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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFK178
Library AF
(Link to library)
Clone ID AFK178
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U09907-1
Original site URL
Representative seq. ID AFK178P
(Link to Original site)
Representative DNA sequence 
>AFK178 (AFK178Q) /CSM/AF/AFK1-D/AFK178Q.Seq.d/
AAAAATGAATAAAGAAANANGAAAATGAACAAAATACATTTTGTCTCATTCATTCAACAC
AGGAAATAACATTTATTTGCACTTCATGTAAATTTTCACCTGCTTGCAATAAGTGTGTGG
TTTTAAAGGGTATTCACTTTGGTCATGATATTATAGAAATCGATGATGAAAGTGTTTTAC
CAATTTTAAAAGATTGAATGAAAAAACAATACCAAAAATTGATGAATTAATAAAAAACAA
TCAAGATTTACTTAAAGGTAATTTAACAAAATATGAAGAAATTAAAGAAAAACATAAATC
AAATTTACAAATTATCAGTGGCCAAATTATGAAATTACATACAATTTTGCAAACTGTTGA
AANTAATTNAAATCAACAATTAATTACAAATTTATATGAAAATACTGATATTAATGATGT
TTCCXXXXXXXXXXTGATTATAATTTCATTGATGAAAGCCAAAATTTAAATGATAAACAC
ATTCAAATCATTAAAGATTCATATCAAAGTAAAAAGATTTTGACATGTCATTTTAGTGAT
TTGCCAGATTATTCAAGTTCAAAGGTTACCATTTCAGAGTCATCATTTAATTCAATTAAA
GATTTGTTTAATTTAATAATTAGTATTGATAAATACCATGGACAATTGCCTCCTACTAAT
GAACATTCACCTCTAAAACAGTTGTCAAGATATAGATGGGGTAAATTTATATTTACAGTT
TACAATGATGGTGATAGTATACCAGATGATACAGAATCTCTTGCAATTGGAGAGGGACAG
CGTCTACCTAAAACCATGCCAAAATCAACCAAAAGATTATTATTGCCCAATGGATTTAAT
CAATCATTAATATCATTACCAAAAACTGTTAGATCACTTTACCTTTTTAACATTGGTCTT
CAATTGACTGAGGCTTCAATTCCTGAAACTGTAGATACACTCTTCTTTTGTGATGGATTT
AGTCAAAAAAATTACAAAGGGAATAATTCCAAAATCAGTTAAAGAGGTGTTTTTCTATAA
TATAGTTCAACCACTTGAAAAAGATTCTATCCCAAACACTGTTTCATTTGTGGGTCGTTC
CCCTTCCTTTAAACATCCTCTTGATGTTAAATCAATAAATCAAATTAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
kmnkexxk*tkyilshsfntgnniylhfm*iftclq*vcgfkgyslws*yyrnr**KCFT
NFKRLNEKTIPKIDELIKNNQDLLKGNLTKYEEIKEKHKSNLQIISGQIMKLHTILQTVE
XNXNQQLITNLYENTDINDVS---

---DYNFIDESQNLNDKHIQIIKDSYQSKKILTCHFSDLPDYSSSKVTISESSFNSIKDL
FNLIISIDKYHGQLPPTNEHSPLKQLSRYRWGKFIFTVYNDGDSIPDDTESLAIGEGQRL
PKTMPKSTKRLLLPNGFNQSLISLPKTVRSLYLFNIGLQLTEASIPETVDTLFFCDGFSQ
KNYKGNNSKIS*rgvfl*ysstt*krfypkhcficgsfpfl*tss*c*inksn*
Translated Amino Acid sequence (All Frames) 
Frame A:
kne*rxxkmnkihfvsfiqhrk*hlfalhvnfhllaisvwf*rvftlvmil*ksmmkvfy
qf*kie*knntkn**inkkqsrft*r*fnki*rn*rkt*ikftnyqwpnyeitynfanc*
x*xkstinykfi*ky*y**cf---

---*l*fh**kpkfk**thsnh*rfisk*kdfdmsf**farlfkfkgyhfrvii*fn*rf
v*fnn*y**ipwtiasy**tftsktvvki*mg*iyiyslq*w**ytr*yriscnwrgtas
t*nhakinqkiiiaqwi*siiniitknc*itlpf*hwssid*gfns*ncrytlll*wi*s
kklqre*fqnqlkrcfsii*fnhlkkilsqtlfhlwvvplplnillmlnq*ikl

Frame B:
kmnkexxk*tkyilshsfntgnniylhfm*iftclq*vcgfkgyslws*yyrnr**KCFT
NFKRLNEKTIPKIDELIKNNQDLLKGNLTKYEEIKEKHKSNLQIISGQIMKLHTILQTVE
XNXNQQLITNLYENTDINDVS---

---DYNFIDESQNLNDKHIQIIKDSYQSKKILTCHFSDLPDYSSSKVTISESSFNSIKDL
FNLIISIDKYHGQLPPTNEHSPLKQLSRYRWGKFIFTVYNDGDSIPDDTESLAIGEGQRL
PKTMPKSTKRLLLPNGFNQSLISLPKTVRSLYLFNIGLQLTEASIPETVDTLFFCDGFSQ
KNYKGNNSKIS*rgvfl*ysstt*krfypkhcficgsfpfl*tss*c*inksn*

Frame C:
k*ikkxeneqntfclihstqeitfictsckfspacnkcvvlkgihfghdiieiddesvlp
ilkd*mkkqyqklmn**ktikiylkvi*qnmkklkkninqiyklsvakl*nyiqfckllk
xixinn*lqiymkililmmf---

---iiislmkaki*mintfkslkihikvkrf*hvilvicqiiqvqrlpfqshhliqlkic
li**lvlintmdnclllmnihl*nscqdidgvnlylqftmmvivyqmiqnllqlerdsvy
lkpcqnqpkdyycpmdlinh*yhyqklldhftfltlvfn*lrlqflkl*ihssfvmdlvk
kitkgiipksvkevffynivqplekdsipntvsfvgrspsfkhpldvksinqi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK178 (AFK178Q) /CSM/AF/AFK1-D/AFK178Q.Seq.d/                       2111   0.0
AFH120 (AFH120Q) /CSM/AF/AFH1-A/AFH120Q.Seq.d/                       1326   0.0
AFO886 (AFO886Q) /CSM/AF/AFO8-D/AFO886Q.Seq.d/                       1316   0.0
AFO491 (AFO491Q) /CSM/AF/AFO4-D/AFO491Q.Seq.d/                       1316   0.0
AFI349 (AFI349Q) /CSM/AF/AFI3-C/AFI349Q.Seq.d/                       1316   0.0
AFF384 (AFF384Q) /CSM/AF/AFF3-D/AFF384Q.Seq.d/                       1316   0.0
VFM849 (VFM849Q) /CSM/VF/VFM8-C/VFM849Q.Seq.d/                       1298   0.0
AFL540 (AFL540Q) /CSM/AF/AFL5-B/AFL540Q.Seq.d/                       1257   0.0
SSG212 (SSG212Q) /CSM/SS/SSG2-A/SSG212Q.Seq.d/                       1251   0.0
AFL683 (AFL683Q) /CSM/AF/AFL6-D/AFL683Q.Seq.d/                       1237   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC115604|AC115604.2 Dictyostelium discoideum chromosome 2 map 4354771-4414991 strain AX4, complete sequence.  1043  0.0  5
AL929357|AL929357.1 Plasmodium falciparum strain 3D7, chromosome 9; segment 3/5.    42  0.005  11
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    36  0.007  12
AL031745|AL031745.8 Plasmodium falciparum DNA from MAL1P2.        32  0.009  13
AL034559|AL034559.4 Plasmodium falciparum MAL3P7.                 32  0.018  11
AL844507|AL844507.1 Plasmodium falciparum chromosome 8.           40  0.026  14
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    38  0.046  15
AE014840|AE014840.1 Plasmodium falciparum 3D7 chromosome 11 section 5 of 8 of the complete sequence.    32  0.046  11
AL844506|AL844506.1 Plasmodium falciparum chromosome  7.          32  0.059  16
Z99129|Z99129.1 Human DNA sequence from clone 425C14 on chromosome 6q22 Contains the HSF2 gene for Heat Shock Factor 2 (Heat Shock Transcription Factor 2, HSTF 2) and an unknown gene similar to the placental protein DIFF33 gene. Contains ESTs, STSs and GSSs.    40  0.064  6
dna update 2003. 9. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC115604_7(AC115604|pid:none) Dictyostelium discoideum chromosom...   364   e-162
AC116032_2(AC116032|pid:none) Dictyostelium discoideum chromosom...    72   1e-23
AC115682_10(AC115682|pid:none) Dictyostelium discoideum chromoso...   103   1e-20
(Q54FS1) RecName: Full=FNIP repeat-containing protein DDB_G02906...    55   2e-17
(Q94481) RecName: Full=FNIP repeat-containing protein cigB;            66   2e-16
AC116032_11(AC116032|pid:none) Dictyostelium discoideum chromoso...    88   6e-16
U66528_1(U66528|pid:none) Dictyostelium discoideum CigB (cigB) m...    57   4e-14
AC115599_48(AC115599|pid:none) Dictyostelium discoideum chromoso...    67   6e-12
AC115680_16(AC115680|pid:none) Dictyostelium discoideum chromoso...    57   6e-12
AC114265_1(AC114265|pid:none) Dictyostelium discoideum chromosom...    54   2e-11
protein update 2009. 4.24
PSORT 

 psg: 0.47  gvh: 0.29  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for AFK178 is cyt
5' end seq. ID AFK178F
5' end seq. 
>AFK178F.Seq
AAAAATGAATAAAGAAANANGAAAATGAACAAAATACATTTTGTCTCATTCATTCAACAC
AGGAAATAACATTTATTTGCACTTCATGTAAATTTTCACCTGCTTGCAATAAGTGTGTGG
TTTTAAAGGGTATTCACTTTGGTCATGATATTATAGAAATCGATGATGAAAGTGTTTTAC
CAATTTTAAAAGATTGAATGAAAAAACAATACCAAAAATTGATGAATTAATAAAAAACAA
TCAAGATTTACTTAAAGGTAATTTAACAAAATATGAAGAAATTAAAGAAAAACATAAATC
AAATTTACAAATTATCAGTGGCCAAATTATGAAATTACATACAATTTTGCAAACTGTTGA
AANTAATTNAAATCAACAATTAATTACAAATTTATATGAAAATACTGATATTAATGATGT
TTCC----------
Length of 5' end seq. 424
3' end seq. ID AFK178Z
3' end seq. 
>AFK178Z.Seq
----------TGATTATAATTTCATTGATGAAAGCCAAAATTTAAATGATAAACACATTC
AAATCATTAAAGATTCATATCAAAGTAAAAAGATTTTGACATGTCATTTTAGTGATTTGC
CAGATTATTCAAGTTCAAAGGTTACCATTTCAGAGTCATCATTTAATTCAATTAAAGATT
TGTTTAATTTAATAATTAGTATTGATAAATACCATGGACAATTGCCTCCTACTAATGAAC
ATTCACCTCTAAAACAGTTGTCAAGATATAGATGGGGTAAATTTATATTTACAGTTTACA
ATGATGGTGATAGTATACCAGATGATACAGAATCTCTTGCAATTGGAGAGGGACAGCGTC
TACCTAAAACCATGCCAAAATCAACCAAAAGATTATTATTGCCCAATGGATTTAATCAAT
CATTAATATCATTACCAAAAACTGTTAGATCACTTTACCTTTTTAACATTGGTCTTCAAT
TGACTGAGGCTTCAATTCCTGAAACTGTAGATACACTCTTCTTTTGTGATGGATTTAGTC
AAAAAAATTACAAAGGGAATAATTCCAAAATCAGTTAAAGAGGTGTTTTTCTATAATATA
GTTCAACCACTTGAAAAAGATTCTATCCCAAACACTGTTTCATTTGTGGGTCGTTCCCCT
TCCTTTAAACATCCTCTTGATGTTAAATCAATAAATCAAATTAA
Length of 3' end seq. 694
Connected seq. ID AFK178P
Connected seq. 
>AFK178P.Seq
AAAAATGAATAAAGAAANANGAAAATGAACAAAATACATTTTGTCTCATTCATTCAACAC
AGGAAATAACATTTATTTGCACTTCATGTAAATTTTCACCTGCTTGCAATAAGTGTGTGG
TTTTAAAGGGTATTCACTTTGGTCATGATATTATAGAAATCGATGATGAAAGTGTTTTAC
CAATTTTAAAAGATTGAATGAAAAAACAATACCAAAAATTGATGAATTAATAAAAAACAA
TCAAGATTTACTTAAAGGTAATTTAACAAAATATGAAGAAATTAAAGAAAAACATAAATC
AAATTTACAAATTATCAGTGGCCAAATTATGAAATTACATACAATTTTGCAAACTGTTGA
AANTAATTNAAATCAACAATTAATTACAAATTTATATGAAAATACTGATATTAATGATGT
TTCC----------TGATTATAATTTCATTGATGAAAGCCAAAATTTAAATGATAAACAC
ATTCAAATCATTAAAGATTCATATCAAAGTAAAAAGATTTTGACATGTCATTTTAGTGAT
TTGCCAGATTATTCAAGTTCAAAGGTTACCATTTCAGAGTCATCATTTAATTCAATTAAA
GATTTGTTTAATTTAATAATTAGTATTGATAAATACCATGGACAATTGCCTCCTACTAAT
GAACATTCACCTCTAAAACAGTTGTCAAGATATAGATGGGGTAAATTTATATTTACAGTT
TACAATGATGGTGATAGTATACCAGATGATACAGAATCTCTTGCAATTGGAGAGGGACAG
CGTCTACCTAAAACCATGCCAAAATCAACCAAAAGATTATTATTGCCCAATGGATTTAAT
CAATCATTAATATCATTACCAAAAACTGTTAGATCACTTTACCTTTTTAACATTGGTCTT
CAATTGACTGAGGCTTCAATTCCTGAAACTGTAGATACACTCTTCTTTTGTGATGGATTT
AGTCAAAAAAATTACAAAGGGAATAATTCCAAAATCAGTTAAAGAGGTGTTTTTCTATAA
TATAGTTCAACCACTTGAAAAAGATTCTATCCCAAACACTGTTTCATTTGTGGGTCGTTC
CCCTTCCTTTAAACATCCTCTTGATGTTAAATCAATAAATCAAATTAA
Length of connected seq. 1118
Full length Seq ID -
Full length Seq. -
Length of full length seq. -