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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLC428
Library SL
(Link to library)
Clone ID SLC428
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10963-1
Original site URL
Representative seq. ID SLC428P
(Link to Original site)
Representative DNA sequence 
>SLC428 (SLC428Q) /CSM/SL/SLC4-B/SLC428Q.Seq.d/
GATGAACTCATTCGTTTATTGGATCCAGGTGTACCATTATGGAAACCAACCAAAGGTAAA
TCAAATATAGTAATGTTTGTAGGTTTACAAGGTGCTGGTAAAACCACATCTGTCACTAAA
TTAGCCTATTTTTATAAGAAGAAAGGTTTTTCAACTGCAATCGTTTGTGCTGATACTTTT
CGTGCTGGTGCATTCGACCAAGTACGTCACAATGCCGCAAAGGCAAAGATCCACTACTAT
GGTAGCGAGACTGAGAAGGATCCAGTCGTAGTAGCACGTACTGGTGTTGATATCTTTAAA
AAGGATGGAACAGAGATCATCATCGTCGATACATCAGGTAGACATAAACAAGATAGTGAG
TTATTTGAAGAGATGAAACAAATTGAAACAGCAGTCAAACCTGACAATTGTATTTTCGTT
ATGGATAGTTCAATTGGTCAAGCTGCATACGAACAAGCAACAGCATTTAGAAGTTCAGTT
AAAGTTGGTTCAATCATTATCACCAAAATGGATGGTAATTCAATGGGTGGTGGTGCAATC
TCTGCTGTTGCCGCCACTAATACACCTATCATTXXXXXXXXXXGTAGAGGAGGTGGTGGT
ATGCCAAATATGGGTGACCTCGCCAAAATGATGGGAGGCATGGGAGGTGGTGCTGGTAAA
GGTGGTCAAAATGGTTTCCCTAATCTTGACTTGGATTAAATTTAAACTTTAAAATACAAT
TTTGCGACCTATTTATCATCATATTTTATAACTCTATATCCTAACAACACTTTTATAATG
TATAATTCCAAACAATTTCCAAAAAAAAAAAAAAAAAAAAAAATAAATAAAAAAAAATAA
ATAAAAAAAAATAAATAAAAAAAATTTAAATAATAAAAAAAAAAAAAAAA
sequence update 1999. 2.19
Translated Amino Acid sequence 
DELIRLLDPGVPLWKPTKGKSNIVMFVGLQGAGKTTSVTKLAYFYKKKGFSTAIVCADTF
RAGAFDQVRHNAAKAKIHYYGSETEKDPVVVARTGVDIFKKDGTEIIIVDTSGRHKQDSE
LFEEMKQIETAVKPDNCIFVMDSSIGQAAYEQATAFRSSVKVGSIIITKMDGNSMGGGAI
SAVAATNTPII---

---RGGGGMPNMGDLAKMMGGMGGGAGKGGQNGFPNLDLD*i*tlkynfatylssyfitl
ypnntfimynskqfpkkkkkkkinkkk*ikknk*kkfk**kkkk
Translated Amino Acid sequence (All Frames) 
Frame A:
DELIRLLDPGVPLWKPTKGKSNIVMFVGLQGAGKTTSVTKLAYFYKKKGFSTAIVCADTF
RAGAFDQVRHNAAKAKIHYYGSETEKDPVVVARTGVDIFKKDGTEIIIVDTSGRHKQDSE
LFEEMKQIETAVKPDNCIFVMDSSIGQAAYEQATAFRSSVKVGSIIITKMDGNSMGGGAI
SAVAATNTPII---

---veevvvcqiwvtspk*weawevvlvkvvkmvsliltwikfkl*ntilrpiyhhil*l
yilttll*ciipnnfqkkkkkkk*ikknk*kkinkknlnnkkkkk

Frame B:
mnsfvywiqvyhygnqpkvnqi**cl*vykvlvkphlsln*pifirrkvfqlqsfvlilf
vlvhstkyvtmpqrqrsttmvarlrriqs**hvlvlislkrmeqrssssihqvdinkivs
ylkr*nklkqqsnltivfslwivqlvklhtnkqqhlevqlklvqslspkwmviqwvvvqs
lllpplihls---

---*rrwwyakyg*prqndgrhgrwcw*rwskwfp*s*lglnlnfkiqfcdlfiiifyns
is*qhfynv*fqtiskkkkkkknk*kkinkkk*ikki*iikkkkk

Frame C:
*thsfigsrctimetnqr*ikysnvcrftrcw*nhich*islfl*eerffncnrlc*yfs
cwcirpstsqcrkgkdpllw*rd*egssrsstywc*yl*kgwnrdhhrryir*t*tr**v
i*rdetn*nssqt*qlyfryg*fnwsscirtsnsi*kfs*swfnhyhqngw*fngwwcnl
cccrh*ytyh---

---RGGGGMPNMGDLAKMMGGMGGGAGKGGQNGFPNLDLD*i*tlkynfatylssyfitl
ypnntfimynskqfpkkkkkkkinkkk*ikknk*kkfk**kkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLC428 (SLC428Q) /CSM/SL/SLC4-B/SLC428Q.Seq.d/                       1526   0.0
CFF540 (CFF540Q) /CSM/CF/CFF5-B/CFF540Q.Seq.d/                        694   0.0
CFD462 (CFD462Q) /CSM/CF/CFD4-C/CFD462Q.Seq.d/                        694   0.0
CFJ846 (CFJ846Q) /CSM/CF/CFJ8-B/CFJ846Q.Seq.d/                        668   0.0
VFI442 (VFI442Q) /CSM/VF/VFI4-B/VFI442Q.Seq.d/                        618   e-176
AFL842 (AFL842Q) /CSM/AF/AFL8-B/AFL842Q.Seq.d/                        521   e-147
CFH249 (CFH249Q) /CSM/CF/CFH2-C/CFH249Q.Seq.d/                        500   e-140
VFH893 (VFH893Q) /CSM/VF/VFH8-D/VFH893Q.Seq.d/                        498   e-140
AHJ272 (AHJ272Q) /CSM/AH/AHJ2-C/AHJ272Q.Seq.d/                        498   e-140
AHH339 (AHH339Q) /CSM/AH/AHH3-B/AHH339Q.Seq.d/                        498   e-140
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC114258) Dictyostelium discoideum chromosome 2 map complem...  1136   0.0    1
(AU060970) Dictyostelium discoideum slug cDNA, clone SLC428.     1136   0.0    1
(BJ431998) Dictyostelium discoideum cDNA clone:ddv17d12, 3' ...   557   0.0    2
(BJ360743) Dictyostelium discoideum cDNA clone:ddc7l16, 5' e...   694   0.0    1
(BJ359017) Dictyostelium discoideum cDNA clone:ddc12o09, 5' ...   694   0.0    1
(BJ362131) Dictyostelium discoideum cDNA clone:ddc20l12, 5' ...   668   0.0    1
(BJ413885) Dictyostelium discoideum cDNA clone:ddv17d12, 5' ...   618   e-173  1
(BJ329241) Dictyostelium discoideum cDNA clone:dda24d12, 5' ...   470   e-140  2
(BJ359587) Dictyostelium discoideum cDNA clone:ddc15a14, 5' ...   500   e-137  1
(BJ413763) Dictyostelium discoideum cDNA clone:ddv16j24, 5' ...   498   e-136  1
dna update 2009. 3. 2
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q75K18) RecName: Full=Signal recognition particle 54 kDa protei...   378   e-103
(Q8MZJ6) RecName: Full=Signal recognition particle 54 kDa protei...   266   5e-70
BT044934_1(BT044934|pid:none) Salmo salar clone ssal-rgf-507-335...   262   1e-68
BT043608_1(BT043608|pid:none) Salmo salar clone HM4_3367 signal ...   262   1e-68
(Q6AYB5) RecName: Full=Signal recognition particle 54 kDa protei...   261   2e-68
BC005543_1(BC005543|pid:none) Mus musculus signal recognition pa...   260   4e-68
(P61010) RecName: Full=Signal recognition particle 54 kDa protei...   260   5e-68
AK293319_1(AK293319|pid:none) Homo sapiens cDNA FLJ54343 complet...   260   5e-68
DQ646413_1(DQ646413|pid:none) Bombyx mori putative signal recogn...   259   1e-67
BC061368_1(BC061368|pid:none) Xenopus tropicalis signal recognit...   259   1e-67
protein update 2009. 6. 1
PSORT 

 psg: 0.75  gvh: 0.44  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SLC428 is cyt
5' end seq. ID SLC428F
5' end seq. 
>SLC428F.Seq
GATGAACTCATTCGTTTATTGGATCCAGGTGTACCATTATGGAAACCAACCAAAGGTAAA
TCAAATATAGTAATGTTTGTAGGTTTACAAGGTGCTGGTAAAACCACATCTGTCACTAAA
TTAGCCTATTTTTATAAGAAGAAAGGTTTTTCAACTGCAATCGTTTGTGCTGATACTTTT
CGTGCTGGTGCATTCGACCAAGTACGTCACAATGCCGCAAAGGCAAAGATCCACTACTAT
GGTAGCGAGACTGAGAAGGATCCAGTCGTAGTAGCACGTACTGGTGTTGATATCTTTAAA
AAGGATGGAACAGAGATCATCATCGTCGATACATCAGGTAGACATAAACAAGATAGTGAG
TTATTTGAAGAGATGAAACAAATTGAAACAGCAGTCAAACCTGACAATTGTATTTTCGTT
ATGGATAGTTCAATTGGTCAAGCTGCATACGAACAAGCAACAGCATTTAGAAGTTCAGTT
AAAGTTGGTTCAATCATTATCACCAAAATGGATGGTAATTCAATGGGTGGTGGTGCAATC
TCTGCTGTTGCCGCCACTAATACACCTATCATT----------
Length of 5' end seq. 573
3' end seq. ID SLC428Z
3' end seq. 
>SLC428Z.Seq
----------GTAGAGGAGGTGGTGGTATGCCAAATATGGGTGACCTCGCCAAAATGATG
GGAGGCATGGGAGGTGGTGCTGGTAAAGGTGGTCAAAATGGTTTCCCTAATCTTGACTTG
GATTAAATTTAAACTTTAAAATACAATTTTGCGACCTATTTATCATCATATTTTATAACT
CTATATCCTAACAACACTTTTATAATGTATAATTCCAAACAATTTCCAAAAAAAAAAAAA
AAAAAAAAAATAAATAAAAAAAAATAAATAAAAAAAAATAAATAAAAAAAATTTAAATAA
TAAAAAAAAAAAAAAAA
Length of 3' end seq. 307
Connected seq. ID SLC428P
Connected seq. 
>SLC428P.Seq
GATGAACTCATTCGTTTATTGGATCCAGGTGTACCATTATGGAAACCAACCAAAGGTAAA
TCAAATATAGTAATGTTTGTAGGTTTACAAGGTGCTGGTAAAACCACATCTGTCACTAAA
TTAGCCTATTTTTATAAGAAGAAAGGTTTTTCAACTGCAATCGTTTGTGCTGATACTTTT
CGTGCTGGTGCATTCGACCAAGTACGTCACAATGCCGCAAAGGCAAAGATCCACTACTAT
GGTAGCGAGACTGAGAAGGATCCAGTCGTAGTAGCACGTACTGGTGTTGATATCTTTAAA
AAGGATGGAACAGAGATCATCATCGTCGATACATCAGGTAGACATAAACAAGATAGTGAG
TTATTTGAAGAGATGAAACAAATTGAAACAGCAGTCAAACCTGACAATTGTATTTTCGTT
ATGGATAGTTCAATTGGTCAAGCTGCATACGAACAAGCAACAGCATTTAGAAGTTCAGTT
AAAGTTGGTTCAATCATTATCACCAAAATGGATGGTAATTCAATGGGTGGTGGTGCAATC
TCTGCTGTTGCCGCCACTAATACACCTATCATT----------GTAGAGGAGGTGGTGGT
ATGCCAAATATGGGTGACCTCGCCAAAATGATGGGAGGCATGGGAGGTGGTGCTGGTAAA
GGTGGTCAAAATGGTTTCCCTAATCTTGACTTGGATTAAATTTAAACTTTAAAATACAAT
TTTGCGACCTATTTATCATCATATTTTATAACTCTATATCCTAACAACACTTTTATAATG
TATAATTCCAAACAATTTCCAAAAAAAAAAAAAAAAAAAAAAATAAATAAAAAAAAATAA
ATAAAAAAAAATAAATAAAAAAAATTTAAATAATAAAAAAAAAAAAAAAA
Length of connected seq. 880
Full length Seq ID -
Full length Seq. -
Length of full length seq. -