>AFK185 (AFK185Q) /CSM/AF/AFK1-D/AFK185Q.Seq.d/
ATATATATCATTTTTTAAAATGAGCGTCATCAAATCAATTAAAGCCAGAGAAATTTTAGA
TTCACGTGGTAACCCAACTGTTGAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTC
ATCATTCAGAGCTGCAGTTCCATCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATT
AAGAGATGGTGATAAATCAAGATACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACAT
TCTCGAAGTCATTCAACCAGCCGTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGA
TAAATTAATGATCGATCTCGATGGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTAT
CCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTA
CAAATATATCTCAGAAATTGCAGGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGT
TATCAATGGTGGTTCACATGCAGGTAATAAATTAGCTATGCAAGAATTTATGATTTTACC
AGTTGGTGCTAAAGATTTCAATGAAGCCTATCGTATGGGTTCAGAAGTTTATCATAACTT
AAAGAATGTCATCAGTGGTAGATACXXXXXXXXXX

YISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSGASTGIYEAVEL
RDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTPNKGKLGANAI
LAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGNKLAMQEFMILP
VGAKDFNEAYRMGSEVYHNLKNVISGR---

Frame A:
iyiif*nerhqin*sqrnfrftw*pnc*s*plhrkrwccfiiqscssircinwyl*sc*i
krw**ikilr*rctqsnqkhsrshstsrhw*isfrsscyr*indrsrwypk*r*ircqry
pcsqfsclqswcc**kfttlqiylrncry*neitsssiqcyqwwftcr**isyariydft
swc*rfq*slsygfrsls*lkechqw*i---


Frame B:
YISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSGASTGIYEAVEL
RDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTPNKGKLGANAI
LAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGNKLAMQEFMILP
VGAKDFNEAYRMGSEVYHNLKNVISGR---


Frame C:
iyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfhqvhqlvfmklln*
emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sismvpqikvn*vptls
lqsv*lfaelvllieiyhftnisqklqvlk*dyqfqhsmlsmvvhmqvin*lcknl*fyq
lvlkismkpivwvqkfiit*rmssvvd---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB851 (VFB851Q) /CSM/VF/VFB8-C/VFB851Q.Seq.d/                       1239   0.0
SSM734 (SSM734Q) /CSM/SS/SSM7-B/SSM734Q.Seq.d/                       1239   0.0
SLA770 (SLA770Q) /CSM/SL/SLA7-C/SLA770Q.Seq.d/                       1239   0.0
SFH339 (SFH339Q) /CSM/SF/SFH3-B/SFH339Q.Seq.d/                       1239   0.0
SFC211 (SFC211Q) /CSM/SF/SFC2-A/SFC211Q.Seq.d/                       1239   0.0
AFO869 (AFO869Q) /CSM/AF/AFO8-C/AFO869Q.Seq.d/                       1239   0.0
AFO860 (AFO860Q) /CSM/AF/AFO8-C/AFO860Q.Seq.d/                       1239   0.0
AFK185 (AFK185Q) /CSM/AF/AFK1-D/AFK185Q.Seq.d/                       1239   0.0
AFE445 (AFE445Q) /CSM/AF/AFE4-B/AFE445Q.Seq.d/                       1239   0.0
AFD204 (AFD204Q) /CSM/AF/AFD2-A/AFD204Q.Seq.d/                       1239   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BI504877|BI504877.1 BB170011A20G10.5 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170011A20G10 5', mRNA sequence.   125  2e-31  2
AX619982|AX619982.1 Sequence 2945 from Patent WO02094868.         50  2e-28  6
BD237140|BD237140.1 Staphylococcus aureus gene and polypeptide.    50  4e-28  6
AR282358|AR282358.1 Sequence 21 from patent US 6521441.           50  4e-28  6
AF065394|AF065394.1 Staphylococcus aureus enolase (eno) gene, complete cds.    50  4e-28  6
AR354089|AR354089.1 Sequence 207 from patent US 6593114.          50  5e-28  6
BE580531|BE580531.1 kq41g08.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:T21B10.2 CE03684 ENOLASE ;, mRNA sequence.    78  6e-27  3
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.          86  7e-27  5
BI511389|BI511389.1 BB160005B10G04 Bee Brain Normalized Library, BB16 Apis mellifera cDNA clone BB160005B10G04 5', mRNA sequence.   125  7e-25  1
BM164058|BM164058.1 EST566581 PyBS Plasmodium yoelii yoelii cDNA clone PYCLP21 5' end, mRNA sequence.    82  6e-24  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   391   e-108
BC045082_1(BC045082|pid:none) Xenopus laevis enolase 3, beta mus...   275   6e-73
EF137905_1(EF137905|pid:none) Helianthus annuus plastid enolase ...   275   7e-73
BC096516_1(BC096516|pid:none) Xenopus tropicalis hypothetical pr...   275   1e-72
DQ497988_1(DQ497988|pid:none) Polypterus senegalus enolase B mRN...   273   2e-72
DQ497989_1(DQ497989|pid:none) Typhlonectes natans enolase B mRNA...   273   3e-72
DQ497985_1(DQ497985|pid:none) Polypterus senegalus enolase A mRN...   271   8e-72
(Q9W7L0) RecName: Full=Alpha-enolase;          EC=4.2.1.11; AltN...   271   8e-72
BC054169_1(BC054169|pid:none) Xenopus laevis enolase 1, alpha, m...   271   1e-71
AC016662_10(AC016662|pid:none) Arabidopsis thaliana chromosome 1...   271   1e-71

 psg: 0.57  gvh: 0.46  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFK185 is cyt

>AFK185F.Seq
ATATATATCATTTTTTAAAATGAGCGTCATCAAATCAATTAAAGCCAGAGAAATTTTAGA
TTCACGTGGTAACCCAACTGTTGAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTC
ATCATTCAGAGCTGCAGTTCCATCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATT
AAGAGATGGTGATAAATCAAGATACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACAT
TCTCGAAGTCATTCAACCAGCCGTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGA
TAAATTAATGATCGATCTCGATGGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTAT
CCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTA
CAAATATATCTCAGAAATTGCAGGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGT
TATCAATGGTGGTTCACATGCAGGTAATAAATTAGCTATGCAAGAATTTATGATTTTACC
AGTTGGTGCTAAAGATTTCAATGAAGCCTATCGTATGGGTTCAGAAGTTTATCATAACTT
AAAGAATGTCATCAGTGGTAGATAC----------