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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFK117
Library AF
(Link to library)
Clone ID AFK117
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16513-1
Original site URL
Representative seq. ID AFK117P
(Link to Original site)
Representative DNA sequence 
>AFK117 (AFK117Q) /CSM/AF/AFK1-A/AFK117Q.Seq.d/
CCAAAGTTGATTGTGACCAAGCTGACAACAAAGCTCTCTGTAGTAAATATGATGTTTCTG
GTTATCCAACCNTCAAAATCTTTGACAAATCAACCACTGCCAAAGATTACAACGGTGCTA
GATCAGNNGATGAATTATTAACTTATATCAATAATCACNCCAAAACCAACGNTNAAGNNA
NGAANGCACCNTNNNAXXXXXXXXXXTCNAATGTTGTTGATTTATCNCCATCAAATTTTG
ATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTAGTTGAATTCTATGCACCATGGTGTG
GTCATTGCAAAAAGTTAATGCCAGATTATGAAATCCTTGGTAACACCTACGCCAATGAAA
AAGATGTTGTCATCGCCAAAATTGATTGCGATGCTGCTGATAACAAAGCCATCTGCTCCA
AATATGGTGTCACTGGTTTCCCAACACTCAAATGGTTCGGTAAACAAAGCAAAGACGGTG
AAAAATATGAACAAGGTCGTGATCTTGATACTTTCATCAACTACATCAACAAACAAGCTG
GTGTTAACCGTGTCAAAGGTGGTAAACTCGCTGTTGGTGCTGGTCGTGTTGAACAACTCG
ATACTATTGCCACTGAATTCATCGCTGCCGCTGCTGAAGTCCGTAAGGAACTCGTCAAGA
AAGCTCAAACCGTCGTTGATTCTCTCCCAGAAGAATTAAGAACTGAAGGTTCATACTACG
TCAAAGTCATGAAAACCATTGCTGAAAAATCAATCGATTTTGTAACCACTGAAATCGCTC
GTATCACCAAATTAGTCTCTGGTTCAATGTCTGGAAAGAAAGCTGATGAATTTGCCAAGA
AACTCAATATTTTAGAATCTTTCAAATCAAAATAAATTAATTTATTAGAAAATAAAAATA
AATAAAGAAAAAAAAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
KVDCDQADNKALCSKYDVSGYPTXKIFDKSTTAKDYNGARSXDELLTYINNHXKTNXXXX
XAPX---

---SNVVDLSPSNFDSVVLDKSKNVLVEFYAPWCGHCKKLMPDYEILGNTYANEKDVVIA
KIDCDAADNKAICSKYGVTGFPTLKWFGKQSKDGEKYEQGRDLDTFINYINKQAGVNRVK
GGKLAVGAGRVEQLDTIATEFIAAAAEVRKELVKKAQTVVDSLPEELRTEGSYYVKVMKT
IAEKSIDFVTTEIARITKLVSGSMSGKKADEFAKKLNILESFKSK*inllenknk*rkk
Translated Amino Acid sequence (All Frames) 
Frame A:
pklivtklttklsvvnmmflviqpsksltnqplpkittvldqxmny*lisiitpkptxkx
xxhxx---

---SNVVDLSPSNFDSVVLDKSKNVLVEFYAPWCGHCKKLMPDYEILGNTYANEKDVVIA
KIDCDAADNKAICSKYGVTGFPTLKWFGKQSKDGEKYEQGRDLDTFINYINKQAGVNRVK
GGKLAVGAGRVEQLDTIATEFIAAAAEVRKELVKKAQTVVDSLPEELRTEGSYYVKVMKT
IAEKSIDFVTTEIARITKLVSGSMSGKKADEFAKKLNILESFKSK*inllenknk*rkk

Frame B:
qs*l*ps*qqssl**i*cfwlsnxqnl*qinhcqrlqrc*ixx*iinlyq*sxqnqrxxx
extxx---

---xmlliyxhqiliqlf*inqrmf*lnsmhhgvviaks*cqimkslvtptpmkkmlssp
kliamllitkpsapnmvslvsqhsngsvnkaktvknmnkvvililssttstnklvltvsk
vvnsllvlvvlnnsillplnsslpllksvrnssrklkpslilsqkn*elkvhttsks*kp
llknqsil*plkslvspn*slvqclerklmnlprnsif*nlsnqnkliy*kikinkekk

Frame C:
KVDCDQADNKALCSKYDVSGYPTXKIFDKSTTAKDYNGARSXDELLTYINNHXKTNXXXX
XAPX---

---xcc*fixikf*fscfr*ikecfs*ilctmvwslqkvnarl*npw*hlrq*krcchrq
n*lrcc**qshllqiwchwfpntqmvr*tkqrr*ki*trs*s*yfhqlhqqtswc*pcqr
w*trcwcwsc*ttryych*ihrcrc*sp*gtrqessnrr*fsprrikn*rfilrqshenh
c*kinrfcnh*nrsyhqislwfnvwkes**icqetqyfrifqikin*firk*k*ikkkn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO821 (VFO821Q) /CSM/VF/VFO8-A/VFO821Q.Seq.d/                       1300   0.0
VFO241 (VFO241Q) /CSM/VF/VFO2-B/VFO241Q.Seq.d/                       1300   0.0
VFN359 (VFN359Q) /CSM/VF/VFN3-C/VFN359Q.Seq.d/                       1300   0.0
VFN233 (VFN233Q) /CSM/VF/VFN2-B/VFN233Q.Seq.d/                       1300   0.0
VFN206 (VFN206Q) /CSM/VF/VFN2-A/VFN206Q.Seq.d/                       1300   0.0
VFM578 (VFM578Q) /CSM/VF/VFM5-D/VFM578Q.Seq.d/                       1300   0.0
VFM537 (VFM537Q) /CSM/VF/VFM5-B/VFM537Q.Seq.d/                       1300   0.0
VFM151 (VFM151Q) /CSM/VF/VFM1-C/VFM151Q.Seq.d/                       1300   0.0
VFL481 (VFL481Q) /CSM/VF/VFL4-D/VFL481Q.Seq.d/                       1300   0.0
VFL370 (VFL370Q) /CSM/VF/VFL3-C/VFL370Q.Seq.d/                       1300   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116988|AC116988.2 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.  1300  0.0  6
AF019112|AF019112.1 Dictyostelium discoideum protein disulfide isomerase (dis) mRNA, complete cds.  1292  0.0  2
CB090389|CB090389.1 gy74e10.g1 Cycad Leaf Library (NYBG) Cycas rumphii cDNA clone gy74e10, mRNA sequence.    80  6e-13  2
CB091549|CB091549.1 he93f09.g1 Cycad Leaf Library (NYBG) Cycas rumphii cDNA clone he93f09, mRNA sequence.    80  6e-11  1
AW290132|AW290132.1 NXNV012E07F Nsf Xylem Normal wood Vertical Pinus taeda cDNA clone NXNV012E07 5', mRNA sequence.    58  3e-10  2
CD026805|CD026805.1 NXNV012E07 Nsf Xylem Normal wood Vertical Pinus taeda cDNA clone NXNV012E07 5' similar to Arabidopsis thaliana sequence At2g47470 putative protein disulfide-isomerase see http://mips.gsf.de/proj/thal/db/index.html, mRNA sequence.    58  3e-10  2
BX252003|BX252003.1 Pinus pinaster xylem EST, clone PP058D07.     50  4e-10  3
BQ695627|BQ695627.1 NXPV_030_G06_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda cDNA clone NXPV_030_G06 5', mRNA sequence.    58  4e-10  2
CD266987|CD266987.1 tab06g03.x1 Hydra EST -III Hydra magnipapillata cDNA 3' similar to TR:Q17770 Q17770 CODED FOR BY C. ELEGANS CDNA YK81H6.5. [1] ;, mRNA sequence.    66  1e-07  2
CD267971|CD267971.1 tab13b10.x1 Hydra EST -III Hydra magnipapillata cDNA 3' similar to SW:PDI_CHICK P09102 PROTEIN DISULFIDE ISOMERASE ;, mRNA sequence.    66  1e-07  2
dna update 2003. 9. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86IA3) RecName: Full=Protein disulfide-isomerase 1;          S...   515   e-145
AF019112_1(AF019112|pid:none) Dictyostelium discoideum protein d...   513   e-144
EF086125_1(EF086125|pid:none) Picea sitchensis clone WS0285_I21 ...   236   1e-60
EU284948_1(EU284948|pid:none) TSA: Elaeis guineensis EgEfMPOB001...   229   9e-59
DQ222488_1(DQ222488|pid:none) Solanum tuberosum clone 098B03 dis...   228   3e-58
AC087425_4(AC087425|pid:none) Oryza sativa (japonica cultivar-gr...   227   5e-58
(O22263) RecName: Full=Probable protein disulfide-isomerase A6; ...   226   6e-58
T03644(T03644) probable protein disulfide-isomerase (EC 5.3.4.1)...   224   3e-57
AM485099_1(AM485099|pid:none) Vitis vinifera contig VV78X160692....   224   3e-57
AB182629_1(AB182629|pid:none) Glycine max Gm pdis-1a mRNA for pr...   224   4e-57
protein update 2009. 4.24
PSORT 

 psg: 0.75  gvh: 0.31  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 36.0 %: cytoplasmic
	 20.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for AFK117 is nuc
5' end seq. ID AFK117F
5' end seq. 
>AFK117F.Seq
CCAAAGTTGATTGTGACCAAGCTGACAACAAAGCTCTCTGTAGTAAATATGATGTTTCTG
GTTATCCAACCNTCAAAATCTTTGACAAATCAACCACTGCCAAAGATTACAACGGTGCTA
GATCAGNNGATGAATTATTAACTTATATCAATAATCACNCCAAAACCAACGNTNAAGNNA
NGAANGCACCNTNNNA----------
Length of 5' end seq. 196
3' end seq. ID AFK117Z
3' end seq. 
>AFK117Z.Seq
----------TCNAATGTTGTTGATTTATCNCCATCAAATTTTGATTCAGTTGTTTTAGA
TAAATCAAAGAATGTTTTAGTTGAATTCTATGCACCATGGTGTGGTCATTGCAAAAAGTT
AATGCCAGATTATGAAATCCTTGGTAACACCTACGCCAATGAAAAAGATGTTGTCATCGC
CAAAATTGATTGCGATGCTGCTGATAACAAAGCCATCTGCTCCAAATATGGTGTCACTGG
TTTCCCAACACTCAAATGGTTCGGTAAACAAAGCAAAGACGGTGAAAAATATGAACAAGG
TCGTGATCTTGATACTTTCATCAACTACATCAACAAACAAGCTGGTGTTAACCGTGTCAA
AGGTGGTAAACTCGCTGTTGGTGCTGGTCGTGTTGAACAACTCGATACTATTGCCACTGA
ATTCATCGCTGCCGCTGCTGAAGTCCGTAAGGAACTCGTCAAGAAAGCTCAAACCGTCGT
TGATTCTCTCCCAGAAGAATTAAGAACTGAAGGTTCATACTACGTCAAAGTCATGAAAAC
CATTGCTGAAAAATCAATCGATTTTGTAACCACTGAAATCGCTCGTATCACCAAATTAGT
CTCTGGTTCAATGTCTGGAAAGAAAGCTGATGAATTTGCCAAGAAACTCAATATTTTAGA
ATCTTTCAAATCAAAATAAATTAATTTATTAGAAAATAAAAATAAATAAAGAAAAAAAAT
Length of 3' end seq. 710
Connected seq. ID AFK117P
Connected seq. 
>AFK117P.Seq
CCAAAGTTGATTGTGACCAAGCTGACAACAAAGCTCTCTGTAGTAAATATGATGTTTCTG
GTTATCCAACCNTCAAAATCTTTGACAAATCAACCACTGCCAAAGATTACAACGGTGCTA
GATCAGNNGATGAATTATTAACTTATATCAATAATCACNCCAAAACCAACGNTNAAGNNA
NGAANGCACCNTNNNA----------TCNAATGTTGTTGATTTATCNCCATCAAATTTTG
ATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTAGTTGAATTCTATGCACCATGGTGTG
GTCATTGCAAAAAGTTAATGCCAGATTATGAAATCCTTGGTAACACCTACGCCAATGAAA
AAGATGTTGTCATCGCCAAAATTGATTGCGATGCTGCTGATAACAAAGCCATCTGCTCCA
AATATGGTGTCACTGGTTTCCCAACACTCAAATGGTTCGGTAAACAAAGCAAAGACGGTG
AAAAATATGAACAAGGTCGTGATCTTGATACTTTCATCAACTACATCAACAAACAAGCTG
GTGTTAACCGTGTCAAAGGTGGTAAACTCGCTGTTGGTGCTGGTCGTGTTGAACAACTCG
ATACTATTGCCACTGAATTCATCGCTGCCGCTGCTGAAGTCCGTAAGGAACTCGTCAAGA
AAGCTCAAACCGTCGTTGATTCTCTCCCAGAAGAATTAAGAACTGAAGGTTCATACTACG
TCAAAGTCATGAAAACCATTGCTGAAAAATCAATCGATTTTGTAACCACTGAAATCGCTC
GTATCACCAAATTAGTCTCTGGTTCAATGTCTGGAAAGAAAGCTGATGAATTTGCCAAGA
AACTCAATATTTTAGAATCTTTCAAATCAAAATAAATTAATTTATTAGAAAATAAAAATA
AATAAAGAAAAAAAAT
Length of connected seq. 906
Full length Seq ID -
Full length Seq. -
Length of full length seq. -