q.Seq.d/                        504   e-142

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC104442|AC104442.2 Homo sapiens chromosome 3 clone RP11-905F6, complete sequence.    40  0.024  3
AE001387|AE001387.1 Plasmodium falciparum chromosome 2, section 24 of 73 of the complete sequence.    38  0.45  3
AL885102|AL885102.1 Xenopus tropicalis EST, clone TEgg018e06 3'.    46  0.46  1
AL672272|AL672272.10 Mouse DNA sequence from clone RP23-47N2 on chromosome 4.    40  0.73  3
AK122228|AK122228.1 Mus musculus mRNA for mKIAA0241 protein.      44  0.75  2
AC131444|AC131444.4 Rattus norvegicus clone CH230-5A7, *** SEQUENCING IN PROGRESS ***, 8 unordered pieces.    40  1.3  5
AC114430|AC114430.5 Rattus norvegicus clone CH230-11L18, WORKING DRAFT SEQUENCE, 9 unordered pieces.    46  1.3  3
AP004170|AP004170.1 Mycoplasma penetrans genomic DNA, complete sequence, section 1/5.    40  1.6  6
AC132632|AC132632.4 Rattus norvegicus clone CH230-228B17, WORKING DRAFT SEQUENCE, 1 ordered piece.    44  1.8  1
AC116290|AC116290.4 Rattus norvegicus clone CH230-100A13, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    44  1.8  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54JB0) RecName: Full=Protein DDI1 homolog;                          251   6e-66
AK067974_1(AK067974|pid:none) Oryza sativa Japonica Group cDNA c...    59   8e-08
BT058466_1(BT058466|pid:none) Salmo salar clone Contig1073 UV ex...    46   7e-04
CR940348_825(CR940348|pid:none) Theileria annulata strain Ankara...    44   0.003
BC055645_1(BC055645|pid:none) Danio rerio neural precursor cell ...    43   0.004
BC078600_1(BC078600|pid:none) Xenopus laevis hypothetical protei...    43   0.004
BC076245_1(BC076245|pid:none) Danio rerio neural precursor cell ...    43   0.004
EF103394_1(EF103394|pid:none) Haliotis discus discus ubiquitin-l...    42   0.007
AB024034_5(AB024034|pid:none) Arabidopsis thaliana genomic DNA, ...    42   0.007
AY085083_1(AY085083|pid:none) Arabidopsis thaliana clone 12734 m...    42   0.007

 psg: 0.88  gvh: 0.35  alm: 0.37  top: 0.47  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 68.0 %: cytoplasmic
	 24.0 %: nuclear
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for AFK208 is cyt

>AFK208F.Seq
GGCCTACTGGTATTCTATTCCACACACCCAAAGTATTCATATTAATGGCAGAAATAACAA
TATCAATTGAAAATGAAAATTTCATTCAAGTAAATTTACAACCAGATCAAACAGTTGAAG
ATCTAAAACGTAGAGTAGAATTTGAAACCACAATTTTAGTGAACAATCAAGTTTTAACAC
TTGATGGAAAAGTATTAGATAATGAAAAGAAATTATCAGACTATTCAATTAAAGGTGGTG
ATTTCTTATTGATAACAAAAAATGTTTTAAGAGCACCACAACAAAGATCACAACAACCCC
AACAACCACAACAACCACAACAACCACAACAACAACGTCAACCACAAAGAGATCCTCTCA
ATTCACCACAAGATATTTTAGACCACTTTACAAACAATCCTGAAGACTTAACTCAAGTTA
TAAATTCAAATCCAGCATTAGCAAATGCAATCTTAAGTAAAGATATGAAATTCCTTACTC
ACTTTGNAGAACAAATCANAGAACANCGTCNTATTCAAGAATTGGCATTAAAAG------
----