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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLB314
Library SL
(Link to library)
Clone ID SLB314
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1
Original site URL
Representative seq. ID SLB314Z
(Link to Original site)
Representative DNA sequence 
>SLB314 (SLB314Q) /CSM/SL/SLB3-A/SLB314Q.Seq.d/
XXXXXXXXXXTACTGTTCCAGTAGGTCGTGTCGAAACTGGTATCATTAAACCAGGTATGG
TCGTCACCTTTGCCCCAGCTGGTCTCTCAACTGAAGTCAAATCAGTCGAAATGCATCACG
AACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAACGTTAAAAACGTTTCAG
TCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCACCACAAGAAA
CTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCCATGCTGGTT
ACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCG
ATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCT
TAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAAT
CCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTCG
CCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTGATAAGAAAG
GTGCTGCTGCCCCATCAAAAAAGAAATAAATAAATAATATTTCTTAATAAATAGCATTTT
ATAATAAAAA
sequence update 1998. 4. 2
Translated Amino Acid sequence 
---TVPVGRVETGIIKPGMVVTFAPAGLSTEVKSVEMHHEQLPEARPGDNVGFNVKNVSV
KEIKRGMVAGDSKNDPPQETEKFVAQVIVLNHPGQIHAGYSPVLDCHTAHIACKFTEIVD
KVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPMCVESFTEYPPLGRFAVRDMRQTVA
VGVIKSTVKKAPGKAGDKKGAAAPSKKK*innis**iafynk
Translated Amino Acid sequence (All Frames) 
Frame A:
---TVPVGRVETGIIKPGMVVTFAPAGLSTEVKSVEMHHEQLPEARPGDNVGFNVKNVSV
KEIKRGMVAGDSKNDPPQETEKFVAQVIVLNHPGQIHAGYSPVLDCHTAHIACKFTEIVD
KVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPMCVESFTEYPPLGRFAVRDMRQTVA
VGVIKSTVKKAPGKAGDKKGAAAPSKKK*innis**iafynk


Frame B:
---lfq*vvsklvslnqvwssplpqlvsqlksnqskcitnnsqkpvqvtm*vstlktfqs
kklkevwslvtpktihhkklknsllklssstiqvksmlvthqs*ivtlltlpvnslkssi
klivvlvpslpkkvlppss*rmvmplwsn*phlvqcvlnpslnthh*vvspsei*dkpsp
svssnqpsrkpqvkpvirkvllphqkrnk*iiflnk*hfiik


Frame C:
---cssrscrnwyh*trygrhlcpswsln*sqisrnasrttprspsr*qcrfqr*krfsq
rn*krygrw*lqkrsttrn*kircssyrpqpsrsnpcwlltslrlshcshcl*ih*nrr*
s*ssywcrrcqrrycrrrlkew*crygrinpissnvc*ilh*iptirsfrrqryetnrrr
rchqinrqespr*sr**ercccpikkeink*yflinsil**k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD740 (VSD740Q) /CSM/VS/VSD7-B/VSD740Q.Seq.d/                       1199   0.0
VSD380 (VSD380Q) /CSM/VS/VSD3-D/VSD380Q.Seq.d/                       1199   0.0
VSD312 (VSD312Q) /CSM/VS/VSD3-A/VSD312Q.Seq.d/                       1199   0.0
VFO616 (VFO616Q) /CSM/VF/VFO6-A/VFO616Q.Seq.d/                       1199   0.0
VFN666 (VFN666Q) /CSM/VF/VFN6-C/VFN666Q.Seq.d/                       1199   0.0
VFN474 (VFN474Q) /CSM/VF/VFN4-D/VFN474Q.Seq.d/                       1199   0.0
VFN376 (VFN376Q) /CSM/VF/VFN3-D/VFN376Q.Seq.d/                       1199   0.0
VFN329 (VFN329Q) /CSM/VF/VFN3-B/VFN329Q.Seq.d/                       1199   0.0
VFN318 (VFN318Q) /CSM/VF/VFN3-A/VFN318Q.Seq.d/                       1199   0.0
VFN184 (VFN184Q) /CSM/VF/VFN1-D/VFN184Q.Seq.d/                       1199   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(X55973) D. discoideum EF1-I gene for elongation factor 1 al...  1201   0.0    1
(AU284628) Dictyostelium discoideum gamete cDNA clone:FC-BF1...  1201   0.0    1
(AU284342) Dictyostelium discoideum gamete cDNA clone:FC-AN0...  1201   0.0    1
(AU264497) Dictyostelium discoideum vegetative cDNA clone:VS...  1201   0.0    1
(AU263935) Dictyostelium discoideum vegetative cDNA clone:VS...  1201   0.0    1
(AU263821) Dictyostelium discoideum vegetative cDNA clone:VS...  1201   0.0    1
(AU060206) Dictyostelium discoideum slug cDNA, clone SLA573.     1201   0.0    1
(AU052275) Dictyostelium discoideum slug cDNA, clone SLA488.     1201   0.0    1
(AU052253) Dictyostelium discoideum slug cDNA, clone SLA434.     1201   0.0    1
(AU052232) Dictyostelium discoideum slug cDNA, clone SLA286.     1201   0.0    1
dna update 2009. 2. 2
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   371   e-101
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   371   e-101
(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   371   e-101
AY542532_1(AY542532|pid:none) Cladonema radiatum translation elo...   293   3e-78
(P41745) RecName: Full=Elongation factor 1-alpha;          Short...   291   9e-78
AY643816_1(AY643816|pid:none) Lycogala epidendrum elongation fac...   290   3e-77
Y09023_1(Y09023|pid:none) G.cydonium mRNA for elongation factor ...   290   3e-77
AJ581004_1(AJ581004|pid:none) Axinella verrucosa mRNA for elonga...   289   6e-77
(Q00251) RecName: Full=Elongation factor 1-alpha;          Short...   289   6e-77
AY582829_1(AY582829|pid:none) Acanthamoeba culbertsoni ef1a gene...   289   6e-77
protein update 2009. 6. 1
PSORT 

 psg: 0.79  gvh: 0.50  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for SLB314 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID SLB314Z
3' end seq. 
>SLB314Z.Seq
----------TACTGTTCCAGTAGGTCGTGTCGAAACTGGTATCATTAAACCAGGTATGG
TCGTCACCTTTGCCCCAGCTGGTCTCTCAACTGAAGTCAAATCAGTCGAAATGCATCACG
AACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAACGTTAAAAACGTTTCAG
TCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCACCACAAGAAA
CTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCCATGCTGGTT
ACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCG
ATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCT
TAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAAT
CCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTCG
CCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTGATAAGAAAG
GTGCTGCTGCCCCATCAAAAAAGAAATAAATAAATAATATTTCTTAATAAATAGCATTTT
ATAATAAAAA
Length of 3' end seq. 660
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -