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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SLB265
Library SL
(Link to library)
Clone ID SLB265
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1
Original site URL
Representative seq. ID SLB265Z
(Link to Original site)
Representative DNA sequence 
>SLB265 (SLB265Q) /CSM/SL/SLB2-C/SLB265Q.Seq.d/
XXXXXXXXXXGTATGGTCGTCACCTTTGCCCCAGCTGGTCTCTCAACTGAAGTCAAATCA
GTCGAAATGCATCACGAACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAAC
GTTAAAAACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAAC
GATCCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGT
CAAATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAA
TTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGT
ACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGT
CCAATGTGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGAT
ATGAGACAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAA
GCCGGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATAAATAAATAATATTTCT
TAATAAATAGCATTTTATAATAATTTTAAAAAAAAAAAAAAA
sequence update 1998. 3.28
Translated Amino Acid sequence 
---MVVTFAPAGLSTEVKSVEMHHEQLPEARPGDNVGFNVKNVSVKEIKRGMVAGDSKND
PPQETEKFVAQVIVLNHPGQIHAGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGT
AAVVLKNGDAAMVELTPSRPMCVESFTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKA
GDKKGAAAPSKKK*innis**iafynnfkkkkk
Translated Amino Acid sequence (All Frames) 
Frame A:
---ygrhlcpswsln*sqisrnasrttprspsr*qcrfqr*krfsqrn*krygrw*lqkr
sttrn*kircssyrpqpsrsnpcwlltslrlshcshcl*ih*nrr*s*ssywcrrcqrry
crrrlkew*crygrinpissnvc*ilh*iptirsfrrqryetnrrrrchqinrqespr*s
r**ercccpikkeink*yflinsil**f*kkkk


Frame B:
---MVVTFAPAGLSTEVKSVEMHHEQLPEARPGDNVGFNVKNVSVKEIKRGMVAGDSKND
PPQETEKFVAQVIVLNHPGQIHAGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGT
AAVVLKNGDAAMVELTPSRPMCVESFTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKA
GDKKGAAAPSKKK*innis**iafynnfkkkkk


Frame C:
---wssplpqlvsqlksnqskcitnnsqkpvqvtm*vstlktfqskklkevwslvtpkti
hhkklknsllklssstiqvksmlvthqs*ivtlltlpvnslkssiklivvlvpslpkkvl
ppss*rmvmplwsn*phlvqcvlnpslnthh*vvspsei*dkpspsvssnqpsrkpqvkp
virkvllphqkrnk*iiflnk*hfiiilkkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSE380 (VSE380Q) /CSM/VS/VSE3-D/VSE380Q.Seq.d/                       1223   0.0
SLE701 (SLE701Q) /CSM/SL/SLE7-A/SLE701Q.Seq.d/                       1223   0.0
SLE555 (SLE555Q) /CSM/SL/SLE5-C/SLE555Q.Seq.d/                       1223   0.0
SLE403 (SLE403Q) /CSM/SL/SLE4-A/SLE403Q.Seq.d/                       1223   0.0
SLE193 (SLE193Q) /CSM/SL/SLE1-D/SLE193Q.Seq.d/                       1223   0.0
SLE138 (SLE138Q) /CSM/SL/SLE1-B/SLE138Q.Seq.d/                       1223   0.0
SLC291 (SLC291Q) /CSM/SL/SLC2-D/SLC291Q.Seq.d/                       1223   0.0
SLC219 (SLC219Q) /CSM/SL/SLC2-A/SLC219Q.Seq.d/                       1223   0.0
SLC166 (SLC166Q) /CSM/SL/SLC1-C/SLC166Q.Seq.d/                       1223   0.0
SLC120 (SLC120Q) /CSM/SL/SLC1-A/SLC120Q.Seq.d/                       1223   0.0
own update 2000. 8.26
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(X55973) D. discoideum EF1-I gene for elongation factor 1 al...  1112   0.0    1
(AU284628) Dictyostelium discoideum gamete cDNA clone:FC-BF1...  1112   0.0    1
(AU284342) Dictyostelium discoideum gamete cDNA clone:FC-AN0...  1112   0.0    1
(AU264497) Dictyostelium discoideum vegetative cDNA clone:VS...  1112   0.0    1
(AU263935) Dictyostelium discoideum vegetative cDNA clone:VS...  1112   0.0    1
(AU263821) Dictyostelium discoideum vegetative cDNA clone:VS...  1112   0.0    1
(AU263066) Dictyostelium discoideum vegetative cDNA clone:VS...  1112   0.0    1
(AU060206) Dictyostelium discoideum slug cDNA, clone SLA573.     1112   0.0    1
(AU052275) Dictyostelium discoideum slug cDNA, clone SLA488.     1112   0.0    1
(AU052273) Dictyostelium discoideum slug cDNA, clone SLA482.     1112   0.0    1
dna update 2009. 2. 2
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   341   9e-93
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   341   9e-93
(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   341   9e-93
(Q00251) RecName: Full=Elongation factor 1-alpha;          Short...   265   1e-69
AY542532_1(AY542532|pid:none) Cladonema radiatum translation elo...   263   2e-69
(P41745) RecName: Full=Elongation factor 1-alpha;          Short...   262   5e-69
AJ549292_1(AJ549292|pid:none) Podocoryne carnea mRNA for elongat...   261   1e-68
Y09023_1(Y09023|pid:none) G.cydonium mRNA for elongation factor ...   261   1e-68
AY643816_1(AY643816|pid:none) Lycogala epidendrum elongation fac...   261   2e-68
AY582829_1(AY582829|pid:none) Acanthamoeba culbertsoni ef1a gene...   260   2e-68
protein update 2009. 6. 1
PSORT 

 psg: 0.93  gvh: 0.41  alm: 0.45  top: 0.33  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SLB265 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID SLB265Z
3' end seq. 
>SLB265Z.Seq
----------GTATGGTCGTCACCTTTGCCCCAGCTGGTCTCTCAACTGAAGTCAAATCA
GTCGAAATGCATCACGAACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAAC
GTTAAAAACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAAC
GATCCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGT
CAAATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAA
TTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGT
ACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGT
CCAATGTGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGAT
ATGAGACAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAA
GCCGGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATAAATAAATAATATTTCT
TAATAAATAGCATTTTATAATAATTTTAAAAAAAAAAAAAAA
Length of 3' end seq. 632
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -