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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLB334
Library SL
(Link to library)
Clone ID SLB334
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1
Original site URL
Representative seq. ID SLB334Z
(Link to Original site)
Representative DNA sequence 
>SLB334 (SLB334Q) /CSM/SL/SLB3-B/SLB334Q.Seq.d/
XXXXXXXXXXAACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAACGTTAAA
AACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCA
CCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATC
CATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACT
GAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCC
GCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATG
TGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGA
CAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGT
GATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATAAATAAATAATATTTCTTAATAA
ATAGCATTTTATAATA
sequence update 1998. 4. 2
Translated Amino Acid sequence 
---QLPEARPGDNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVLNHPGQIH
AGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPMC
VESFTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK*innis**i
afyn
Translated Amino Acid sequence (All Frames) 
Frame A:
---ttprspsr*qcrfqr*krfsqrn*krygrw*lqkrsttrn*kircssyrpqpsrsnp
cwlltslrlshcshcl*ih*nrr*s*ssywcrrcqrrycrrrlkew*crygrinpissnv
c*ilh*iptirsfrrqryetnrrrrchqinrqespr*sr**ercccpikkeink*yflin
sil*


Frame B:
---QLPEARPGDNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVLNHPGQIH
AGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPMC
VESFTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK*innis**i
afyn


Frame C:
---nsqkpvqvtm*vstlktfqskklkevwslvtpktihhkklknsllklssstiqvksm
lvthqs*ivtlltlpvnslkssiklivvlvpslpkkvlppss*rmvmplwsn*phlvqcv
lnpslnthh*vvspsei*dkpspsvssnqpsrkpqvkpvirkvllphqkrnk*iiflnk*
hfii
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSE380 (VSE380Q) /CSM/VS/VSE3-D/VSE380Q.Seq.d/                       1082   0.0
VFN472 (VFN472Q) /CSM/VF/VFN4-C/VFN472Q.Seq.d/                       1082   0.0
SLK447 (SLK447Q) /CSM/SL/SLK4-B/SLK447Q.Seq.d/                       1082   0.0
SLH395 (SLH395Q) /CSM/SL/SLH3-D/SLH395Q.Seq.d/                       1082   0.0
SLE701 (SLE701Q) /CSM/SL/SLE7-A/SLE701Q.Seq.d/                       1082   0.0
SLE555 (SLE555Q) /CSM/SL/SLE5-C/SLE555Q.Seq.d/                       1082   0.0
SLE403 (SLE403Q) /CSM/SL/SLE4-A/SLE403Q.Seq.d/                       1082   0.0
SLE239 (SLE239Q) /CSM/SL/SLE2-B/SLE239Q.Seq.d/                       1082   0.0
SLE193 (SLE193Q) /CSM/SL/SLE1-D/SLE193Q.Seq.d/                       1082   0.0
SLE138 (SLE138Q) /CSM/SL/SLE1-B/SLE138Q.Seq.d/                       1082   0.0
own update 2001.11.27
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(X55973) D. discoideum EF1-I gene for elongation factor 1 al...   983   0.0    1
(AU285021) Dictyostelium discoideum gamete cDNA clone:FCL-AA...   983   0.0    1
(AU284628) Dictyostelium discoideum gamete cDNA clone:FC-BF1...   983   0.0    1
(AU284420) Dictyostelium discoideum gamete cDNA clone:FC-AT2...   983   0.0    1
(AU284342) Dictyostelium discoideum gamete cDNA clone:FC-AN0...   983   0.0    1
(AU284225) Dictyostelium discoideum gamete cDNA clone:FC-AA2...   983   0.0    1
(AU264545) Dictyostelium discoideum vegetative cDNA clone:VS...   983   0.0    1
(AU264497) Dictyostelium discoideum vegetative cDNA clone:VS...   983   0.0    1
(AU263935) Dictyostelium discoideum vegetative cDNA clone:VS...   983   0.0    1
(AU263821) Dictyostelium discoideum vegetative cDNA clone:VS...   983   0.0    1
dna update 2009. 2. 2
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   298   5e-80
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   298   5e-80
(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   298   5e-80
AY542532_1(AY542532|pid:none) Cladonema radiatum translation elo...   227   1e-58
AJ581004_1(AJ581004|pid:none) Axinella verrucosa mRNA for elonga...   225   7e-58
(Q00251) RecName: Full=Elongation factor 1-alpha;          Short...   224   1e-57
AY582829_1(AY582829|pid:none) Acanthamoeba culbertsoni ef1a gene...   224   1e-57
AY643820_1(AY643820|pid:none) Arcyria denudata elongation factor...   224   1e-57
AJ549292_1(AJ549292|pid:none) Podocoryne carnea mRNA for elongat...   223   2e-57
Y09023_1(Y09023|pid:none) G.cydonium mRNA for elongation factor ...   223   3e-57
protein update 2009. 6. 1
PSORT 

 psg: 0.75  gvh: 0.50  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 28.0 %: nuclear
	 24.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for SLB334 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID SLB334Z
3' end seq. 
>SLB334Z.Seq
----------AACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAACGTTAAA
AACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCA
CCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATC
CATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACT
GAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCC
GCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATG
TGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGA
CAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGT
GATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATAAATAAATAATATTTCTTAATAA
ATAGCATTTTATAATA
Length of 3' end seq. 546
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -