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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFK136
Library AF
(Link to library)
Clone ID AFK136
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10734-1
Original site URL
Representative seq. ID AFK136Z
(Link to Original site)
Representative DNA sequence 
>AFK136 (AFK136Q) /CSM/AF/AFK1-B/AFK136Q.Seq.d/
XXXXXXXXXXGTTGGGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTC
TCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAAT
TTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATT
GGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCA
GTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTT
TTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGAT
AAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGAC
TCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAA
GCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTT
GTTGAATTCATTCAAGATAACGCTGCCATNAATTCAAATTACCATCATCTCAAACTGANA
TAATGTNAATCTAAAAAAGATTCTTCGCTAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
---WVDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIG
GKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDK
LGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFV
EFIQDNAAXNSNYHHLKLX*cxskkdssl
Translated Amino Acid sequence (All Frames) 
Frame A:
---lgryeeipttsnsygslw*scsshlc*fscq*sslqfr*krnlliryrlqmdprchw
w*sfsic*istrtrik*css*gcrwyhfqkvgfrltkgcfg*ilrsmvrslqescsnlr*
ir*ilkrr*iclnrkd*crlk*ctk*yrn*rlpnhhalqs**qrksnll*rskk*syelc
*ihsr*rchxfklpssqtximxi*krffa


Frame B:
---WVDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIG
GKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDK
LGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFV
EFIQDNAAXNSNYHHLKLX*cxskkdssl


Frame C:
---gsi*rnshnkqliwvslvklfqpslliqlpiklvtismkkkpshsipspngskmslv
vkflhllnlnqnqnqmmlqlrlplvplskswf*thqrmfwlnstlhgaviarillqstin
*vnt*ktlnlsqs*rlmptqmmyqvisklevtqpscssklmtkkiqslmkvkemii*tll
nsfkitlpxiqitiisn*xnvnlkkilr*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFK858 (AFK858Q) /CSM/AF/AFK8-C/AFK858Q.Seq.d/                       1213   0.0
AFK136 (AFK136Q) /CSM/AF/AFK1-B/AFK136Q.Seq.d/                       1213   0.0
AFH744 (AFH744Q) /CSM/AF/AFH7-B/AFH744Q.Seq.d/                       1213   0.0
AFL143 (AFL143Q) /CSM/AF/AFL1-B/AFL143Q.Seq.d/                       1203   0.0
AFL139 (AFL139Q) /CSM/AF/AFL1-B/AFL139Q.Seq.d/                       1203   0.0
AFK470 (AFK470Q) /CSM/AF/AFK4-C/AFK470Q.Seq.d/                       1199   0.0
AFJ889 (AFJ889Q) /CSM/AF/AFJ8-D/AFJ889Q.Seq.d/                       1199   0.0
AFJ339 (AFJ339Q) /CSM/AF/AFJ3-B/AFJ339Q.Seq.d/                       1197   0.0
AFH538 (AFH538Q) /CSM/AF/AFH5-B/AFH538Q.Seq.d/                       1191   0.0
AFO713 (AFO713Q) /CSM/AF/AFO7-A/AFO713Q.Seq.d/                       1189   0.0
own update 2003. 5.15
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end, single read.   599  e-167  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74  2e-09  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.    70  4e-08  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70  4e-08  1
BM280519|BM280519.1 ki01h03.y1 Ascaris suum L4 pSPORT1 Zarlenga v1 Ascaris suum cDNA 5' similar to SW:ERP5_CAEEL Q11067 PROBABLE PROTEIN DISULFIDE ISOMERASE P5 PRECURSOR ;, mRNA sequence.    68  1e-07  1
AW255071|AW255071.1 ML1365 peppermint glandular trichome Mentha x piperita cDNA, mRNA sequence.    64  2e-06  1
BM315730|BM315730.1 fw64d09.y1 Sugano SJD adult male Danio rerio cDNA clone 5612584 5' similar to SW:ER60_HUMAN P30101 PROBABLE PROTEIN DISULFIDE ISOMERASE ER-60 PRECURSOR ;, mRNA sequence.    62  9e-06  1
BU795021|S34275 protein disulfide-isomerase homolog precursor - fluke  (Schistosoma mansoni), mRNA sequence.    60  4e-05  1
BM308748|BM308748.1 sak49h10.y1 Gm-c1036 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1036-6523 5' similar to TR:P93358 P93358 PROTEIN DISULFIDE-ISOMERASE PRECURSOR. ;, mRNA sequence.    60  4e-05  1
BM174369|PDA4_MOUSE PROTEIN DISULFIDE ISOMERASE A4 PRECURSOR (PROTEIN ERP-72) (ERP72), mRNA sequence.    60  4e-05  1
dna update 2003. 9. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   165   8e-40
(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   164   1e-39
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   161   2e-38
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   159   6e-38
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   154   2e-36
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   152   6e-36
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   151   2e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   149   5e-35
BC046707_1(BC046707|pid:none) Xenopus laevis glucose regulated p...   149   5e-35
(P13667) RecName: Full=Protein disulfide-isomerase A4;          ...   149   5e-35
protein update 2009. 4.24
PSORT 

 psg: 0.84  gvh: 0.38  alm: 0.43  top: 0.43  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 32.0 %: cytoplasmic
	 20.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for AFK136 is nuc
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFK136Z
3' end seq. 
>AFK136Z.Seq
----------GTTGGGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTC
TCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAAT
TTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATT
GGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCA
GTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTT
TTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGAT
AAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGAC
TCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAA
GCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTT
GTTGAATTCATTCAAGATAACGCTGCCATNAATTCAAATTACCATCATCTCAAACTGANA
TAATGTNAATCTAAAAAAGATTCTTCGCTAA
Length of 3' end seq. 621
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -